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Biotechnology &Biochemical Engineering

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Objectives: To encourages the students to work in the field of bio-chemical Industry.

The objective of this program is to introduce the basic concepts of bio-processing to the chemical engineers.

Outcomes:

1. Student will get well acquainted with intervention of chemicalengineering principles to biological process.2. Student will able to understand the concepts of enzyme

technology, immobilization technology, cell growth kinetics.

3. Student will get knowledge of bioreactors, fermentation, sterilizationtechniques, and media preparation.

4. Student will get well acquainted with biological process specific reactordesign aspects.

5. Student will get knowledge of downstream processing in fermentationindustries.

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Books

1. Biochemical Engineering fundamentals ByBailey ollis

2. Bioprocess Engineering:-Basic concept by Shuler& Kargi (PHI)

3. Biochemical Engineering:-principles & concepts by Syed Tanveer Ahmed Inamdar(PHI)

4. Introduction to Biochemical Engineering by

D.G.Rao

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Introduction

Biotechnology :- fusion of biology & technology 

Biological agents

Production of useful products

Biochemical engineering  Application of chemical engineering principles to biological

systems

Biologically derived products

Scientific & engineering principle

Biological agents(enzyme ,cell or microbes)

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Benefits of Biotechnology 

Medicine Human

 Veterinary 

Biopharming

Environment

 Agriculture

Food products

Industry and manufacturing

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 Activities Involved in Bioprocess Industry 

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CELL

• Cell- a discrete unit of life

•Molecular biology- study of  molecules in cellsCells as reactors

•Metabolism- processes by   which organisms use nutrients

•Catabolism- breaking downmaterials into smallercomponents

• Anabolism- building tissuesfrom smaller materials

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Cell Type

Plant, Animal & Protists (microorganisms)

Unicellular organism- organism of one cell

Multi-cellular organism- organism of many cells Prokaryote- cells that lack specific nucleus

Eukaryote- cells with well-defined nucleus

Classification of Protists

• Prokaryotes

• Eukaryotes

Classification of Living Systems8

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Classification of living system9

Biocatalyst: cells and enzymes

10-20 µm 1-5 µm

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Comparision11

Components of Cell

Proteins

Nucleic Acid

Lipids & Membranes

Carbohydrates Cell wall

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Chemical composition of cells

Elemental composition C, H, N, P, O, S, Ash

Molecular analysis  Water, Protein, Nucleic Acid, Lipid, Population in culture, dry

 wt in culture

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Cells are building blocks:Tissue- collection ofcells with specificfunctionsOrgans- collections of

tissues with specificfunctionsOrgan systems-collections of organs with specific functions

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The Hierarchy of biologicalstructure

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Biochemical Components17

Proteins

Carbohydrates

lipids & Fats

nucleic acid

Enzymes