immunological assays - nvinvi.ddc.moph.go.th/download/ectd/module 1/8 mar/4... · 2016-03-10 ·...
TRANSCRIPT
![Page 1: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/1.jpg)
Immunological Assays1890 Behring and Kitasato --> Discovery of Abs
1975 Kohler and Milstein ---> hybridoma technique
1. Precipitation
2. Agglutination
3. Radioimmunoassay
4. Enzyme-linked Immunosorbent Assay (ELISA)
5. Flow Cytometry and Fluorescence
6. Immunochromatographic strip test (POCT)
Slides kindly provide by Prof. Chatchai Tayapiwatana, CMU
![Page 2: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/2.jpg)
![Page 3: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/3.jpg)
![Page 4: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/4.jpg)
Biosensors
![Page 5: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/5.jpg)
• NycoCard™• >18,000 instruments installed • 4 rapid tests :
• CRP (viral/bacterial infection)• HbA1c (diabetes-monitoring)• D-dimer (deep vein thrombosis)• Urine albumin (diabetes-
monitoring)• Distributed in 70 countries
• AFINION™ roll-out started• Advanced, fully automated multi-
assay instrument • Tailor-made for the US market
Optical sensor
![Page 6: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/6.jpg)
![Page 7: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/7.jpg)
AFINION™ Pipeline Short Term
MARKER PoC TEST VOLUMES
Million tests
END USER PRICE
US$
TOTAL PoC
MARKETUS$M
USE COMMENTS
HbA1cLaunched Europe and USA
20-25 6-8 120 Monitors glycaemic control in diabetic patients.
Market leader is Bayer with A-S (NycoCard™) no. 2.
CRPLaunched Europe
10 3-4 30 Distinguishes between viral and bacterial infection.
A-S NycoCard™market leader with Orion no. 2. Not introduced in US yet.
![Page 8: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/8.jpg)
Immunochromatographic strip test•First generation POCT•Qualitative
![Page 9: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/9.jpg)
![Page 10: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/10.jpg)
Optical density measurement
![Page 11: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/11.jpg)
Immunofluorescent Detector
![Page 12: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/12.jpg)
![Page 13: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/13.jpg)
![Page 14: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/14.jpg)
© Copyright 2009 by the American Association for Clinical Chemistry
![Page 15: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/15.jpg)
© Copyright 2009 by the American Association for Clinical Chemistry
![Page 16: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/16.jpg)
© Copyright 2009 by the American Association for Clinical Chemistry
![Page 17: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/17.jpg)
© Copyright 2009 by the American Association for Clinical Chemistry
![Page 18: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/18.jpg)
BioMEMS• Microelectromechanical system_MEMS• MEMS is being applied to biomedical applications and has
become a new field of research unto itself.• The interface between biology and microtechnology
encompasses many diverse areas and levels of biological complexity.
• Include: DNA arrays (genes), immmunoassays (proteins), miniaturized lab-on-a-chips (genes, proteins, and sensors), and finally the integration of live cells (bacterial and mammalian) with semiconductor materials and components.
![Page 19: Immunological Assays - NVInvi.ddc.moph.go.th/Download/eCTD/Module 1/8 Mar/4... · 2016-03-10 · Immunological Assays 1890 Behring and Kitasato --> Discovery of Abs 1975 Kohler and](https://reader033.vdocuments.us/reader033/viewer/2022041700/5e41615b1018264a631744c6/html5/thumbnails/19.jpg)
Microfluidics Applications