general laboratory tools techniques and methods browse through these files to get familiar with...
TRANSCRIPT
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General Laboratory Tools Techniques and Methods
Browse through these files to get familiar with equipment, techniques and general
approaches to use in your laboratory work this semester.
This is your ‘toolbox’
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‘Toolbox’ content
General techniques- equipment use
Glassware glossary
Volume measurement
Balances
Error analysis
Excel for lab work
Heating
Melting Point
pH meters
Waste disposal
HOW TO: Determine concentration of
unknown in solution
Prepare solutions
Mixture separations
Identify molecular or ionic
species
Identify ions in solution
How to measure heat exchange
Measure how fast a chemical
reaction proceeds (rates of
reactions)
Make buffers and how to use
them
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General Techniques-Equipment Use
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Error AnalysisPROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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Glossary of Common Laboratory Equipment
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Excel use for lab work
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Data and ResultsPROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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Heating Equipment
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Hot PlatePROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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Bunsen BurnersPROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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pH meters
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Using pH Meters PROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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Volumetric glassware
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Volumetric Equipment and Techniques PROPERTIES
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HOW TODetermine the concentration of unknown species in a solution?
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A. Titration-based on aqueous reactions
Acid-base titrations
Complexometric titrations◦Back titration calculation
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TitrationPROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Don't showCompletion Button Label: Next Slide
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Using an EDTA solution to titrate standard calcium solutions, an unknown water sample and tap waterPROPERTIES
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B. Spectroscopy-based on light absorption properties
UV-Vis IRMSNMR
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Different kinds of spectroscopyPROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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HOW TO prepare solutions?
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Preparing solutionsPROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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B. Dilution of available solutions
Going form a high concentration
solution to a low concentration
solution
• Serial dilution
• Parallel dilution
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Dilutions PROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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HOW TO: separate the components in a mixture?
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Types of Separations
• Solid-Solid
• Solid-Liquid
• Liquid-Liquid
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SeparationsPROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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HOW TO: determine whether a solution is ionic or molecular?
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A. Solubility
IN polar and non-polar solvents
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B. Melting/Boiling Point
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Melting and Boiling Point Determination
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C. Conductivity
Does it conduct electricity?◦Conductivity meter
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Conductivity simulation PROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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HOW TO : identify ions in solution?
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A. Aqueous chemical reactions
Based on solubility properties
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Separating ions from a solution of unknown cationsPROPERTIES
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B. Flame test
What’s the emission spectra?
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Flame testPROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide
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C. Spectroscopy
Based on energy absorption properties
• UV-Vis
• IR
• MS
• NMR
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General Introduction to Spectrophotometry Simulations and Data collection PROPERTIES
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How do you determine the rate of chemical reactions?
HOW TO measure the ‘activity’ of a catalyst?
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Chemical Kinetics - Reaction Rates
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A. Example: progression of a reaction catalyzed by an enzyme
◦Spectroscopy
◦Reaction rates
◦pH
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HOW TO make and why to use Buffer solutions?
• Why do you need buffers?
• How do you prepare a buffer solution?
• How do you select a buffer?
• How does a buffer work?
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How to mix buffer components-calculationsPROPERTIES
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HOW TO measure the heat exchange in a chemical reaction?
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Calorimetry
• Constant-pressure calorimetry
• Constant-volume calorimetry
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CalorimeterPROPERTIESAllow user to leave interaction: AnytimeShow ‘Next Slide’ Button: Show upon completionCompletion Button Label: Next Slide