giving an effective presentation: using powerpoint and structuring a scientific talk susan mcconnell...
TRANSCRIPT
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Giving an effective presentation:Using Powerpoint and structuring a scientific
talk
Susan McConnellDepartment of Biological Sciences
Stanford University
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We may not be experts at public speaking, but we are all experts at
listening to talks
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What do you want from a talk?
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Here are some of the things many listeners
want from a talk:
CONTENT
Conveys new information Poses an interesting question Conveys how people in other f ields think Describes important ideas Novel discovery
CLARI TY AND ORGANI ZATION
Understandable Avoids jargon Uses clear and simple visual aids Well organized Enables me to catch up if I space out Doesn’t run over time
STYLE AND DELIVERY
Keeps me awake Varies voice Conveys enthusiasm Doesn’t stay in one place Friendly and approachable
EXPERTISE
Credible I nspires trust and conf idence Answers questions clearly
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This presentation focuses solely on ways of using Powerpoint and organizing a talk to
achieve:
CONTENT
Conveys new information Poses an interesting question Conveys how people in other f ields think Describes important ideas Novel discovery
CLARI TY AND ORGANI ZATION
Understandable Avoids jargon Uses clear and simple visual aids Well organized Enables me to catch up if I space out Doesn’t run over time
STYLE AND DELIVERY
Keeps me awake Varies voice Conveys enthusiasm Doesn’t stay in one place Friendly and approachable
EXPERTISE
Credible I nspires trust and conf idence Answers questions clearly
![Page 6: Giving an effective presentation: Using Powerpoint and structuring a scientific talk Susan McConnell Department of Biological Sciences Stanford University](https://reader036.vdocuments.us/reader036/viewer/2022062304/56649d845503460f94a6a4df/html5/thumbnails/6.jpg)
What do you think of the following slide?
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Emk1 knockdown inhibits lumen formation in MDCK cells:
-RT-PCR: EMK1 is effectively knocked down in MDCK cells 24 hours after transfection with P-SUPER (control) or P-SUPER-siEMK1 plasmid; knockdown confirmed on the right with antibodies to EMK1.
- Collagen overlay assay: cells cultured 24 h on collagen I before being overlaid with additional collagen on the apical surface, analyzed 24 h later. Note the lack of lumen in EMK1-KO cultures.
- Ca switch: control or EMK1-KO cells were plated in low Ca medium 24 h upon transfection with pSUPER or pSUPER-KO. After 12 h, cultures were switched to normal medium for 24 h. Transmission EM of cells sectioned perpendicular to the substratum shows lack of microvilli in EMK1-KO cells.
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Is this better?
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Emk1 knockdown inhibits lumen formation in MDCK cells
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Not much.
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Powerpoint basics:
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Powerpoint basics:1. What font to use
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Powerpoint basics:1. What font to use
Use a Sans Serif font:
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Powerpoint basics:1. What font to use
Use a Sans Serif font:
This font is Arial.
This font is Comic Sans.
This font is Trebuchet.
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Powerpoint basics:1. What font to use
Use a Sans Serif font:
This font is Arial.
This font is Comic Sans.
This font is Trebuchet.
Serif fonts take longer to read…
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Powerpoint basics:1. What font to use
Use a Sans Serif font:
This font is Arial.
This font is Comic Sans.
This font is Trebuchet.
Serif fonts take longer to read…
This font is Times New Roman.
This font is Courier.
This font is Didot.
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Powerpoint basics:1. What font to use
Some fonts look super in boldface:
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Powerpoint basics:1. What font to use
Some fonts look super in boldface:
Arial vs. Arial bold
Comic Sans vs. Comic Sans bold
Trebuchet vs. Trebuchet bold
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Powerpoint basics:1. What font to use
Type size should be 18 points or larger:
18 point
20 point
24 point
28 point
36 point
* References can be in 14 point font
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Powerpoint basics:1. What font to use
AVOID USING ALL CAPITAL LETTERS BECAUSE IT’S REALLY HARD TO READ!
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Powerpoint basics:2. Color
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Powerpoint basics:2. Color
Dark letters against a light background work.
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Powerpoint basics:2. Color
Light letters against a dark background also work.
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Powerpoint basics:2. Color
Many experts feel that a dark blue or black background works best for talks in a large
room.
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Powerpoint basics:2. Color
Dark letters against a light background are best for smaller rooms and for teaching.
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Powerpoint basics:2. Color
Avoid red-green combinations because a large fraction of the human population is red-green
colorblind.
Lots of people can’t read this –and even if they could, it makes your eyes hurt.
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Powerpoint basics:2. Color
Other color combinations can be equally bad.
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Powerpoint basics:2. Color
View your slides in grayscale to ensure that there is adequate color contrast in each slide.
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Powerpoint basics:3. Layout
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Powerpoint basics:3. Layout
Every slide should have a heading.
Sentences are preferred if it’s possibleto make a statement.
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Powerpoint basics:3. Layout
Limit text blocks to no more than two lines each.
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Powerpoint basics:3. Layout
The reason for limiting text blocks to two lines is that when the text block goes on and on forever, people in the audience are going to have to make a huge effort to read the text, which will preclude them from paying attention to what you are saying. Every time you lose their focus, your presentation suffers!
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Powerpoint basics:3. Layout
Lists should contain no more than 3 items:
• Item 1• Item 2• Item 3
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Powerpoint basics:3. Layout
It is often effective to “unveil” your list one by one:
• Item 1• Item 2• Item 3
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Powerpoint basics:3. Layout
Avoid sublists!
• Item 1- Item 1a- Item 1b- Item 1c
• Item 2 - Item 2a - Item 2b• Item 3
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Powerpoint basics:3. Layout
Be generous with empty space.
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Powerpoint basics:3. Layout
If you try to cram too much into a slide, and place things too close to the sides, they can get cut off if you’re using a poor projector. In any case, the slide looks all cluttered and junky.
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Powerpoint basics:4. Style
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Powerpoint basics:4. Style
Try your best to include asimple image on every slide.
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Powerpoint basics:4. Style
Limit the number of items on each slide.
Each slide should make just one or two points!
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Powerpoint basics:4. Style
This is just too much.
Arrrgh!
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Powerpoint basics:4. Style
Here is a simple rule for showing figures and images:
If you’re not going to take the timeto explain it, get rid of it.
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Powerpoint basics:4. Style
Avoid fancy transitions between slidesunless you have a good reason.
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Powerpoint basics:4. Style
Here is a sensible use of a “wipe” transition:
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Powerpoint basics:4. Style
Here is a sensible use of a “wipe” transition:
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Powerpoint basics:4. Style
Don’t try to show too many slides.
Often, less is more.
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It’s very easy to use Powerpoint really badly
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Emk1 knockdown inhibits lumen formation in MDCK cells:
-RT-PCR: EMK1 is effectively knocked down in MDCK cells 24 hours after transfection with P-SUPER (control) or P-SUPER-siEMK1 plasmid; knockdown confirmed on the right with antibodies to EMK1.
- Collagen overlay assay: cells cultured 24 h on collagen I before being overlaid with additional collagen on the apical surface, analyzed 24 h later. Note the lack of lumen in EMK1-KO cultures.
- Ca switch: control or EMK1-KO cells were plated in low Ca medium 24 h upon transfection with pSUPER or pSUPER-KO. After 12 h, cultures were switched to normal medium for 24 h. Transmission EM of cells sectioned perpendicular to the substratum shows lack of microvilli in EMK1-KO cells.
![Page 49: Giving an effective presentation: Using Powerpoint and structuring a scientific talk Susan McConnell Department of Biological Sciences Stanford University](https://reader036.vdocuments.us/reader036/viewer/2022062304/56649d845503460f94a6a4df/html5/thumbnails/49.jpg)
It takes some work and forethoughtto use Powerpoint well
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It takes some work and forethoughtto use Powerpoint well
Let’s break down the previous slide into its minimum essential
components
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RT-PCR
Western
MDCK (kidney)cells
EMK1 / Par1 can be knocked down inMDCK (kidney) cells using siRNA methods
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RT-PCR
Western
MDCK cells
EMK1 / Par1 can be knocked down inMDCK (kidney) cells using siRNA methods
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MDCK cells form a lumenfollowing a change in extracellular [Ca++ ]
gp135 -catenin ZO-1
Side view of lumen
MDCK cells
Surface view from lumen
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MDCK cells form a lumenfollowing a change in extracellular [Ca++ ]
gp135 -catenin ZO-1
Surface view from lumen
Side view of lumen
MDCK cells
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gp135
Lumen formation is blockedin EMK1 knockdown cells
-catenin ZO-1
MDCK cells EMK1 knockdown
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EMK1 knockdown cells also fail to form microvilli
MDCK cells EMK1 knockdown
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EMK1 knockdown cells also fail to form microvilli
MDCK cells EMK1 knockdown
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The structure of a good talk: start broad, get specific, and end broad
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The structure of a good talk: start broad, get specific, and end broad
Start with the biggest questions and get progressively more specific
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A powerful tool in a talk is a “home slide”
Design and introduce a “home slide” that you’ll come back to at each major transition in your
talk.
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A powerful tool in a talk is a “home slide”
Now we’ll build an introduction and a home slide that puts the previous data into context.
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Our bodies are full of tubes
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Our bodies are full of tubes
digestive enzymes
Intestine
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How do cells become polarized and form a lumen?
digestive enzymes
Intestine
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MDCK cells are a model system for apolarized cell type (from the kidney)
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MDCK cells are a model system for apolarized cell type (from the kidney)
apical proteins
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MDCK cells are a model system for apolarized cell type (from the kidney)
apical proteins centrosome
![Page 68: Giving an effective presentation: Using Powerpoint and structuring a scientific talk Susan McConnell Department of Biological Sciences Stanford University](https://reader036.vdocuments.us/reader036/viewer/2022062304/56649d845503460f94a6a4df/html5/thumbnails/68.jpg)
MDCK cells are a model system for apolarized cell type (from the kidney)
apical proteins centrosome
tight junctions
![Page 69: Giving an effective presentation: Using Powerpoint and structuring a scientific talk Susan McConnell Department of Biological Sciences Stanford University](https://reader036.vdocuments.us/reader036/viewer/2022062304/56649d845503460f94a6a4df/html5/thumbnails/69.jpg)
MDCK cells are a model system for apolarized cell type (from the kidney)
apical proteins
microtubules
centrosome
tight junctions
![Page 70: Giving an effective presentation: Using Powerpoint and structuring a scientific talk Susan McConnell Department of Biological Sciences Stanford University](https://reader036.vdocuments.us/reader036/viewer/2022062304/56649d845503460f94a6a4df/html5/thumbnails/70.jpg)
MDCK cells are a model system for apolarized cell type (from the kidney)
apical proteins
microtubules
tight junctions
extracellular matrix
centrosome
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MDCK cells lose their polarity in low [Ca++]
low [Ca++]
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MDCK cells regain their polarityin normal [Ca++] and reform a lumen
normal [Ca++]
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MDCK cells regain their polarityin normal [Ca++] and reform a lumen
time
normal [Ca++]
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EMK1 (also known as Par1) is a serine-threonine kinase that regulates polarity in
many cells
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EMK1 (also known as Par1) is a serine-threonine kinase that regulates polarity in
many cells
EMK1 localizes to tight junctions in MDCK cells
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Questions addressed today:
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Questions addressed today:
• Is the kinase EMK1 essential for polarizing kidney cells?
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Questions addressed today:
• Is the kinase EMK1 essential for polarizing kidney cells?
• Is EMK1 important for lumen formation?
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Questions addressed today:
• Is the kinase EMK1 essential for polarizing kidney cells?
• Is EMK1 important for lumen formation?
• How do different tissues form different types of tubes?
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The middle is the meat of the talk…
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…but talks are delivered to audienceswith limited attention spans
Audience attention curve
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The middle is the meat of the talk
The middle is also the time at which the audience tends to zone
out
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Enabling the audience to tune back in
After going into depth, come back to your home slide to make
transitions
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Enabling the audience to tune back in
Nontechnical
Generaltechnical
Specialist
After going into depth, come back to your home slide to make
transitions
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Enabling the audience to tune back in
Nontechnical
Generaltechnical
Specialist
Let’s review “episode 1” (which we’ve already designed) and add a
home slide
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Questions addressed today:
• Is the kinase EMK1 essential for polarizing kidney cells?
• Is EMK1 important for lumen formation?
• How do different tissues form different types of tubes?
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RT-PCR
Western
MDCK cells
EMK1 / Par1 can be knocked down inMDCK (kidney) cells using siRNA methods
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gp135
Lumen formation is blockedin EMK1 knockdown cells
-catenin ZO-1
MDCK cells EMK1 knockdown
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EMK1 knockdown cells also fail to form microvilli
MDCK cells EMK1 knockdown
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EMK1 is required for cell polarization
Normal MDCK cells:
low [Ca++] normal [Ca++]
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EMK1 is required for cell polarization
EMK1 knockdown cells:
low [Ca++] normal [Ca++]
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Use your home slide repeatedly to build a theme over time and enable the audience to
catch up
home slide
Nontechnical
Generaltechnical
Specialist
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Over the course of the talk, you can progressively build a fairly complex model
final home slide
Nontechnical
Generaltechnical
Specialist
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EMK1 regulates microtubules andcell polarity in two steps
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Increasing the level of EMK1 can alterthe type of lumen formed in step 2
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The structure of a good talk: start broad, get specific, and end broad
Focus now on conclusions
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Audience attention increases as you signal the end of the talk – so avoid false
endings!
Audience attention curve
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End with the most specific conclusions then build back out to the “big picture”
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EMK1 regulates microtubules andcell polarity in two steps
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Increasing the level of EMK1 can alterthe type of lumen formed in step 2
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The type of lumen formed by epithelial cells varies among different tissues
digestive enzymes
Intestine Liver
bile
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EMK1 may enable cells to make differenttypes of tubes in different organs
digestive enzymes
Intestine Liver
bile
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Organizing a great talk
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Organizing a great talk
• Be smart about Powerpoint
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Organizing a great talk
• Be smart about Powerpoint
• Introductions should start broad then get specific
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Organizing a great talk
• Be smart about Powerpoint
• Introductions should start broad then get specific
• Think of your talk as consisting of episodes
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Organizing a great talk
• Be smart about Powerpoint
• Introductions should start broad then get specific
• Think of your talk as consisting of episodes
• Use a home slide to make transitions effectively
![Page 108: Giving an effective presentation: Using Powerpoint and structuring a scientific talk Susan McConnell Department of Biological Sciences Stanford University](https://reader036.vdocuments.us/reader036/viewer/2022062304/56649d845503460f94a6a4df/html5/thumbnails/108.jpg)
Organizing a great talk
• Be smart about Powerpoint
• Introductions should start broad then get specific
• Think of your talk as consisting of episodes
• Use a home slide to make transitions effectively
• Conclusions should start with specifics but end broadly
![Page 109: Giving an effective presentation: Using Powerpoint and structuring a scientific talk Susan McConnell Department of Biological Sciences Stanford University](https://reader036.vdocuments.us/reader036/viewer/2022062304/56649d845503460f94a6a4df/html5/thumbnails/109.jpg)
Is this all you need to knowto give a great talk?
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Is this all you need to knowto give a great talk?
CONTENT
Conveys new information Poses an interesting question Conveys how people in other f ields think Describes important ideas Novel discovery
CLARI TY AND ORGANI ZATION
Understandable Avoids jargon Uses clear and simple visual aids Well organized Enables me to catch up if I space out Doesn’t run over time
STYLE AND DELIVERY
Keeps me awake Varies voice Conveys enthusiasm Doesn’t stay in one place Friendly and approachable
EXPERTISE
Credible I nspires trust and conf idence Answers questions clearly
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No, but it’s a good first step!
CONTENT
Conveys new information Poses an interesting question Conveys how people in other f ields think Describes important ideas Novel discovery
CLARI TY AND ORGANI ZATION
Understandable Avoids jargon Uses clear and simple visual aids Well organized Enables me to catch up if I space out Doesn’t run over time
STYLE AND DELIVERY
Keeps me awake Varies voice Conveys enthusiasm Doesn’t stay in one place Friendly and approachable
EXPERTISE
Credible I nspires trust and conf idence Answers questions clearly
![Page 112: Giving an effective presentation: Using Powerpoint and structuring a scientific talk Susan McConnell Department of Biological Sciences Stanford University](https://reader036.vdocuments.us/reader036/viewer/2022062304/56649d845503460f94a6a4df/html5/thumbnails/112.jpg)
A great resource for additional information is:
The Craft of Scientific Presentations
by Michael Alley