Healthy Control Case

2D gel images of Serum protein sample

Set of gel images for analysis

Steps followed in gel analysis

1) Set up Project

2) Detect and Quantify Spots

3) Analyze Expression Profiles

4) Present Results

ANALYSIS, RESULT AND

DATA INTERPRETATION

Project Name Serum sample 2D-Gels for Analysis.Project Creation Date ……………….Author ……………….Description Healthy vs diseased patientUse internal Standard NoPool C\Users\XYZ

GroupsControl(1 image)Control - Healthy person349spots transferred from fused image97 spots cancelledDiseased (1 image)P.falciparum diseased serum sample349 spots transferred from fused image97 spots cancelledVector Matching : 109

1. Project Summary:

Profile Spot Information Control Diseased

Spot ID : 1999 pI : 5.26 MW : 65.1kDal

3D view

Spot ID : 2082 pI : 5.28 MW : 44.8kDal

3D view

Control P.fal Fused

Control P.fal Fused

3D view

3D view

1. Project Summary:

4.00 4.25 4.50 4.75 5.00 5.25 5.50 5.75 6.00 6.25 6.50 6.75 7.00

80

75

70

65

60

55

50

45

40

35

30

25

20

15

10

5

pI index

Mol

ecul

ar w

eigh

t (kD

al)

85

Spot ID: 2082 pI : 5.28 Mol. weight : 44.8 kDal

Spot ID: 1999 pI : 5.26 Mol. weight : 65.2 kDal

3.Figure showing Molecular weight to pI

No.

of s

pots

sho

win

g up

regu

latio

n

Up regulation No. of folds(and above) No.of spots

2 693 474 395 338 21

10 1615 1520 1025 850 375 1

Up regulationx Folds or above

4.1. Differential expression : Up regulation

A B C D E F

G H I J K

Mean normalized value of group-Control(Healthy person)

Mean normalized value of group 2 (P.falciparum diseased person) Ratio =

4.2. expression profile: Up regulation(8-10 folds up) -11 spots

Down RegulationNo. of folds(and above) No. of spots

2 83

3 48

4 37

5 28

7 13

10 11

15 5

30 2

No.

of s

pots

sho

win

g up

regu

latio

n

Down regulationx Folds or above

4.3.Differential expression : Down regulation

4.4.Expression profile: Down regulation (9-10 folds down) - 9 spots

A B C D E

F G H I

Mean normalized value of group-Control(Healthy person) Ratio =

Mean normalized value of group 2 (P.falciparum diseased person)

Hierarchical Cluster Analysis HCL Tree

It provides information about similarly expressed protein.

It helps in finding correlated protein

5.Cluster Analysis

6. Scatter plot