institute of physical chemistry polish academy of...

Institute of Physical Chemistry Polish Academy of Sciences

address: M. Kasprzaka 44/52, PL-01-224 Warsaw, Poland website: http://ichf.edu.pl phone: 48 22 3433109 fax: 48 22 3433333

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NanOtechnology, Biomaterials and aLternative Energy Source for ERA integration

FP7–REGPOT–CT–2011–285949-NOBLESSE

EUROPEAN

UNION

Warsaw, 29 October 2013

Report No. 8

NOBLESSE Project

July – September 2013

WP3. Chemo- and biosensors – molecular imprinting

Task Leader – Prof. Włodzimierz Kutner

Task Vice-Leader – Iwona Flis-Kabulska, D.Sc.

Task 3.1 Transfer of knowledge and experience through twinning and networking

During the period of 22nd

till 30 July, 2013, Dr. Agnieszka Pietrzyk-Le visited the Department of

Chemistry of the University of Milan, Milan (Italy), a partner of the NOBLESSE Project, Work

Package WP3 „Chemo- and Biosensors – molecular imprinting”. The purpose of the trip to Milan, was

to discuss possibilities of using most appropriate cross-linking monomers for molecular imprinting of

oligonucleotides. During her stay in Milan, Dr A. Pietrzyk-Le had a chance to meet several

researchers of this Department, and Prof. Patrizia R. Mussini, Prof. Emauela Licandro, Prof. Stefano

Maiorana, and Prof. Francesco Sannicolò among them. For Dr. A. Pietrzyk-Le, the discussions with

researchers of this Department provided valuable information on the latest trends in the field of

conducting polymers and their monomers.

Dr. Agnieszka Pietrzyk-Le giving the lecture

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The research achievements of Dr. A. Pietrzyk-Le were appreciated by a number of researchers of

this Department and, therefore, she was invited to give a lecture. Accordingly, on July 26, 2013 Dr.

A. Pietrzyk-Le gave a lecture at a departmental seminar, entitled: "Molecularly imprinted polymers

for development of chemical sensors for the biological target determination".

After the lecture, the discussion was focused on preparation and characterization of molecularly

imprinted polymers (MIPs), as well as calculation of the stability constants of affinity complexes of

MIPs and analytes.

Within discussions, novel thiophene-based oligomer T8 was chosen as a cross-linking monomer

for the collaborative future research. This is an asymmetric multithiophene-based monomer, which

can act as an effective 3 D electropolymerisation promoter.

The Report of the visit to the University of Milan, Milan, Italy is enclosed (Enclosure No. 1).

During the period of 22nd

till 28th September, 2013, Prof. Tadeusz Zakroczymski, Dr. Iwona Flis-

Kabulska and Dr. Arkadiusz Gajek visited Dr. Yong Sun, the researcher employed by the School of

Engineering, Media, and Sustainable Development of the Faculty of Technology of De Montfort

University (DMFU), Leicester (United Kingdom). Dr. Yong Sun is a Partner in the NOBLESSE

Project, Work Package WP3, “Chemo- and Biosensors - molecular imprinting”.

The visit in DMFU was related to the Polish

project funded by the National Science Centre,

“Surface modification with carbon introduced by

plasma treatment and deposition from solution for

improvement of electrocatalytic properties of cathodes

for production of hydrogen” realized jointly by IPC

PAS and DMFU.

Dr. Y. Sun, Dr. A. Gajek, Dr. I. Flis-Kabulska and Prof. T.

Zakroczymski in front of the School of Engineering, Media

and Sustainable Development, Faculty of Technology,

DMFU.

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The visit enabled our group to get acquainted with the works carried out at the Faculty of

Technology, DMFU. Dr. Y. Sun gave us a tour over several laboratories of the DMFU campus.

Our delegates participated in the seminar of the School of Engineering, Media and Sustainable

Development. Prof. T. Zakroczymski presented our Institute including its historical and present

research activities, with particular reference to the Department of Electrochemistry, Corrosion and

Applied Surface Science of IPC PAS. Dr. I. Flis-Kabulska and Dr. A. Gajek gave lectures based on the

recent results obtained in our Corrosion and Hydrogen Group.

Lectures presented by a group of Doctors and PhD Students enabled us to become more familiar

with the variety of research topics studied at the Department, which we visited.

A large part of the visit was devoted to discussing our joint project with Dr. Yong Sun. Our

discussions allowed establishing conditions of preparation of the studied materials, as well as the

methods for their characterisation. We agreed on how to realize the project and on assigning the

research tasks.

The Report of the visit to the De Montfort University in Leicester, UK is enclosed (Enclosure No.

2).

Task 3.2 Recruitment of one experienced researcher

On 1 August 2012, Dr. Piotr Pięta filled the position of an Experienced Researcher as the Assistant

Professor (“adiunkt” in Polish) in the Laboratory of Molecular Films (LMF) of the IPC PAS headed

by Prof. Włodzimierz Kutner.

According to the earlier provisions, the employment of Dr. P. Pięta was extended for the next 12

months – from 1 August 2013 till 31 July 2014.

Between July and September 2013, Dr. P. Pięta published, as a co-author, the following articles:

1. P. Pieta, I. Obraztsov, F. D’Souza, W. Kutner, “Composites of Conducting Polymers and Various

Carbon Nanostructures for Electrochemical Supercapacitors”, Journal of Solid State Science and

Technology, 2 (10) (2013) M3120-M3134.

2. T.-P. Huynh, P. Pieta, F. D'Souza, W. Kutner, “Molecularly imprinted polymer for recognition of 5-

fluorouracil by RNA-type nucleobase pairing”, Analytical Chemistry 85 (17) (2013) 8304-8312.

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3. M. Sosnowska, P. Pieta, P.S. Sharma, R. Chitta, C.B. Kc, V. Bandi, F. D'Souza, W. Kutner,

“Piezomicrogravimetric and impedimetric oligonucleotide biosensors using conducting polymers of

biotinylated bis(2,2′-bithien-5-yl)methane as recognition units”, Analytical Chemistry 85 (15) (2013)

7454-7461.

Dr. P. Pięta as the Project Leader of two proposals, one funded by the Foundation for Polish

Science, FNP, (“Change of nanomechanical properties of phospholipid bilayers in the presence of

cholesterol and antimicrobial peptides”), and the other by the National Science Centre, NCN,

(“Nanomechanical, visco-elastic, and electrochemical properties of conducting composites for

supercapacitors”) is responsible for organizing a modern laboratory for a new research group.

The renovated lately laboratory has been equipped in new instrumentation, as follows:

1. A system for chemical synthesis, Büchi AG.

2. A scanner and bipotentiostat for electrochemical experiments to be conducted with our AFM/STM

microscope, Bruker/Vecoo.

3. A multichannel potentiostat/galvanostat, BioLogic.

Task 3.3 Formation of independent research groups by young leader researchers

For recruitment of new employees to the research group of Dr. P. Pięta – one PhD student and two

master students, Calls for Applications were posted on 5 August 2013 and 17 October 2013.

The following persons have been employed:

1. Kamila Łępicka (PhD student) involved in project financed by NCN (Sonata 4).

2. Marta Majewska (master student) involved in project financed by FNP (Homing Plus)

3. Letycja Barzyk (master student) involved in project financed by FNP (Homing Plus)

Besides a new research group of Dr. P. Pięta, establishing of another two new research groups is

envisioned. One group will be organized by Dr. Agnieszka Pietrzyk-Le based on her research project,

entitled: “Selective determination of chosen oligonucleotides by synthetic polymer systems of

molecular recognition” funded by the Foundation for Polish Science within POMOST Programme

(Parent/Bridge Programme). A Call for Applications for the position within the group was posted on

26 April 2013. The position was filled by a PhD student, Ms. Katarzyna Bartołd. She passed a job

interview on 7 October 2013 and was admitted for the International Doctoral Studies at IPC PAS.

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The other group will be organized by Dr. Krzysztof Noworyta based on his research project,

entitled: “Chemosensors for selective determination of renal dysfunction biomarkers using

molecularly imprinted polymers, deposited by electropolymerization, as recognition layers”, funded

by the National Science Centre. The position within the group was filled by a PhD student, Valeri

Malyshev. He passed the job interview on 7 October 2013 and was admitted for the International

Doctoral Studies at IPC PAS.

Task 3.4 Facilitation of participation of the LMF and CHG researchers of IPC PAS in

international scientific conferences

The NOBLESSE Project enabled us for participation in the following international scientific

meetings.

1. IUPAC 10th

International Conference on Advanced Polymers via Macromolecular

Engineering, 17 – 24 August 2013, Durham, UK

Dr. Krzysztof Noworyta actively participated in the IUPAC 10th

International Conference on

Advanced Polymers via Macromolecular Engineering held at the Durham University, Durham, UK,

August 18 to 22, 2013.

The Conference gathered ~ 400 participants from all over the world working in the field of polymer

and materials sciences. Among the invited speakers, there were such eminent scientists as prof. K.

Matyjaszewski from the Carnegie Mellon University, prof. M. Sawamoto from the Kyoto University,

and prof. K. Landfester from the Max Planck Institute for Polymer Research.

During this conference, Dr. K. Noworyta presented the poster, entitled: “Application of the

Electrochemically Deposited Conducting Polymers as Molecularly Imprinted Recognition Units for

Creatinine Chemosensors” by P. Bocian, K.R. Noworyta, J.W. Sobczak, Ch.B. KC, F. D’Souza and

W. Kutner.

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Dr. K. Noworyta presenting the poster.

Participation in this conference allowed Dr. K. Noworyta to present results of the work, performed

in the Laboratory of Molecular Films at the Institute of Physical Chemistry PAS, to the world-class

specialists in the field of polymer chemistry and physics. Moreover, participation in this event

permitted Dr. K. Noworyta to learn modern trends in methods of polymer synthesis, processing, and

their practical applications as well as new techniques of characterization of these materials.

The Report of the participation in the IUPAC 10th

International Conference on Advanced Polymers

via Macromolecular Engineering is enclosed (Enclosure No. 3).

2. The 64th

Annual Meeting of the International Society of Electrochemistry, 8-13 September

2013, Santiago de Queretaro, Mexico

The 64th

Annual Meeting of the International Society of Electrochemistry, entitled

“Electrochemistry for a New Era” was held on 8 through 13 September 2013 in Santiago de

Queretaro, Mexico. The conference brought together ~1000 scientists from around the world

(including ~40 from Poland). They presented the most recent achievements of electrochemistry and

challenges it faces now. Five distinguished electrochemists delivered plenary lectures reviewing and

giving the newest information on the intriguing themes of electrochemistry including

photoelectrochemical cells (M. Grätzel), molecular electronics (R. L. McCreery), ionic liquids in

electrochemical devices (D. R. MacFarlane), interaction of oxygen radicals with the electrode surface

(F. Schulz) and new advanced materials for fuel cells (M. Watanabe).

Topics discussed at the Meeting included a wide range of issues, including among others

Bioelectrochemistry and Biosensors applied to Medicine, Molecular Electrochemistry: from Single

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Molecules to Conducting Polymers, Surface Treatment for Aerospace Applications and Corrosion

Protection, Industrial Electrolysis, Electrochemical Energy Conversion and Storage.

The European Project NOBLESSE was represented at the Meeting by four researchers of the

Work Package WP3 “Chemo- and Biosensors- molecular imprinting”. They delivered the following

presentations (names of the presenting authors are underlined):

1. W. Kutner, Tan-Phat Huynh, M. Sosnowska, C.

Bikram K.C., V. Nesterov, J. W. Sobczak, F. D'Souza,

A General Protocol of Designing and Fabricating

Thin Films of Conducting Molecularly Imprinted

Polymers for Application to Selective Sensing.

(Lecture; Symposium 3: New Concepts for Designing

Bioelectrochemical Interfaces)

2. K. R. Noworyta, W. Kutner, C. A. Wijesinghe, F.

D’Souza, Electrochemical and

Piezomicrogravimetric Detection of Selected

Alkaloids using a Zinc Porphyrin Polymer with

Controlled Porosity. (Lecture; Symposium 6:

Conducting Polymers, Inorganic Materials and

their Hybrids for Electrocatalysis and

Photoelectrochemical Energy Conversion)

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3. M. Dąbrowski, P. S. Sharma, Z. Wróbel, W. Kutner, Electrochemically-induced mesoporous

molecularly imprinted organosilica film, deposited as recognition unit of chiral chemosensor for

selective detection and determination of L-arabitol.(Poster; Symposium 2: Sensing in Living Systems)

The poster has been awarded with the Best Poster Award presented at the 64th ISE Meeting.

4. I. Flis-Kabulska, J. Flis, T. Zakroczymski, Antimony Microelectrode for Facile Measurements of

the Near-Surface pH. Identification of Electrode Processes Involving Protons. (Poster; Symposium

5: Corrosion Processes at the Nanoscale; Surface

Treatments)

5. J. Flis, I. Flis-Kabulska, Determination of

Hydrogen Absorption in Iron Cathodes for Alkaline

Water Electrolysis by Anodic Discharging and

Electrochemical Permeation Technique. (Poster;

Symposium 12: Tradition to Modernity: Challenges at

the Electrochemical Interface; Electrocatalysis)

Vivid discussions with the authors of the lectures and of the above-mentioned posters indicated

that there was a significant interest in the research results presented by the researchers from the IPS

PAS.

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The active participation of the researchers from the IPC PAS

in the 64th Annual Meeting of ISE was extremely useful because it

gave us this opportunity of both getting acquainted with most

recent achievements in selected fields of electrochemistry and to

confront our own research achievements with opinions of the

world electrochemistry community.

The 64th Annual Meeting of ISE gave us also opportunity to

promote the Institute of Physical Chemistry PAS. Books and

leaflets presenting IPC PAS captured participants’ attention and

were quickly distributed.

The Report of the participation in the 64th Annual Meeting of the International Society of

Electrochemistry is enclosed (Enclosure No. 4).

3. The 6th

Kurt Schwabe Symposium, 16-19 September, 2013, Cracow, Poland

The 6th Kurt Schwabe Symposium was organized in Cracow (Poland) on 16 through 19

September, 2013, as continuation of the Kurt Schwabe Symposia organized previously in various

towns of Europe. These symposia bring together researchers from all over the world who focus their

scientific interests on new materials and new electrochemical techniques in electrochemical

technologies, corrosion science, and corrosion engineering. There were 6 plenary lectures and 9

invited lectures delivered. Moreover, 38 communications and 22 posters were presented.

Three researchers of the European Project NOBLESSE, Work Package WP3 “Chemo- and

Biosensors- molecular imprinting”, of the Corrosion and Hydrogen Group actively participated in the

Meeting. They delivered the following presentations (names of the presenting authors are underlined):

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1. T. Zakroczymski, “Stress corrosion cracking

and hydrogen embrittlement” (invited lecture;

Session 8 – Hydrogen in metals)

2. A. Gajek, I. Flis-Kabulska, T. Zakroczymski,

Cheng-Hsien Yang, Jeng-Kuei Chang, Wen-Ta

Tsai, “Diffusion of hydrogen in magnesium

studied by the electrochemical permeation

metod” (Lecture; Session 8 – Hydrogen in

metals)

3. I. Flis-Kabulska, J. Flis, T. Zakroczymski,

“Hydrogen permeation measurements for

evaluation of absorbed hydrogen and of

surface changes on iron cathodes during

alkaline water electrolysis”(Lecture; Session 8

– Hydrogen in metals)

4. I. Flis-Kabulska, J. Flis, Y. Sun, T. Zakroczymski, “Measurement of pH at the electrode

surface for better understanding the corrosion behaviour of low-temperature plasma

nitrided stainless steel” (Lecture; Session 10 – Corrosion resistant alloys)

Organizing of small international conferences, which bring together less than ~100 participants,

gives the unique opportunity to meet world renowned researchers in the area of our interest, to listen to

their lectures and to discuss with them.

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The Report of the participation in the 6th Kurt Schwabe Symposium is enclosed (Enclosure No. 5).

For the WP3 researchers, participation in the Scientific Meetings allowed to present their results to

the international electrochemistry community. The scientists of IPC PAS could discuss their research

with a large group of electrochemists that allowed them to look at their own research from different

perspectives. The Meetings gave us this opportunity to learn new research topics and to make new

acquaintances with foreign researchers of similar or complementary scientific interest for future

collaborative twinning.

Task 3.5 Organization of the 6th

International Workshop on Surface Modification for

Chemical and Biochemical Sensing (SMCBS’2013)

The SMCBS’2013 Workshop will be organized in Lochow (Pol. Łochów) near Warsaw on 8 till

12 November 2013. Information on the SMCBS’2013 Workshop is available on the website at

http://smcbs2013.pl.

The final List of Participants includes 114 scientists from all over the world. The total number of

submitted abstracts was 104. Oral presentations will include 19 keynote lectures, 14 tutorial lectures,

and 13 short communications. Moreover, fifty eight posters will be presented.

The Book of Abstracts with an ISBN number assigned will be published and distributed to all

participants at the Workshop.

Miscellaneous issues:

Articles published with acknowledgement to NOBLESSE Project.

1. P. Pieta, I. Obraztsov, F. D’Souza, W. Kutner, “Composites of Conducting Polymers and

Various Carbon Nanostructures for Electrochemical Supercapacitors”, Journal of Solid State Science

and Technology, 2 (10) (2013) M3120-M3134.

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2. T.-P. Huynh, P. Pieta, F. D'Souza, W. Kutner, “Molecularly imprinted polymer for recognition

of 5-fluorouracil by RNA-type nucleobase pairing”, Analytical Chemistry 85 (17) (2013) 8304-8312.

3. M. Sosnowska, P. Pieta, P.S. Sharma, R. Chitta, C.B. Kc, V. Bandi, F. D'Souza, W. Kutner,

“Piezomicrogravimetric and impedimetric oligonucleotide biosensors using conducting polymers of

biotinylated bis(2,2′-bithien-5-yl)methane as recognition units”, Analytical Chemistry 85 (15) (2013)

7454-7461.

4. I. Flis-Kabulska, Y. Sun, J. Flis “Monitoring the near-surface pH to probe the role of nitrogen in

corrosion behaviour of low-temperature plasma nitrided 316L stainless steel”, Electrochimica Acta

104 (2013) 208– 215.

5. I. Flis-Kabulska, Y. Sun, J. Flis “ Corrosion Characteristics of Low Temperature Plasma

Carburised and Nitrocarburised 316L Stainless Steel in Sulphate-Chloride Solution” Zeitschrift Fur

Physikalische Chemie - International Journal Of Research In Physical Chemistry & Chemical

Physics 227 (8) (2013) 1105-1119.

6. P. S. Sharma, M. Dabrowski, F. D’Souza, W. Kutner “Surface development of

molecularly imprinted polymer films to enhance sensing signals“ TrAC Trends in Analytical

Chemistry 51 (2013) 146-157.

7. Tan-Phat Huynh, Chandra Bikram K.C., Wojciech Lisowski, Francis D'Souza,

Wlodzimierz Kutner “Molecularly imprinted polymer of bis(2,2′-bithienyl)methanes for

selective determination of adrenaline“ Bioelectrochemistry 93 (2013) 37-45.

8. Piotr Pieta, Ievgen Obraztsov, Janusz W. Sobczak, Olga Chernyayeva, Sushanta K.

Das, Francis D’Souza, Wlodzimierz Kutner “A Versatile Material for a Symmetrical Electric

Energy Storage Device: A Composite of the Polymer of the Ferrocene Adduct of C60 and

Single-Wall Carbon Nanotubes Exhibiting Redox Conductivity At Both Positive and

Negative Potentials“ Journal of Physical Chemistry C 2013, 117, 1995-2007.

Enclosures

1. The Report of the visit to the University of Milan, Milan, Italy is enclosed

(APietrzykLe_WP3_staż.doc).

2. The Report of the visit to the De Montfort University in Leicester, UK (Report_TZ AG IFK.doc)

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3. The Report of the participation in the IUPAC 10th

International Conference on Advanced Polymers

via Macromolecular Engineering (KNoworyta_APME_2013_sprawozdanie_1.doc)

4. The Report of the participation in the 64th Annual Meeting of the International Society of

Electrochemistry (WP3 Meksyk ISE_report_2013.doc)

5. The Report of the participation in the The 6th Kurt Schwabe Symposium (KSS

Krakow_report_2013 IFKphoto.doc)

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