image analysis of daphnia
TRANSCRIPT
Daphnia is widely used in biotoxicity study The change of heart rate after in-taking
toxic substance is the measure of biotoxic-ity
Unfortunately, counting heat beat with naked eye is not easy because it jumps around continuously.
Fixing Daphnia on slide glass is also not a good solution because it causes big stress to the animal
Background
Non-invasive measurement. Fully automated Big data, presented as time series
curve on 2-dimensional area, is gener-ated that can be further analyzed
Complex pattern can be visualized
Advantages of image analysis of Daphnia motion
Experimental setup
Thin-layer cell made with cover glass(10mm x 10mm x 1.2mm
Camera with close-up lensComputer for image analy-sis
Daphnia trapped in a air bubble
Image analysis
Original image
conversion Track center of gravity
1. Download ‘Roborealm’ software2. Make a script (see right)3. Run the script, COG and time is saved au-tomatically
Made of cover glass Daphnia lives for a long time Thickness can be adjusted Two cover glasses are sandwiched with
molten paraffin Cheap and easy
Mini glass aquarium
Grow Daphnia in a aquarium Feed Chlorella Make mini aquarium with cover glass Inject a Daphnia into it. Seal the opening with paraffin Put it on camera and run RoboRealm Run the script and collect data Feed different substance to Daphnia Make a software that display the COG, time data as a
2D curve
Method
Result 1
A spike caused by error image analysis software
A Daphnia is moving along the edge.
After 48 hours, mother Daphnia is died. More than ten baby daphnia are hatched and gathered at this area
A Daphnia is left in the cell for 24 hours
Image analysis may provide more useful in-formation of biotoxicity
Motion pattern could be analyzed if neces-sary
Conclusion
1. RoboRealm : http://www.roborealm.com/tutorials.php
2. Image processing software – provided by Biolocs Co. Ltd. Korea
Reference