high performance liquid chromatography
TRANSCRIPT
HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
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CONTENTS:
The components of the high performance liquid chromatograph (HPLC).
The separation process.
The chromatogram.
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Component:Solvent
Pumps
Column
Detector
Chromatogram
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Separation in based upon differential migration between the stationary and mobile phases.
Stationary Phase - the phase which remains fixed in the column, e.g. C18, Silica
Mobile Phase - carries the sample through the stationary phase as it moves through the column.
Injector
Detector
Column
Solvents
Mixer
Pumps
High Performance Liquid Chromatograph
Waste
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Injector
Detector
Column
Solvents
Mixer
Pumps
Chromatogram
Start Injection
mAU
time
High Performance Liquid Chromatograph
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Injector
Detector
Column
Solvents
Mixer
Pumps
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time
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Injector
Detector
Column
Solvents
Pumps
Mixer
Chromatogram
Start Injection
mAU
time