gen mlt240211 final
TRANSCRIPT
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Cytogenetic Techniques
Christeen RJ Pedurupillay
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Cytogenetics
The study of the structure ,function, &evolution of chromosomes in a
metaphase(usually)/ prophase of
mitosis
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What is Kayotype?
The characterization of the
chromosomal complement of anindividual or a species, including
number, form, and size of the
chromosomes
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Important for thediagnosis, treatment and prognostication
Cytogenetic Testing
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Objective
To detect the numerical and structural
abnormalities in
� Children with Dysmorphic features
� Children with Congenital defects
� Couple with Recurrent Pregnancy loss
� Couples with subfertility
� Patients with Hematological Malignancies
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Chromosome Banding
Techniques
Cytogenetic Banding Techniques:
� G-banding (Giemsa)
� R-banding (Reverse)
� C-banding (Centomeric)
� Q-banding (Quinacrine)
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Normal male
46,XY
Normal femaleNormal female
46,XX46,XX
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Chromosomal Study Methods
� Traditional karyotyping method
(cytogenetics)
� Advanced methods of chromosomal
analysis( molecular cytogenetics)
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Blood
Bone Marrow
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Sample Collection and Transport �Peripheral blood: 2-5ml
�Bone Marrow: 0.5-1.0ml
�Anticoagulant: Sodium Heparin (sterile vial)
�Transport: At room temperature (4ºC, do not freeze)
�Reach the lab within 3-4 hours (ASAP)
�Clinical details & suspected diagnosis
�Consent from the patient
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Traditional Karyotyping Method
1. Culture setup
2. Arresting Cell Division at Metaphase
3. Harvesting
4. Slide Preparation
5. Capturing and Analyzing
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Methods
Bone Marrow
24 hrs Culturing
Patient·s Sample
Harvesting
Slide preparation
Chromosome Analysing &
Karyotyping
Peripheral Venous Blood
72 hrs cult uring
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Culturing
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� Growth promoting culture medium
Contain ² Mitotic agents
² Nutrient and salts
� Sterile laminar flow cabinet
� CO2 cell culture incubator
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Harvesting
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Mitosis arrest at metaphase ( add Colcimid)
Hypotonic treatment of the cells ( add KCl)
- Swells the cells
- Allow well spread of chromosomes
- Lyse RBCs
Fixation of cells ( methanol and acetic acid)- Stop cell division & Hypertonic Swelling
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Slide Preparation
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� Affected by humidity, Temperature & Air flow
� Ensure to use ² Clean Slides
- Fixed cell suspension dropped on to slides
- Drying of Slides ( Chromosome Aging)
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Staining
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Air Dried Slides
Add- Tripsin Solution
Rinse withWater
Add ² Giemsa Solution
Rinse withWater
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GTG Banding Pattern
� Giemsa stain following digestion of chromosome
with trypsin yields series of lightly and darkly
staining bands
� Heterochromatic regions: AT-rich DNA,
transcriptionally inactive, stain more darkly
� Euchromatic regions: GC-rich DNA, moretranscriptionally active, appear as light bands due
to less condensed chromatin
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Normal male
46,XY
Normal femaleNormal female
46,XX46,XX
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Capturing
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Applied Imaging Cytovision
Automated Karyotyping System
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Analysing
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Chromosome gain
Chromosome loss
Structural Abnormalities
Ploidy Levels
Numerical Abnormalities
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Chromosome gain
eg: Trisomyshould be seen in �2 spreads to be clonal
Chromosome loss
eg: Monosomyshould be seen in � 3 spreads to be clonal
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Structural chromosome abnormalities
eg: Translocations, Deletions, Inversions,
Duplications
Ploidy Levels
eg: Hyperploidy, Hypoploidy
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47,XY,+21
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45,X
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86, XXX, -X, -6,-7,-9,-10,-11,-13,-14,-16,-18,+19,+19,-
20,+22,+marker x 2
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46,XX,del(5)(p13)
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46,XX,dup(4p)
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46,XX,t(14;21)
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Disadvantages of Traditional G-
Banding Techniques
� Cytogenetic abberation below the band
resolution for visualization
- Micro deletions- Cryptic translocation
� Exchange of similar size and banding
pattern cannot be distinguished
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Why Culture Failures?
� Careless Handling
� Unsterile equipments, tubes, tips
� Personal Contamination (Lab Coat, Gloves)� Careless timings
� Environmental Conditions ( CO2, Humidity,
temperature)
� Contamination of the reagents
� Rarely reagents conditions & expairy
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Molecular Cytogenetic
Techniques
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� Fluorescence in situ hybridization
� Multicolour fluorescent technologies
- Spectral Karyotyping- Multiplex-fish (M-FISH)
- Comparative genomic hybridization
(CGH)
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Analysis of Chromosomes and Genes
- Gene location
- Micro ² deletions
- Rearrangements
- Amplification status
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