in vivo multiple color lymphatic imaging using ... · in vivo multiple color lymphatic imaging...
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Supplementary Material (ESI) for Soft Matter This journal is (c) The Royal Society of Chemistry 2009
Supplementary Data
In vivo Multiple Color Lymphatic Imaging Using
Upconverting Nanocrystals.
Hisataka Kobayashi1, Nobuyuki Kosaka1, Mikako Ogawa1,
Nicole Y. Morgan2, Paul D. Smith2, Christopher B. Murray3, Xingchen Ye3,
Josh Collins3, Ajith Kumar4, Howard Bell4, Peter L. Choyke1
1Molecular Imaging Program, Center for Cancer Research, National Cancer Institute,
NIH, Bethesda, MD 20892-1088
2National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD
20892
3University of Pennsylvania. Philadelphia, PA 19104
4Sunstone BioSciences, Inc., Philadelphia, PA 19104
*Corresponding author: Hisataka Kobayashi, MD, PhD
Molecular Imaging Program, NCI/NIH,
Bldg. 10, Room 1B40, MSC 1088, Bethesda, Maryland 20892-1088, USA
E-mail: [email protected]
Supplementary Material (ESI) for Soft Matter This journal is (c) The Royal Society of Chemistry 2009
Supplementary figure 1. Dynamic light scattering (DLS) measurements were performed on a
Delsa™ Nano C system (Beckman Coulter). Hexane solutions of nanocrystals with concentration of
about 0.2mg/ml were used. DLS data for the as-synthesized nanocrystals confirm the TEM results,
showing an average particle size of 20.6-nm, seen in figure 1.
DLS data on the as-injected, functionalized nanocrystal suspension is not currently available,
however based on previously published works1,2, we surmise a surface PEG coating of ~15-20-nm in
thickness, yielding an approximate final particle size of 50-60-nm in diameter.
In order to eliminate the aggregation or contamination, injected solution was safely passed through
0.22mm-filter (Millipore, Bedford, MA) immediately before injection with minimal loss, which
was measured by the spectral absorbance with a UV-Vis system (8453 Value UV-Visible Value System; Agilent Technologies, Santa Clara, CA). 1. Z. Liu, C. Davis, W. Cai, L. He, X. Chen, H. Dai. Proc. Natl. Acad. Science, 2008, 105, 1410-1415.
2. Torchilin, et.al. Eur J Pharm Biopharm. 2006, 62(3), 235-240.
Supplementary Material (ESI) for Soft Matter This journal is (c) The Royal Society of Chemistry 2009
Supplementary figure 2. (a) Room temperature upconversion emission spectra of
NaYF4:Yb/Er (green) and NaYF4:Yb/Tm (blue) nanocrystals under 980nm excitation.
(b) Powder X-ray diffraction pattern of the NaYF4:Yb/Er nanocrystals.
Supplementary Material (ESI) for Soft Matter This journal is (c) The Royal Society of Chemistry 2009
Supplementary figure 3. Comparative single shot fluorescence lymphatic imaging obtained at 550 nm after injection of either the green upconverting nanocrystal (980 nm excitation) or the quantum dot 545 (468 nm excitation). No background is observed with the green upconverting nanocrystal. In contrast, due to the autofluorescence, the body structures are clearly shown in the image obtained with the quantum dot 545.