introduction to electron spin resonance and spin trapping michael r. gunther west virginia...
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Introduction to Electron Spin Resonance and Spin Trapping
Michael R. Gunther
West Virginia University School of Medicine
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Free Radicals and EPR
• Molecules with one or more unpaired electron– Quantum mechanics: unpaired electrons have
spin and charge and hence magnetic moment– Electronic spin can be in either of two
directions (formally up or down)– The two spin states under normal conditions
are energetically degenerate– Energetic degeneracy lost when exposed to
an external magnetic field
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The EPR experiment
• Put sample into experimental magnetic field (B)
• Irradiate (microwave frequencies)
• Measure absorbance of radiation as f(B)
Weil, Bolton, and Wertz, 1994, “Electron Paramagnetic Resonance”
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The EPR spectrometer
• Electromagnet• Microwave source
and detector (typically X band, ~9.5 GHz)
• Modulation of magnetic field and phase-sensitive detection
• Spectrum 1st derivative
Weil, Bolton, and Wertz, 1994, “Electron Paramagnetic Resonance”
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The EPR spectrum
• A 1st derivative spectrum is obtained from the unpaired electron
• h = gB0
• g is a characteristic of the chemical environment of the unpaired electron; for free radicals it is near 2.00; can vary widely for transition metal centers
• Complicated/enhanced by hyperfine interactions with nuclei with non-zero spin
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The hyperfine effect
• The magnetic field experienced by the unpaired electron is affected by nearby nuclei with non-zero nuclear spin
Weil, Bolton, and Wertz, 1994, “Electron Paramagnetic Resonance”, New York: Wiley Interscience.
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Hyperfine splitting of EPR spectra
• The magnitude of the splitting and the number of lines depend upon:– The nuclear spin of the interacting nucleus
• # of lines = 2n(I + ½) so I = ½ gives 2 lines, etc.
– The nuclear gyromagnetic ratio– The magnitude of the interaction between the
electronic spin and the nuclear spin• Magnitude of the splitting typically decreases
greatly with increasing numbers of bonds between the nucleus and unpaired electron
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10 Gauss
No hyperfine
1 I=1/2 nucleus (1H)
1 I=1 nucleus (14N)
2 identical I=1/2 nuclei
1 I=5/2 nucleus (17O)
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Hyperfine splittings are additive
1 N, = 14.9 Ga
1N, = 14.9 G, 1H, = 2.5 Ga a
1N, = 14.9 G, 1H, = 14.9 Ga a
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Direct EPR analysis of a radical
• Radical cannot be diatomic • Radical must be available at a detectable
concentration– At least metastable– Frozen solution to greatly decrease radical decay
• Can greatly complicate the spectrum due to anisotropy
– Continuous formation inside resonator• Enzymatic radical formation• Flow experiment
• Radical characterized by hyperfine analysis
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Direct EPR of a tyrosyl radical
• Gunther, M.R., Sturgeon, B.E., and Mason, R.P., Free Radic. Biol. Med. 28:709-719, 2000
H
HH
H
O.CH2HC
COR'
NHR
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Spin trapping: when direct EPR is not convenient or possible
• Unstable free radical reacts with diamagnetic molecule (the spin trap) to form a relatively stable free radical
• The vast majority of spin traps form radical adducts through the addition of the radical to the trap to form a nitroxide radical
• 2 major classes of traps: nitrones and nitroso compounds
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Advantages of the nitrones
• React with a variety of different free radicals to form nitroxide adducts– RC., RO., RS., in some cases RN.
• Adducts are often quite stable
• Not terribly toxic so amenable to in vivo/ex vivo spin trapping
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Nitrone spin traps
• DMPO, 5,5-dimethylpyrroline N-oxide
• PBN/4-POBN, phenyl-N-t-butylnitrone
N
H3C
H3C
H
O
N
H3C
H3C
H
RO.
R.
C
H
N C(CH3)3
O
C
H
N C(CH3)3
R
O.
R.
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EPR spectra from DMPO adducts
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EPR spectra from 4-POBN adducts
20 G auss
N C
H
N
O.
C(CH3)3
CR
O
N C
H
N
O.
C(CH3)3O
13CR
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Nitroso spin traps
• Free radical adds to the nitrogen atom of a C-nitroso compound
• 2-methyl-2-nitrosopropane, MNP
• 3,5-dibromo-4-nitrosobenzene sulfonate
NO C(CH3)3 N C(CH3)3.O
R
R.
NO
Br
Br
SO3- N
Br
Br
SO3-
R
.OR.
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EPR spectra from methyl radical adducts of nitroso traps
MNP/ CH3, = 17.2 G; = 14.2 G (3H) . aN aH
DBNBS/ CH3, = 14.3 G; = 13.3 G (3H). aN aH
40 Gauss
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DMPO-trapping the tyrosyl radical
Natural isotope abundance
17O-labeled tyrosine
• Oxidize tyrosine with HRP/H2O2
N
CH3
CH3
H
O
CH2R
O.
Gunther, M.R., et al., Biochem. J. 330:1293-1299, 1998.
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Spin trap-derived hyperfine from MNP and MNP-d9
• Each line in the EPR spectra from MNP adducts is broadened by hyperfine from the 9 equivalent protons on the spin trap
NR
O .
CC H 3
C H 3
C H 3
NR
O .
CC D 3
C D 3
C D 3
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MNP-trapping the tyrosyl radical
.O
H
H
H
H
C
H
H
CH
NH2
CO2-
MNP/ tyrosine.
MNP 9/ tyrosined .
N
O.
OH
H
CH2R
(CH3)3C
.O
H
H
H
H
CH
HCHNH2
CO2-
• Gunther, M.R., et al., Biochem. J. 330:1293-1299, 1998.
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Why not spin trap?
• Nitrone spin traps, especially DMPO– Adducts can interconvert, i.e., DMPO/.OOH decays to
form DMPO/.OH– Subject to rare nucleophilic addition across their
double bonds– Yields an EPR silent hydroxylamine which can be
facilely oxidized up to the nitroxide
N
O
H3C
H3C
H
N
O
H3C
H3C
H
OR
H
ROH
Men+- H+
- e-N
H3C
H3C
H
OR
O.
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Why not spin trap?
• Nitroso spin traps MNP and DBNBS– Often acutely toxic so can’t use in vivo– The C-nitroso group critical to their function is highly reactive– Tend to directly add across unsaturated systems giving EPR-silent
hydroxylamines that are readily oxidized to the corresponding nitroxides
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Summary
• The main feature of EPR spectra that is useful for assignment to a particular free radical structure is hyperfine splitting
• Direct EPR spectra can provide a wealth of structural information
• Highly unstable free radicals can, in many cases, be stabilized for EPR characterization by spin trapping– The increased stability of the detected free radical comes with a
loss of structural information– The adduct may undergo chemistry between formation and
detection– Adduct assignment is assisted by selective isotope labeling and
EPR analysis of an independent preparation of the suspected adduct
– The performance of appropriate controls is essential