hoefer catalog online

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The Companies of Harvard Bioscience.............iiGlobal Distribution.............................................ivNew Products.....................................................viHoefer Product Range....................................viii

SE 600 Chroma................................................3SE 600 and SE 660.........................................4SE 640 Wide Mini............................................5SE 400 and SE 410 Sturdier..........................6Gel Casters for Standard Format Gels............7Accessories & Spare Parts............................8-10

Protein Electrophoresis

Introduction

Standard Vertical Systems...........................1-2

Small Format Vertical Systems...............11-12SE 300 miniVE.........................................13-14Mighty Small II, SE 250 and SE 260.......15-16Gel Casters for Small Format Gels..................17Accessories.......................................................18

SG Series.......................................................20

HE 33 Mini....................................................22HE 99X Max...................................................23Accessories & Spare Parts............................24

Nucleic Acid ElectrophoresisSubmarine Agarose Units.......................21-24

TE 70X and TE 70XP..................................27TE 77X and TE 77XP..................................28Membranes................................................34

Protein BlottingBlotting.................................................25-30

TE 22 Mighty Small....................................29TE 62 and TE 42......................................30Membranes................................................34

Tank Blotters...........................................29-30

ImmunoBlot and ImmunoBlot XL................32Slot Blot and DecaProbe..............................33Membranes.................................................34

ImmunoblottingImmunoblotting...................................31-33

FiltrationFiltration Manifold.........................................35

Electrophoresis ReagentsGel Casting......................................................39Sample Preparation and Loading....................40Buffer and Buffer Preparation..........................41Gel Staining......................................................42

Imaging

ULTima 10si, 16si, 16si Plus and Pro........45-46Optix Illumination Module.............................47TotalLab Analysis Software.........................47-48

ULTima Imaging Platforms.....................43-48

UV Transilluminators.....................................49

QuantitationFluorometer for DNA/RNA Quantitation.........50

Detection

HB-1000 Hybridization Oven......................51-52UVC 5000 UV Crosslinker..........................51-52SE 1200 Easy Breeze Gel Dryer....................53GD 2000 Gel Dryer.........................................54

Detection..............................................51-54

Radiation Safety ProductsBeta and Gamma Radiation Protection......55-56

UV/ Visible SpectrophotometersBiochrom Libra Series....................................57Biochrom WPA Series....................................58

Amino Acid AnalyzerBiochrom 30 Series...................................59-60

Sample PreparationHarvard Apparatus Products......................61-62

General InformationTechnical Appendix..........................................63Online Ordering and Support........................64Contact Information........................................65Terms and Conditions.......................................66Bibliography........................................67-68

Custom ProductsCustom Manufacturing.....................................69

SE 100.............................................................19

Gradient Makers

Plate Washer & Storage Kit


Power SuppliesEV 265 Consort.............................................37Hoefer PS 2A200..............................................38

Circulation BathRefrigerated & Heated Circulation Bath........36

Semi-Dry Blotters...................................27-28

High Quality Electrophoresis,Blotting, Quantitation and DetectionSystems and More.

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HARVARD BIOSCIENCEAreas of Concentration

MolecularBiology

CellBiology

TissueStudies

IsolatedOrgan

WholeAnimal

Company ProductsHa

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Pumps, Ventilators, Anesthesia andSample Preparation Products PlusThousands More.

Physiology Recorders, Amplifiers,Transducers, Surgical Tables &Homeothermic Blankets

The Technology Leader inElectroporation and Electrofusion.

Equilibrium Dialysis, Dialysis, SPEand Sample Preparation Products

Clark ElectromedicalWorld Standard Capillary Glass

Hugo Sachs ElektronikIsolated Organ & Tissue Systems,Data Acquisition

International Market SupplyAnesthesia & Animal Behavior Products

Micro-Injectors, Micro-Incubators,Brain Slice Chambers andIontophoretic Pumps

Diffusion/Ussing Chambers,Substance Transport Studies

Patch Clamp Amplifiers & Systems,Micro-Perfusion Chambers, Cell ImagingMicro-Incubators, Ussing Systems

Spectrophotometers &Amino Acid Analyzers

Microplate Readers,Washers & Dispensers

Full Range of Electrophoresis &Radiation Protection Products

Walden Precision ApparatuspH Meters, Conductivity Meters,Colorimeters, Oxygen Meters andSpectrophotometers

Genomic & Proteomic ResearchTools for Complete DrugDiscovery Solutions

Products for Large BoreFlow Cytometry

Products for High ContentImaging & Analysis

Family of Companies

United States

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New BioresearchProducts Catalog1100 page catalog now available.Call for your FREE copy:

800-272-2775or +1-508-893-8999

Contact Information

Harvard Apparatus84 October Hill RoadHolliston, Massachusetts 01746Phone: 1-508-893-8999Toll Free: 1-800-272-2775 (USA & Canada)Fax: 1-508-429-5732Email: [email protected]: harvardapparatus.comA wide variety of research products includingindustry leading syringe pumps, ventilators,microinjectors, microincubators, temperaturecontrollers, capillary glass, advanced samplepreparation products and many, many more.

Union Biometrica, Inc.US Headquarters84 October Hill RoadHolliston, Massachusetts 01746Phone: 1-508-893-3115Fax: 1-508-893-8044Email: [email protected]: unionbio.comProducts for large bore flow cytometry.

Warner Instruments, LLC.1125 Dixwell AvenueHamden, Connecticut 06514Phone: 1-203-776-0664Toll Free: 1-800-599-4203 (USA & Canada)Fax: 1-203-776-1278Email: [email protected]: warneronline.comThe industry leader for electrophysiology andcell biology products including clamp amplifiers& systems, micro-perfusion and imagingchambers, Ussing systems and micro-incubators.

Genomic Solutions, Inc.4355 Varsity Drive, Suite EAnn Arbor, Michigan 48108Phone: 1-734-975-4800Toll Free: 1-877-436-6642 (USA & Canada)Fax: 1-734-975-4809Email: [email protected]: genomicsolutions.comGenomic and proteomic research tools forcomplete drug discovery solutions.

United KingdomHarvard Apparatus, Ltd.Fircroft Way, EdenbridgeKent TN8 6HE, UNITED KINGDOMPhone: (44) 1732-864-001Fax: (44) 1732-863-356Email: [email protected]: harvardapparatus.co.ukHarvard Apparatus, Ltd. manufactures a widerange of physiology products includingrecorders, amplifiers, transducers, surgicalequipment and the new IMS anesthesiaproduct line.

Biochrom, Ltd.Cambridge Science Park, Unit 22Milton RoadCambridge CB4 0FJ, UNITED KINGDOMPhone: (44) 1223-423723Fax: (44) 1223-420164Email: [email protected] manufactures a full line ofspectrophotometers and amino acid analyzers.

Scie-Plas, LimitedUnit 3 Gainsborough Trading EstateOld Road, SouthamWarwickshire CV47 1HP,UNITED KINGDOMPhone: (44) 1926-814093Fax: (44) 1926-813975Email: [email protected] manufactures high qualityelectrophoresis and radiation protectionproducts.

Walden Precision Apparatus, Ltd.Cambridge Science Park, Unit 22Milton RoadCambridge CB4 0FJ, UNITED KINGDOMPhone: (44) 1223-423723Fax: (44) 1223-420164Email: [email protected] Precision Apparatus brings a widerange of products to our offering including pHmeters, conductivity meters, colorimeters,oxygen meters and spectrophotometers.

AustriaASYS Hitech GmbHNordstrasse 4A 5301 Eugendorf, AUSTRIAPhone: (43) 6225-3241Fax: (43) 6225-2549Email: [email protected]: asyshitech.comAsys Hitech specializes in microplate readers,washers and dispensers.

BelgiumMAIA SCIENTIFICCipalstraat 3, B-2440 Geel, BELGIUMPhone: (32) 14-570-620Fax: (32) 14-570-621Email: [email protected]: maia-scientific.comMaia Scientific specializes in products for highcontent imaging & analysis.

CanadaHarvard Apparatus Canada6010 Vanden AbeeleSaint Laurent, Quebec H4S 1R9, CANADAPhone: 1-514-335-0792Toll Free: 1-800-361-1905 (Canada Only)Fax: 1-514-335-3482Email: [email protected]: harvardapparatus.caHarvard Apparatus Canada represents thesame product offerings as Harvard Apparatusin the United States, as well as vibration tablesand Biopac.

FranceHarvard Apparatus S.A.R.L.6 Avenue des AndesMiniparc - Bat. 891952 Les Ulis Cedex, FRANCEPhone: (33) 1-64-46-00-85Fax: (33) 1-64-46-94-38Email: [email protected] Apparatus S.A.R.L. represents thesame product offerings as Harvard Apparatusin the United States.

GermanyHugo Sachs Elektronik -Harvard Apparatus GmbHGruenstrasse 1March-Hugstetten D-79232, GERMANYPhone: (49) 0 7665-92-00-0Fax: (49) 0 7665-92-00-90Email: [email protected]: hugo-sachs.deHugo Sachs Elektronik is the industry leaderproviding advanced isolated organ and tissueperfusion systems as well as the PLUGSYSmodular amplifier system.

Hoefer, Inc.953 Indiana StreetSan Francisco, California 94107Phone: 1-415-282-2307Toll Free: 1-800-227-4750 (USA & Canada)Fax: 1-415-920-4494Email: [email protected]: hoeferinc.comHigh quality electrophoresis, blotting,quantitation and detection systems and more.

Global Distributioniv

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Hoefer is Back!

United StatesHoefer, Inc.Phone: + 1 800 227 4750 +1 415 550 3494www.hoeferinc.com

Taiwan: Unimed Healthcare Inc.Phone: + 886 2 27202215www.unimed.com.tw

MexicoDINELAB, S.A. DE C.V.Phone: + 52 55 5550 5065www.dinelab.com

New ZealandBio-Strategy Ltd.Phone: 0 800 DIAGNOSE (34 24 66)www.bio-strategy.com

Singapore: Research Instruments Ltd.Phone: + 65 6775 7284www.research-instruments.com.sg

Malaysia: Research Instruments Ltd.Phone: + 60 3 78 048600www.research-instruments.com.sg

Thailand: Becthai Bangkok ECC Ltd.Phone: + 66 2 615 2929www.becthai.com

LabChina Company LimitedPhone: + 86 20 38215851www.labchina.cn

Gene Company Ltd.Phone: + 85 2 896 6283www.genehk.com

Korea: Seoulin BiosciencePhone: + 82 2 478 5911www.seoulin.co.kr Fisher Scientific

Phone: + 1 800 766 7000www.fishersci.com

CanadaFisher Scientific CompanyPhone: + 1 800 234 7437www.fishersci.ca

AustraliaBio-StrategyPhone: 1 800 00 84 53www.bio-strategy.com

JapanLMS Co., Ltd.Phone: + 81 3 5842 4170

“Hoefer tiene gran productos.”

“La Qualité excellente et gamme la plus large.”

“Wir sind stolz, Hoefer Produkte zu verkaufen.”

“Il Hoefer ha di grandi prodotti per i nostri clienti.”

“Hoefer is a company with a great tradition of serving life scientists and we are happy to work with them.”

“Hoefer tillverkar utmärkta produkter som varar en livstid.”

ChinaGeneTimes Technology Inc.Phone: + 86 2154 26 2677www.genetimes.cn

Global Distribution

For up to date distribution information see www.hoeferinc.com

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Global Distribution

ArgentinaCientist S.A.Phone: + 54 11 48540804www.scientist.com

IsraelLumitron Electronic InstrumentPhone: + 972 2652 9898

TurkeyCommat Ltd.Phone: + 90 3124727417

IndiaBlue Star LimitedPhone: + 91 44 2815 8855www.bluestar.com

Czech RepublicCentral European Biosystems s.r.oPhone + 420 603533444

SlovakiaCentral European Biosystems s.r.oPhone: + 421 905534365Phone: + 42 0257 013 410

HungaryCentral European Biosystems KFTPhone: + 36 305279650

South AfricaUnited Scientific (Pty) Ltd.Phone: + 27 21 5925240

Italy:Pirovano and Partners

Phone: + 39 02 95 360 250

MozambiquePACIPhone: + 351 21 4155740

Denmark: Reactionlab ASPhone: + 45 7027 9595

www.reaction-lab.com

Netherlands: Isogen Life SciencePhone: + 31 346 550556www.isogen-lifescience.com

RussiaThe CIS and The BalticsPribori OYPhone: + 358 9 502 12 55www.egg.ru

Fisher Bioblock ScientificPhone: + 33 3 88 67 53 78www.bioblock.com

France Belgium Spain

SwitzerlandWitec AGPhone + 41 412505357www.witec.ch

UKFisher Scientific UK LtdPhone: + 44 (0) 1509 231166www.fishersci.co.uk

Ireland

PortugalPACI

Phone: + 351 21 4155742

AngolaPACI

Phone: + 351 21 4155740

GermanyNovoDirect GmbHPhone: + 49 (0) 7851 99457 0www.novodirect.de

GermanyFisher Scientific GmbHPhone: + 49 2304 932 5www.de.fishersci.com

Austria

Sweden: Reaction Lab Sverige ABPhone: + 46 18 14 9002

Finland: Reactionlab Finland OyPhone: + 358 2 410 1146www.reaction-lab.com

Norway: Reactionlab ASPhone: + 46 18 14 9002www.reaction-lab.com

Analytical Control S.p.A.Phone: + 39 02 6129241www.analyticalcontrol.it

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800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

New Productsfrom Hoefer

HOEFER SEMI-DRY TRANSFER UNITS useelectrophoresis to transfer proteins and nucleicacids from polyacrylamide or agarose gels ontonitrocellulose or nylon membranes.

TE 70X SERIESSEMI-DRY TRANSFER UNITS

SEE PAGES 27-28

The HOEFER IMMUNOBLOT AND IMMUNOBLOT XL makeit possible to assay the material on a transfer membranewith different antigens or antibody probes simultaneouslywithout cutting the membrane into strips. The membraneremains intact to preserve the correspondence betweendifferent lanes or positions on the gel.

IMMUNOBLOT & IMMUNOBLOT XLBLOTTING MANIFOLDS

SEE PAGE 32

DQ 300 FLUOROMETERDNA/RNA QUANTITATION

SEE PAGE 50

The HOEFER DQ 300FLUOROMETER is acompact, dual-channel unitthat delivers quick, easy, andaccurate fluorescencemeasurements with eitherlongwave UV or blueexcitation.

RCB-20REFRIGERATED & HEATEDCIRCULATION BATH

SEE PAGE 36

The HOEFER RCB-20REFRIGERATED &HEATED CIRCULATIONBATH provides efficienttemperature control for alltypes of electrophoresisand transfer techniques.

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New Products

CALIBER™HIGH QUALITYELECTROPHORESIS REAGENTS

SEE PAGES 39-42

HOEFER CALIBER™ ELECTROPHORESIS REAGENTSdeliver consistently outstanding results. A high standard ofpurity, rigorous analytical procedures, and carefullycontrolled manufacturing techniques ensure that HoeferCaliber reagents are the best quality buffer, gel casting, gelstaining and sample preparation chemicals available.

ULTima™IMAGING PLATFORM

SEE PAGES 43-48

The HOEFER ULTima™ is a convenientlyupgradable imaging platform incorporatingadvanced electronics and optics. The four modelsof gel documentation systems can handle themost demanding applications in Proteomics andGenomics without expensive laser scanningsystems.

HB-1000 HYBRIDIZATION OVEN &UVC 5000 ULTRAVIOLET CROSSLINKER

SEE PAGES 51-52

The HB 1000 HYBRIDIZATION OVEN is designed to createthe ideal environment for nucleic acid hybridization.

The HOEFER UVC 5000 ULTRAVIOLET CROSSLINKER is excellent forcross-linking DNA or RNA to membranes for multiple probings ofSouthern, Northern, dot, or slot blots; for DNA nicking in agarose gels;and for sterilizing samples. The UVC 5000 can be stacked on top ofthe HB 1000 hybridizer to save valuable lab space.

Hoefer Product Rangeviii

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Quantitation

DQ 300 FLUOROMETERDNA/RNA QUANTITATION

SEE PAGE 50

Electrophoresis

miniVESMALL FORMAT INTEGRATEDELECTROPHORESIS and BLOTTING UNIT

SEE PAGES 13-14

HOEFER MIGHTY SMALL IIMINI VERTICAL UNITS

SEE PAGES 15-16

DUAL COOLEDVERTICALELECTROPHORESIS UNITS

SEE PAGES 3-4

AIR COOLEDVERTICALELECTROPHORESIS UNITS

SEE PAGE 6

GRADIENT MAKERSSEE PAGE 20

RCB-20REFRIGERATED& HEATEDCIRCULATION BATH

SEE PAGE 36

MINI SUBMARINEELECTROPHORESIS UNITS

SEE PAGES 21-24

EV 265 CONSORTPOWER SUPPLY

SEE PAGE 37

CALIBERHIGH QUALITYREAGENTS

SEE PAGES 39-42

SE 100 PLATE MATEGLASS PLATE WASHER & STORAGE KIT

SEE PAGE 19

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Detection

MACROVUEUV TRANSILLUMINATORS

SEE PAGE 49

HB-1000 HYBRIDIZATION OVEN &UVC 5000 ULTRAVIOLET CROSSLINKER

SEE PAGES 51- 52

Imaging & Documentation

ULTimaIMAGING PLATFORMS

SEE PAGES 43 - 48

GEL DRYERSSEE PAGES 53-54

Blotting

IMMUNOBLOTBLOTTING MANIFOLDS

SEE PAGES 31-34

PS 2A200POWER SUPPLY

SEE PAGE 38


SEE PAGES 27-28

TRANSFER TANKSSEE PAGES 29- 30

1 VerticalElectrophoresis

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SE 660DUAL COOLEDVERTICALELECTROPHORESIS UNIT

SEE PAGE 4

SE 600X CHROMA™DUAL COOLEDVERTICALELECTROPHORESIS UNIT

SEE PAGE 3

Hoefer pioneered the vertical format for electrophoresis separations and continues to offer innovative designs and a variety of choices.

Hoefer SE 600 SERIES DUAL COOLEDTANK-STYLE VERTICAL UNITS run two orfour gels simultaneously at the sametemperature and a maximum of 112samples when using divider plates. A heatexchanger suspended in the buffer tankcools buffer evenly, producing excellentseparations with straight, sharp, well-definedbands.

Protein ElectrophoresisDual Cooled & Air-Cooled Vertical Electrophoresis

SE 600DUAL COOLEDVERTICALELECTROPHORESIS UNIT

SEE PAGE 4

SE 400 STURDIERAIR COOLEDVERTICALELECTROPHORESIS UNIT

SEE PAGE 6

SE 400 SERIES STURDIER VERTICAL UNITSare air-cooled, affordably priced and run onegel (or two, by using a divider plate).SE 400 Series units are the ideal choice forlabs requiring many units or applicationswhere temperature control is not crucial.

CASTERS FORVERTICALELECTROPHORESIS

SEE PAGE 7

Separate, simple- to-use GELCASTERS allow you to castmultiple identical gels at onetime.

2VerticalElectrophoresis

COMBS& SPACERS

SEE PAGE 10

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A wide array of well - formingCOMBS and SPACERS allowsyou to tailor gel configurationsto your needs.

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

SE 600X CHROMADeluxe Dual Cooled Vertical Unit

The Hoefer SE 600X, SE 600 and SE 660 are the high performance vertical units of choice in thousands of laboratories worldwide.

ORDERING INFORMATION

Includes:Upper Buffer ChamberChroma Safety Lid w/ High Voltage LeadsChroma Lower Buffer ChamberHeat ExchangerGlass Plates 18 x 16 cm - 3 matched pairsClamp Assemblies, 16 cm - 4 pcsCams - 6 pcsSlotted Gaskets for UBC - 2 pcsDual Gel Casting Stand with Leveling BaseSpirit LevelLaminated Gaskets for Caster - 2 pcsBuffer DamSpacer-Mate - Alignment TemplateWonder Wedge - Plate Separation ToolGel SealCombs, 15-Well - 2 pcsSpacers, 1.5-mm thick (2-cm wide) - 4 pcs

SE 600X CHROMAItem# SE600X-15-1.5

TECHNICAL SPECIFICATIONS

Spacers0.75 mm, 1.0 mm, 1.5 mm

Maximum Power Settings1000 V, 500 mA, 50 W

Operating ConditionsMaximum Temperature: 45 °CEnvironmental Operating Conditions:Indoor Use: 4 - 40 °CHumidity: up to 80%Altitude: up to 2000 m

Safety CertificationsEN61010-1UL3101-1CSA C22.2 1010.1CE

Glass Plate Size18 x 16 cm or 18 x 8 cm

Unit Dimensions (W x H x D):32 x 29 x 14 cm (12.5" x 11.5" x 5.5")

Rounded contours allow for ease in handling, lifting, introducing sample, and disassembling.

The striking SE 600X CHROMA is the latest addition to the Hoefer SE 600 Vertical Series.

Produces straight lanes and sharp, well- defined bands.

Runs as many as four gels (maximum 112 samples) at one time under identical conditions.

Accepts gels 8-cm or 16-cm long.

Accommodates denaturing and native polyacrylamide gels, and the seconddimension of 2-D electrophoresis.

A wide array of accessories enables you to tailor gel configurations to your needs.

Max. Glass Plate Size18 x 16 cm 1-4

GELS

Runs gels at temperatures from 1 to 45 °C.


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DIVIDER PLATES DOUBLE GEL CAPACITY

Run as many as four 0.75-, 1.0- or 1.5-mm thick gels on SE 600Series units and two gels on the SE 400 Series at one time

under identical conditions.

How it works: Fit a divider plate between the glass plates thatmake up a single sandwich. Put spacers on each side of the

divider plate to form two gel cavities for pouring gels. This two-gel“club sandwich” fits into the same casting stand and seals perfectly.

Divider Glass Plate(positioned between the outer plates)

Outer Glass Plates

Spacers

Clamp

SE 600 & 660Standard Dual Cooled Vertical Units

Order two SE 511 Combs & two sets of24-cm long Spacers separately, see page 10.

Includes:Upper Buffer ChamberSafety Lid w/ High Voltage LeadsSE 660 Lower Buffer ChamberHeat ExchangerGlass Plates 18 x 24 cm - 3 matched pairsClamp Assemblies, 8 cm - 4 pcsClamp Assemblies, 16 cm - 4 pcsCams - 6 pcsSlotted Gaskets for UBC - 2 pcsDual Gel Casting Stand with Leveling BaseSpirit LevelLaminated Gaskets for Caster - 2 pcsBuffer DamSpacer-Mate - Alignment TemplateWonder Wedge - Plate Separation ToolGel Seal

Item# SE660


Spacers: 0.75 mm, 1.0 mm, 1.5 mm

Maximum Power Settings: 1000 V, 500 mA, 50 W

Operating Conditions:Maximum Temperature: 45 °CEnvironmental Operating Conditions:Indoor Use: 4 - 40 °CHumidity: up to 80%Altitude: up to 2000 m

Safety CertificationsEN61010-1, UL3101-1, CSA C22.2 1010.1 and CE

Glass Plate SizeSE 600: 18 x 16 cm or 18 x 8 cmSE 660: 18 x 24 cm, 18 x 16 cm or 18 x 8 cm

Unit Dimensions (W x H x D):SE 600: 32 x 29 x 14 cm (12.5" x 11.5" x 5.5")SE 660: 32 x 37 x 14 cm (12.5" x 14.5" x 5.5")

Produce straight lanes and sharp,well-defined bands.

Run up to four gels (maximum 112 samples) atone time under identical conditions.

SE 600 accepts gels 8-cm and 16-cm long.SE 660, taller, but essentially the same design as theSE 600, accepts gels 8-, 16- and 24-cm long.

Accommodate denaturing and nativepolyacrylamide gels, and 2-D electrophoresis.

A wide array of accessories allows you to tailorgel configurations to your needs.

SE 660 accommodates 24 cm gels for increasedresolution.

Item# SE600

Order two SE 511 Combs & two sets of16-cm long Spacers separately, see page 10.

SE600 ORDERING INFORMATION

Includes:Upper Buffer ChamberSafety Lid w/ High Voltage LeadsSE 600 Lower Buffer ChamberHeat ExchangerGlass Plates 18 x 16 cm - 3 matched pairsClamp Assemblies, 16 cm - 4 pcsCams - 6 pcsSlotted Gaskets for UBC - 2 pcsDual Gel Casting Stand with Leveling BaseSpirit LevelLaminated Gaskets for Caster - 2 pcsBuffer DamSpacer-Mate - Alignment TemplateWonder Wedge - Plate Separation ToolGel SealCombs, 15-Well - 2 pcsSpacers, 1.5-mm thick (2-cm wide) - 4 pcs

Item# SE600-15-1.5

Includes: The same as above withoutthe combs & spacers.


GELS


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Wide-Mini Electrophoresis Systems

The HOEFER SE 640 VERTICALELECTROPHORESIS UNIT is a wide-minisystem, ideal for rapid screening ofmultiple samples. With the accessorydivider plates you can complete a run ofup to 112 samples in as little as 40minutes!

Permits rapid screening of up to 112samples at one time.

Design permits gels to be surroundeduniformly by buffer so heat dissipation isefficient. Bands are sharp and lanes arestraight.

Accommodates denaturing or nativepolyacrylamide gels.

Run up to four 2-D gels using IEFstrips or tube gels.

Ideal for optimizing 2DE protocols.



Maximum Power Settings:1000 V, 500 mA and 50 W


Safety CertificationsEN61010-1, UL3101-1, CSA C22.2 1010.1, CE

Glass Plate Size: 18 x 8 cm

Unit Dimensions (W x H x D):32 x 29 x 14 cm (12.5 x 11.5 x 5.5 in)


Includes:SE 640 Lower Buffer ChamberSE 640 Upper Buffer ChamberGlass Plates 18 x 8 cm – 3 matched pairs8 cm Clamp Assemblies – 4 pcsCams – 6 pcsDual Casting Stand w/Leveling BaseLaminated Gaskets for Casting Stand – 2 pcsSlotted Gaskets for UBC – 2 pcsBuffer DamSpacer-Mate alignment templateWonder Wedge plate separation toolGel SealSpirit LevelSE 600/660 Lid15-Well Combs – 2 pcs1.5-mm Thick Spacers (2-cm wide) – 4 pcs

SE 640 Vertical Electrophoresis Unit, CompleteItem# SE640-15-1.5

Max. Glass Plate Size18 x 8 cm 1-4 GELS

Includes:SE 640 Lower Buffer ChamberSE 640 Upper Buffer ChamberGlass Plates 18 x 8 cm – 3 matched pairs8 cm Clamp Assemblies – 4 pcsCams – 6 pcsDual Casting Stand w/Leveling BaseLaminated Gaskets for Casting Stand – 2 pcsSlotted Gaskets for UBC – 2 pcsBuffer DamSpacer-Mate alignment templateWonder Wedge plate separation toolGel SealSpirit LevelSE 600/660 Lid

SE 640 Vertical Electrophoresis Unit, BasicItem# SE640 (without combs & spacers)

Order SE 511 Comb and two sets of 8-cmlong Spacers separately, see page 10.

UBC for SE 640, Item# SE6454LBC for SE 640, Item# SE6450Buffer Dam for SE 640, Item# SE6432

Most of the spare parts are shared with the largerSE 600 (see pages 8-10), EXCEPT for the following:

Accessories & Spare Parts

Hoefer has been fabricating scientificinstruments for decades. You will never seebubbles or gaps in our glue seams. We usethe correct environment, formula andtechnique to get perfect results every time.

The result is products that perform and lookthe best -- that’s our reputation.

CRAFTSMANSHIP AT ITS BEST!


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SE 400 Series Sturdier vertical gel units live up to their name. Affordably priced, the SE 400 and SE 410 are ideal for laboratories requiring several units and for applications where temperature control is not crucial.



Maximum Power Settings:500 V @ 40 mA, 30 mA per gel, 20 W


Safety CertificationsEN61010-1,UL3101-1,CSA C22.2 1010.1CE

Glass Plate SizeSE 400: 18 x 16 cmSE 410: 18 x 24 cm

Unit Dimensions (W x H x D):SE 400: 24 x 28 x 15 cm (9.5" x 11" x 6")SE 410: 24 x 36 x 15 cm (9.5" x 14.2" x 6")

Order SE 511 Comb and a set of 24-cm longSpacers separately, see page 10.


Includes:Upper Buffer ChamberSafety Lid with ElectrodesLower Buffer Chamber & Casting Stand on Leveling Base with High Voltage LeadsGlass Plates, 18 x 24 cm - matched pairClamp Assemblies, 8 cm - 2 pcsClamp Assemblies, 16 cm - 2 pcsCams - 2 pcsSlotted Gasket for UBCSpirit LevelLaminated Gasket for Casting StandSpacer-Mate - Alignment TemplateWonder Wedge - Plate Separation ToolGel Seal

Item# SE410 (without combs & spacers)

Max. Glass Plate Size18 x 24 cm

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Includes:Upper Buffer ChamberSafety Lid with ElectrodesLower Buffer Chamber & Casting Stand on Leveling Base with High Voltage LeadsGlass Plates, 18 x 16 cm - matched pairClamp Assemblies, 16 cm - 2 pcsCams - 2 pcsSlotted Gasket for UBCSpirit LevelLaminated Gasket for Casting StandSpacer-Mate - Alignment TemplateWonder Wedge - Plate Separation ToolGel SealComb, 15-WellSpacers, 1.5-mm thick (2-cm wide) - 2 pcs

Item# SE400-15-1.5


1-2GELS

Sturdier™ Air-Cooled Vertical ElectrophoresisSE 400 Series

The rugged injection-moldedconstruction is durable andassembles easily.

SE 400 runs one 16-cm long gel.SE 410, the taller version of the SE 400,runs one 24-cm long gel.

Run two gels with the addition of adivider plate.

Built-in casting stand seals leak-free.

Choose from a wide variety ofaccessories.

Plates, clamps, spacers andcombs are interchangeablewith the SE 600.


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Gel CastersFor SE 600 & SE 400 Series Vertical Electrophoresis

Multiple Gel Casters allow you to cast several uniform or gradient gels at a time under identical conditions.

Order SE 511 Combs and sets of Spacersseparately, see page 10.


Includes:Caster Body with GasketFace PlateClamps - 4 pcsGlass Plates, 18 x 16 cm - 10 matched pairsFiller Sheets - 5 pcsWax Paper - 100 sheets, precutSpacer-Mate - Alignment TemplateThumbscrews - 2 pcsFiller PlugBlack Vinyl CapGel SealSpirit Level

SE 615 Multiple Gel CasterItem# SE615

Order SE 511 Combs and sets of Spacersseparately, see page 10.


Includes:Caster Body with GasketFace PlateClamps - 4 pcsGlass Plates, 18 x 16 cm - 4 matched pairsFiller Sheets - 5 pcsWax Paper - 100 sheets, precutSpacer-Mate - Alignment TemplateThumbscrews - 2 pcsFiller Plug SetBlack Vinyl CapGel SealSpirit Level

SE 675 Four Gel CasterItem# SE675

Order Glass Plates, SE 511 Combs and setsof Spacers separately, see page 10.


Includes:Caster Stand with 2 Cradles & Leveling FeetLaminated Gaskets - 2 pcs

SE 6015 Dual Gel CasterItem# SE6015

SE 615 casts up to ten 8- or 16-cm long gels.

Cast uniform or gradient gels. Pour uniform gels directly into the top of thecasting chamber; pour gradient gels through an inlet port at the bottom.

SE 675 casts up to four 8- or 16-cm long gels.

Easy to use and leak-free.

Multiple Gel CastersMultiple Gel Casters

Easy to use and leak-free.

Glass sandwiches seal into the castingcradle with a simple twist of the cams.

Casts two 8-, 16-, or 24-cm long gelsat a time.

SE 6015 Dual Gel Caster is part of thepackage when you order an SE 600 Seriesunit. It can also be ordered separately.

Dual Gel CasterDual Gel Caster

Max. GlassPlate Size

18 x 16 cm up to10 GELS

Max. Glass Plate Size18 x 16 cm up to

4 GELS Max. Glass Plate Size18 x 24 cm

1-2GELS

Silicone Filler Plug (for SE 615)Item# SE618

Silicone Filler Plug Set (for SE 675)Item# SE678

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)


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Clamp & Cam KitIncludes:Clamp Assembly 16 cm - 4 pcsCams Black - 8 pcsItem# SE6003UK (not pictured)

Lid for SE 600X ChromaItem# SE6056X

Clamp Assembly8 cm - 2 pcsItem# SE6403U

Clamp Assembly 16 cm - 2 pcsItem# SE6003U

Buffer DamItem# SE6032

Heat Exchangerfor SE 600 SeriesItem# SE6160

Cams Black - 4 pcsItem# SE6005L

Slotted Gaskets - 2 pcsfor UBCItem# SE6008B

Upper Buffer Chamber (UBC)for SE 600 SeriesItem# SE6054


SE 600 Vertical Electrophoresis

Lower Buffer Chamber (LBC)for SE 660Item# SE6650

Lower Buffer Chamber (LBC)for SE 600Item# SE6150

Lid for SE 600/660Item# SE6056

Lower Buffer Chamber (LBC)for SE 600X ChromaItem# SE6150X

Laminated Gaskets - 2 pcsfor Casting StandItem# SE6009

Casting StandIncludes:Caster Stand with 2 CradlesLeveling FeetLaminated Gaskets - 2 pcsItem# SE6015

Spirit LevelItem# SER11

Glass Plates & SpacersSee page 10

18 x 8 cm (WxH)Gel Sandwich

18 x 24 cm(WxH)Gel Sandwich

18 x 16 cm(WxH)GelSandwich

SE 400 UBCItem# SE4154

SE 400 LBCItem# SE4151

SE 400 LidItem# SE4156

SE 410 LidItem# SE416


GLASS PLATES

18 x 8 cm RegularItem# SE6402 (2 pcs)

18 x 8 cm Low FluorescenceItem# SE6402LF (2 pcs)

18 x 8 cmDivider Plate, NotchedItem# SE6402D







Slotted Gasket(for SE 400 UBC)Item# SE4008B

Laminated Gasket(for SE 400 LBC)

Item# SE4009

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& Spare PartsSE 600 & SE 400 Vertical Electrophoresis

GEL SANDWICH COMPARISON

High-VoltageSafety Lead SetItem# SE6056-HV


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Item#SE511-10-.75SE511-10-1.0SE511-10-1.5SE511-12-.75SE511-12-1.0SE511-12-1.5SE511-15-.75SE511-15-1.0SE511-15-1.5SE511-20-.75SE511-20-1.0SE511-20-1.5SE511-28-.75SE511-28-1.0SE511-28-1.5SE511-BKA

Thickness# ofWells

WellWidth Qty.

0.75 mm1.01.50.751.01.50.751.01.50.751.01.50.751.01.5

101010121212151515202020282828

8.3 mm8.38.37.67.67.65.75.75.74.14.14.12.72.72.7

1111111111111111

Standard Combs come attached to a standardback, which produces maximum well depths of25 mm, except for the 28 well combs whichproduce well depths of 15 mm.

Adjustable Comb Back(Converts 25-mm deep comb to 10 or 15-mm depth)

Item#SE511-R-.75SE511-R-1.0SE511-R-1.5SE511-DR-.75SE511-DR-1.0SE511-DR-1.5

Thickness# ofWells

WellWidth Qty.

0.75 mm1.01.50.751.01.5

1/11/11/11/21/21/2

121/6 mm121/6121/6113/6113/6113/6

111111

Preparative Combs have adjustable comb backs(10-, 15- and 25-mm depth).

All spacers are molded fromdurable plastics for highchemical resistance andoptimum tensile strength.

Item#SE6419-2-.75SE6419-2-1.0SE6419-2-1.5SE6119-2-.75SE6119-2-1.0SE6119-2-1.5SE6619-2-.75SE6619-2-1.0SE6619-2-1.5SE6118-2-1.0SE6118-2-1.5

Thickness Length Width Qty.0.75 mm1.01.50.751.01.50.751.01.51.01.5

8 cm881616162424241616

2 cm2222222211

22222222222

2 cm Width

1 cmWidth

1.5-mmThick

1.0 -mmThick

0.75-mmThick

All 1.0 mmSpacers arewhite for easyidentification.

COMBS SPACERS

Our combs arefabricated from plasticsthat are highly resistantto acids, bases, and allcommonly used buffersystems. They arelightweight and virtuallyunbreakable when usedwith reasonable care.

Gel SealItem# SE6070

Clamp Thumbscrews - 12 pk(for 8 or 16 cm Clamp)Item# SE6003U-2

Wonder WedgePlate Separation ToolItem# SE1514

11 Small Format VerticalElectrophoresis

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Protein ElectrophoresisSmall Format Vertical Electrophoresis

12Small Format VerticalElectrophoresis

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Hoefer’s small format vertical electrophoresis systems require reduced gel andbuffer volumes and shorter separation times than the standard sized units.

Alumina Plates and Glass PlatesSEE PAGE 18

SE 300 miniVESMALL FORMAT INTEGRATEDELECTROPHORESIS and BLOTTING UNIT

SEE PAGE 13

The SE 300 MINI VERTICAL UNIT allows you tocast, run, and blot two gels in 10 x 10.5 cm glassplates in one convenient instrument. Tank-formatpassive cooling assures the temperature uniformityrequired to achieve straight lanes and flat bands.

SE 260HOEFER MIGHTY SMALL IIMINI VERTICAL UNIT

SEE PAGE 15

The SE 250 and SE 260 MIGHTY SMALLVERTICAL ELECTROPHORESIS UNITS run twomini gels at a time. SE 250 runs gels cast in10 x 8 cm glass plates; the SE 260, in 10 x 10.5cm glass plates. The small formats combinedwith efficient cooling allow rapid screening ofproteins and nucleic acids.

CASTERS FORSMALL FORMAT VERTICALELECTROPHORESIS

SEE PAGE 17

Separate, simple-to-use GELCASTERS allow you to castmultiple gels at one time underuniform conditions.

A wide array of well-forming COMBS ANDSPACERS allows you to tailor gelconfigurations to your needs.SEE PAGE 18

SE 250HOEFER MIGHTY SMALL IIMINI VERTICAL UNIT

SEE PAGE 15


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Small Format Integrated Electrophoresis and Blotting Unit


Includes:Lower Buffer Chamber (Tank)Gel Modules with Gaskets – 2 assembliesSafety Lid with High Voltage LeadsGlass Plates, Rectangular, 10 x 10.5 cm – 3 pcsGlass Plates, Notched, 10 x 10.5 cm – 3 pcsCombs, 10-well – 2 pcsT-Spacers, 1.0-mm thick – 4 pcsWell-Locating Decals – 2 pcsWonder Wedge Plate Separation ToolGel Seal

SE 300 miniVEItem# SE300-10A-1.0



Maximum Power Settingsfor electrophoresis: 500 V and 25 W per modulefor blotting: 400 mA and 15 W per module


Safety CertificationsEN61010-1, UL3101-1, CSA C22.2 1010.1, CE

Glass Plate Size: 10 x 10.5 cm or 10 x 8 cm

Unit Dimensions (W x H x D):19.2 x 17.2 x 18.8 cm (7.6" x 6.8" x 7.4")

Cast, run, and blot two long mini gels in one instrument.

The HOEFER miniVE unit allows you to perform both electrophoresis andelectrotransfer in one sturdy, compact unit.

Quick, excellent results use minimal buffer.

Efficient cooling produces gels with straight lanes and flat bands.

Cast your own or use pre-cast gels from a variety of manufacturers.

Transfer 4 gels in 45 minutes with only 300 ml buffer using optional SE 302 Blot Modules.

1-2GELS

Sturdy, safe, and easy to use.

Max. Glass Plate Size10 x 10.5 cm 1-2

GELS

Includes:Blot ModuleFoam Sponges 6 mm thick - 3 pcsBlotter Paper - 25 sheets

SE 302 Blot ModuleItem# SE302

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)


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miniVE Lid w/high-voltage leadsItem# SE306

miniVE Tank (LBC)Item# SE308

miniVE Clamp Screws (5 pcs)Item# SE311

miniVE Clamp AssemblyItem# SE310

miniVE Sealing GasketItem# SE313

miniVE Banana Plug Kit(2 plugs, 2 nuts, 4 washers)Item# SE314

Gasket, 100 cmItem# FH2208

Spare PartsminiVE

miniVE UBC Assemblywith Gasket & Banana Plug KitItem# SE309

miniVE Sealing Plate Assembly(includes Sealing Gasket)Item# SE312


GELS


HoeferMighty Small™ II

Small Format Vertical Electrophoresis






Glass Plate SizeSE 250: 10 x 8 cmSE 260: 10 x 10.5 cm or 10 x 8 cm

Unit Dimensions (W x H x D):SE 250: 16.5 x 16 x 16 cm (6.5" x 6.3" x 6.3")SE 260: 16.5 x 18 x 16 cm (6.5" x 7.1" x 6.3")

The Hoefer SE 250 combines the advantages of small format and efficient coolingfor rapid screening of proteins and nucleic acids. The Hoefer SE 260 runs tallergels with the same efficient cooling and compact design.

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SE 250 runs two gels cast in 10 x 8 cm glass plates.

SE 260 runs two gels cast in 10 x 10.5 cm glassplates; 30% more resolving distance for complexsamples.

Quick to assemble; easy and safe to use.

Requires minimal buffer.

Efficient cooling ensures flat bands.

Run as many as 30 sample lanes.

Choose from a wide variety of accessories.

Includes:Lower Buffer ChamberUpper Buffer Chamber / Cooling CoreSafety Lid with High Voltage LeadsCasting Cradle w/Sealing Gasket SetCasting Clamp Assemblies – 2 pcsCams – 4 pcsGlass Plates, Rectangular, 10 x 10.5 cm – 10 pcsAlumina Plates, Notched, 10 x 10.5 cm – 2 pcsSpring Clamps, Red – 4 pcsCombs, 10-well – 2 pcsT-Spacers, 0.75-mm thick – 4 pcsWell-Locating Decals – 2 pcsGel Seal

Item# SE260-10A-.75


Includes:Lower Buffer ChamberUpper Buffer Chamber / Cooling CoreSafety Lid with High Voltage LeadsCasting Cradle w/Sealing Gasket SetCasting Clamp Assemblies – 2 pcsCams – 4 pcsGlass Plates, Rectangular, 10 x 8 cm – 10 pcsAlumina Plates, Notched, 10 x 8 cm – 2 pcsSpring Clamps, Red – 4 pcsCombs, 10-well – 2 pcsT-Spacers, 0.75-mm thick – 4 pcsWell-Locating Decals – 2 pcsGel Seal

Item# SE250-10A-.75


GELS


SE 260SE 250

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)


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Spare Parts

Foam Gasket, 61 cmItem# SE208

UBC/Cooling Core(includes Foam Gasket)Item# SE254B

LBC for SE 250Item# SE255

SE 250 or SE 260 Lidwith High-Voltage LeadsItem# SE256

Decal, Well-LocatingItem# SE212

QuickFit connectors,female 1/4" (2 pcs)Item# QF1/4

LBC for SE 260Item# SE255D

Hardware Repair Kit - Not Pictured(2 banana plugs, 6 washers, 2 nuts,Teflon tubing 3" (3 pcs), 4 acetal rivets)Item# SE250RK-1

Spring Clamps, Red,Asymmetric (4 pcs)Item# SE252

Mighty Small II, Small Format Vertical Electrophoresis

High-Voltage LeadsItem# SE6056-HV

QuickFit connectors,female 3/8" (2 pcs)Item# QF3/8

QuickFit connectors,male 3/8" (2 pcs)Not PicturedItem# QFX3/8

Max. GlassPlate Size

10 x 8 cm

Gel CastersSmall Format Vertical Electrophoresis

Multiple Gel Casters allow you to cast several uniform or gradient gels at a time under identical conditions.


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Combs & Spacers sold separately(see pages 18-19)

Includes:Caster Body with GasketFace Plate with Inlet PortGlass Plates, Rectangular, 10 x 8 cm – 10 pcsAlumina Plates, Notched, 10 x 8 cm – 4 pcsSpring Clamps, Red – 2 pcsWax Paper – 100 sheetsSpace-Saver PlatePolycarbonate Filler Sheets – 5 pcsBlack Vinyl CapFiller Plug Set

SE 275 Four Gel CasterItem# SE275


Combs & Spacers sold separately(see pages 18-19)

Includes:Caster Body with GasketFace Plate with Inlet PortGlass Plates, Rectangular, 10 x 8 cm – 20 pcsAlumina Plates, Notched, 10 x 8 cm – 10 pcsSpring Clamps, Red – 2 pcsWax Paper – 100 sheetsSpace-Saver PlatePolycarbonate Filler Sheets – 5 pcsBlack Vinyl CapFiller Plug Set

SE 215 Multiple Gel CasterItem# SE215


Includes:Casting CradleCasting Clamp Assemblies – 2 pcsSealing Gasket SetCams – 4 pcs

SE 245 Dual Gel CasterItem# SE245

Uses the same rugged and effectiveclamp/cam casting method as usedwith the SE 400 and SE 600.

Accommodates both 10 x 8 cm and10 x 10.5 cm plates.

Cast one or two gels with the SE 245 DUALGEL CASTER.

Dual Gel CasterDual Gel Caster

Pour uniform gels from the top; pump gradient gels into an inlet port at thebottom of the unit.

Once cast, gels can be wrapped in plastic and refrigerated for several weeks.

SE 275 casts from 1 to 4 gels simultaneously; SE 215 casts as many as10 gels simultaneously.

Sandwiches fill at the same time at the same rate from the same solution.The result: identical gels.

Multiple Gel CastersMultiple Gel Casters

HOEFER’S SE 215 and SE 275 MULTIPLE GEL CASTERS allow you to cast severaluniform or gradient gels in 10 x 8 cm plates under identical conditions.

Glass plate sandwiches seal leak-free without the use of messy grease or tape.


1-4GELS 1-10

GELS

Clamp Thumbscrews (12 pcs), Item# SE6003U-2

Filler Plug Set for SE 275 (3 pcs), Item# SE279Filler Plug Set for SE 215 (3 pcs), Item# SE209

Casting Clamp Assembly, Item# SE249

Sealing Gasket Set, Item# SE246

Face Plate, Item# SE218

Wax Paper Sheets (100 pcs), Item# SE201Filler Sheets, Polycarbonate (5 pcs), Item# SE213

Cams (4 pcs), Item# SE6005L

Space-saver Plate, Item# SE217

Red Inlet/Outlet Fitting, Item# XPO10

SE 215 and SE 275SE 215 and SE 275

SE 245SE 245

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

Accessories

Spring Clamps, Red, (4 pcs), Item# SE252Foam Gaskets, (61 cm), Item# SE208


GELS

All spacers are molded from durable plasticsfor high chemical resistance and optimumtensile strength.

Item#SE2119T-2-.75SE2119T-2-1.0SE2119T-2-1.5SE2619T-2-.75SE2619T-2-1.0SE2619T-2-1.5

Thickness Length Width Qty.0.75 mm1.01.50.751.01.5

8 cm8810.510.510.5

1 cm11111

222222

2 cm Width

1 cmWidth 1.5-mm

Thick

0.75-mmThick

All 1.0 mmT-Spacers are whitefor easy identification.

T-SPACERS


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AccessoriesSmall Format Vertical Electrophoresis

GLASS PLATES

10 x 8 cm (10 pcs)Rectangular Glass PlatesItem# SE202P-10

10 x 8 cm (5 pcs)Notched Glass PlatesItem# SE202GN-5

10 x 10.5 cm (5 pcs)Rectangular Glass PlatesItem# SE262P-5

10 x 10.5 cm (5 pcs)Notched Glass PlatesItem# SE262GN-5

ALUMINA PLATES

10 x 8 cm (10 pcs)Notched Alumina PlatesItem# SE202N-10

10 x 8 cm (1 pc)Notched Alumina PlatesItem# SE202N

10 x 10.5 cm (5 pcs)Notched Alumina PlatesItem# SE262N-5

COMBS

Item#SE211A-5-.75SE211A-5-1.0SE211A-5-1.5SE211A-9-1.0SE211A-10-.75SE211A-10-1.0SE211A-10-1.5SE211A-15-.75SE211A-15-1.0SE211A-15-1.5SE211A-18-1.0

Thickness# ofWells

WellWidth Qty.

0.75 mm1.01.51.00.751.01.50.751.01.51.0

5559*10101015151518*

13.0 mm13.013.05.84.84.84.82.92.92.92.9

11111111111

Combs: Standard

*Microtiter Spacing

Item#SE211A-R-.75SE211A-R-1.0SE211A-R-1.5

Thickness# ofWells

WellWidth Qty.

0.75 mm1.01.5

1/11/11/1

68/5 mm68/568/5

111

Combs: PreparativePreparative Combs form 1 large preparative well plus 1 small reference well.

51/3.868/5120/7.5

9.813.019.55.83.64.87.22.22.94.42.9

Well Volume(µl per 1 mm depth)

Well Volume(µl per 1 mm depth)


Wonder WedgePlate Separation ToolItem# SE1514

Gel Seal 1/4 oz.Item# SE6070

1.0-mmThick

At Hoefer we use only the best in materials to ensureperformance, quality and longevity. The lower buffer chamber ofa Mighty Small II unit is wired with platinum wire.

ONLY THE BEST!

SE 100 Plate Mate™

Glass Plate Washer & Storage Kit

The SE 100 PLATE MATE WASHER AND STORAGE KIT dramatically reduces glassplate loss due to chipping and cracking.

Glass plates fit securely into the plate holders’ grooves for safe washing,rinsing, and storage.

Stainless steel bail handles let you carry plate holders easily and set them safelyinto the washing bath.

Holds as many as twenty 10.5-cm tall plates with the optional SE 103 longplate adapter accessory.

Accommodates forty 8-cm tall or twenty 18-cm wide plates.


Long Plate Adapters - 2 pcsItem# SE103

Plate Holder w/Handle & Dust CoverItem# SE105

SE100 TECHNICAL SPECIFICATIONS

Materials:Tank & Tank Lid: High-density PolyethyleneHolders: PolypropyleneDust Covers: VinylHandles: Stainless Steel

Holders and AdaptersEach holder can hold either 10 SE 600 series glass plates in thelong direction, or 20 SE 250 glass plates in the short direction.An optional adapter can also be used to adapt a holder to hold10 SE 260 or miniVE glass plates.

Liquid Capacity of Tank7 L to cover 18 x 16 cm plates(for the SE 400, SE 600 Chroma or SE 600)or5 L to cover 10 x 8 cm and/or 10 x 10.5 cm plates(SE 250, SE 260, miniVE)Add more solution for larger plates, if required.

Operating Conditions: Maximum Temperature: 45 °C

Includes:Polypropylene Washing TankPlate Holders w/Handles & Dust Cover – 2 sets

Item# SE100

19 Glass Plate Washer& Storage Kit

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800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

All 4 ModelsInclude:Gradient Maker w/Teflon ValvesLuer Outlet FittingSupport RodAdapter Barbed Fitting22-gauge Needle

SG 50 Gradient Maker(50 ml total volume)Item# SG50



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20Gradient Makers

Milled of heavy acrylic plastic with high-quality, leak-freeTeflon valves.

HOEFER SMALL VOLUME SG SERIES GRADIENT MAKERS areideal for generating gradients of polyacrylamide, sucrose andcesium chloride. Choose from 15, 30, 50, or 100 ml sizes.

Flat base provides stability atop magnetic stirrers. Comes witha support rod for attaching to a ring stand.

Takes Luer or 4-mm ID tubing. With included adapters, canalso take 2-mm ID or 22-gauge tubing.

SG 100 is ideal for the Hoefer Mighty Small Gel Casters SE 275(for 4 gels) or SE 215 (for 10 gels).

Gradient MakersSG Series

A simple push-pull valve opens and closes the passage betweenthe reservoir and mixing chambers.

The 500 ml SG 500 GRADIENT MAKER is made of two cylindricalacrylic chambers joined and mounted on a flat acrylic base.

Suitable for forming polyacrylamide gradients or buffer gradientsused with medium to small chromatography columns.

Ideal for use with the SE 615 Multiple Gel Caster.

SG 15, 30, 50, 100 ORDERING INFORMATION

SG 100Gradient Maker(100 ml total volume)Item# SG100

Includes:Gradient Maker w/Push-Pull ValveWrap-and-Seal Hose Clamps — 4 pcs


SG 500 ORDERING INFORMATION

1.1

HE 99XMAX SUBMARINEELECTROPHORESIS UNIT

SEE PAGE 23

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21 Nucleic AcidElectrophoresis

Nucleic Acid Electrophoresis

Submarine Agarose Units

Because of their semi-rigid shape and the regular phosphate charge distribution along their length,DNA fragments separate by size in simple electrophoresis systems. Agarose is the preferred gel matrixused, which is easy to cast and has a good size separation range.

For separation of 100 bp to 20 kbp fragments, we recommend the Hoefer horizontal systems, HE 99XMAX SUBMARINE UNIT and HE 33 MINI SUBMARINE UNIT.

Nucleic acids can also be separated in vertical acrylamide gels, which have a much higher resolvingpower than agarose gels but a narrower size range of separation. For vertical separations of smallDNA fragments such as oligonucleotides up to 1000 bp, we recommend the HOEFER SE 300 miniVEor the SE 250 and SE 260 MIGHTY SMALL UNITS. For even further increased separation, werecommend the longer gels of the HOEFER SE 600 or SE 400 SERIES STANDARD VERTICALELECTROPHORESIS UNITS.

HE 33MINI SUBMARINEELECTROPHORESIS UNIT

SEE PAGE 22

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

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22Nucleic AcidElectrophoresis

HE 33 MiniSubmarine Agarose Electrophoresis Unit

Runs a 7 x 10 cm gel in as little as 5 minutes.

The HOEFER HE 33 MINI SUBMARINE AGAROSE UNIT is designed for veryfast separations of DNA restriction fragments in agarose gels. To achieveexcellent cooling, you simply fill the clear molded base with 50% ethyleneglycol coolant solution, seal it permanently, and place it in a freezer or ice bathuntil ready to use.

Running tray accepts two combs for up to 32 samples.

UV transparent running tray permits clear viewing of bands stained withethidium bromide without handling the gel.

Combs for forming sample wells are available with 8, 12, or 16 wells, in1.0 or 1.5 mm thickness.

HE 33 ORDERING INFORMATION

Includes:Buffer Chamber AssemblySafety Lid w/High Voltage LeadsRunning TrayCasting TrayFoam Gaskets – 4 pcsSpirit LevelComb, 1.5-mm thick, 8 wellsComb Back

HE 33 Mini Submarine Unit, CompleteItem# HE33-8-1.5


Maximum Buffer Volume: 250 ml

Maximum Power Settings500 V for 5 min or less, 500 mA, 15 W


Safety Certifications:EN61010-1, UL61010A-1, CSA C22.2 1010.1, CE

Gel Dimensions: 7 x 10 cm

Unit Dimensions (L x H x W):24 x 7 x 13 cm (9.5" x 2.8" x 5.2")

Max. Running Tray Size7 x 10 cm

Includes:Buffer Chamber AssemblySafety Lid w/High Voltage LeadsRunning TrayCasting TrayFoam Gaskets – 4 pcsSpirit Level

HE 33 Mini Submarine Unit, BasicItem# HE33BOrder HE31A Comb and HE31-BK CombBack separately.

HE 33 COMBS

Item#HE31A-P-1.0HE31A-P-1.5HE31A-8-1.0HE31A-8-1.5HE31A-12-1.0HE31A-12-1.5HE31A-16-1.0HE31A-16-1.5

Thickness# ofWells

WellWidth Qty.

1.0 mm1.51.01.51.01.51.01.5

1*1*8812121616

44/6 mm44/66.56.53.93.92.62.6

11111111

*1 prep/2 ref

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23 Nucleic AcidElectrophoresis

HE 99X Max™

Submarine Agarose Electrophoresis Unit

Casting and running trays are available for 15 x 20 cm, 15 x 15 cm, and15 x 10 cm gels.

The HOEFER HE 99X MAX offers a variety of gel lengths and comb sizes so thatyou can design the gel you need for optimum resolution of DNA fragments.

Durable, leak-proof casting trays are easy to use. Cell-foam strips seal theends securely.

Self-locating/centering guides in the UV transparent running trays preventgel sliding or floating.

You can tailor gel configurations with a variety of combs.


Includes:Buffer Chamber AssemblySafety Lid w/High Voltage LeadsRunning Tray, 15 x 20 cmCasting Tray, 15 x 20 cmFoam Gaskets – 4 pcsSpirit LevelComb, 1.5-mm thick, 15 wellsComb Back

HE 99X Max Submarine Unit, CompleteItem# HE99X-15-1.5

Includes:Buffer Chamber AssemblySafety Lid w/High Voltage LeadsSpirit Level

HE 99X Max Submarine Unit, BasicItem# HE99XOrder Casting Kit, HE91A Comb and HE91-BKComb Back separately.

Comb depth is adjustable.

Max. Running Tray Size15 x 20 cm

HE 99X COMBS

Item#HE91A-P-1.5HE91A-P-3.0HE91A-10-1.5HE91A-10-3.0HE91A-15-1.0HE91A-15-1.5HE91A-15-3.0HE91A-20-1.0HE91A-20-1.5HE91A-20-3.0HE91A-30-1.0

Thickness# ofWells

WellWidth Qty.

1.5 mm3.01.53.01.01.53.01.01.53.01.0

1*1*101015151520202030

113/10 mm113/109.79.77.17.17.14.74.74.73.0

11111111111


Maximum Buffer Volume: 1.2 liter



Safety Certifications:EN61010-1, UL61010A-1, CSA C22.2 1010.1, CE

Gel Dimensions:15 x 20 cm, 15 x 15 cm, or 15 x 10 cm

Unit Dimensions (L x H x W):36 x 14 x 18.2 cm (14.2" x 5.5" x 7.2")

*1 prep/2 ref

Order HE91-BK Comb Back separately.

Running Tray15 x 20 cm Item# HE92X-2015 x 15 cm Item# HE92X-1515 x 10 cm Item# HE92X-10

Running TrayItem# HE42-10

Casting TrayItem# HE45-10

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24Nucleic AcidElectrophoresis


Foam Gaskets (4 pcs)Item# HE48

High-Voltage Leads (2 pcs)Item# SE6056-HV

HE 33 Mini

Submarine Unit Accessories & Spare Parts

HE 99X Max

Top Fill Plug Kit(4 pcs)Item# HE38TP

Lid w/High Voltage LeadsItem# HE36

Comb Back + 2 screwsItem# HE31-BK

Electrode Replacement Kit (not pictured)Item# HE39

Foam Gaskets (4 pcs)Item# HE98X

15 x 20 cm Item# HE97X-2015 x 15 cm Item# HE97X-1515 x 10 cm Item# HE97X-10

BufferChamberAssemblyItem# HE90X

Electrode Replacement Kit (not pictured)Item# HE99XRK-1

Gel Casting KitRunning TrayCasting TrayFoam Gaskets - 4 pcsItem# HE47-10

Buffer ChamberAssemblyItem# HE30

Casting Traywith Foam Gaskets - 4 pc15 x 20 cm Item# HE95X-2015 x 15 cm Item# HE95X-1515 x 10 cm Item# HE95X-10

High-Voltage Leads (2 pcs)Item# SE6056-HV

Comb Back + 2 screwsItem# HE91-BK

Gel Casting KitRunning TrayCasting TrayFoam Gaskets - 4 pc

Lid w/High Voltage LeadsItem# HE96X


25 ProteinBlotting

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TE 62TRANSFER TANK

SEE PAGE 30

TE 22MIGHTY SMALLTRANSFER TANK

SEE PAGE 29

Hoefer pioneered the vertical format for electrophoresis separations and continues to offer innovative designs and a variety of choices.

Protein BlottingTE Series Transfer Systems

26ProteinBlotting

PR

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GElectrophoretic transfer of proteins is a much faster procedure than simple capillary or contact blotting methods.By directing electrical current through a transfer stack, blotting rates can be greatly accelerated.

Assays performed on transfer membranes are sensitive and require only small amounts of material. This is criticalwhen the species of interest is present in low concentrations or represents a minor fraction of the total sample.Moreover, since antibodies and other affinity reagents are often expensive, this can greatly reduce cost.

HOEFER TE SERIES TRANSFER TANKS and SEMI-DRY TRANSFER UNITS use electrophoresis to deliver excellenttransfers of proteins and nucleic acids from polyacrylamide or agarose gels onto nitrocellulose, PVDF or nylonmembranes.


SEE PAGES 27 & 28

TE 42TRANSFER TANKSEE PAGE 30

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

ProteinBlotting27

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TE 70X & TE 70XPSemi-Dry Transfer Units


Includes:Molded Base w/Pt-Nb Anode & High Voltage LeadsHinged Lid w/Stainless Steel CathodeBlotter Paper – 25 sheetsPorous Cellophane – 50 sheetsMylar™ Masks – 2 pcs

TE 70X Semi-Dry Transfer UnitItem# TE70X




Safety CertificationsEN61010–1CE

Gel Dimensions: up to 14 x 16 cm

Unit Dimensions (W x H x D):38 x 46 x 9 cm (15" x 18" x 3.5")

Units accept gels up to 14 x 16 cm, or two SE 260 mini gels side-by-side. Transfer twopolyacrylamide gels in a stack.

The TE 70X and TE 70XP SEMI-DRY TRANSFER UNITS use minimal buffer to transfer proteinsand nucleic acids from polyacrylamide and agarose gels in less than an hour. The TE 70XPhas the added convenience of a built-in power supply.

Economical to use. Require only a small amount of buffer.

Require minimal current. Do not generate the excessive heat that can dry out the transferstack and halt transfer or damage transfer units.

TE 70XP includes an intelligent built-in power supply which automatically monitorsthe transfer status, stopping the transfer before the stack overheats.

Max. Gel Size14 x 16 cm

Durable platinum-niobium and stainless steel electrodes assure contamination-free transfers.

Includes:Molded Base w/Pt-Nb Anode & Internal Power SupplyHinged Lid w/Stainless Steel CathodeBlotter Paper – 25 sheetsPorous Cellophane – 50 sheetsMylar Masks – 2 pcs

TE 70XP Semi-Dry Transfer UnitItem# TE70XP

Accessories:Mylar Masks – 4 pcs, Item# TE74Blotter Paper – 25 sheets, Item# TE76-1416Porous Cellophane – 50 sheets, Item# TE73

Widest range of systems to suit all your western blotting needs.

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

TE 70X

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TE 77X & TE 77XP

Semi-Dry Transfer Units

Units accept gels up to 21 x 26 cm, or four SE 260 minigels side-by-side. Transfer twopolyacrylamide gels in a stack.

The HOEFER TE 77X and TE 77XP SEMI-DRY TRANSFER UNITS have the same efficientdesign and outstanding features as the TE 70X and TE 70XP, but accommodate larger gels.

Produce uniform and even transfer results quickly.

Require only a small amount of buffer.

Durable platinum-niobium and stainless steel electrodes assurecontamination-free transfers.

TE 77XP includes an intelligent built-in power supply which automaticallymonitors the transfer status, stopping the transfer before the stack overheats.

Require minimal current. Do not generate the excessive heat that can dry out thetransfer stack and halt transfer or damage the unit.


Includes:Molded Base w/Pt-Nb Anode & High Voltage LeadsHinged Lid w/Stainless Steel CathodeBlotter Paper – 25 sheetsPorous Cellophane – 50 sheetsMylar Masks – 2 pcs

TE 77X Semi-Dry Transfer UnitItem# TE77X

Max. Gel Size21 x 26 cm

Includes:Molded Base w/Pt-Nb Anode & Internal Power SupplyHinged Lid w/Stainless Steel CathodeBlotter Paper – 25 sheetsPorous Cellophane – 50 sheetsMylar Masks – 2 pcs

TE 77XP Semi-Dry Transfer UnitItem# TE77XP

Accessories:Mylar Masks – 4 pcs, Item# TE78Blotter Paper – 25 sheets, Item# TE76Porous Cellophane – 50 sheets, Item# TE73


Maximum Power Settings50 V, 450 mA, 22.5 W


Safety CertificationsEN61010–1CE

Gel Dimensions: up to 21 x 26 cm

Unit Dimensions (W x H x D):38 x 46 x 9 cm (15" x 18" x 3.5")

TE 77XP

TE 22Mighty Small Transfer Tank

29 ProteinBlotting

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ORDERINGINFORMATION

TECHNICALSPECIFICATIONS


Operating ConditionsMax. Temperature: 45 °CEnvironmentalOperating Conditions:Indoor Use: 4 - 40 °CHumidity: up to 80%Altitude: up to 2000 m

Safety CertificationsEN61010-1, UL3101-1,CSA C22.2 1010.1, CE

Capacityup to four 9 x 10 cm gels(SE 260-sized)

Unit Dimensions(W x H x D):14 x 24 x 16.5 cm(5.5" x 9.5" x 6.5")

Includes:Lower Chamber w/Cooling ChannelSafety Lid w/High Voltage LeadsElectrode Panels – 2 pcsCassettes – 4 pcsFoam Sponges, 6-mm Thick – 4 pcsFoam Sponges, 3-mm Thick – 8 pcsCassette HookBlotter Paper – 25 sheets

TE 22Mighty Small Transfer TankItem# TE22

Max. Gel Size9 x 10 cm 1-4

GELSTransfers as many as four small gels,up to 9 x 10 cm in less than an hour.

The compact TE 22 MIGHTY SMALLTRANSFER TANK quickly and efficientlytransfers proteins and nucleic acidsfrom small gels to nylon, nitrocelluloseor PVDF membranes.

Built-in alumina-covered coolingchannel provides excellenttemperature control with no morethan a 5 °C temperature increaseduring a typical run.

Superior design provides a uniformelectric field – the key to even transfers.

No gel distortion. Grooves inside theunit hold cassettes in place, applyingequal pressure across the gel.

Cassettes are easy to load and handle.


Electrode PanelItem# TE23

Lower Chamber w/CoolingChannel

Item# TE27

Safety Lidw/High Voltage Leads

Item# TE29

Cassette w/SpongesItem# TE24

ReplacementHigh Voltage LeadsItem# SE6056-HV

Repair Kit, Fittings(Not Pictured)

Item# TE22RK-1

Foam Sponges

3-mm Thick – 4 pcsItem# TE25F-1/8

6-mm Thick – 4 pcsItem# TE25

Blotter Paper, 50 sheets(Not pictured)Item# TE26

Cassette w/SpongesItem# TE44H

Foam Sponges

3-mm Thick – 4 pcsItem# TE45F-1/8

Electrode Panel,BlackItem# TE43BK

Electrode Panel,GreyItem# TE43GY

30ProteinBlotting


Maximum Power Settings: 100 V, 2 A, 200 W

Operating Conditions


CapacityTE 62: up to four 15 x 21 cm gelsTE 42: up to four 15 x 21 cm gels (without cooling); up to two 15 x 21 cm gels (with cooling)

Unit Dimensions (W x H x D):SE 600: 28 x 16.5 x 32 cm (11" x 6.5" x 12.5")SE 660: 28 x 13 x 30.5 cm (11" x 5.1" x 12")

The TE 62 TRANSFER TANK quickly and evenly transfersproteins and nucleic acids from polyacrylamide or agarosegels onto nitrocellulose, PVDF or nylon membranes.A cooling chamber built into the base of the unit providesefficient temperature control for optimal transfer results.

Transfers up to four 15 x 21 cm gels or as many assixteen 7 x 10 cm mini-gels.

Built-in alumina-covered cooling channel together withexternal cooling waterbath (see page 36) ensuresconsistent transfers.

Perform transfers in one hour.

Easy-to-load cassettes hold gels evenly – no gel distortion.

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TE 62 & TE 42Transfer Tanks

Transfers up to four 15 x 21 cm gels at once withoutcooling, or up to two gels at once with optional cooling

Use the optional TE 47 Heat Exchanger and externalcooling waterbath to control buffer temperature duringtransfer.

The TE 42 TRANSFER TANK differs from the TE 62 only inthe cooling system.

Includes:Lower Chamber w/Cooling ChannelSafety Lid w/High Voltage LeadsElectrode Panels – 2 pcsCassettes – 4 pcsFoam Sponges, 6-mm Thick – 4 pcsFoam Sponges, 3-mm Thick – 8 pcsCassette HookBlotter Paper – 25 sheets

Item# TE62


GELS


Heat ExchangerItem# TE47

Lower Chamberw/Cooling Channel,for TE 62Item# TE67

Lower Chamber,for TE 42Item# TE56

Safety Lidw/High Voltage LeadsItem# TE49

ReplacementHigh Voltage LeadsItem# SE6056-HV

Blotter Paper50 sheetsItem# TE46

Dacron Sponges,6-mm Thick – 2 pcsItem# TE45

Not Pictured

6-mm Thick – 4 pcsItem# TE45F

TE62 ORDERINGINFORMATION

Includes:Lower ChamberSafety Lid w/High Voltage LeadsElectrode Panels – 2 pcsCassettes – 2 pcsFoam Sponges, 6-mm Thick – 2 pcsFoam Sponges, 3-mm Thick – 4 pcsCassette HookBlotter Paper – 25 sheets

Item# TE42


GELS

TE42 ORDERINGINFORMATION

Max. Temperature: 45 °CEnvironmental Operating Conditions:Indoor Use: 4 - 40 °C, Humidity: up to 80%,Altitude: up to 2000 m

1.1

IMMUNOBLOT XL SEE PAGE 32

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31 Immunoblotting

ImmunoblottingImmunoBlot™ and Deca-Probe™ Blotting Manifolds

PR 150DECA-PROBE

SEE PAGE 33

Transfer of proteins or nucleic acids, fractionated by gel electrophoresis, to animmobilizing membrane surface increases the sensitivity of a wide range ofdetection methods, reduces time of analysis, and makes sequential probing possible.In particular, it has made possible the use of immunological procedures that are notpractical to carry out on a gel or in a tube or plate.

The HOEFER IMMUNOBLOT AND IMMUNOBLOT XL and HOEFER PR 150 DECA-PROBE make it possible to assay the material on a transfer membrane with differentantigens or antibody probes simultaneously without cutting the membrane intostrips. The membrane remains intact to preserve the correspondence betweendifferent lanes or positions on the gel.

The HOEFER PR 648 SLOT BLOT MANIFOLD provides a simple, convenient methodto immobilize unfractionated samples to a membrane, allowing screening ofrecombinant clones, DNA, RNA and proteins.

PR 648SLOT BLOTBLOTTING MANIFOLD

SEE PAGE 33

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32Immunoblotting


Includes:Top Plate, 45 ChannelsBottom PlateScrews - 2 pcsWashing Manifold - 2 pcsTubing - 2 pcsLuer Manifold Connectors - 4 pcsSealing Pads - 5 pcs

ImmunoBlot XLItem# PR645


Channel Dimensions13 cm long, spread out over 13 cm width;accommodates SE 600 and SE 400 gels.

Operating ConditionsMaximum Temperature: 45 °C

CapacityImmunoBlot XL: 45 channels, 140 µl per channelImmunoBlot: 25 channels, 250 µl per channel


ImmunoBlot & ImmunoBlot XL

Blotting Manifolds

The ImmunoBlot XL has 45 sample channels. Each channel holds up to140 µl of antibody or probe solution.

ImmunoBlot blotting systems are simple and convenient tools for allimmunoblotting techniques like Western blotting, antibody screening and DNAhybridizations.

The ImmunoBlot has 25 sample channels. Each channel holds up to250 µl. Greater volume per channel enables high sensitivity detection oflow affinity and low titer antibodies.

Results appear on a single membrane for convenient side-by-sidecomparison.

Optional washing manifold enables simultaneous washing of allchannels.

Can be used with all conventional detection methods includingenzymatic, radioactive and chemiluminescence.

Optional gel combs precisely line up electrophoresis lanes withImmunoBlot channels.

Includes:Top Plate, 25 ChannelsBottom PlateScrews - 2 pcsWashing Manifold - 2 pcsTubing - 2 pcsLuer Manifold Connectors - 4 pcsSealing Pads - 5 pcs

ImmunoBlotItem# PR625

COMBS

Item#PR511-9-1.0PR511-12-1.0PR511-25-1.0

Thickness# ofWells

1.0 mm1.01.0

91225

Sealing Pads – 10 pcs, Item# PR630-31

Washing Manifold Kit, Item# PR630-32 Includes: Washing Manifold - 2 pcs Tubing - 2 pcs Luer Manifold Connectors - 4 pcs

O-Ring Seals – 2 pcs, Item# PR630-33

Luer Manifold Connectors – 4 pcs, Item# PR630-34

Clamp Screw, Item# PR630-36

Top Plate, for PR 645, Item# PR645T

Top Plate, for PR 625, Item# PR625T

Bottom Plate, for either unit, Item# PR630-37

ImmunoBlot XLImmunoBlotImmunoBlot

Use with


800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

IMM

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33 Immunoblotting

Incubation Manifold

Individually isolated liquid-tight chambers allow you to use differentdetection methods or antibodies side-by-side.

The HOEFER PR 150 DECA-PROBE INCUBATION MANIFOLD has 10incubation chambers for screening antibody preparations or probedilutions. The Deca-Probe has all of the features of the ImmunoBlot ina smaller format. The Deca-Probe is excellent for antibody screeningand Western blotting of minigels.

Screen 10 samples individually on a single 8.8 x 8.8 cm transfermembrane for side-by-side lane comparisons.

Screen up to 10 different antibody preparations against a singleantigen mixture or one antibody preparation against as many as10 different antigen mixtures on a single, intact membrane.

Economical to use. Requires minimal volume of reagents.


Includes:Pressure PlateSample Plate w/10 Sample ChambersTeflon Screws – 6 pcsSample GasketPressure Gasket

Deca-Probe Incubation ManifoldItem# PR150

Reliable. No worry about artifacts caused by incompleteexposure or mixing.


Channel Dimensions:8.8 cm long, spread out over 8.8cm width; accommodates SE 260and miniVE gels.

Operating Conditions:Maximum Temperature: 45 °C

Capacity:10 channels, 0.5 – 2.0 ml per channel


PR 648 Slot Blot

Blotting ManifoldScreens as many as 48 samplesquickly and easily.

The HOEFER SLOT BLOT MANIFOLDoffers a simple, convenient method forscreening recombinant clones, DNA,RNA and proteins.

Each well holds up to 1 ml samplevolume. Slot dimensions are 6 x 0.8 mm.

Numbered slots facilitate accuratesample application.

A raised lip around each slot createsan effective seal and prevents cross-contamination between wells.


Includes:Top BlockMembrane Support Block w/ O-ringBottom Block w/ ConnectorQuickFit Connector w/ O-ringClamping Screws – 6 pcsMembrane Cutting Template

Slot Blot ManifoldItem# PR648

Alignment pins ensure quick andeasy assembly.

Attaches easily to a vacuum pump orwater aspirator.


Slot Dimensions: 6.0 x 0.8 mm; numbered


Capacity: 1 ml sample volume per slot

Unit Dimensions (W x H x D):15.5 x 12 x 8 cm (6.1" x 4.7" x 3.1")

Membrane Size: 11.5 x 8 cm

Teflon Screws – 4 pcsItem# PR153

COMBS

Item#SE211A-10-.75SE211A-10-1.0SE211A-10-1.5

Thickness# ofWells

0.75 mm1.01.5

101010


Pressure GasketItem# PR152

Sample GasketItem# PR151

PR 150 Deca-Probe

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

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34Blotting Membranes

Blotting Membranesfor Western, Northern and Southern Blotting

Hoefer introduces high quality membranes in convenient formats for your blotting needs.

Ideal for Western Blotting and other Immunoblotting techniques.

Available in 0.2 µm and 0.45 µm thickness in rolls and convenient pre-cut sheets.

High wick rates (lateral flow). Substances move quickly through the membrane.

BSA protein binding capacity of 160 µg/cm2. Will bind a wide range of fragment sizes and can still be easily blocked for low background.


Nitrocellulose membrane, 0.22 µm pore size, 30 cm x 3 m rollItem# GM-NC22

Nitrocellulose membrane, 0.45 µm pore size, 30 cm x 3 m rollItem# GM-NC45

Nitrocellulose membrane, 0.45 µm pore size 8 x 9.5 cm, 10/packItem# GM-NC45-89

Nitrocellulose membrane, 0.45 µm pore size 16 x 16 cm , 10/packItem# GM-NC45-1616

Nitrocellulose membrane, 0.45 µm pore size 23.5 x 20 cm, 10/packItem# GM-NC45-2320

Nylon membrane, 0.45 µm pore size, 30 cm x 3 m rollItem# GM-NY45

Nylon membrane, 0.45 µm pore size, 15 x 20 cm, 10/packItem# GM-NY45-1520

PVDF membrane, 0.45 µm pore size, 30 cm x 3 m rollItem# GM-PV45

Nitrocellulose Membranes:Nitrocellulose Membranes:

Superior strength. Withstands aggressive handling or can be used with automated equipment without breaking or tearing.

Uniform pore structure provides consistent flow and diffusion rates.

Stringent quality tests ensure lot-to-lot consistency and produce dependable results every time.

Ideal for Northern and Southern Blotting.

Available in 0.45 µm pore size in rolls and convenient pre-cut sheets.

Inherently charged. Enhances nucleic acid binding–even under alkaline conditions.

Multiple reprobings possible.

Nylon Membranes:Nylon Membranes:

Greater binding capacity, 450 µg/cm2. Can bind a wide range of fragment sizes and still be easily blocked for low background.

Supported. Has added strength and durability, preventing distortion or contamination in multiple reprobings.

Excellent signal. Retains DNA better, resulting in a strong signal using smaller quantities of DNA.

Stringent quality tests ensure lot-to-lot consistency and produce dependable results every time.

Ideal for use in protein binding applications such as western blots,solid phase assays and immunoblotting procedures.

Superior strength. Can withstand aggressive handling or be used withautomated equipment without breaking or tearing.

Available in 0.45 µm pore size in a 3 meter roll.

PVDF (Polyvinylidene Fluoride) Membranes:PVDF (Polyvinylidene Fluoride) Membranes:

Exceptional sensitivity detects low-level components.

Low extractables. Ensures tests will be clean with consistent results.

Hydrophobic. Resists water.

High binding capacity. Binds a wide range of fragment sizes.

Lot-to-lot consistency. Quality checks ensure consistent binding fordependable results every time.

35 Filtration

FILT

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Ten-Place Filtration Manifold

Holds ten individual stainless steel chimney weights, eachwith a capacity of 13 ml.

The HOEFER FH 225V TEN-PLACE FILTRATION MANIFOLD holdsten 25-mm diameter filters in place with simple chimney weights.The manifold rests on a clear, chemical- resistant PVC collectionbox. The Filtration Manifold is recommended for detection ofpathogens like Giardia in water analysis.

Precision-machined chimney weights, made of type 316solid stainless steel, provide a secure, liquid-tight seal.No need for clamps or O-rings.

Clear PVC collection box acts as a vacuum chamber,exerting equal vacuum on all ten positions.

With the optional FH 240 rack, individual filtrates can alsobe collected into test tubes or 27-mm scintillation vials.


Includes:Filter Holding Manifold w/Vacuum Gauge10 Individual Teflon ValvesPVC Collection Box w/GasketTubing ConnectorStainless Steel Chimney Weights – 10 pcs

Ten-Place Filtration ManifoldItem# FH225V

Individual on/off valves allow you to regulate eachposition separately.

PVC collection box is resistant to trichloroacetic acid (TCA).

PVC Collection Boxw/Gasket & Tubing ConnectorItem# FH251

Gasket, 100 cmItem# FH2208

Filter Holding Manifoldw/Vacuum Gauge & ValvesItem# FH225VM


Sample Volume: 13 ml


Filter Size: 25 mm diameter circles

Unit Dimensions (W x H x D):32.4 x 20.3 x 15.2 cm (12.8" x 8" x 6")

Chimney WeightItem# FH252-25


800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

Refrigerated & Heated Circulation Bath

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36Circulation Bath

RCB-20

Provides temperature control in the -20 to +100 °C range, regulated to ±0.1 °C.

The HOEFER RCB-20 REFRIGERATED/HEATED CIRCULATION BATH provides efficienttemperature control for all types of electrophoresis and transfer techniques.

Compact footprint. The RCB-20 is just 20 cm wide x 36 cm deep.

Cooling capacity of 200 W at 20 °C.

Delivers heat with a 1000 W heater (115 VAC).

Designed for continuous use, the RCB-20 has a 15 liter per minute circulation pump.

Appropriate for use with these other Hoefer instruments: the SE 250, SE 260,Chroma, SE 600 and SE 660 vertical electrophoresis units; and the TE 22, TE 42and TE 62 tank transfer units.

Each Includes:Refrigerated/Heated Circulation BathTygon Tubing, 3/8” ID – two 3-foot lengthsQuickFit Connectors – 2 setsPump Connector Barbed Fittings – 2 pcs

RCB-20 Refrigerated & Heated Circulation Bath115 VAC, 60 Hz - Item# RCB20-115V230 VAC, 50 Hz - Item# RCB20-230V100 VAC, 60 Hz - Item# RCB20-100V


Accessories & Spare PartsTubing Kit - Item# RCB21(Tygon Tubing, 3/8” ID – two 3-foot lengths and QuickFit Connectors – 2 sets)


Heater Wattage: 1000 W at 115 V, 2000 W at 230 V

Resolution: 0.1 °C

Cooling Capacity (ethanol medium) (W): 200 120 70 20at temperature (°C): +20 0 -10 -20

Operating ConditionsEnvironmental Operating Conditions:Indoor Use: 5 - 40 °CHumidity: up to 80% for temperatures up to 31 °C; linear decrease to 50% at 40 °CAltitude: up to 2000 m

Refrigerant: R134a

Unit Dimensions (W x H x D):20 x 56 x 36 cm (7.9" x 22" x 14.2")

Working Temperature Range: -20 to +100 °C

QUALITY CONTROL

Hoefer is passionate about electrophoresis,and has been a pioneering force since theearly 1970’s. That dedication to assistinglife scientists begins long before ourinstruments reach your laboratory.

We put every component of a Hoeferdesigned and manufactured productthrough the highest standards in qualitycontrol to ensure that the finished productis built to last and last.

You can be assured our products are thebest that you can buy.

Safety Certifications: EN61010-1, CE

Weight: 22 kg

PowerSupplies37

PO

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Power Supply

Maximum output: 600 V, 500 mA, 150 W.

The EV 265 CONSORT POWER SUPPLY is ideal for electrophoresis and semi-dry blottingtechniques. The electrical leads from all HOEFER gel and blot units are fully compatiblewithout the need for adapters.

Constant voltage, constant current, or constant power mode with automatic crossover.

Programmable step function. Stores and recalls as many as 9 sets of frequently usedparameters.

Timer or volt-hour controlled with alarm.

Automatic recovery after power failure.

Precise, reproducible settings and readouts in single-unit increments(1 V, 1 mA, 1 W, and 1 min).

Manual programming mode allows temporary changes to parameters withoutinterrupting the run.

Integral data-logger periodically stores output values including program numberand step for up to 3600 points.


EV 265 Consort Power Supply, 115 VACItem# PS265-115V


Voltage: 600 V

Timer: 99:59 h

Current: 500 mA

Power: 150 W

Parameter Range: 1 - 100% of full scale

EV 265 Consort Power Supply, 230 VACItem# PS265-230V

Programs: 9 x 9 set of parameters

Volt-Hours: 0 - 99.99 kVh

Display: LCD, 2 x 16 characters

Resolution: 1 V, 1 mA, 1 W

No Load Detection: programmable

Outputs: 4 in parallel, 4 mm sockets

Min. Load Resistance: 30 ohm

Ground Leakage Detection: 500 µA

Overload Detection: Yes

Weight: 5 kg

Data-Logging: 3600 values

RS232: 9600 b/s

Dimensions (W x D x H): 31 x 26 x 15 cm

Safety Certification: CE

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)


PS 2A200 Power Supply,90-265 VAC, 47-63 HzItem# PS2A200


Maximum Output:200 V, 2000 mA, 200 W, automatic crossover

Operating Conditions:Environmental Operating Conditions:Indoor Use: 5 - 40 °CHumidity: 0 - 90% non-condensing

Safety Certifications:EN61010-1, UL 3101-1, CSA22.2 1010-1, CE


Timer: 0 – 99:59 hr or 1 – 9999 Vhr

Output: 2 pairs of sockets, in parallel, 4-mm, HV, recessed

Open Circuit Detection:Open circuit protection for resistanceover 300K Ohms

38PowerSupplies

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Maximum output: 200 V, 2000 mA, 200 W.

The high current PS 2A200 POWER SUPPLY is ideal forall types of electrophoresis blotting applications andfor short electrophoresis runs.

Constant voltage, constant current, or constant powermode with automatic crossover.

Programmable step function. Stores andrecalls 3 protocols.

Precise, reproducible settings and readouts insingle-unit increments (1 V, 1 mA, 1 W, and 1 min).

Prints a log of run parameters through an RS-232 port.

Designed for high current applications such astank blotting.

Choose from 4 timer options:1) Continuous run2) Set time run3) Set time run followed by a hold at 5 volts4) Set volt-hour run.

Automatic recovery after power failure.

Compact design saves bench space.

PS 2A200Power Supply

Weight: 2.2 kg

Gel Casting Reagents

1.1

GEL CASTING KITS SEE PAGE 24

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39 Electrophoresis Reagents

Caliber™

High Quality Electrophoresis Reagents

A complete range of chemicals for electrophoresis.

Hoefer Caliber Electrophoresis Reagents deliverconsistently outstanding results. A high standardof purity, rigorous analytical procedures, andcarefully controlled manufacturing techniquesensure that Hoefer Caliber reagents are the bestquality buffer, gel casting, gel staining and samplepreparation chemicals available.

Free from impurities that interfere withpolymerization.

Manufactured to stringent specifications.

Pure powder or premixed ready-to-use solutions,to meet your lab’s needs and convenience.

Functionally tested by batch to ensurereproducibility.

AcrylamideMB GradeCH2=CHCONH2

Formula weight: 71.08Product Specifications

Form: White/clear crystalline powderAssay: �99.9%Identity: by IRA (410 nm, 35%, 1 cm): �0.02A (290 nm, 1%, 1 cm): �0.1A (280 nm, 7 mM, 1 cm): �0.04A (252 nm, 7 mM, 1 cm): �1.20Insoluble matter (H2O): �0.005%Insoluble matter (MeOH): �0.005%pH (10%, 0.1 N NaCl): 6.0 – 7.0Melting Point: 84 ± 1 °CAcrylic Acid: �0.001%Conductivity (35%): �2 µmhos/cmIron (Fe): �1 ppmLead (Pb): �1 ppmGel Performance: Passes testCAS # 79-06-1

Use: PAGEQuantity: 1 kgItem# GR141-1

N,N'-Methylene-bis-Acrylamide(bis-Acrylamide)MB Grade(CH2=CHCONH)2CH2

Formula Weight: 154.17Product Specifications

Form: White crystalline powderAssay: �99.9%Identity: By IRA (290 nm, 1%, 1 cm): �0.2pH (1%, H2O): 5.5Acrylic acid: �0.02%Lead (Pb): �1 ppmGel Performance: Passes testCAS # 110-26-9

Application: Cross-linking agent used informing polyacrylamide gels. The porosityof the gel is determined in part by thedegree of cross-linking. Most protein gelsuse a ratio of 0.9 g of cross-linker to30 g of acrylamide.Use: PAGEQuantity: 100 gItem# GR142-100

N,N,N',N'-Tetramethylethylenediamine(TEMED)(CH3)2NCH2CH2N(CH3)2


Form: Colorless to pale yellow liquidAssay: �98%A (290 nm, 1%, 1 cm): �0.1Density (20 °C): 0.77 ± 0.01Refractive Index (20 °C): 1.419 ± 0.001CAS # 110-18-9

Application: Catalyzes the formation offree radicals by ammonium persulfate andaccelerates the polymerization of acrylamideand bisacrylamide.Use: PAGEQuantity: 25 gItem# GR151-25

Ammonium Persulfate(APS)ACS Reagent Grade(NH4)2S2O8


Form: Off-white crystalline powderAssay: �98.0%Residue on Ignition: �0.05%Insoluble Matter: �0.005%Titratable Free Acid: �0.04 meq/gChloride (Cl): �0.001%Heavy Metals (Pb): �0.005%Iron (Fe): �0.001%Manganese (Mn): �0.5 ppmSolubility (2 M, H20): Complete, clear and colorlessCAS # 7727-54-0

Application:A high speed initiator used in polyacrylamidegel electrophoresis.Use: PAGEQuantity: 10 gItem# GR152-10

AgaroseMB GradeProduct Specifications

Form: Granular, free-flowing powderEEO (-mr): 0.05 - 0.13Gel Point (1.5%): 36.0 � 1.5 °CRemelt Point (1.5%): 88.0 � 1.5 °CGel Strength (1%): �1200 gm/cm2

Gel Strength (1.5%): �2500 gm/cm2

Moisture: �7%Turbidity (1.5%): �40 NpResidue on Ignition: �0.4%Sulfate (SO4): �0.14%CAS # 9012-36-6

Genetic Performance Tests:DNase: Passes testRNase: Passes testRestriction Enzyme Digestion: Passes testLigation: Passes testDNA Binding: Passes testBlotting: Passes testGel Background: Passes testDNA Resolution: Passes test

Separation Guidelines:

Applications:1. Separation of DNA or RNA fragments.2. PCR analysis.3. Recovery of separated DNA or RNA

fragments from gel.4. Blotting applications such as Southerns

and Northerns.5. Protein analysis such as immunodiffusion,

rocket assays and immunoelectrophoresis.Use: DNA/RNA detectionQuantity: 500 gItem# GR140-500

0.60.81.01.21.52.0

% Agarose

20,000 - 1,00012,000 - 5008,000 - 3006,000 - 2003,500 - 1002,000 - 50

in 1X TAE

15,000 - 1,00010,000 - 5007,000 - 2505,000 - 2003,000 - 1002,000 - 50

in 1X TBESeparation Range (bp)

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Protein Determination ReagentDescription: One bottle of Protein DeterminationReagent is sufficient for 500 standard assays or2500 microassays.Application: This protein dye reagent allowsrapid and simple determination of the proteincontent of various solutions. This reagent isbased on the Bradford(1,2) protein assay methodand is supplied as a five-fold concentrate.It can be used either in a standard assay procedurefor protein concentrations between 100 µg/mland 1 mg/ml or in a microassay procedure forconcentrations between 10 µg/ml and 100 µg/ml.The assay procedure consists of mixing thereagent with sample and blank, incubating (usually5 - 20 min), and measuring the absorbance ofthe sample and blank at 595 nm. The assayprocedure is supplied with every bottle.References:1. BRADFORD, M. M. (1976) Anal. Biochem. 72, 248 - 254.2. BRADFORD, M. M. AND W. L. WILLIAMS (1977) U.S. Patent No. 4,023,933.Use: PAGE, IEF, 2DQuantity: 500 mlItem# GR133-500

Sodium Dodecyl Sulfate (SDS)CH3(CH2)11OSO3NaFormula Weight: 288.38Product Specifications

Form: White crystalline flakesIdentity: by IRAssay: �95% (dry basis)Solubility (20%, H2O): Clear, faintly yellow solutionCAS # 151-21-3

Use: PAGE, blottingQuantity: 500 gItem# GR126-500

Sodium Dodecyl Sulfate (SDS),10% SolutionDescription: 10% (w/v) solution of sodiumdodecyl sulfate (SDS) in high purity dH2O.Solution is 0.2 µm filtered and dispensedinto durable, square Nalgene bottles.Product Specifications

A (230 nm, 3%, 1 cm): � 0.2A (280 nm, 3%, 1 cm): � 0.1

Storage: AmbientNote: Precipitation of SDS is not unusual.Warm gently to redissolve.Use: PAGE, blottingQuantity:1 LItem# GR155-1

Dithiothreitol,(DTT; Cleland's Reagent)MB GradeC4H10O2S2


Form: White crystalline powderAssay (SH): �99.5%A (280 nm, 0.1 M, 1 cm): �0.08A (260 nm, 0.1 M, 1 cm): �0.40Melting Point: 40 – 43 °COxidized DTT: �0.3%DNase: Passes testRNase: Passes testProtease: Passes testCAS # 3483-12-3

Storage: -20 °CUse: PAGE, 2DQuantity: 5 gItem# GR122-5

EDTA, 0.5 M SolutionMB GradeDescription: 0.5 M solution of EDTA-Na2 in high purity dH2O, pH 8.0. Solutionis 0.2 µm filtered and dispensed intodurable, square Nalgene bottles.pH: 8.0 � 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testStorage: AmbientApplication: Chelating agent used inmany enzyme buffers and, at higherconcentrations, as an enzyme inactivator.Use: PAGE, DNA/RNA detectionQuantity: 100 mlItem# GR123-100

Bromophenol Blue,Sodium SaltACS Reagent GradeC19H9Br4NaO5SFormula Weight: 691.95Product Specifications

Form: PowderClarity of Solution: Passes testVisual Transition Interval: pH 3.0 - Yellow pH 4.6 - BlueCAS # 62625-28-9

Applications:Used as a tracking dye in agarose andacrylamide gel electrophoresis. Used inthe stop solution in DNA sequencing kits.Use: PAGE, DNA/RNA detectionQuantity: 10 gItem# GR120-10

GlycerolMB GradeHOCH2CH(OH)CH2OHFormula Weight: 92.09Product Specifications

Form: Clear, colorless, viscous liquidAssay: �99.5%Identity: by IRSpecific Gravity (25 °C): 1.260 – 1.263A (230 nm, 100%, 1 cm): �0.15A (260 nm, 100%, 1 cm): �0.06A (280 nm, 100%, 1 cm): �0.05Color (APHA): �10Neutrality: Passes testResidue on Ignition: �0.005%Moisture: �0.5%Acrolein, Glucose and Ammonium Compounds: Passes testFatty Acid Esters: �0.2 meq/100 gmSilver Reducing Substances: Passes testReadily Carbonizable Substances: Passes testChlorinated Compounds: �0.003%Chloride (CI): �0.001%Sulfate (SO4): �0.002%Arsenic (As): �1.5 ppmCalcium (Ca): �5 ppmIron (Fe): �5 ppmMagnesium (Mg): �1 ppmMercury (Hg): �10 ppmZinc (Zn): �2 ppmHeavy Metals (Pb): �2 ppmDNase (endo): Passes testDNase (exo): Passes testRNase: Passes testCAS # 56-81-5

Use: PAGE, density agentQuantity: 1 LItem# GR124-1

SucroseMB GradeC12H22O11


Form: White/clear crystalline powder[�]D

25: +66.3° to +66.8° (c=26 in H2O)A (260 nm, 1 M, 1 cm): �0.150A (280 nm, 1 M, 1 cm): �0.125Insoluble Matter: �0.005%Loss on Drying: �0.03%Residue on Ignition: �0.01%Titratable Acid: �0.0008 meq/gmInvert Sugar: �0.05%Chloride (Cl): �0.005%Sulfate and Sulfite (as SO4): �0.005%Arsenic (As): �1 ppmBarium (Ba): �1 ppmCadmium (Cd): �1 ppmCalcium (Ca): �5 ppmCopper (Cu): �1 ppmIron (Fe): �1 ppmLead (Pb): �1 ppmMagnesium (Mg): �1 ppmManganese (Mn): �1 ppmZinc (Zn): �1 ppmDNase (endo): Passes testDNase (exo): Passes testRNase: Passes testCAS # 57-50-1

Use: PAGE, density agentQuantity: 1 kgItem# GR129-1

CHAPS(3-[(3-Cholamidopropyl)-Dimethylammonio]-1-Propane Sulfonate)C32H58N2O7SFormula Weight: 614.88Product Specifications

Form: White crystalline powderIdentity: by IRAssay (N analysis): �98%Chromatography (TLC): HomogeneousCritical Micelle Concentration (CMC): 4 to 8 mMCAS # 75621-03-3

Storage: 4 °C, protected from lightUse: PAGE, IEF, 2DQuantity: 10 gItem# GR121-10

UreaMB GradeNH2CONH2


Form: White crystalline powderAssay: �99.5%Identity: by IRSpectral Ratios(260 nm, 6 M, 1 cm): �0.055Spectral Ratios(280 nm, 6 M, 1 cm): �0.044Melting Point: 132 to 135 °CInsoluble Matter: �0.01%Residue on Ignition: �0.01%Conductivity: �15 µmhos/cmCyanate: None detectedChloride (Cl): �0.0005%Sulfate (SO4): �0.001%Copper (Cu): �0.5 ppmIron (Fe): �0.5 ppmLead (Pb): �0.5 ppmDNase (endo): Passes testRNase: Passes testProtease: Passes testCAS # 57-13-6

Use: IEF, 2DQuantity: 1 kgItem# GR143-1

Thiourea (Thiocarbamide)NH2CSNH2


Form: White to off-white crystalline powderIdentity: by IRMelting Point: 171 – 184 °CLoss on Drying: �0.5%CAS # 62-56-6

Use: IEF, 2DQuantity: 500 gItem# GR130-500

Mineral OilMB GradeProduct Specifications

Form: Clear, colorless oilIdentity: by IRSpecific Gravity (25 °C): 0.818 to 0.880Viscosity (40 °C): �33.5 centistokesNeutrality: Passes testReadily Carbonizable Substances: Passes testLimit of Polynuclear Compounds: Passes testSolid Paraffin: Passes testUse Test: Functionally tested for use in PCRCAS # 8042-47-5

Use: IEF, 2D, PCRQuantity: 1 LItem# GR138-1

Sample Preparation And Loading Reagents

41 Electrophoresis Reagents

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Tris-Glycine-SDS Buffer,10X SolutionMB GradeDescription: 10X Tris-Glycine-SDS Buffer,consisting of 0.25 M Tris, 1.92 M glycine and1.0% SDS in high purity dH2O, pH 8.3. Solutionis 0.2 µm filtered and dispensed into durable,square Nalgene bottles. For PAGE, dilute to 1Xwith high purity dH2O for a solution consistingof 25 mM Tris, pH 8.3, 192 mM glycine and0.1% SDS.pH (25 °C, 10X): 8.3 � 0.1Contaminant Testing: Protease: Passes test.Storage: AmbientApplication: TGS is the most commonly usedbuffer for SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) of proteins.Use: PAGE, blottingQuantity: 1 LItem# GR149-1

Tris[Tris(hydroxymethyl)aminomethane]Reagent GradeNH2C(CH2OH)3


Form: White to pale yellow crystalline powderAssay: �98.0% (dry basis)Identity: by IRpH (5%, H2O): 10.0 - 11.5Melting Point: �160 °CLoss On Drying: �1.5%CAS # 77-86-1

Use: PAGE, blottingQuantity: 1 kgItem# GR132-1

GlycineH2NCH2CO2HFormula Weight: 75.07Product Specifications

Form: White crystalline powderAssay: 98.5 to 101.5% (dry basis)Identity: by IRLoss on Drying: �0.2%Residue on Ignition: �0.1%Sulfate (SO4): �65 ppmHeavy Metals (Pb): �20 ppmChloride (Cl): �70 ppmHydrolyzable Substances: Passes testO.V.I.: Passes testCAS # 56-40-6

Use: PAGE, blottingQuantity: 1 kgItem# GR125-1

Tricine[N-Tris (Hydroxymethyl)methylglycine](CH2OH)3CNHCH2COOHFormula Weight: 179.17Product SpecificationsForm: White/clear crystalline powderAssay: �98.5% (dry basis)Identity: by IRpKa (20 °C): 8.15 � 0.15A (250 nm, 5%, 1 cm): �0.1Loss On Drying: �0.5%Iron (Fe): �30 ppmLead (Pb): �10 ppmCAS # 5704-04-1Use: PAGEQuantity: 1 kgItem# GR131-1

TE Buffer, 50X SolutionMB GradeDescription: 50X TE solution consistingof 500 mM Tris, 50 mM EDTA in highpurity dH2O, adjusted to pH 7.5. Solutionis 0.2 µm filtered and dispensed intodurable, square Nalgene bottles. Diluteto 1X with high purity dH2O for a solutionconsisting of 10 mM Tris, pH 7.5, and1 mM EDTA.pH (25 °C, 50X): 7.5 � 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes testStorage: AmbientUse: DNA/RNA detectionQuantity: 100 mlItem# GR144-100

PBS, 10X Solution, pH 7.4MB GradeDescription: 10X PBS (Phosphate-Buffered Saline) solution consistingof 80 mM sodium phosphate, 15 mMpotassium phosphate, 27 mM KCland 1.37 M NaCl in high purity dH2O,pH 7.4. Solution is 0.2 µm filteredand dispensed into durable, squareNalgene bottles. Dilute to 1X withhigh purity dH2O for a solutionconsisting of 9.5 mM phosphate, pH7.4, 2.7 mM potassium chloride and137 mM NaCl.pH (25 °C, 10X): 7.4 � 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes testStorage: AmbientUse: Sample prep,immunodetection, cell culture,purificationQuantity: 1 LItem# GR145-1

Boric AcidMB GradeH3BO3


Form: White crystalline powder.Assay: �99.5%Insoluble in MeOH: �0.005%Nonvolatile with MeOH: �0.05%Sulfate (SO4): �0.01%Heavy Metals (Pb): �0.001%Arsenic (As): �1 ppmCalcium (Ca): �0.005%Chloride (Cl): �0.001%Iron (Fe): �0.001%Phosphate (PO4): �0.001%Functional Test: Meets or exceedscriteria for high quality sequencinggel electrophoresis with SequenaseT7 DNA Polymerase.DNase (endo): Passes testDNase (exo): Passes testRNase: Passes testProtease: Passes testCAS # 10043-35-3

Use: DNA/RNA detectionQuantity: 1 kgItem# GR153-1

TBE Buffer, 10X Ready-Mixed PowderMB GradeDescription: Pre-weighed, pre-mixedpowdered buffer packaged in 200ml bottles for reconstitution to a10X solution. Reconstitute with highpurity dH2O to 200 ml. Afterreconstitution, the 10X solutionconsists of 0.89 M Tris, 0.89 Mboric acid and 0.02 M EDTA. Eachpack contains 6 x 200 ml bottles.Total volume is 1.2 liters of 10Xbuffer or 12 liters of 1X buffer.Testing: Meets or exceeds functionaltest criteria for high quality gelelectrophoresis.Storage: Ambient. The reconstitutedbuffer is stable for several days at4 °C if 0.2 µm filtered.Use: DNA/RNA detectionQuantity: 6 x 200 ml after reconstitutionItem# GR136-6X200

TBE Buffer, 5X SolutionMB GradeDescription: 5X TBE (Tris-Borate-EDTA) solution consisting of 0.445M Tris, 0.445 M boric acid and0.01 M EDTA. Solution is 0.2 µmfiltered and dispensed into durable,square Nalgene bottles. Dilute to1X with high purity water for asolution consisting of 0.089 M Tris,0.089 M boric acid and 0.002 MEDTA.Testing: Meets or exceeds functionaltest criteria for high qualitysequencing gel electrophoresis.Storage: AmbientUse: DNA/RNA detection, SequencingQuantity: 1 LItem# GR146-1

Tris-Glycine Buffer, 10X SolutionMB GradeDescription: 10X Tris-Glycine Buffer consistingof 0.25 M Tris and 1.92 M glycine in high puritydH20, pH 8.3. Solution is 0.2 µm filtered anddispensed into durable, square Nalgene bottles.For blotting dilute to 1X with high purity waterand methanol for a solution consisting of 25 mMTris, pH 8.3, 192 mM glycine, 20% methanol.pH (25 °C, 10X): 8.3 � 0.1Contaminant Testing: Protease: Passes test.Storage: AmbientUse: PAGE, blottingQuantity: 1 LItem# GR148-1

Buffer and Buffer Preparation Reagents

42Electrophoresis Reagents

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TSTAE Buffer, 10X SolutionMB GradeDescription: 10X TAE solutionconsisting of 400 mM Tris and 0.01M EDTA in high purity dH2O adjustedto pH 8.3. Solution is 0.2 µm filteredand dispensed into durable, squareNalgene bottles. Dilute to 1X with highpurity dH2O for a solution consistingof 40 mM Tris Acetate, pH 8.3 and1.0 mM EDTA.pH (25 °C, 10X): 8.3 � 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testStorage: AmbientUse: DNA/RNA detectionQuantity: 1 LItem# GR150-1

Water, RNase-Free, DEPCMB GradeDescription: High purity dH2O treatedovernight with 0.1% Diethylpyrocarbonate.Product is 0.2 µm filtered and dispensedinto durable, square Nalgene bottles, thenautoclaved.Contaminant Testing: RNase: Passes test DNase (endo): Passes test DNase (exo): Passes test Enzyme Inhibition: Passes testStorage: AmbientUse: DNA/RNA detectionQuantity: 1 LItem# GR137-1

Water, RNase-Free, non-DEPCMB GradeDescription: High purity dH2O testedfor contaminating RNase activity. Productis 0.2 µm filtered and dispensed intodurable, square Nalgene bottles.Contaminant Testing: RNase: Passes test DNase (endo): Passes test DNase (exo): Passes testStorage: AmbientUse: DNA/RNA detectionQuantity: 1 LItem# GR139-1

TBS, 20X Solution, pH 7.4MB GradeDescription: 20X TBS (Tris-BufferedSaline) solution consisting of 500 mMTris, 60 mM KCl and 2.8 M NaCl inhigh purity dH2O, pH 7.4. Solution is0.2 µm filtered and dispensed intodurable, square Nalgene bottles. Diluteto 1X with high purity water for asolution consisting of 25 mM Tris, pH7.4, 3.0 mM KCl and 140 mM NaCl.pH (25 °C, 20X): 7.4 � 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes testStorage: AmbientUse: Western detectionQuantity: 1 LItem# GR147-1

TBST, 20X Solution, pH 7.4MB GradeDescription: 20X TBS (Tris-BufferedSaline) solution consisting of 500 mMTris, 60 mM KCl, 2.8 M NaCl, and1.0% Tween 20 in high purity dH2Oadjusted to pH 7.4. Solution is 0.2 µmfiltered and is supplied in an uprightcarton with a convenient dispensingspout. Dilute to 1X with high puritywater for a solution consisting of 25mM Tris, pH 7.4, 3.0 mM KCl, 140mM NaCl and 0.05% Tween 20.pH (25 °C, 20X): 7.4 � 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test Protease: Passes testStorage: AmbientUse: Western detectionQuantity: 1 LItem# GR154-1

SSC, 20X SolutionMB GradeDescription: 20X SSC solutionconsisting of 0.3 M trisodiumcitrate and 3.0 M sodium chloridein high purity dH2O, pH 7.0.Solution is 0.2 µm filtered anddispensed into durable, squareNalgene bottles.pH (25 °C, 20X): 7.0 � 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testStorage: AmbientUse: Blotting, hybridizationQuantity: 1 LItem# GR127-1

Tween® 20, Ultrapure(Polyoxyethylene SorbitanMonolaurate)Product Specifications

Form: Yellow liquidIdentity: Passes testSaponification Number: 40 – 50 mg KOH/gHydroxyl Number: 96 – 108 mg KOH/gAcid Number: �2.2 mg KOH/gWater: �3.0%Specific Gravity: 1.1 g/ml (approx.) (25 °C)Viscosity: 370 cps (approx.) (25 °C)Solubility (10%, H2O): SolubleCAS # 9005-64-5

Use: Western detectionQuantity: 500 mlItem# GR128-500

Brilliant Blue G(Coomassie® Brilliant Blue G-250; C.I.42655)C47H48N3S2O7NaFormula Weight: 854.02Product Specifications

Form: Dark blue/purple crystalline powderDye content: Approx. 60%Loss On Drying: �8%�max: 607 � 1 nm�max: 38 x 103

CAS # 6104-58-1Application: Used for quantitative staining ofproteins on polyacrylamide electrophoresis gels.Use: Protein stainQuantity: 25 gItem# GR134-25

Brilliant Blue R(Coomassie® Brilliant Blue R-250; C.I.42660)C45H44N3 S2O7NaFormula Weight: 825.97Product Specifications

Form: Dark purple/violet powderIdentity: by IR�max: 586 ± 2 nm�max: �45.0 x 103

Moisture (KF): �6.0%CAS # 6104-59-2

Application: Used for quantitative staining ofproteins on polyacrylamide electrophoresis gels.Use: Protein stainQuantity: 25 gItem# GR135-25

CaliberHigh Quality Electrophoresis Reagents

Gel Staining Reagents

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

43 ULTimaImaging Platform

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Full line of upgradable instruments (ULTima10si, 16si, 16siPlus and Pro): Allows you to upgrade to higher specificationsas research needs change.

The ULTima imaging platformis the fastest, moststraightforward and readilyupgradable unit availableincorporating the latestadvances in electronics andoptics. The four ULTimamodels can economicallyhandle everything from themost routine to the mostdemanding applications inproteomics and genomicswhich have until nowrequired expensive laserscanning systems.

Unique patented image positioning:Makes positioning of gels accurate and quick with amininum of handling of fragile gels.

Slant-correction:Permits the optical rotation of the sample for best ‘straight’visualization without touching the gel.

True multi-fluorophore capabilities:Supports a wide range of applications and isparticularly well suited for proteomics (Cy™-dye imaging)

Super Sensitivity:Performs fast chemiluminescent imaging.Enables faint-band fluorescent detection.

Electronic 5 position emission filter changer:Allows automatic application setup for all imaging applications.

Large image format of 21 x 26 cm:Captures full large-format proteomic gel images in a single pass.

All acquisition parameters stored:Facilitates GLP use.

Smaller footprint with door that slides up:Uses absolute minimum working space and eliminates theneed to step back to open the door.

The optional ULTima Optix illumination module provideshigh intensity excitation of your sample with visible and near-infrared light.

TThe ULTima imaging line has fourhe ULTima imaging line has four

gel documentation systems plusgel documentation systems plus

the optional Optix illuminationthe optional Optix illumination

modulemodule. All models can be. All models can be

upgraded to higher specifiedupgraded to higher specified

versions giving you the flexibility toversions giving you the flexibility to

purchase the model that meetspurchase the model that meets

your needs and budget todayour needs and budget today andy and

upgrade it as your needs increase.upgrade it as your needs increase.

All models come with ULTima AQ All models come with ULTima AQ

control and image acquisitioncontrol and image acquisition

software and ethidium bromidesoftware and ethidium bromide

emission filteremission filter..

See paSee pagges 45 - 46.es 45 - 46.

HoHow to cw to choosehooseyour ULyour ULTTima ?ima ?

44ULTimaImaging Platform

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Slant CorSlant CorrectionrectionSlant correction is a unique feature thatallows the user to turn the imageoptically ± 5 degrees. In this way thesample can be positioned so theanalysis software is better able toperform lane detection.

ImaImagge Pe PositioningositioningUnique image positioning using patented optics. Every user of animaging system has experienced the problem that the gel is never in theright place initially. With most commercially available systems the userhas to open the drawer or door to reposition the gel for the bestvisualization. The ULTima however is the only imaging system withpatented optics for image positioning without loss of optical resolution.This also ensures that lens adjustments are not required. The image ispositioned with the positioning keys without having to open the cabinetor adjust a fragile gel.

Broad Range of ApplicationsBroad Range of ApplicationsThe emission filter changer accommodates a maximum of 5 filters.The filters are selected from the drop-down list or from free definabletemplates. As standard the ULTima is equipped with an emission filterfor the detection of ethidium bromide and other fluorophores within inthe range from 560 to 640 nm (e.g. SYPRO™ Ruby). Filters for mostother fluorophores are available (for example SYBR™ Green II, Cy™3,Cy5, FITC, etc.).

GLPGLPDocumentation of results and the integrity of data becomes muchmore important for drug discovery and genetic engineering.GLP (Good Laboratory Practice) can only be achieved if all imageparameters and conditions are known and archived. ULTima is fullyequipped for GLP validation and verification.

Quantifiable DataQuantifiable DataThe demanding ULTima user can be certain of producing highquantifiable data. The flat fielding function performs a uniformcalibration for every individual image pixel.

Robot ReadyRobot ReadyThe UV 26 transilluminator unit is fully accessible. Samples forautomated 2D analyses can be loaded by robotic arm/system.

Superior Dynamic RangeSuperior Dynamic RangeSince every pixel within the image represents intensity data, it isimportant to achieve the highest possible conversion resolution.In most commercially available systems this is 8, 10 or 12 bit,representing 256, 1024 or 4096 grey levels. The ULTima 16si,ULTima 16si Plus and the ULTima Pro have real 16 bit dynamicranges which means 65536 real different grey levels. This offers muchmore precise quantification but also allows for shorter exposure timesfor chemiluminescent images. The exposure time is normally muchshorter than the exposure time needed for film with comparableimage quality.

TemplatesTemplatesTemplates allow the user to define the settings for image acquisition,including the excitation and emission filters. The parameters arestored per specific application.

User InterfaceUser InterfaceIn order to be able to acquire a high quality image, full control ofthe optics is indispensable. The Image Control screen is alwaysvisible at the bottom of the image, providing ultimate control overthe imaging process. The user has the choice of either setting theexposure time manually, or making use of the fully automaticcapabilities of the ULTima. Full lens control allows optimizationof a broad range of applications.

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

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Includes:UV-26 TransilluminatorULTima AQ communication software suiteTotal Lab TL 100 analysis software

Item# ULT16F

ULTima 16si Plus Imaging Systemplus FILTER SELECTOR

Includes:ULTima AQ communication software suite

Item# ULT161F

ULTima 16si Plus Imaging Unitplus FILTER SELECTOR

16si PlusORDERING INFORMATION

Max. Pixel Size1392 x 1040

16 bit CCD

The ULTima Pro is designed withinnovative 2-stage Peltier cooling of theimage sensor. Rather than attempt to coolthe entire CCD chip to a very lowtemperature, the ULTima Pro cools only theareas of the chip where cooling is essentialto achieve low noise levels at longmeasurement times. This substantiallylengthens the lifetime of the CCD chip andthe camera system. As with ULTima 16siPlus, the ULTima Pro is equipped with anadditional computer-controlled focus andiris control.

ULTima Pro

High sensitivity chemiluminescenceimaging system.

ULTima Pro has the top of the line16 bit temperature controlled cooledCCD camera (65536 gray levels).

Image positioning / Slant correction.

Electronic optical iris.

Electronic optical focus.

Electronic optical zoom (6X).

White light top illumination.

ULTima 10si & 16si

General purpose gel documentationsystems.

Fluorescence ready.

ULTima 10si has a 10 bit CCD camera(1024 gray levels) and ULTima 16si has a16 bit CCD camera (65536 gray levels).




Use ULTima 10si for basic documentationpurposes. With ULTima 16si yourmeasurements are more sensitive andallow more accurate quantification.Both models can be used for imagingethidium bromide stained DNA agarosegels, Coomassie stained gels, SYBR Greenstained gels, SYPRO Ruby stained gels, filmarchiving and petri dishes.

ULTima 16si PlusHigh dynamic range generalpurpose gel documentation system.

ULTima 16si Plus has a 16 bit CCDcamera (65536 gray levels).


Electronic optical iris.

Electronic optical focus.


ULTima 16si Plus is equipped formulti-plexed multi-fluorophoreapplications.



10 bit CCD

10si ORDERING INFORMATION


Item# ULT10

ULTima 10si Imaging System


Item# ULT101ULTima 10si Imaging Unit

16si ORDERING INFORMATION


Item# ULT16

ULTima 16si Imaging System


Item# ULT161ULTima 16si Imaging Unit


16 bit CCD


Item# ULT16CULTima Pro Cooled Imaging System


Item# ULT161CULTima Pro Cooled Imaging Unit

Pro ORDERING INFORMATION


16 bit Cooled CCD

UV-26 Transilluminator (see pg. 49)


Item# UV26

46ULTimaImaging Platform

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ULTima TECHNICAL SPECIFICATIONS

ULTima 16si Plus ULTima ProULTima 16siULTima 10si

Mains: 110/230 Voltage AC automatic detection 50-60 Hz 180 Watt max. Installation Cat. II

For installing and using the ULTima and ULTima AQ software the following computer minimum specifications are required:Computer working under Windows™ 2000, Windows XP-Home (SP1) or XP-Professional (SP1) minimum 256 Mb RAM, minimum screen resolution1024 x 768, one free USB 2.0 port (800 mA or 500 mA) one free RS-232C port (9 pin), pointing device, 1 Gb free harddisk space.

Weight: 41 kg.

Filetype: tiff (16 bit), Tif (8 bit), bmp, jpg, gif

Outer Dimensions (W x H x D): 510 x 380 x 500 mm

Resolution (pixel)

Resolution (sec.)

Image Sensor Cooling

Camera Bit Depth

1312 x 1032

0.001 - 10

No

10

1392 x 1040

0.001 - 10

No

16

1392 x 1040

0.001 - 10

No

16

1392 x 1040

0.001 - 3000

Yes, 6 arrays

16

Electronic Optical Zoom

Electronic Iris Control

Electronic Focus Control

6 x

No

No

6 x

No

No

6 x

Yes

Yes

6 x

Yes

Yes

Image Position System

Slant Control

Yes

± 5 degrees

Yes

± 5 degrees

Yes

± 5 degrees

Yes

± 5 degrees

Emission Filter Positions

White Light Top Illumination

Automatic Door Lock

1 (5 optional)

Yes

No

1 (5 optional)

Yes

No

1 (5 optional)

Yes

No

1 (5 optional)

Yes

Yes

Contrast Control

Brightness Control

Flat Fielding

Saturation Indication

Image Addition (x)

Addition Interval (min.)

Yes

Yes

Yes

Yes

No

No

Yes

Yes

Yes

Yes

1 - 25

0 - 20

Yes

Yes

Yes

Yes

1 - 25

0 - 20

Yes

Yes

Yes

Yes

1 - 25

0 - 20

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

Hoefer offers a choice of software solutions for the analysis of your 1D gelsand blots.

TL 100 - Basic entry level software for fast, consistent analysisTL 120 - For an in-depth and accurate automatic analysis of 1D gels andband pattern matching studies

Please select the software that best meets your needs by identifying yourarea of work below:

TotalLab™

Analysis Software


Molecular Weight CalibrationMolecular Weight CalibrationThe TL 100 and TL 120 versions both enable precise molecular weightdetermination of band material from a variety of 1D gels. TL 120contains tools which allow increased control over calibration forparticularly distorted 1D gel images.

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Item# ULT162

TotalLab TL 100 Analysis SoftwareSingle user license.


Item# ULT163

TotalLab TL 120 Analysis SoftwareSingle user license.

Quantitation and Normalization of Band MaterialQuantitation and Normalization of Band MaterialThe TL 100 and TL 120 software solutions both contain a complete rangeof tools to quantify band material and for the calculation of normalizedband volumes. For in-depth quantitative studies, the advanced features inTL 120 offer a higher degree of flexibility and facilitate accuratequantitation of bands on more problematic 1D gel images.

Lane Relationship StudiesLane Relationship StudiesThe TL 120 software has a powerful band matching facility which isflexible yet easy to use. There are tools available to visualize the resultsof matching and identify similarities within a single gel. When you haveperformed intra gel matching, lanes can be classified and identified.

Summary of features in the TotalLab range:Summary of features in the TotalLab range:TL 100 TL 120

Automatic detection of lanes and bands

Automatic background subtraction

Lane templates

Image manipulation tools

Analysis protocols for batch processing

Molecular weight calibration

Quantity calibration

Normalisation

Profile deconvolution

Rf calibration

Band picking

Band pattern matching - single gel

Dendrogram - single gel

Reports

Array, dot / slot blot analysis

Colony counting

A toolbox for general image analysis

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

Pseudo-color image ofmacro array, labeledwith Cy5.5

GFP detection inleaves.

Applications that are possible with the addition of theOptix module include:CyDye imaging, array detection, in vivo GFPdetection and GFP tissue and organ screening.

OptixPatented Optical Module

DNA remains intact for applications downstream.

The Optix module is a patented epi- illumination optionfor the excitation of fluorophores within the VIS/NIRrange. When Optix is used for ethidium bromide stainedgels, it offers the following advantages:

Lower background means higher detection limits.

Selection of excitation wavelength is fully computercontrolled and, in combination with the automaticemission filter changer, true multi fluorophorecapability is achieved. Multiple images with differentexcitation/emission filter sets allow the detection ofmultiple fluorophores within the same sample.The Optix can accommodate a maximum of fivedifferent excitation filter sets. The Optix module canbe added to all models of the ULTima line.

Optix Illumination Module


Item# ULT164

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Rf calibration accounts for distortionsacross the gel in TL 120.

TL 100 Control Center

ID Gel Image analyzed by TL 100

Analysis protocols enable consistentprocessing of multiple gels in TL 120.

Calibration of a 1D gel using multiplemolecular marker lanes in TL 120.

ULTimaImaging Platform

Shortwave UV lamps are available to change illuminationfrom 302 nm to 254 nm.

MacroVue UV-20 & UV-26

UV Transilluminators

49 Imaging

Uniform illumination.

HOEFER MACROVUE UV TRANSILLUMINATORSare available in two sizes. The active area of theUV-20 is 20 x 20 cm; the active area of the UV-26is 21 x 26 cm.

High (9000 µW/cm2) or low intensityoperation (7000 µW/cm2) for analytical orpreparative applications.

Shortwave UV lamps are available to changeillumination from 302 nm to 254 nm.

UV safety cover minimizes personal exposure.


Ultraviolet Lamps:8 W 302 nm UV dual bi-pin discharge type tubes.UV-26: Six lampsUV-20: Five lamps

UV-20 intensities, approx.:high: 9,000 µW/cm2

low: 7,000 µW/cm2

Safety CertificationsEN61010-1, UL 3101-1, CSA22.2 1010-1, CE

Illuminated Area:UV-26: 21 x 26 cmUV-20: 20 x 20 cm

Unit Dimensions (W x H x D):UV-26: 35.5 x 12.1 x 35 cm (14" x 4.75" x 13.75")UV-20: 33.7 x 12.1 x 24.1 cm (13.25" x 4.75" x 9.5")

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MacroVue UV-20, 115 VACItem# UV20-115V

MacroVue UV-20, 230 VACItem# UV20-230V

MacroVue UV-26, Universal VoltageItem# UV26

MacroVue UVis-20

Versatile. Just push a button to change from one type ofillumination to the other.

The MACROVUE UVIS-20 UV + VISIBLE LIGHTTRANSILLUMINATOR houses both 302 nm midrange UV andvisible (white) light lamps. Each filter is 20 x 20 cm.

Illuminates uniformly.

Dual intensity UV lamps and long-life filters accommodateanalytical and preparative applications.

UV safety cover minimizes personal exposure.

SPECIFICATIONS

Ultraviolet Lamps:UV: Five 8 W 302 nm UVWhite light: Two 8 W visible light

UV intensity, approx.:8,000 µW/cm2

Safety CertificationsEN61010-1, UL 3101-1,CSA22.2 1010-1, CE

Illuminated Area:two 20 x 20 cm surfaces

Unit Dimensions (W x H x D):48.6 x 14.3 x 33.7 cm(19.1" x 5.6" x 13.3")


Each Model Includes:Combination UV + White Light TransilluminatorUV Lamps, 302 nm – 5 Lamps (installed)Cool White Lamps – 2 Lamps (installed)Hinged Clear UV-Blocking Safety Cover

MacroVue UVis-20, 115 VACItem# UVIS-20-115V

MacroVue UVis-20, 230 VACItem# UVIS-20-230V

Each Model Includes:UV Transilluminator w/ Intensity SwitchUV Lamps, 302 nm (installed) - 5 lamps (UV-20) or 6 lamps (UV-26)Hinged Clear UV-Blocking Safety Cover

MacroVue Accessories & Spare PartsUV Lamp, 8 W, 302 nmItem# UVLM-8

UV Lamp, 8 W, 254 nmItem# UVLS-8

Hinged UV-Blocking Safety Cover(for UV-20 or UV-26)Item# UV20RK-1

Hinged UV-Blocking Safety Cover(for UVIS-20)Inquire

Cool White Lamp, 8 WInquire

Combination UV + Visible Light Transilluminator

DQ 300 Fluorometer

DNA/RNA Quantitation

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50Quantitation

Choose either ultraviolet or blue excitation wavelengths.

The DQ 300 FLUOROMETER is a compact, dual-channelunit designed to produce quick, easy, and accuratefluorescence measurements.

Detects DNA accurately in the presence of interferingRNA or protein.

Detects down to 1 ng of DNA or RNA in 1 ml.

Assay low volume samples with the optional Minicell adapter.

Operates in two modes: Discrete (one time 5-secondaverage) or Continuous (user-defined serialmeasurements).

Logs up to 1000 data points in internal memory.

RS-232 cable connects to the serial port of a PC and outputsresults using spreadsheet interface software supplied.


Excitation Range:UV Channel: 365 - 395 nmBlue Light Channel: 465 - 485 nm

Sensitivity:1 ng/mL DNA using PicoGreen®1 ng/mL RNA using RiboGreen®10 ng/mL DNA using Hoechst 33258100 ng/mL protein using NanoOrange™

Safety Certifications:EN61010-1, UL 3101-1,CSA22.2 1010-1, CE

Unit Dimensions (W x H x D):14 x 6.9 x 18.4 cm (5.5" x 2.7" x 7.25")

Emission Range:UV Channel: 440 - 470 nmBlue Light Channel: 515 - 575 nm


Includes:FluorometerPower Supply KitSpreadsheet Interface Software CD-ROMMethacrylate Cuvettes - 4 pcsRS-232 Serial Cable

DQ 300FluorometerItem# DQ300

Includes:Minicell AdapterBorosilicate Glass Tube Cuvettes - 400 pcs

Minicell Adapter Kit(for 50 - 250 µl samples)Item# DQ310

Accessories & Spare PartsStandard Polystyrene Cuvettes - 100 pcs, (10 x 10 mm square, 3.5 ml), Item# DQ305

Standard Methacrylate Cuvettes - 100 pcs, (10 x 10 mm square, 3.5 ml), Item# DQ306

Minicell Borosilicate Glass Tube Cuvettes - 400 pcs, (cylindrical, 250 µl), Item# DQ315

Hoechst 33258 Dye – 100 mg, Item# DQ201

Calf Thymus DNA, Fluorescence Standard – 250 µg, Item# DQ202

4-Methylumbelliferone, Item# DQ203

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

Detection51

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HB-1000Hybridization Oven

Stackable design to maximize laboratory space.

The HB-1000 HYBRIDIZATION OVEN is designed to create theappropriate environment for nucleic acid hybridization.

Temperature control: ambient +10 ºC to 99.9 ºC.

UVC 5000Ultraviolet Crosslinker

The crosslinker can also be stacked on top of the HB-1000Hybridization oven to maximize laboratory space.

The new UVC 5000 UV CROSSLINKER features a pull-out drawerand microprocessor controls to ensure optimal UV irradiation ofDNA. It is also suitable for UV bonding and photonicking of DNA.

Choice of preset and manual controls for energy or time-limitexposures. Preset limits deliver 120,000 µJ or five min ofexposure.

Large LED display and touch keypad.

UV-blocking window allows safe viewing of the process.

Microprocessor measures and controls UV output, ensuringmaximum dose consistency.

Variable speed control (10 to 18 RPM) for consistent wetting ofsamples, for washing or hybridizing.

A large LED displays current chamber temperature. An adjacenttouch pad sets the temperature.

The rotary tube rack can be replaced with a rocker plate fordish-style incubation and washing protocols in one benchtopunit.

A removable protective tray allows easy spill cleanup.

The rotary wheel can hold twenty 35 x 150 mm, or ten35 x 300 mm tubes at one time.

Stainless steel internal construction.

Multiple tube sizes available.

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

52Detection

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UVC 5000 ORDERING INFORMATION

UVC 5000 TECHNICAL SPECIFICATIONS

UV Lamps:Five 8 W 254 nm UV dual bi-pin discharge type tubes.

Maximum Settings:Energy: 999,900 µJ/cm2

Time: 999.9 min


Unit Dimensions (W x H x D):external: 40.0 x 22.2 x 34.9 cm (19.1" x 5.6" x 13.25")chamber: 25.4 x 12.7 x 30.5 cm (10.0" x 5.0" x 12.0")

Features:Microprocessor controlled UV sensor feedback system,large LED readout, multiple set functions (pre-set oruser-specified UV energy or time)

Each Model Includes:UV CrosslinkerUV Lamps, 254 nm – 5 Lamps

UVC 5000 Ultraviolet Crosslinker, 230 VACItem# UVC5000-230V

UVC 5000 Ultraviolet Crosslinker, 115 VACItem# UVC5000-115V

UV Lamp, 8 W, 254 nmItem# UVLS-8


Hybridization Oven &Ultraviolet Crosslinker

stack to maximize lab space!

HB-1000, Hybridization Oven, 230 VACItem# HB1000-230V

HB-1000, Hybridization Oven, 115 VACItem# HB1000-115V

HB-1000 TECHNICAL SPECIFICATIONS

Variable Speed: 10 to 18 RPM

Power: 12V AC/DC from 115V/60Hz or 230V/50Hz

Bottle Capacity:Twenty (20) 35 x 150 mmTen (10) 35 x 300 mm

Temperature Uniformity:Inside Oven: +0.1 °C to 68 °CInside Bottles: +0.1 °C to 68 °C

Heating Element: 1200 Watts

Operating Range: Ambient to +10 °C to 99.9 °C

Temperature Control Accuracy: +0.1 °C

Temperature Display: LED

Temperature Stability:Inside Oven: +0.3 °C to 68 °CInside Bottles: +0.1 °C to 68 °C

Bottle, 35 x 150mm, Item# HB1110XBottle, 35 x 300mm, Item# HB1111XRocker plate, Item# HB1140X


Safety Certification: CE

HB-1000 ORDERING INFORMATION

Detection53

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SE 1200 Easy Breeze™

Air Gel-Drying System



Operating Conditions:Environmental Operating Conditions:Indoor Use: 4 - 40 °CHumidity: up to 80%Altitude: up to 2000 m


Unit Dimensions (W x H x D):32 x 27 x 51 cm (12.5" x 10.5" x 20")

Air circulates above and below the gel frames producing even heating thatdries gels without cracks.

Hoefer’s compact SE 1200 EASY BREEZE AIR GEL-DRYING SYSTEM comescomplete with two gel frames -- everything you need to dry two gels as large as20 x 20 cm, or eight 8 x 10 cm gels.

Drying oven accommodates 6 Hoefer gel frames, allowing you to dry asmany as 6 standard or 24 minigels at a time.

Gel frames are easy to assemble and load. Gels are held taut and smooth.No wrinkling.

No need to monitor the unit. Most gels dry in 2 to 3 hours.

Requires no vacuum pump, cold trap, or bulky connections.

Temperature adjusts from 40 to approximately 50 �C. You can alsodry gels without heat using just the fan.

Includes:Easy Breeze Drying OvenStandard Gel-Drying Frame Assemblies – 2 setsLoading PlatformPorous Cellophane Sheets – 50 pcsMylar Sheets – 25 pcs

SE 1200 Easy Breeze, 230 VACItem# SE1200-230V

SE 1200 Easy Breeze, 115 VACItem# SE1200-115V

Gel-Drying Frame Assemblies:


Standard (up to 20 x 20 cm gel)Item# SE1210

Large (up to 21 x 26 cm gel)Item# SE1210-L

Gel-Loading Platform:

Standard (for SE 1210 gel frame)Item# SE1214

Large (for SE 1210-L gel frame)Item# SE1214-L

Porous Cellophane:

Standard, 33 x 33 cm (50 pcs)Item# SE1202

Large, 35 x 44 cm (50 pcs)Item# SE1142

Mylar:

Standard, 33 x 33 cm (25 pcs)Item# SE1204

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

54Detection

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NGD 2000Vacuum Gel-Drying System

Filter Paper, 35 x 44 cm (25 sheets)Item# SE1141


Heat and vacuum are applied from beneath the gel through aheavy aluminum platen for even heat and vacuum distributionunder the gel.

The Hoefer GD 2000 VACUUM GEL-DRYING SYSTEM uses adry-heat vacuum method to dry gels rapidly, evenly, and safely.

Independent timers for heat and for vacuum.

A quiet, oil-free diaphragm pump maintains the low vacuumnecessary for rapid gel drying.

Thermostat adjusts from 40 to 80 �C, ± 2 �C.


Includes:GD 2000 Gel Dryer w/Silicone Rubber Overlay SheetVP 200 Vacuum PumpStainless Steel ScreenPorous Polyethylene Cover SheetMylar Cover SheetPorous Cellophane – 50 sheetsFilter Paper – 10 sheetsVacuum Tubing – 3 m

GD 2000 Vacuum Gel-Drying System, 115 VACItem# GD2000-115V

GD 2000 Vacuum Gel-Drying System, 230 VACItem# GD2000-230V


Operating Conditions:Environmental Operating Conditions:Indoor Use: 15 - 40 °CHumidity: up to 80% for 15-31 °C,decreasing linearly to 50% for 31-40 °CAltitude: up to 2000 m


Unit Dimensions (W x H x D):55.0 x 8.5 x 43.5 cm (21.6" x 3.4" x 17")

Maximum Operating Ranges:Heater: 800 W, thermostatically controlledPump Outlet: 575 W

Power Requirements:115 VAC, 50/60 Hz, or230 VAC, 50/60 Hz

Porous Cellophane, 35 x 44 cm (50 sheets)Item# SE1142

Clear Silicone Rubber Overlay SheetItem# SE1143

Mylar Cover SheetItem# SE1144

Porous Polyethylene Cover SheetItem# SE1145

Stainless Steel ScreenItem# SE1146

Vacuum Tubing, 8 mm ID, 3 mItem# VT3

Dries acrylamide and agarose gels as large as 33 x 44 cm inas little as 30 minutes.

1.1


Radiation SafetyHoefer Beta and Gamma Radiation Protection Devices

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All products offer effective protection and an unobstructed view.

Protect your lab team from low-level radioactive emissions with Hoefer beta and gammashields, storage boxes, cabinet workstations, and waste receptacles.

Shields, boxes, and bins are available for either beta or gamma radiation protection.

10 mm thick optical acrylic products block beta-emitting isotopes such as 32P, 35S, 3H, and 14C.

12 mm thick lead-acrylic products protect from gamma-emitting isotopes such as123I, 125I, 133Xe, 57Co, 99mTe, and 67Ga.

Hourglass shields provide easy access to the central area behind the shield.

SHIELDS

Rectangular shields have adjustable (0 to 15°) or fixed angles (15° for working in a sittingposition or 45° for standing).

Storage boxes come in three sizes: Mini, Midi, and Maxi. Tube-holding insertsare available for each size.

STORAGE, TRANSPORT, AND DISPOSAL BOXES

Easy to use pipette tip disposal boxes minimize exposure.

Offers all-around protection for operatorsand those working nearby.

BETA RADIATION PROTECTION CABINET

Affords easy access to work areathrough two large hinged doors.

Users have an unobstructed viewwhether sitting or standing.

Bins safely hold low-level radioisotopewaste.

HINGED-LID WASTE BINS AND BAGS

Heavy-duty plastic waste bags fitconveniently inside all bins and closewith double-string neck pull.

Waste bins and bags are available ina variety of sizes.

Beta Storage Box, MaxiGamma Shield

Beta Adjust. Shield

Storage Box Insert, Mini

Beta StorageBox, Mini

Beta Storage Box, Mini

Storage Box Insert, Mini

Storage Box Insert, MiniWaste Bin, 10 L

Cabinet Workstation

Radiation Safety

Item NumberType ofBlocking Product Description

56Radiation Safety

Hoefer Radiation Safety Products Ordering Information

All products indicated “Beta” below are made from 10 mm thick opticalacrylic and block Beta-emitting isotopes such as 32P, 35S, 3H, and 14C.

BETA-BLOCKING PRODUCTS

All products indicated “Gamma” below are made from 12 mm thick lead-acrylic andblock Gamma-emitting isotopes such as 123I, 125I, 133Xe, 57Co, 99mTe, and 67Ga.

GAMMA-BLOCKING PRODUCTS

*Safety Precaution: 12 mm thick lead-acrylic is suitable only for protection from gamma emissions from 125I and not from higher isotopes of iodine.Danger: Do not use lead or lead-acrylic for shielding beta emissions.

Item NumberType ofBlocking Product Description

Gamma rectangular shield, 540 x 350 mm, with adjustable angle 0° to 15°Gamma rectangular shield, small (450 x 300 mm), with curved base and fixed 15° angleGamma rectangular shield, large (530 x 350 mm), with curved base and fixed 15° angleGamma rectangular shield, small (450 x 300 mm), with curved base and fixed 45° angleGamma hourglass shield, 450 x 300 mm, with curved baseMidi Gamma storage box with hinged lid, 60 x 165 x 85 mm ID (H x W x D)Midi storage box insert for 32 x 1.5 ml tubesMidi storage box insert for 40 x 0.5 ml tubesMidi storage box insert for 16 x 1.5 ml and 16 x 0.5 ml tubesMidi storage box insert for 32 x 2 ml CryotubesGamma pipette tip disposal box. Uses RPP-BAG17 Waste Bags.Gamma hinged-lid waste bin, 10 L capacity. Uses RPP-BAG31 waste bags.Heavy duty polyethylene waste bags with double string neck pull, 290 x 210 x 210 mm,for RPP-B27, RPP–GB27, and RPP-B31 bins, pk/25Radiation hazard symbol adhesive labels, 50 x 50 mm, pk/25

Shield, adjustableShield, fixed 15° angleShield, fixed 15° angleShield, fixed 45° angleShield, hourglassStorage box, MidiStorage box insert, MidiStorage box insert, MidiStorage box insert, MidiStorage box insert, MidiTip disposal boxWaste bin, 10 LWaste bag

Radiation hazard labels

GammaGammaGammaGammaGammaGamma

GammaGamma

RPP-GS015RPP-GS15SCRPP-GS15LCRPP-GS45SCRPP-GSHCRPP-GB6RPP-BI-32RPP-BI-40RPP-BI-8RPP-BI-9RPP-GB15SCRPP-GB27RPP-BAG31

RPP-LAB50

Beta rectangular shield, 540 x 350 mm, with adjustable angle 0° to 15°Beta rectangular shield, small (450 x 300 mm), with curved base and fixed 15° angleBeta rectangular shield, large (530 x 350 mm), with curved base and fixed 15° angleBeta rectangular shield, small (450 x 300 mm), with curved base and fixed 45° angleBeta hourglass shield, 450 x 300 mm, with curved baseMini Beta storage box with hinged lid, 55 x 85 x 85 mm (H x W x D)Midi Beta storage box with hinged lid, 60 x 165 x 85 mm ID (H x W x D)Maxi Beta storage box with hinged lid, 140 x 280 x 165 mm ID, (H x W x D)Mini storage box insert for 16 x 1.5 ml tubesMidi storage box insert for 32 x 1.5 ml tubesMidi storage box insert for 40 x 0.5 ml tubesMidi storage box insert for 16 x 1.5 ml and 16 x 0.5 ml tubesMidi storage box insert for 32 x 2 ml CryotubesMaxi storage box insert for 15 x 15 ml centrifuge tubesMaxi storage box insert for 8 x 50 ml centrifuge tubesBeta flipper box for 4-Way FlipperTM racks, 140 x 175 x 150 mm IDBeta storage and transport box, 70 x 150 x 120 mm ID,specially machined to accept 24 x 1.5 ml Eppendorf tubesBeta pipette tip disposal box. Uses RPP-BAG17 waste bags.Beta cabinet workstation with hinged doors, 490 x 550 x 370 mm IDBeta waste bin, 1 L capacityBeta waste bin, 3.3 L capacity. Uses RPP-BAG17 waste bags.Beta waste bin, 10 L capacity. Uses RPP-BAG31 waste bags.Beta waste bin, 15 L capacity. Uses RPP-BAG31 waste bags.Beta waste bin, 20 L capacity. Uses RPP-BAG40 waste bags.Heavy duty polyethylene waste bags with double string neck pull,120 x 120 x 120 mm, for RPP-B17 bin, pk/25Heavy duty polyethylene waste bags with double string neck pull,290 x 210 x 210 mm, for RPP-B27, RPP–GB27,and RPP-B31 bins, pk/25Heavy duty polyethylene waste bags with double string neck pull,370 x 210 x 240 mm, for RPP-B40 bin, pk/25Radiation hazard symbol adhesive labels, 50 x 50 mm, pk/25

Shield, adjustableShield, fixed 15° angleShield, fixed 15° angleShield, fixed 45° angleShield, hourglassStorage box, MiniStorage box, MidiStorage box, MaxiStorage box insert, MiniStorage box insert, MidiStorage box insert, MidiStorage box insert, MidiStorage box insert, MidiStorage box insert, MaxiStorage box insert, MaxiFlipper boxStorage and transport box

Tip disposal boxCabinet workstationWaste bin, 1 LWaste Bin, 3.3 LWaste Bin, 10 LWaste Bin, 15 LWaste Bin, 20 LWaste Bag

Waste Bag

Waste Bag

Radiation hazard labels

BetaBetaBetaBetaBetaBetaBetaBeta

BetaBeta

BetaBetaBetaBetaBetaBetaBeta

RPP-S015RPP-S15SCRPP-S15LCRPP-S45SCRPP-SHCRPP-B5RPP-B6RPP-B14RPP-BI-16RPP-BI-32RPP-BI-40RPP-BI-8RPP-BI-9RPP-BI-1RPP-BI-2RPP-FBRPP-STB

RPP-B15SCRPP-CRPP-B12RPP-B17RPP-B27RPP-B31RPP-B40RPP-BAG17

RPP-BAG31

RPP-BAG40

RPP-LAB50

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800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

1.1


Biochrom™

30 SeriesAmino Acid Analyzer

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Convenience: “Load and Go” post-column photometric detection of amino acids with no additional derivatization procedures.

The Biochrom 30 Series Amino AcidAnalyzer is a sophisticated PC controlledliquid ion exchange chromatographysystem dedicated to providing rapid,specific amino acid analysis for clinical,pharmaceutical, proteomics, food andfeedstuff industries.

Flexibility: Integrated software enables automatic sample set-up, flexible samples analysis, networking and exporting of data.

Versatility: A wide variety of sample types can be analyzed using the available range of programs and columns.

Bio 30

Biochrom 30 Amino Acid AnalyzerBiochrom 30 Amino Acid Analyzer

The Biochrom 30 Amino Acid Analyzers comprise instrument, 84 position cooled autosampler, analytical column with top-upresin, prewash column, operating software, EZchrom Elite™ data handling software, chemical kit with calibration standard,Ninhydrin and buffer bottles, instruction manuals, tool kit and spare parts/consumables kit. The system also includes PentiumPC and Deskjet printer. The systems offer:

Serviceability: Ergonomic design for ease of maintenance.

Biochrom 30 PhysiologicalBiochrom 30 Physiological

Designed for metabolic disorderscreening and research, a full routineanalysis of physiological fluids can beperformed enabling the determination ofup to 53 amino acids.

Biochrom 31 ProteinBiochrom 31 Protein

Designed for quality control in drugsynthesis and process control andauthenticity testing, the system can rapidlydeal with complex samples and high saltconcentrates.

Biochrom 32 Oxidized ProteinsBiochrom 32 Oxidized Proteins

Designed for the analysis of complexhydrolysates such as food and feedstuffs,the system meets the requirements of thestandard methods from the AOAC and theEU Commission Directive 98/64/EC.

60Bio 30

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Ultra Oxidized Feedstuff Chemical Kit.Sufficient for up to 360 hoursof continuous operation.80-2117-79

Hoefer is the exclusive distributor for the Biochrom 30 series oHoefer is the exclusive distributor for the Biochrom 30 series of Amino Acid Analyzers in the United States.f Amino Acid Analyzers in the United States.For information on distributors and support in other countries pFor information on distributors and support in other countries please visit the Biochrom Website at: www.biochrom.co.uklease visit the Biochrom Website at: www.biochrom.co.uk

SYSTEMS

Biochrom 30 SeriesAmino Acid Analyzers

Physiological InstrumentItem# 80-2111-00

Biochrom 30 Physiological SystemIncludes:Pentium PCDeskjet printerEZchrom Elite handling softwareItem# 80-2111-00-SYS

Protein Hydrolysate InstrumentItem# 80-2111-01

Biochrom 31 Protein Hydrolysate SystemIncludes:Pentium PCDeskjet printerEZchrom Elite handling softwareItem# 80-2111-01-SYS

Oxidized Hydrolysate InstrumentItem# 80-2111-02

Biochrom 32 Oxidized Hydrolysate SystemIncludes:Pentium PCDeskjet printerEZchrom Elite handling softwareItem# 80-2111-02-SYS

Biochrom 30 Series Ordering Information

REAGENTS

Sodium hydroxide solution, 1 LItem# 80-2037-57

Calibration standard physiological fluidItem# 80-2037-62

Lithium citrate buffer A, 2 LItem# 80-2038-15

Lithium citrate buffer B, 2 LItem# 80-2038-16

Lithium citrate buffer C, 2 LItem# 80-2038-17

Lithium citrate buffer D, 2 LItem# 80-2038-18

Lithium citrate buffer D II, 2 LItem# 80-2097-18

Lithium citrate buffer CII, 4151-IIItem# 80-2099-83

Lithium citrate buffer pH 3.55, 2 LItem# 80-2037-69

Borate/citrate buffer pH 8.6, 2 LItem# 80-2037-88

Sodium citrate buffer pH 2.2, 2 LItem# 80-2037-67






Lithium citrate buffer pH 2.2, 2 LItem# 80-2038-10

Lithium hydroxide solution, 1 LItem# 80-2038-20

Ultra Clean solventItem# 80-2117-75

Ultra Ninhydrin Reagent Kit 8 L80-2117-76

Ultra Ninhydrin Reagent Kit 2 L80-2118-30

Ultra Physiological Fluid Chemical Kit.Sufficient for up to 500 hoursof continuous operation.80-2117-77

Ultra Protein Hydrolysate Chemical Kit.Sufficient for up to 320 hoursof continuous operation.80-2117-78

800.227.4750 (USA) FAX 1.415.920.44941.415.282.2307 (INT.)

Economical Filtration Plates

48, 96 and 384 well formats

Many designs for different sample sizes

Large selection of pore sizes, materials and shapes

Ideal for the rapid purification of proteins, nucleic acids,carbohydrates and other biomolecules.

ElectroPrep has a broad range of membranes that itsupports. Membranes with MWCO (molecular weight cutoff) of:100, 500, 1K, 2K, 5K, 10K, 25K, 50K and100K are available.

With a run-time of 3 to 10 minutes, ElectroPrep providesspeed and convenience, even at the very low currents (5

to10 mA) used with this system. The sample chambers aremade of PTFE which is a completely inert material especially

suited for high sample recovery.

Membranes of different MWCO are available from 100 to100,000 MWCO and can be used for selective elution, filtration,

dialysis, fractionation and concentration.

A fast DIALYZER can be joined with up to 7 multiple link chambers in differentcombinations and membranes.

2D ElectroPrep

Sample Fractionation Device

The New ElectroPrep System is an extremely versatile patentedsample prep technology based on (2D) electrophoresis/dialysis.

The system can, in three minutes, dialyze a samplesimultaneously into multiple MWCO fractions. The system isalso ideal for iso-electric focusing of protein samples and canbe used to fractionate samples before 2D electrophoresis.

Harvard Apparatus61

HA

RV

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TUS HARVARD APPARATUS

Complete Solutions for your Sample

Preparation Requirements:dialysis, electrodialysis, equilibriumdialysis; filtration; Micro SPE.

Equilibrium Dialysis

For Binding Studies

Single sample or patented 96-well format

5K and 10K MWCO

Re-usable PTFE or disposable chambers

Easy-to-use

SBS compatible for automation

Membranes from 0.45-25µm (others available by request)

HA

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62Harvard Apparatus

For application assistance or a free catalogcall Harvard Apparatus at 800 -272-2775 orregister at www.harvardapparatus.com oremail us at [email protected].

Breakthrough In Sample Preparation

Patented C-18 PrepTipTM for manual or automated sample prep

No Backpressure makes PrepTipTM easy-to-use

High precision and accuracy of sample binding

Ideal for de-salting, buffer exchange, sample enrichment

CoZap is used for rapid removal of Coomassie Bluestain from electrophoresis gels without the need tochange the destaining solution. CoZap is a uniquepad that has high absorbance for Coomassie Blue andis thus very effective in destaining gels. CoZap absorbsany free dye in the solution making gel destaining20% faster than conventional methods.

No need to change the destaining solution

Fast and Simple

No charcoal or dye residues

No subsequent destaining required

One pad can destain up to 10 gels

20% faster than conventional method

CoZap™

The Quick and Easy Destainer

High Performance Spincolumns

Single or 96 well formats

Unrivaled selection of column chemistries:G10, 25, 50,100; P2, 6; C18,8, 4; CN, NH2, PVOH and PHEAStrong & weak anion and cation, charcoal, cellulose and IMAC

High sample recovery rates

Economically priced

Ideal for de-salting, buffer exchange and sample enrichment

Spincolumns varied sample sizes: 10-25 µl, 25-75 µl, 75-150 µl

3 µl bed volume, 10 µl tip volume

Technical Appendix63

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DIX Technical Appendix


Electrophoresis performed in Tris-glycine-SDS buffer.*The larger proteins fail to move significantly into the gel.NOTE: Proteins smaller than approximately 14 kD fail toresolve away from the ion front in a Tris-glycine buffersystem. A Tris-Tricine method is routinely used instead(Schagger, H. and Von Jagow, G. (1987). AnalyticalBiochem. 166, 368)

Separation size range(kD)

Recommended acrylamideconcentrations for protein separation

1 kb of DNA = 37,000 g/molAverage molecular weight of one amino acid = 120 g/mol

Common Conversions

% Acrylamidein resolving gel

36 - 20524 - 20514 - 20514 - 66*14 - 45*

5%7.5%10%

12.5%15%

*ReferenceSmejkal, G. and Gallagher, S., BioTechniques 16,197 (1994).

Mr (x103)

Recommended acrylamide concentrationsfor semi-dry transfer of proteins from a0.75-mm thick gel*

% Acrylamide

< 20< 36

14 - 66 29 - 150

�20%�15%�10%�5%

* Indicates protein intended use: S, SDS PAGE; I, IEF; N, non-denaturing PAGE

Protein marker characteristicsNativeMr (x103)SourceProtein Use*

SubunitMr (x103)

pl at24°C

� - 2 macroglobulin� - lactalbuminactinalbuminamyloglucosidaseaprotinin� - galactosidase� - lactoglobulin Acarbonic anhydrasecarbonic anhydrasecatalaseconalbumincreatine phosphokinasecytochrome cferritinGAPDHglucose oxidaseglutamate dehydrogenaseinsulin A chaininsulin B chainlactate dehydrogenaselentil lectinlysozymemyoglobinmyosinovalbuminpepsinogenphosphorylase bthyroglobulintransferrintriose phosphateisomerasetrypsin inhibitortrypsinogen

bovine plasmabovine milkbovine musclebovine serum

SSSN, SISSIS, IINIIINIISSSNISS, ISSISNSS

S, II

17014.44366

6.51163530

232

8112.4440140160532.53.5140

22045

976697653

20.1

17014.44366

6.511618.4303560764012.4220, 18.536

532.53.5365014.417.522045

973357626.5

20.124.0

5.0, 5.15.4, 5.5, 5.63.50

5.305.856.555.406.0, 6.3, 6.67.0010.254.23, 4.38, 4.508.3, 8.54.15

8.15, 8.45, 8.65

6.85, 7.25

4.72.8

5.8, 6.2, 7.0,7.7, 8.04.559.3

bovine liverbovinebovinebeef heartlentilegg whiteequine heartrabbit muscleegg whiteporcine stomachrabbit musclehog thyroidhumanrabbit

soybeanbovine pancreas

Aspergillus niger

Escherichia colibovine pancreas

bovine milkbovine erythrocytehumanbovine liveregg whiterabbit muscleequine hearthorse spleenrabbit muscleAspergillus niger

Nucleic Acid Electrophoresis

*From “Molecular Cloning: A Laboratory Manual”(Sambrook, J., Fritsch, E.F. and Maniatis, T., thirdedition, 2001).

Amount ofagarose in gel

(% [w/v])

Recommended agaroseconcentrations for DNA separation*

Effective range ofseparation of linearDNA molecules (kb)

0.30.60.70.91.21.52.0

5 - 601 - 20

0.8 - 100.5 - 70.4 - 60.2 - 30.1 - 2 †N,N’-methylenebisacrylamide is included at 1/30th the

concentration of acrylamide.‡The numbers given are the approximate sizes (innucleotide pairs) of fragments of double-stranded DNAwith which the dye comigrates.*From “Molecular Cloning: A Laboratory Manual”(Sambrook, J., Fritsch, E.F. and Maniatis, T., thirdedition, 2001).

Recommended acrylamideconcentrations for DNA separation*

Acrylamide(% [w/v])†

Effective rangeof separation

(bp)

3.55.08.012.015.020.0

1000 - 200080 - 50060 - 40040 - 20025 - 1506 - 100

XylenecyanolFF‡

Bromophenolblue‡

460260160706045

1006545201512

†The numbers are the approximate sizes ofDNA (in nucleotides) with which themarker dye comigrates.*From “Molecular Cloning: A LaboratoryManual” (Sambrook, J., Fritsch, E.F. andManiatis, T., third edition, 2001).

Migration rates of marker dyesthrough denaturingpolyacrylamide gels*

%Polyacrylamide

5681020

Xylene cyanolFF†

Bromophenolblue†

352619128

130106765528

Electrophoresis

DUAL COOLED VERTICAL ELECTROPHORESIS UNITS

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64Online Support

hoeferinc.comOnline Support for Electrophoresis

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ULTimaIMAGING PLATFORMS

Contact Information65

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Contact Information

Email:Email:If you are interested in owning our products or needtechnical support, call us or email us at:

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On the Web:On the Web:Our website provides product and contact information forcustomers and contact information for vendors and distributors.Easy to complete forms help you register so you can receiveproduct information and special offers. Hoefer is available online at:

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66Terms & Conditions

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Bibliography67

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Brozinick Jr, JT, et. al., 1-[N,O-Bis-(5-isoquinolinesulphonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN-62), an Inhibitor of Calcium-DependentCamodulin Protein Kinase II, Inhibits Both Insulin- and Hypoxia-Stimulated Glucose Transport in Skeletal Muscle. BiochemicalJournal 339:533-540, 1999.

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Couce, ME, et. al., Analysis of K-ras, N-ras, H-ras, and p53 in LungNeuroendocrine Neoplasms. Diagnostic Molecular Pathology8(2):71-79, Jun 1999.

de la Cruz, M, et. al., DNA Isolation and Amplification from Cacti.Plant Molecular Biology Reporter 15:319-325, 1997.

Diehl, S, et. al., Analysis of Stage-Specific Gene Expression in theBloodstream and the Procyclic Form of Trypanosoma brucei Using aGenomic DNA Microarray. Molecular & Biochemical Parasitology123:115-/123, 2002.

Driscoll, MD, et. al., Sequence Requirements for Estrogen ReceptorBinding to Estrogen Response Elements. Journal of BiologicalChemistry 273(45): 29321-29330, 6 Nov 1998.

Engelbrecht, CJB, et. al., Genetic Variation in Eastern North Americanand Putatively Introduced Populations of Ceratocystis fimbriata f.platani. Molecular Ecology 13:2995-3005, 2004.

Ewings EL, et. al., Genotoxic and Functional Consequences ofTransplacental Zidovudine Exposure in Fetal Monkey Brain Mitochondria.Journal of Acquired Immune Deficiency Syndromes 24(2):100-105,1 June 2000.

Gao, Z, et. al., Palmitoylation of the Recombinant Human A1 AdenosineReceptor: Enhanced Proteolysis of Palmitoylation-Deficient MutantReceptors. Biochemical Journal 342:387-395, 1999.

Gerber, I, et. al., Stimulatory Effects of Creatine on Metabolic Activity,Differentiation and Mineralization of Primary Osteoblast-Like Cells inMonolayer and Micromass Cell Cultures. European Cells andMaterials 10:8-22, 2005.

Gerschenson, M, et. al., Fetal Mitochondrial Heart and Skeletal MuscleDamage in Erythrocebus patas Monkeys Exposed in Utero to 3'-Azido-3'-Deoxythymidine. AIDS Research and Human Retroviruses16(7):635-44, 01 May 2000.

Goodwin, SB, et. al., DNA Fingerprint Probe from Mycosphaerellagraminicola Identifies an Active Transposable Element.Phytopathology 91(12):1181-1188, 2001.

Gorton, TS, et. al., Physical Mapping of the Mycoplasmagallisepticum S6 Genome with Localization of Selected Genes.Journal of Bacteriology 177(1):259-263, Jan 1995.

Gruffat, D, et. al., Developmental Changes in Apolipoprotein B GeneExpression in the Liver of Fetal Calves. Biology of the Neonate 74:233-242, 1998.

Hanson, DC, et. al., UK-78,282, a Novel Piperidine Compound thatPotently Blocks the Kv1.3 Voltage-Gated Potassium Channel and InhibitsHuman T Cell Activation. British Journal of Pharmacology126(8):1707-16, Apr 1999.

Hanson, SF, and Maxwell, DP, trans-Dominant Inhibition of GeminiviralDNA Replication by Bean Golden Mosaic Geminivirus rep GeneMutants. Phytopathology 89(6):480-486, 1999.

Hu, J, and Bock, D, A Cell Culture Medium that Supports theDifferentiation of Human Retinal Pigment Epithelium into FunctionallyPolarized Monolayers. Molecular Vision 7:14-19, 7 February 2001.

Hu, L, et. al., Two-Dimensional Protein Database of Human Pancreas.Electrophoresis 25:512–518, 2004.

Krieg A, et. al., Expression of Different Survivin Variants in GastricCarcinomas: First Clues to a Role of Survivin-2B in Tumour Progression.British Journal of Cancer 86(5):737-43, 4 Mar 2002.

Lai, I-R, et. al., The Protective Role of Heme Oxygenase-1 on the Liverafter Hypoxic Preconditioning in Rats. Transplantation 77(7):1004-1008, 15 April 15 2004.

Lee, SG, and Calhoun, DH, Urease from a Potentially PathogenicCoccoid Isolate: Purification, Characterization, and Comparison to OtherMicrobial Ureases. Infection and Immunity 65(10):3991-3996, Oct1997.

Lin, C-J, and Smith, DE, Glycylsarcosine Uptake in Rabbit Renal BrushBorder Membrane Vesicles Isolated from Outer Cortex or Outer Medulla:Evidence for Heterogeneous Distribution of Oligopeptide Transporters.AAPS PharmSci 1(2):article 1, 1999.

Lindberg, T, et. al., In Vitro Digestion of Proteins in Human MilkFortifiers and in Preterm Formula. Journal of PediatricGastroenterology & Nutrition. 27(1):30-36, July 1998.

Liou, Y-M, et. al., Altered Expression of Cardiac Myosin IsozymesAssociated with the Malignant Hyperthermia Genotype in Swine.Anesthesiology 93(5):1312-1319, Nov 2000.

Lu, K, et. al., Antimicrobial Resistance Gene Delivery in Animal Feeds.Emerging Infectious Diseases Journal [serial online] 10(4), Apr2004 (date cited). Available fromhttp://www.cdc.gov/ncidod/EID/vol10no4/03-0506.htm.

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Mahmood, K, et. al., Human Cytomegalovirus Plasmid-Based AmpliconVector System for Gene Therapy. Genetic Vaccines and Therapy 3:1,2005.

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Matyasek, R, et. al., NTRS, a New Family of Highly Repetitive DNAsSpecific for the T1 Chromosome of Tobacco. Chromosoma 106:369-379, 1997.

Melody, JL, et. al., Early Postmortem Biochemical Factors InfluenceTenderness and Water-Holding Capacity of Three Porcine Muscles.Journal of Animal Science 82:1195-1205, 2004.

Menell, JS, et. al., Annexin II and Bleeding in Acute PromyelocyticLeukemia. New England Journal of Medicine 340(13):994-1004,1 Apr 1999.

Moneo, I, et. al., Immunodetection of Specific IgE (Immunoblotting) in aPrevalence Study of Anisakis simplex Sensitisation in Spain.Alergologia e Inmunologia Clinica 15:255-61, 2000.

Narayanan, S, et. al., Comparative Evaluation of PCR using IS6110 anda New Target in the Detection of Tuberculous Lymphadenitis. CurrentScience 78(11):1367-1370, 10 Jun 2000.

Noriega, R, et. al., Ecdysteroids and Oocyte Development in the BlackFly Simulium vittatum. BMC Developmental Biology [serial online]2:6, 24 Apr 2002. Available from http://www.biomedcentral.com/1471-213X/2/6.

Park, O, et. al., Calcium Binding of Phosphopeptides Derived fromHydrolysis of �-Casein or �-Casein Using Immobilized Trypsin. Journalof Dairy Science 81(11):2850-2857, 1998.

Patocs, A, et. al., Clinical and Biochemical Features of Sporadic andHereditary Phaeochromocytomas: an Analysis of 41 Cases Investigated ina Single Endocrine Centre. European Journal of Cancer Prevention13(5):403-409, October 2004.

Rollo, EE, et. al., The Cytokine Osteopontin Modulates the Severity ofRotavirus Diarrhea. Journal of Virology 79(6): 3509–3516, Mar2005.

Sarmiento, M, High Resolution, Standard Format Two-DimensionalProtein Electrophoresis using Disposable Glass Micropipettes and Non-Dedicated Equipment. Electrophoresis 16(1):431-437, 1995.

Schubeler, D, et. al., Genomic Targeting of Methylated DNA: Influence ofMethylation on Transcription, Replication, Chromatin Structure, andHistone Acetylation. Molecular and Cellular Biology 20(24):9103-9112, Dec 2000.

Skorska, C, et. al., Effects of Repeated Inhalation Exposure toMicrovesicle-Bound Endotoxin. Annals of Agricultural andEnvironmental Medicine 3:61-65, 1996.

Theopold, U, et. al., CalpA, a Drosophila Calpain Homolog SpecificallyExpressed in a Small Set of Nerve, Midgut, and Blood Cells. Molecularand Cellular Biology 15(2): 824–834, Feb 1995.

Tio, PH, et. al., Two Dimensional VOPBA Reveals Laminin Receptor(LAMR1) Interaction with Dengue Virus Serotypes 1, 2 and 3. VirologyJournal 2:25, 25 March 2005.

Ueno, M, et. al., A High-Fructose Diet Induces Changes in pp185Phosphorylation in Muscle and Liver of Rats. Brazilian Journal ofMedical and Biological Research 33(12):1421-1427, Dec 2000.

Volk, GM, and Franceschi, VR, Localization of a Calcium Channel-LikeProtein in the Sieve Element Plasma Membrane. Australian Journal ofPlant Physiology 27(9):779-784, 2000.

Yamamoto, K, et. al., Experimental Infection of Domestic Cats withBartonella koehlerae and Comparison of Protein and DNA Profiles withThose of Other Bartonella Species Infecting Felines. Journal ofClinical Microbiology 40(2):466-474, Feb 2002.

Zhou, J, et. al., RNA Isolation without Gel Formation fromOligosaccharide-Rich Onion Epidermis. Plant Molecular BiologyReporter 17: 397–407, 1999.

Please note that we have a large publications database that cannotbe accommodated in this catalog. For references on specificapplications please contact Hoefer at [email protected].

For theory and practice of a wide rangeof electrophoresis techniques Hoeferrecommends Dr. Reiner Westermeier's“Electrophoresis in Practice” publishedby Wiley-VCH (ISBN 3-527-30354-5).

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CTS

Call on our experts. We’ll analyze yourCall on our experts. We’ll analyze yourrequirements, determine the most efficient wayrequirements, determine the most efficient wayto meet them, and offer a price you will like.to meet them, and offer a price you will like.

Hoefer has been pioneering design and manufacture of electrophorHoefer has been pioneering design and manufacture of electrophoresisesisapparatus and related equipment for biotechnology research for oapparatus and related equipment for biotechnology research for over threever threedecades. We have the skilled craftsmen and state of the ardecades. We have the skilled craftsmen and state of the art equipment tot equipment tomanmanufufacture the practure the products descroducts described in this cataloibed in this catalogg..

If you need parts or products similiar to those found in this catalog -or have a new design idea that needs to be materialized; pleasecontact us and we will be happy to produce it for you.

In addition to our CNC machiningcapabilities, Hoefer’s custommachine shop boasts:

Conventional milling withBridgeport J series mills

Conventional turning withClausing Colchester, 15"swing lathes

Manually operated sawing, drilling,and tapping machines

Finish options including beadblasting and buffing

800.227.4750 (USA)

FAX 1.415.920.44941.415.282.2307 (INT.)

Programming with Mastercam 9.1and Geopath 4.1, andcompatibility with mostpopular CAD software

Plastic fabricating in our well-equipped solvent bonding room

Custom Products

For inquiries please contact your local Hoefer representative below:

Caliber, Chroma, Deca-Probe, Easy Breeze, Hoefer, ImmunoBlot,Macrovue, Max, Mighty Small, miniVE, Sturdier, PlateMate,WonderWedge, ULTima are trademarks of Hoefer, Inc.

Cy, CyDye and ECL are trademarks of GE Healthcare.Coomassie is a trademark of ICI, Plc.Novex is a trademark of Invitrogen Corp.CoZap and PrepTip are trademarks of Harvard Bioscience.Biochrom is a trademark of Biochrom Limited.TotalLab is a trademark of Nonlinear Dynamics.SYPRO and SBYR are trademarks of Molecular Probes.Tween is a trademark of ICI Americas, Inc.Nalgene is a trademark of Nalge Nunc International.Teflon and Mylar are trademarks of E.I. duPont de Nemours and Company.Tygon is a trademark of Norton Company.Microsoft Windows is a trademark of Microsoft, Inc.EZChrom Elite is a trademark of Agilent Technologies.

©2005 Hoefer, Inc. - All rights reserved.

Printed in the USA.

Hoefer, Inc.953 Indiana StreetSan Francisco, CA 94107 USAHoefer is a Harvard Bioscience company.

www.hoeferinc.com

Toll Free: 1-800-227-4750Local Phone: 1-415-282-2307Customer Service: Select 1 from the main menuTechnical Support: Select 2 from the main menu.Fax: 1-415-920-4494 or 1-415-821-0239Online Ordering: www.hoeferinc.comEmail: [email protected]

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