hematopoietic and lymphatic system. blood parasites
TRANSCRIPT
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Hematopoietic and Lymphatic System
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Blood Parasites
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Trypanosoma
• Trypanosoma cruzi
Causing chagase disease.
• Trypanosoma bruci
African sleeping disease.
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morphological forms of Hemoflagellate
•
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Trypanosoma gambianse brucii
• Vector Tse Tse fly.
• Trypomastigote stage in the blood stream.
• African sleeping disease.
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Life Cycle of Trypanosoma brucei spp
Life
Cycle
of
Trypanosoma
brucei
spp
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Trypanosoma cruzi
Vector: reduviid bugs.
Disease: American Chagas disease.
Trepomastigote stage in blood.
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"pseudocysts" of Trypanosoma cruzi
• containing the amastigote stage in
heart muscle
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Life Cycle:
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Plasmodium spp.
• P. falciparum.
• P. ovale.
• P. vivax.
• P.malariae
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Plasmodium Falciparum
a. Multiple ring stage inside RBC.
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Plasmodium Falciparum
• Cresent or banana shape gametocyte
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Plasmodium spp.
• Gametocytes of • P. ovale.P. vivax.• P.malariae.• Round in shape• Male -->Micro
gamete --> diffuse.• Female --> macro
gamete --> condensed.
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Plasmodium life cycle
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Leishmaniasis
• Leishmaniasis is transmitted through the bite of female phlebotomine sandflies.
• Leishmania donovani (Visceral VL)
• Leishmania tropica (Cutaneous CL)
• Leishmania braziliensis ( Mucocutaneous leishmaniasis MCL)
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Leishmania spp.
• Promastigotes stage in sand flies.
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• Amastigote in the tissue.
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Leishmania life cycle•
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Bacteriology
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Media and additives present in blood culture bottle
• Several media are used for blood culture bottles like.
• Trypticase soy broth. Thioglycolate media, Brain heart infusion.additives SPS (sodium poly anethel sulfonate)
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Media used for Isolation
• Blood Media: most commonly used.
• Chocolate agar :
• Thioglycolate media: (enriched)
• MacConkey agar: Enterobacteria.
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inoculation
• . And we do the culture by streaking method.
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Swap method
take the swap under sterile condition, rotate it on the first quadrant of blood agar plate and replace it in a thioglycolate broth.
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Streptococcus pyogenes
• Colonies of Streptococcus pyogenes on sheep blood agar.
• Notice: * Presence of b hemolysis around colonies
• * Enhanced hemolysis around stabbing sites
• * Sensitivity to bacitracin (Disk A)
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Streptococcus pyogenes
• Pin point colony: (white or gray)
• Gram stain: G+ve, cocci, single chain.
• Catalase enzyme: differentiate between
Streptococcus –ve
Staphylococcus +ve
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Staphylococcus aureus
• G-positive cocci in clusters,
• typical of Staphylococcus aureus
• Catalase test used to differentiate Staphylococci
• from Streptococci
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• Coagulase test used to
differentiate S. aureus
• from other Staphylococcus spp.
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• Mannitol salt agar: selective and differential for Staphylococcus spp.
• (Staphylococcus aureus manitol fermenteryellow)
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Pseudomonas aeruginosa
• Greenish discoloration of media due to production of pyocyanin by Pseudomonas aeruginosa
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Pseudomonas aeruginosa
• Results of oxidase test
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E. coli
• G-negative bacilli,• typical of E. coli
• Colonies of E. coli on MacConkey
agar
• (Pink color indicates lactose
fermentation)
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• IMViC reaction of E. coli:
• + + - -
KIA A/A
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Proteus spp.
• Proteus - Members of the genus Proteus will swarm at certain intervals and produce a pattern of rings due to their motility.
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Urease Test, H2S
• (Positive Urease Test)
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