ION TORRENT™ SEMICONDUCTOR SEQUENCING The power of the HID-Ion Personal Genome Machine® (HID-Ion PGM™) System for forensics is the ability to help solve cases that cannot be solved with traditional DNA profiling technologies. Though capillary electrophoresis remains the gold standard and most cost-effective option for forensic analysts working with short tandem repeats (STRs), the HID-Ion PGM™ System extends forensic analysis capabilities by increasing the ability to obtain DNA results from compromised samples or when additional investigative leads are required.

The HID-Ion PGM™ System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to virtually any laboratory or research facility. The system’s use of simple, natural sequencing chemistry eliminates the need for expensive optics and reduces complex chemistries to measure natural DNA extension. Direct, real-time sequencing detection enables you to go from sample to results typically in less than 24 hours.

“The biggest impact is that we will be able to combine a large number of genetic markers of different types in a single run analysis, including markers such as STRs and single nucleotide polymorphisms (SNPs), potentially even RNA that cannot be multiplexed with any other current technology. This will open up new possibilities, as various types of forensically relevant information can be obtained from a single sample in a single analysis.”DR. MANFRED KAYSER

DEPARTMENT OF FORENSIC MOLECULAR BIOLOGYERASMUS UNIVERSITY ROTTERDAM, THE NETHERLANDS

Get more information from your forensic samples using

APPLICATIONS

IDENTIFYING STRs• Helps identify a suspect’s repeat alleles

and unique sequence using CODIS and non-CODIS loci

IDENTITY-, LINEAGE-, AND PHENOTYPE-INFORMATIVE SNPs

• Use multiple types of markers to identify and describe the owner of the sample

• Identify samples from compromised DNA

“The HID-Ion AmpliSeq™ PGM™ panels already include ancestry inference markers that allow for refined estimates of an individual’s biogeographic ancestry. The high-throughput sequencing methodology holds promise for even more powerful forensic panels currently being developed.”DR. KENNETH KIDDYALE UNIVERSITY SCHOOL OF MEDICINE

MITOCHONDRIAL DNA SEQUENCING• Explore the mitochondrial genome of several

individuals concurrently using whole-genome sequencing, or efficiently match individuals with just the control region

• Missing persons• Disaster victim identification

“These results are extremely promising and are expected to significantly increase the random match probability for mtDNA analysis when evaluating highly compromised samples.”1

DR. WALTHER PARSONINSTITUTE OF LEGAL MEDICINEINNSBRUCK, AUSTRIA

Next-generation sequencing has the potential to vastly expand the capabilities of human identification laboratories, enabling simultaneous analysis of a wide range of forensically relevant genetic markers to help resolve challenging cases.

EXPANDED CAPABILITIES

IMPROVE YOUR INVESTIGATIVE LEADS • Helps indicate probable ancestry and physical

features of the sample owner

HAVE GREATER CONFIDENCE IN YOUR IDENTIFICATION RESULTS

• Enables confirmation matches with more types of markers—including STR, single nucleotide polymorphism (SNP), and mitochondrial markers—and allows for better discriminatory match probabilities

GET MORE INFORMATION FROM CHALLENGING SAMPLES

• Allows for additional results from fragmented and partially degraded DNA

AVOID REDUNDANCY AND GET QUICKER RESULTS• Concurrently interrogate several samples for

hundreds of markers with the same instrument, enabling time and cost savings in your workflow and quicker results

2 Life Technologies™ | Ion Torrent™ Semiconductor Sequencing for Forensics and Human Identification

Learn more about relevant

HID-ION PGM™ SYSTEM HID APPLICATIONSfor processing your casework samples

EXTRACTION QUANTIFICATION AMPLIFICATION STR PROFILE GENERATION

DATABASE SUBMISSION

TRADITIONAL FORENSIC WORKFLOW

EXTEND WITH THE HID-ION PGM™ SYSTEM

3Life Technologies™ | Ion Torrent™ Semiconductor Sequencing for Forensics and Human Identification

ANALYZEHIGHLY DEGRADED

OR TRACE DNA

Identity-informative solution

ANALYZE DNA MIXTURES MORE

EFFICIENTLY

Next-generation technology

ANALYZE REMAINS OR

MISSING PERSONS

Mitochondrialsolution

GENERATEMORE INVESTIGATIVE

LEADS

Ancestry-informative solution

ANALYZEFAMILIAL AND

KINSHIP CASES

Lineage-informative solution

For Research, Forensic, or Paternity Use Only. When used for purposes other than Human Identification, the instruments and software modules cited are for Research Use Only. Not for use in diagnostic procedures. © 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. CO119904 0914

Join the worldwide Ion Torrent™ community ationcommunity.lifetechnologies.com/community/applications/hid

A simple, modular workflow with minimal hands-on time allows you to quickly go from sample to identification.

• Quickly find answers to frequently asked questions and stay updated on new products

• Explore exciting new forensic applications and the publications that describe them

• See how our tools work with preloaded data in our virtual machine environment

• Share methods and data with colleagues

• Become a part of the early-access network

SHARE EXPERIENCES WITH COLLEAGUES ON THE ION COMMUNITY

For more information, go to lifetechnologies.com/hid-ngs or contact your sales representative.

1. Walther Parson, PhD, Sharon Wootton, PhD, Lisa Lane Schade, MHR. “The Future of Forensics Has Arrived: The Application of Next Generation Sequencing.” Forensic Magazine April/May 2013.

SAMPLE TO RESULTS IN LESS THAN 24 HOURS

EXTRACTSAMPLES

1 HR 7 HR 7 HR 3 HR 1 HR