inhibitors of electron transport chain & oxidative phosphorylation ppt biochemistry
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INHIBITORS OF ETC & OXIDATIVE PHOSPHORYLATION
Dr Vijay Marakala M.D
Assistant professorBiochemistry
SIMS&RC
SHUTTLE SYSTEMS SUBSTRATE LEVEL PHOSPHORYLATION
INHIBITORS
Inhibitors of the ETC proper
Inhibitors of oxidative
phosphorylation [FoF1ATPase]
Uncouplers of oxidative
phosphorylation
INHIBITORS OF ETC PROPER
INHIBITORS OF ETC PROPER
Complex-IComplex-IIIComplex-IV
Blocking ETC Proton pumping
ATP synthesisOxygen uptake
Results in block
Inhibitors of first site or complex-I
NAD FMN Fe-S CoQ Cyt.b Fe-S Cyt.c1Cyt.c Cyt.aa3 O2
Antibiotic
Barbiturates
InseCticide
• Piericidin
• Amobarbitol
• Rotenone
Inhibitors of second site or complex-III
NAD FMN Fe-S CoQ Cyt.b Fe-S Cyt.c1 Cyt.c Cyt.aa3 O2
Dimercaprol
Antimycin A
Inhibitors of second site or complex-IV
NAD FMN Fe-S CoQ Cyt.b Fe-S Cyt.c1 Cyt.c Cyt.aa3 O2
Cyanide
CO
H2S
Inhibitors of oxidative phosphorylation
Block Phosphorylation directly by inhibiting FoF1ATPase
Oligomycin Dicyclohexyl carbodiimide
[DCCD]
Uncouplers of oxidative phosphorylation
Uncouplers of oxidative phosphorylation
2,4 Dinitrophenol[DNP]
Dicumarol
Chlorocarbonylcyanide phenylhydrazone[CCCP]
Salicylate
Thermogenin
THERMOGENIN a natural
uncouplerH+
SHUTTLE SYSTEM FOR
EXTRAMITOCHONDRIAL NADH
Transport of reducing equivalents from cytosol
Inner mitochondrial membrane is not permeable to cytoplasmic NADH
Shuttle system for oxidation of Extramitochondrial NADH
Malate – aspartate shuttle
Glycerol phosphate shuttle
Malate – aspartate shuttle
Glycerol phosphate shuttle
36 or 38
Substrate level phosphorylation
Substrate level phosphorylation
ENERGY CURRENCY OF CELL
ATP
ADP
ADP+Pi
AMP+Pi
AMP Adenosine+Pi
G0= -7.3Kcal/mole
G0= -7.3Kcal/mole
G0= 3.4Kcal/mole
There are three major sources of taking part in energy
conservation or energy capture:
Oxidative phosphorylation
Glycolysis
TCA cycle
Role of ATP/ADP cycle in transfer of high-energy phosphate
There will be a test on Wednesday
[9am-10am] MQ-1 [10 marks]
S/N-2 [5X2=10 marks]