daphnia toxicology experiment
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Daphnia Toxicology Experiment. Bridges 2014. Daphnia. Freshwater planktonic crustaceans C yclic parthenogenesis Favorable conditions: Asexual reproduction of females Unfavorable conditions: Sexual reproduction Reproductive maturity: 3-5 days Fine mesh feeders Eat bacteria and algae - PowerPoint PPT PresentationTRANSCRIPT
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Daphnia Toxicology Experiment
Bridges 2014
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DaphniaFreshwater planktonic crustaceansCyclic parthenogenesis
Favorable conditions: Asexual reproduction of femalesUnfavorable conditions: Sexual reproduction
Reproductive maturity: 3-5 daysFine mesh feeders
Eat bacteria and algaeSwim with an erratic, jerking motion then hang
motionless in the waterMany predators, including fish, shrimp and copepods
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ToxicityThe degree to which a substance can damage
an organism or the environmentToxicity is dose dependent
Too much water can be deadlyJust a little snake venom can be deadly
Many household products are toxicThis is an issue when they enter the
environment or our drinking water!
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Examples Household ToxinsDrain cleaners Oven cleanersLaundry detergentsFloor or furniture polishPaints PesticidesDisinfectantsCleaning ProductsMany others…
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Testing Toxicity with DaphniaPick a substance that you think will be toxic
to DaphniaCreate serial dilution of substance
Think carefully about what dilutions to useShould include:
Positive control (will kill) Negative control (won’t kill)
Transfer live Daphnia to plate containing dilution of potentially toxic substance
Score dead/live Daphnia in each treatment daily
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