genetic relationship and allozyme expression of ... papers/jtas vol. 28 (1) apr... · genetic...

PertanikaJ. Trop. Agric. Sci. 28(1): 59 - 65 (2005) ISSN: 1511-3701© Universiti Putra Malaysia Press

Genetic Relationship and Allozyme Expression of Insecticide Susceptible andResistant Helopeltis theivora Populations from Peninsular Malaysia

:SITI NOOR HAJJAR MD LATIP, ^ T A MUHAMAD 8c *TAN SOON GUAN1 Department of Plant Protection, Faculty of Agriculture,

Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysiadepartment of Biology, Faculty of Science and Environmental Studies,

Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

Keywords: Allozyme, H. theivora^ PAGE, insecticide resistance

ABSTRAK

Helopeltis theivora dikenali sebagai perosak bagi koko dan teh di Malaysia. Beberapa mekanisme gkerintangan serangga telah dicadangkan, contohnya penurunan kadar sensitiviti bagi kawasan sasaran,detoksifikasi metabolik pestisid dan pengurangan kadar penembusan atau translokasi bagi racun serangga.Elektroforesis gel poliakrilamida (PAGE) digunakan untuk mengenal pasti enzim metabolik yang terlibat dalamperkembangan kerintangan bagi Helopeltis theivora. Serangga dewasa Helopeltis theivora diperoleh daripadatiga populasi yang berlainan iaitu Bukit Cheeding (Banting, Selangor), Sg. Palas (Cameron Highlands) danMARDI (Cameron Highlands, Pahang). Dua puluh lima enzim telah diuji untuk menentukan polimorfisme dan8 enzim telah dikenal pasti terdapat di dalam Helopeltis theivora. Dendogram yang dihasilkan daripadaanalisis kluster mengumpulkan populasi Banting dan MARDI dalam satu kumpulan manakala populasi Sg.Palas dikluster dalam kumpulan tersendiri.

ABSTRACT

Helopeltis theivora is a known pest of cocoa and tea in Malaysia. Several mechanisms of insecticide resistancehave been proposed such as reduction in the sensitivity of target sites, metabolic detoxification of pesticides anddecreased penetration or translocation of pesticides. Polyacrylamide gel electrophoresis (PAGE) was used todetermine the metabolic enzymes involved in the development of resistance in Helopeltis theivora. Adults ofHelopeltis theivora were collected from three geographical locations namely Bukit Cheeding (Banting, Selangor),Sg. Palas (Cameron Highlands) and MARDI (Cameron Highlands, Pahang). In total, 25 enzymes were screenedfor polymorphisms and 8 enzymes were detectable in H. theivora. The dendogram resulting from the clusteranalysis grouped the infrequently sprayed Banting and the MARDI populations together while the intensivelysprayed Sg. Palas population clustered by itself.

INTRODUCTION

Helopeltis theivora is a known pest of cocoa(Entwistle 1972) and tea (Wilson 1999) inMalaysia. Insecticides have been used for thecontrol of the mirids as cocoa is grown widely inthis country. Dzolkhifli et al (1998) reported thedevelopment of resistance in the Sungai Tekam,Pahang and Serdang populations of this insectto 7-HCH, deltamethrin and cypermethrin.Several mechanisms of insecticide resistance havebeen proposed such as reduction in the sensitivityof target sites, metabolic detoxification ofpesticides and decreased penetration ortranslocation of pesticides. Among them,

metabolic detoxification was shown to play amajor role in insecticide resistance (Sun 1992).The metabolic enzymes involved are those withroles in oxidation (mixed function oxidase),reduction (gluthatione s- transferase) andhydrolysis (esterase) (Matsumura 1985).Organophosphate and carbamate resistances areassociated with increased esterase activity in avariety of insects including aphids (Takada andMurakami 1988; Abdel-Aal et al. 1992), Coloradopotato beetle (Anspaugh et al. 1995), cockroaches(Siegfried et al. 1990; Prabhakaran and Kamble1993), and mosquitoes (Pasteur et al. 1984;Raymond et al 1987). Insects use specific or

SITI NOOR HAJJAR MD LATIP, RITA MUHAMAD & TAN SOON GUAN

general esterases to accomplish detoxificationand sequestration of these groups of insecticidesand electrophoresis can play a major role inidentifying the enzymes involved (Devonshireand Moore 1982). Based on allozymeelectrophoresis, esterase was found to be involvedin insecticide resistance in the fruit fly, Drosophillabuzzati, from two different populations inAustralia (Barker 1994). Therefore the objectiveof this study was to determine the metabolicenzymes involved in the development ofresistance in H. theivora by using polyacrylamidegel electrophoresis (PAGE).

MATERIALS AND METHODS

Adults of Helopeltis theivora were collected fromthree geographical locations namely BukitCheeding (Banting, Selangor), Sg. Palas(Cameron Highlands) and MARDI (CameronHighlands, Pahang) {Fig. 1) in April 2002. Thesamples were kept at - 70°C and twenty individualsfrom each population were used forelectrophoresis. Individuals of H. theivora werehomogenized with 50ml distilled water. 7%polyacrylamide gel electrophoresis (PAGE) wascarried out with an initial current of not morethan 50mA at 230V for 3 hours until the

bromophenol blue dye reached the anodal endof the gel. In total, 25 enzymes were screenedfor polymorphisms. The gels were stained for a-esterase (a-EST, EC 3.1.1.1) and j3- esterase (j3-EST, EC 3.1.1.2), octanol dehydrogenase (ODH,EC 1.1.1.37), acid phosphatase (ACP, EC 3.1.3.2),malate dehydrogenease (MDH, EC 1.1.1.37),sorbitol dehydrogenase (SDH, EC 1.1.1.14),glucose 6-phosphate dehydrogenase (G6PDH,EC 1.1.1.44), leucine aminopeptidase (LAP, EC3.4.11.1), xanthine dehydrogenase (XDH, EC1.2.1.37), alpha-glycererophosphate dehydrogenase(a-GPDH, EC 1.1.1.8), hexokinase (HK, EC2.7.1.1 ), aldehyde oxidase (ALDOX, EC 1.2.3.1),phosphoglucose isomerase (PGI, EC 5.3.1.9),peptidase (PEP, EC 3.4.1.1), isocitratedehydrogenase (IDH-NADP, EC 1.1.1.42),superoxide dismutase (SOD, EC 1.15.1.1),glutamate oxaloacetate transaminase(GOT, EC2.6.1.1), acid phosphatase acid (ACP, EC 3.1.3.2),malic enzyme (ME, EC 1.1.1.40), alcoholdehydrogenase (ADH, EC 1.1.1.1), pyruvatekinase (PK, EC 2.7.1.40), lactate dehydrogenase(LDH, E.C 1.1.1.27), 3-Hydroxybutyratedehydrogenase (HBDH, EC 3.1.1.31), alkalinephosphatase (AKP, EC 3.1.3.1) and adenylatekinase (AK, EC 2.7.4.3) activities separately by

Fig. 1: Map showing sampling sites

60 PERTANIKAJ. TROP. AGRIC. SCI. VOL. 28 NO. 1, 2005

ALLOZYMES IN HELOPELTIS THEIVORA FROM PENINSULAR MALAYSIA

the procedures of Shaw and Prasad (1970) withsome modification. The POPGENE Version 1.31computer package of Yeh and Boyle (1999) wasused to calculate allelic frequencies, geneticdistance (D) and F-statistics (Nei 1978). Basedon the phenotypes observed for these eightenzymes in 20 samples from each of the threepopulations, alleleic frequencies were obtainedwhich were in turn used to calculate the Nei's(1978) genetic distance coefficients among thethree populations. The genetic distancecoefficients were then used to cluster thepopulations by using UPGMA (unweighted pairgroup method with arithmetic averaging).

RESULTS AND DISCUSSION

The results showed that of the 25 enzymesscreened, 8 enzymes were detectable in H.theivora. The 8 enzymes were a- and f}- esterase(EST), xanthine dehydrogenase (XDH), leucineaminopeptidase (LAP), glutamate oxaloacetatetransaminase (GOT), superoxide dismutase(SOD), glucose 6-phosphate dehydrogenase(G6PDH), aldehyde oxidase (ALDOX) andalkaline phosphatase (ALKP) (Table 1). Allelicfrequency data for the 11 loci that could bescored in H. theivora are presented in Table 2,except for /3-EST-2 that was monomorphic in allpopulations. The esterase loci showed differentallelic frequencies in three populations. a-EST-1showed a polymorphism in the Bantingpopula t ion . a-EST-2, and /J-EST-1 werepolymorphic in all populations, while a-EST-3was monomorphic in all populauons. XDH waspolymorphic only in the Banting population butwas monomorphic in the Sg. Palas and MARDI,Cameron Highlands populations. LAP and SODwere monomorphic in all populations while

G6PDH and ALDOX were polymorphic in allpopulations. GOT was polymorphic in theBanting population but was monomorphic inthe Sungai Palas and MARDI, CameronHighlands population. AKP was polymorphic inthe Banting and Sungai Palas, CameronHighlands population but monomorphic in theMARDI, Cameron Highlands population. Allelicfrequencies for the polymorphic loci rangedfrom 0.0625 to 0.9375 (Table 2). Values of F-statistics for Helopeltis theivora are presented inTable 3. The mean F , Ft and Rvalues of 0.0493,0.4822 and 0.4554 respectively, indicate thedifferentiation among the populations. The locishowing the highest Fst values had low values forgene flow (Table 3). The dendogram resultingfrom the cluster analysis (Fig. 2) grouped theBanting, Selangor and MARDI, CameronHighlands populations together while the Sg.Palas, Cameron Highlands population clusteredby itself. This clustering pattern could be due tothe frequent spraying of insecticides at Sg. Palas,Cameron Highlands over the past 5 years (PhilipBauer, unpublished). For the Banting, Selangorand MARDI, Cameron Highlands populations,insecticides were not frequently sprayed whencompared with the Sg. Palas, Cameron Highlandspopulation. Based on the Nei's genetic distancevalue, the populations from Banting and MARDI,Cameron Highlands are closely related. In insects,esterases are known to be involved in importantphysiological processes, including the catabolismof juvenile hormone (Shanmugavelu et al 2000),insecticide resistance (Prevec et al 1992; Morton1993; Mutero et al. 1994), digestion (Argentinaand James 1995) and reproduction (Karotamand Oakeshott 1993). Insect esterase genes haveshown high rates of intraspecific and interspecific

TABLE 1Enzyme names, abbreviations, enzyme codes (E.C.), number of loci,

enzymes structures and electrophoretic buffer systems

Enzyme E.C. Number Number of loci Buffer system used

Alpha-esterase (a-EST)Beta-esterase (/3-EST)Aldehyde oxidase (ALDOX)Superoxide dismutase (SOD)Glucose 6-phosphate dehydrogenase (G6PDH)Xanthine dehydrogenase (XDH)Alkaline phosphatase (AKP)Glutamate oxaloacetate transaminase (GOT)Leucine aminopeptidase (LAP)

3.1.1.13.1.1.21.2.3.11.15.1.11.1.1.441.2.1.373.1.3.12.6.1.13.4.11.1

321111111

PoulikPoulikPoulikPoulikPoulikTEB

PoulikTEMMTEMM

PERTANIKAJ. TROP. AGRIC. SCI. VOL. 28 NO. 1, 2005 61

SITI NOOR HAJJAR MD LATIP, RITA MUHAMAD & TAN SOON GUAN

TABLE 2Allele frequencies and sample sizes (N) for 11 loci in

Hehpeltis theivora from three different populations

Locus

o-EST-1

a-EST-2

a-EST-3

/3-EST-l

XDH

LAP

GOT

SOD

G6PDH

ALDOX

AKP

Allele

(N)110100(N)110100(N)10100(N)105100(N)102100(N)102100(N)102100(N)105100(N)102100(N)102100(N)102100

Banting

400.50000.500040

0.10000.700040

0.00001.000040

0.40000.600040

0.30000.700014

1.00000.000040

0.87500.125032

1.00000.000032

0.06250.937540

1.00000.000030

0.86670.1333

Sg. Palas, C.H

400.00000.000040

0,25000.750040

0.00001.000032

0.25000.750040

1.00000.000038

0.00001.000040

0.33330.666712

0.13890.86116

1.00000.000030

0.27270.727322

0.27500.7250

MARDI, C.H

360.00000.000022

0.00001.00004

0.50000.500040

0.00001.000010

1.00000.000010

1.00000.000036

0.00001.000018

1.00000.000020

0.20000.800032

1.00000.000034

1.00000.0000

*C.H= Cameron Highlands

Banting.Selangor

MARDI,Cameron Highlands

Sg. Palas, CameronHighlands

0.400 0.375 0.350 0.325 0.300 0.275 0.250

Fig. 2: Dendogram constructed by using UPGMA clustering of three populationso/H. theivora based on Nei's (1978) genetic distance coefficients



TABLE 3/^statistics values for the 12 loci of 3 populations of Helopeltis theivara

Locus

XDHLAPGOTSOD

G6PDHALDOX

AKP_-EST-l_-EST-2-EST-3

_-EST-l_-EST-2

Mean

F.s

1.0009******-0.14291.00000.4045******0.5752-0.82310.6407-1.0000-0.5205******

0.0493

K1.0000L00000.79831.00000.4208*****0.7827-0.73910.6825-0.2000-0.27661.0000

0.4822

K0.60871.00000.82350.57140.02731.00000.48850.04600.11640.40000.16041.0000

0.4554

0.16070.00000.05360.18758.9073*****0.26175.18061.89770.37501.30870.0000

0.2990

*Nm =Gene flow estimated from Fst = 0.25 (1-FJ /Fu

TABLE 4Nei's Unbiased Measures of Genetic Identity (above diagonal) and Genetic Distance

(below diagonal) (1978) for three different populations of H. theivara

Population Banting Sg.Palas, C.H Mardi, C.H

BantingSg. Palas, C.HMardi, C.H

0.44930.2573

0.6381******0.3428

077320.7097

variation. Gene duplication followed bydivergence of duplicated genes seem to beinvolved in the origin of at least part of thisvariability (Brady and Richmond 1992). Thestability of insecticide resistance was found to bethe result of modifications of the insect genome(Guillemaud et al 1998; Raymond et al 2001).In the sheep blowfly, Lucilia cuprina, there is afitness cost associated with an allele coding forresistance to diazinon. But, following theintensive use of diazinon, a modifier gene hadbeen selected in the field which completelysupressed this cost. As a result, resistant insectscarrying the modifier gene are equally fit as thesusceptible insects.

CONCLUSION

Our results showed that the genetic structure ofthe population at Sg. Palas could have beenaffected by the intensive applications ofinsecticide when compared to the infrequentlysprayed populations of Banting and MARDI,Cameron Highlands.

ACKNOWLEDGEMENTS

This work was supported by funds from theMinistry of Science, Technology and theEnvironment, Malaysia through IRPA(Intensification of Research in Priority Areas)Project No: 01-02-04-EA0101-54095: Developmentof Resistance in Cocoa Mirid, Helopeltis theivara.

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(Received: 25 February 2005)(Accepted: 19 December 2005)


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