Brief Reports

Vox Sang. 25: 540-542 (1973)

Independence of the Colton and Yt Blood Group Systems'

MARION LEWIS, HIROKO KAITA, ELOISE R. GIBLETT and A. G. STEINBERG

Rh Laboratory, Winnipeg. King County Central Blood Bank, Seattle, Wash., and Depart- ment of Biology, Case. Western Reserve University, Cleveland, Ohio

Absrruct. Record is made of a family which provides evidence of the independent segregation of: the Colton and Yt blood group antigens; Colton and acid phosphatase red cell enzyme; Yt and phosphoglucomutase red cell enzyme.

The Colton and Yt blood group systems, each to date comprising a pair of antigens bearing antithetical relationships, have been shown to segregate independently of all established blood group systems excepting each other, Diego and, in the case of the former, Sm-Bu* [1-4].

The family here reported was selected for study because of heterozy- gosity of the propositus in both the Colton and the Yt systems. Blood samples from its members were tested for as many red cell antigens, red cell enzymes and serum groups as possible.

'New' information shown in the pedigree (fig. 1) indicates that genes that are not closely linked control the Colton (Co) and Yt blood group antigens, Co and acid phosphatase (AcPh) red cell enzyme, and Yt and phospho- glucomutase (PGMl) enzyme. The informative members of the family yield the following counts for z1 scores:

~~ ~~

Gen. I1 Gen. I11

C0:Yt 3:2 2: 1 Co:AcPh 2: 1 Yt : PGMl 6:3 2: 1

Work supported in part by an M.R.C. (Canada) grant.

Received: May, 29 1973; accepted: May 29, 1973.

LEW et al. 541

Co a+b+ Y t a+b- PGMl 1

Table I. Full groupings of Fox family

Co arb- V t a+b- a+bt a t b - a+b- a t b t a+b- a t b + a t b + PGMl 1 2-1 2-1 2-1 2-1 1 2-1

AcPh EA

ABO MNSs Rh Fy Lu Jk Co Yt Do Xg AcPh PGMl Hp Gm ab ab ab ab ab a a

a+b- atb+ a t b + 1 2-1 2-1 CA

1-2 A1 11-1 A1 11-3 0 11-4 0 11-5 0 11-6 A, 11-7 0 11-8 A1 11-9 0 11-10 B 11-11 A1 11-12 A1

111-1 0 111-2 B 111-3 0

~~~ ~ ~

NSNs R,r NsNs RIRl

NSNs rr MsNs Rlr MsNS rr NsNs R,r MsNs Rlr

MSNs RIRl MsNs Rlr MsNs rr

NSNS R1R1

MSNS RIR,

MSNS R1Rl MSMS R1R1 NSNS R1R1

++ -+ -+ ++ t + + + + - + + + t t + - -1- -+ ++ ++ ++ + + t 4 + + + + t + + ++ -+ ++ -+ t + - ++ 4 ++ ++ ++ - + t + + + + t t + + ++ -+ ++ ++ ++ + - -+ ++ ++ ++ ++ + - -+ -+ ++ t t - + -+ -+ ++ t ++ + + -+ -+ ++ -+ ++ + + - + + + + + t t - + -+ --f -+ +- t - + - t - + t + + t - +

A BA BA BA

1 2-1 3,5,13,14 1 2-2 3,5,13,14 2-1 2-2 3,5,13,14 2-1 2-2 1,2,3,5,13,14,21

BA BA BA BA CA BA BA A A A

2-1 2-1 2-1 2-1 1 2-1 2-1 2-1 1 2-1 2-1 2-1 2-1 2-1 2-1 2-2 1 2-2 1 2-2

1,2,3,5,13,14,21 1,2,3,5,13,14,21 3,5,13,14 3,5,13,14 1,2,3,5,13,14,21 1,2,3,5,13,14,21 3,5,13,14 1,2,3,5,13,14,2 1 1,2,3,5,13,14,21 1,2,3,5,13,14,21

Red cell antigens. All K- k+ Kp(a-b+), Vel+, Gy+, Ge+. LWt; members of generations I and I1 all Mg-, Vw-, Ny(a-), Wr(a-), Reid- Bu(a-), Sm+, Rd-, Di(a-), Bi(a-), Be(a-), Rieter-.

Red cell enzymes. All except 11-5 (who was not tested) LDH usual, 6PGD A, G6PD B, PepB 1, PepC 1, AK 1, ICD 1, Enol 1,2,3DPGM 1, PGAM 1, PHI 1, PGK 1, GOT 1, PFK 1, HK 1, PK 1, G3PD 1, Aldo 1, Hgb A, ADA 1, PepA 1 (11-1,3,7 and 11 weak). All GPT 1 except 11-10 who is GPT 2, and her offspring who are GPT 2-1.

Serum groups. All except 11-5 (who was not tested) Inv-1 and Tf C-C. All except 11-5 tested for Gm 1,2,3,5,6,13,14 and 21; only the positive results are listed in table I.

Fig. I. The Fox family.

542 LEWIS et al.

All of the results are set out in table I where confirmation can be found for Colton independence of ABO, MNSs, Lutheran, Duffy, Dombrock, PGMl, Gm and Hp groups and sex, and, for Yt, independence of MNSs, Rh, Lutheran, and Grn groups, and sex.

Acknowledgements

We are much indebted for gifts of antisera to Mrs. JANE SWANSON (anti-Co*), Dr. B. P. L. MOORE (anti-Coa and -Yta) and to Dr. H. A. WURZEL (anti-Ytb). We are also most grateful to the family here reported for their willing cooperation.

References

1 EAMN, B. R.; MORTON, J. A.; PICKLES, M. M., and W m , K. E.: A new antibody, anti-Yta, characterizing a blood-group antigen of high incidence. Brit. J . Haemat. 2:

2 GILES, C. M.; METAXAS-BUHLER, M.; ROMANSKI, Y., and METAXAS, M. N.: Studies on the Yt blood group system. Vox Sang. 13: 171-180 (1967).

3 HEISTO, H. ; HART, M. VAN DER; MADSEN, G . ; MOES, M.; NOADES, J.; PICKLES, M. M.; RACE, R. R.; SANGER, R., and SWANSON J. : Three examples of a new red cell antibody, anti-Coa. Vox Sang. 12: 18-24 (1967).

4 LEWIS, M.; KAITA, H. ; ANDERSON, C., and CHOW, B. : Independence of Colton blood group. Transfusion 11: 223-224 (1971).

333-341 (1956).

Request reprints from: Miss MARION LEWIS, Rh Laboratory, 735 Notre Dame Avenue, Winnipeg 3 (Canada)