healthy control
DESCRIPTION
Set of gel images for analysis. Healthy Control. Case. 2D gel images of Serum protein sample. Steps followed in gel analysis. 1) Set up Project 2) Detect and Quantify Spots 3) Analyze Expression Profiles 4) Present Results. ANALYSIS, RESULT AND DATA INTERPRETATION. - PowerPoint PPT PresentationTRANSCRIPT
Healthy Control Case
2D gel images of Serum protein sample
Set of gel images for analysis
Steps followed in gel analysis
1) Set up Project
2) Detect and Quantify Spots
3) Analyze Expression Profiles
4) Present Results
ANALYSIS, RESULT AND
DATA INTERPRETATION
Project Name Serum sample 2D-Gels for Analysis.Project Creation Date ……………….Author ……………….Description Healthy vs diseased patientUse internal Standard NoPool C\Users\XYZ
GroupsControl(1 image)Control - Healthy person349spots transferred from fused image97 spots cancelledDiseased (1 image)P.falciparum diseased serum sample349 spots transferred from fused image97 spots cancelledVector Matching : 109
1. Project Summary:
Profile Spot Information Control Diseased
Spot ID : 1999 pI : 5.26 MW : 65.1kDal
3D view
Spot ID : 2082 pI : 5.28 MW : 44.8kDal
3D view
Control P.fal Fused
Control P.fal Fused
3D view
3D view
1. Project Summary:
4.00 4.25 4.50 4.75 5.00 5.25 5.50 5.75 6.00 6.25 6.50 6.75 7.00
80
75
70
65
60
55
50
45
40
35
30
25
20
15
10
5
pI index
Mol
ecul
ar w
eigh
t (kD
al)
85
Spot ID: 2082 pI : 5.28 Mol. weight : 44.8 kDal
Spot ID: 1999 pI : 5.26 Mol. weight : 65.2 kDal
3.Figure showing Molecular weight to pI
No.
of s
pots
sho
win
g up
regu
latio
n
Up regulation No. of folds(and above) No.of spots
2 693 474 395 338 21
10 1615 1520 1025 850 375 1
Up regulationx Folds or above
4.1. Differential expression : Up regulation
A B C D E F
G H I J K
Mean normalized value of group-Control(Healthy person)
Mean normalized value of group 2 (P.falciparum diseased person) Ratio =
4.2. expression profile: Up regulation(8-10 folds up) -11 spots
Down RegulationNo. of folds(and above) No. of spots
2 83
3 48
4 37
5 28
7 13
10 11
15 5
30 2
No.
of s
pots
sho
win
g up
regu
latio
n
Down regulationx Folds or above
4.3.Differential expression : Down regulation
4.4.Expression profile: Down regulation (9-10 folds down) - 9 spots
A B C D E
F G H I
Mean normalized value of group-Control(Healthy person) Ratio =
Mean normalized value of group 2 (P.falciparum diseased person)
Hierarchical Cluster Analysis HCL Tree
It provides information about similarly expressed protein.
It helps in finding correlated protein
5.Cluster Analysis
6. Scatter plot