hexamethyldisiloxane-based dual- modality dual-functional ... · of mcf7-g od (figure 1 lity...

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1 Jo Int las all PF he [3] for Me de res (po us vs 37 an us co Fig in sh up Re co (IC ox inc vis ov Co ba co flu Re NM Ac CA (c) He oint program i Dal troduction: st two decad been used FC nanoem examethyldis ]. In this stu r cellular an ethods: HM e-ionized wa spectively w olycarbonat sing DLS, TE pO 2 calibra 7 o C. In vitro nd time-depe sing confoca ontaining sep gure 1: (a) different lay howing oxyg ptake of the esults: The onsistent pa C 50 ). Dual m xygen consu creasing pO sualized in t ver the obse onclusion: ased imagin onsumption uorescence eferences: MR in Biome cknowledge A126608A. (a) Water MR Image examethyld in biomedical e llas, Texas, Un : There has des. Gadoli as imaging mulsions we siloxane (HM udy, HMDSO d molecular MDSO-base ater and sur were homo te membran EM and. She ation curve o o cytotoxicity endent upta al microscop parate layer White light a yers of matr gen consump nanoprobes e NEs were rticle size in modality ima umption by M O 2 gradient the cytosol ervation peri Dual-moda g and oxime in MCF-7 ce detection si 1) Bulte JW edicine, epu ement: Gra (b HM MR disiloxane-b Jyothi Meno engineering, U nited States, 3 S s been a gro nium chelat g agents to ere used in MDSO) rep O based du r imaging. ed nanoemu rfactant mix ogenized us ne filter diam elf life and p of the NE wa y of NEs an ake of Nile py. Additiona rs of basem and (b) fluo rigel (c) Tim ption. (e) M s by MCF7- e measured n DI water aging was s MCF7 cells across the of MCF7-G od (Figure 1 lity dual-fun etry and fluo ells. These multaneous W et al., NMR ub ahead of ant support f b) DSO Image based dual- on 1,2 , Praveen G UT Arlington/U School of Biolo owing intere tes, superpa non-invasiv n 19 F MR orter molec al modality ulsions (NEs xture (Polye sing an ult meter - 50 n particle size as determin d surfactant red-doped N ally, the dua ment membra rescence im me-course pO Maximum inte -GFP cells o d to have a and 50% hu uccessfully (Figure 1 (c e labeled m FP breast c 1 (e)). nctional HMD orescence i results indic sly. R Biomed 17 f print DOI:1 from Norma (d) -modality d Gulaka 1 , Mada UT Southweste ogical and Hea est in develo aramagnetic vely monitor oximetry [2 ules can be (MRI/Fluore s) were pre ethylene glyc trasonic ho nm), to obta variations w ed using a s t combinatio NEs by MC al-functional ane matrige mages of a s O 2 maps sho ensity proje over a perio n average uman plasm performed c), (d)), using atrigel. Add cancer cells DSO-based maging. As cate that the 7(7):p 484-4 10.1002/nbm an Hackerma pO 2 overla dual-functio alyn McKay 2 , S ern Medical Ce alth Systems En oping MR co c iron oxide cellular pro 2] while it e used to qu escence), d pared by a col hydroxy omogenizer. ain uniform p were assess spin-echo ba on on 3T3 fi CF-7 cells tra ity of the NE el containing sample cont owing oxyge ection of con d of 30 mins % mass w ma for 24 h on matrigel g these nan ditionally, N and uptake d nanoprobe proof of pri e HMDSO-b 499 (2004). 2 m.1678. 4) K an ARP gra ay onal nanop Sairam Geetha enter, Dallas, T ngineering, Ar ontrast agen (SPIO) par ocesses or t was recen antitatively dual-function single emu ystearate (H The samp particle size sed in DI wat ased pulse ibroblasts w ansfected w Es was teste g MCF7 and taining Nile- en consump nfocal micro s. weighted rad ours and sh l phantoms noprobes. A ile red fluo e was obser es were succ nciple, thes based nanoe 2) McIntyre Kodibagkar ant #01001 probes for c anath 1 , and Vik Texas, United rizona State Un nts for cellu rticles and p the behavio tly demons measure ox nal (oximetr ulsion metho HS-15) and s ple was th e distribution ter and hum sequence b were studied with GFP (G ed by fluore d Nile red-do -red doped d ption in the s scopy comp dius of abou howed cyto to show flu A pO 2 drop o rescent dye rvable withi cessfully sy se were suc emulsions c et al. Curr. S VD et al. NM 9-0056-200 (e) cellular and kram D Kodiba States, 2 Radio niversity, Tem ular and mol perfluorocar r of macrom strated that xygen tensio ry/detection) od in which soybean lec en extrude n. Characte man plasma. by spectrosc d using MTS Green Fluore scence and oped NEs. dual functio same samp posite image ut 70.62 nm compatibilit uorescence of 60 torr wa e-doped na n 18 minute ynthesized a cessfully us can be effec Sci. 1999; 7 MR Biomed 07, NCI 1R2 d molecular agkar 1,3 ology, UT Sout pe, Arizona, U lecular imag rbon (PFC) molecules in t nanoemul on (pO 2 ) in t ) nanoemul h a solution cithin) in rat ed through erization of t Longitudina copy in a Va S assay. Intr escent Prot d PISTOL[4] onal nanopro ple (d) Mean es showing m. The nan ty up to 0.4% (Figure 1 (a as observed anoemulsion es of incuba and characte sed for study ctively used 76: 753–762 d. 2008; 21(8 21CA132096 r imaging thwestern medi United States ging especia nanoemulsi n vivo [1]. P sions enca tissues usin sions were containing tios of 40:55 a Lipofast these NEs w al relaxation arian 4.7 T s racellular lo tein) was de imaging of obes and M n pO 2 timeco time-depen noprobes m %(v/v) conc a), (b)) and over 4 hour ns were suc ation and pr erized for 1 H ying oxygen for oximetry 2. 3) Gulaka 8): 899–907 6-01A1 and ical center, ally in the ions have reviously, apsulating ng 1 H MRI prepared HMDSO, 5:5 % v/v extruder was done n rate (R 1 ) scanner at calization etermined a sample CF7 cells ourse ndent maintained centration measure rs with an ccessfully rogressed H MRI n y and PK et al., 7. d NCI U24 4335 Proc. Intl. Soc. Mag. Reson. Med. 20 (2012)

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Page 1: Hexamethyldisiloxane-based dual- modality dual-functional ... · of MCF7-G od (Figure 1 lity dual-fun try and fluo lls. These multaneous et al., NM R b ahead of nt support f) DSO

1Jo

IntlasallPFhe[3]for Mederes(pousvs37anusco

Figin shup

Reco(ICoxincvisovCobacoflu

ReNM

AcCA

(c)

He

Joint program iDal

troduction:st two decad been used

FC nanoemexamethyldis]. In this stur cellular an

ethods: HMe-ionized waspectively wolycarbonat

sing DLS, TE pO2 calibra

7 oC. In vitrond time-depesing confocaontaining sep

gure 1: (a) different lay

howing oxygptake of the

esults: Theonsistent paC50). Dual mxygen consucreasing pOsualized in tver the obseonclusion: ased imaginonsumption uorescence

eferences: MR in Biome

cknowledgeA126608A.

(a)

Water MR Image

examethyld

in biomedical ellas, Texas, Un

: There hasdes. Gadoli as imaging

mulsions wesiloxane (HM

udy, HMDSOd molecular

MDSO-baseater and surwere homote membranEM and. Sheation curve oo cytotoxicityendent upta

al microscopparate layer

White light ayers of matrgen consumpnanoprobes

e NEs wererticle size in

modality imaumption by MO2 gradient the cytosol

ervation periDual-modag and oximein MCF-7 cedetection si

1) Bulte JWedicine, epu

ement: Gra

(b

HMMR

disiloxane-bJyothi Meno

engineering, United States, 3S

s been a gronium chelat

g agents to ere used inMDSO) repO based dur imaging.

ed nanoemurfactant mixogenized usne filter diamelf life and pof the NE way of NEs anake of Nile py. Additionars of basem

and (b) fluorigel (c) Timption. (e) Ms by MCF7-

e measuredn DI water

aging was sMCF7 cells across the

of MCF7-God (Figure 1lity dual-funetry and fluoells. These multaneous

W et al., NMRub ahead of

ant support f

b)

DSO Image

based dual-on1,2, Praveen GUT Arlington/USchool of Biolo

owing interetes, superpanon-invasivn 19F MR orter molecal modality

ulsions (NEsxture (Polyesing an ultmeter - 50 nparticle size as determind surfactantred-doped Nally, the dua

ment membra

rescence imme-course pOMaximum inte-GFP cells o

d to have aand 50% huuccessfully (Figure 1 (c

e labeled mFP breast c1 (e)).

nctional HMDorescence iresults indicsly.

R Biomed 17f print DOI:1

from Norma

(d)

-modality dGulaka1, MadaUT Southwesteogical and Hea

est in develoaramagnetic

vely monitor oximetry [2ules can be(MRI/Fluore

s) were preethylene glyctrasonic honm), to obtavariations wed using a st combinatioNEs by MCal-functionalane matrige

mages of a sO2 maps shoensity projeover a perio

n average uman plasmperformed

c), (d)), usingatrigel. Add

cancer cells

DSO-basedmaging. As cate that the

7(7):p 484-410.1002/nbm

an Hackerma

pO2 overla

dual-functioalyn McKay2, Sern Medical Cealth Systems En

oping MR coc iron oxidecellular pro

2] while it e used to quescence), d

pared by a col hydroxy

omogenizer.ain uniform pwere assessspin-echo baon on 3T3 fi

CF-7 cells traity of the NE

el containing

sample contowing oxyge

ection of cond of 30 mins

% mass wma for 24 hon matrigelg these nanditionally, N and uptake

d nanoprobeproof of pri

e HMDSO-b

499 (2004). 2m.1678. 4) K

an ARP gra

ay

onal nanopSairam Geethaenter, Dallas, Tngineering, Ar

ontrast agen (SPIO) par

ocesses or twas recenantitatively

dual-function

single emuystearate (H The sampparticle size

sed in DI watased pulse ibroblasts wansfected wEs was testeg MCF7 and

taining Nile-en consumpnfocal micros.

weighted radours and shl phantoms

noprobes. A ile red fluo

e was obser

es were succnciple, thes

based nanoe

2) McIntyre Kodibagkar

ant #01001

probes for canath1, and VikTexas, United rizona State Un

nts for cellurticles and pthe behaviotly demonsmeasure oxnal (oximetr

ulsion methoHS-15) and sple was the distributionter and humsequence b

were studiedwith GFP (Ged by fluored Nile red-do

-red doped dption in the sscopy comp

dius of abouhowed cytoto show flu

A pO2 drop orescent dyervable withi

cessfully syse were sucemulsions c

et al. Curr. SVD et al. NM

9-0056-200

(e)

cellular andkram D KodibaStates, 2Radio

University, Temp

ular and molperfluorocarr of macrom

strated thatxygen tensiory/detection)

od in whichsoybean lecen extruden. Characte

man plasma.by spectroscd using MTSGreen Fluorescence andoped NEs.

dual functiosame sampposite image

ut 70.62 nmcompatibilit

uorescence of 60 torr wae-doped nan 18 minute

ynthesized acessfully us

can be effec

Sci. 1999; 7MR Biomed

07, NCI 1R2

d molecularagkar1,3

ology, UT Soutpe, Arizona, U

lecular imagrbon (PFC) molecules int nanoemulon (pO2) in t) nanoemul

h a solution cithin) in rat

ed through erization of t Longitudina

copy in a VaS assay. Intrescent Prot

d PISTOL[4]

onal nanoprople (d) Meanes showing

m. The nanty up to 0.4%(Figure 1 (a

as observed anoemulsiones of incuba

and charactesed for studyctively used

76: 753–762d. 2008; 21(8

21CA132096

r imaging

thwestern mediUnited States

ging especiananoemulsi

n vivo [1]. Psions encatissues usinsions were

containing tios of 40:55a Lipofast

these NEs wal relaxation

arian 4.7 T sracellular lotein) was de imaging of

obes and Mn pO2 timecotime-depen

noprobes m%(v/v) conca), (b)) and over 4 hour

ns were sucation and pr

erized for 1Hying oxygenfor oximetry

2. 3) Gulaka 8): 899–907

6-01A1 and

ical center,

ally in the ions have reviously,

apsulating ng 1H MRI

prepared

HMDSO, 5:5 % v/v

extruder was done n rate (R1) scanner at calization

etermined a sample

MCF7 cells ourse ndent

maintained centration measure

rs with an ccessfully rogressed

H MRI n y and

PK et al., 7.

d NCI U24

4335Proc. Intl. Soc. Mag. Reson. Med. 20 (2012)