gel doc xr+ with image lab

8/14/2019 Gel Doc XR+ With Image Lab

1/8

27

49

9

6

3

13 17

14

11

68

211

16

9

16

9

7 5

15

14

11

7

15

12

68

211

5

1512

11

68

216

5

15

11

8

2

11

2

5

2

3

18

11

9

8

8

1

1

7

13

17

14

8

2

17

3

44

15

9

1

8

5

15

13

8

5

4

2

1

8

99

7

15

11

6

2

16

9

7

15

14

6

4

7

2

5

3

7

3 11

2

3

1

5

12 3

6

3

18

9

6 3

15

15

5

8

16

13

1

11

11

Eortless and Accurate

Gel Imaging and Analysis

Molecular ImagerGel DocXR+Systems

Imaging


2/8

Fast, Automated, Reproducible Imagingor Perect Images Every Time

For more than two decades, the Molecular Imager

systems rom Bio-Rad have been widely recognized

and trusted high-quality imaging instruments. Whether

you perorm routine imaging o PCR products or protein

puriications, the Gel Doc XR+ imaging system will meet

your needs. Designed or ease o use and an ability

to support a wide array o applications, it is a perect

it or individual laboratories, multiuser core acilities,

or research on inectious diseases such as avian lu

and H1N1 lu. Quality standards o engineering and

manuacturing make the system adaptable to academic

or biopharmaceutical laboratories. Thousands o

researchers over the years have published Gel Doc XRsystem images in dozens o journals and established a

record o reliability and excellence or the system.

The Molecular Imager Gel Doc XR+ system enables

quick and easy visualization, documentation, and

analysis o nucleic acid and protein gels, blots, and

macroarrays with a ew clicks o the computer mouse.

It is a lexible and easy to use system that supports

luorescence and colorimetric detection methods. The

large imaging area accommodates a broad range o gel

sizes, rom large handcast polyacrylamide gels to small

ReadyAgarose gels. The Gel Doc XR+ system is the

ideal accompaniment to your PCR and electrophoresis

systems, enabling image analysis and documentation

o restriction digests, ampliied nucleic acids, and

genetic ingerprinting.

What the Gel Doc XR+ System Can Do for You:

Automated workflow from image to results

n Makes your gel imaging and analysis quick

and eortless

n Eliminates the need or training

n Allows any user to repeat the same worklow

in exactly the same way

Automated image capture

n Produces beautiul pictures o your gels

n Delivers quantitative analysis o your

protein and DNA samples

Automated image optimization

n Delivers image data that is always

optimized and reproducible

without imaging artiacts

Automated image analysis

n Produces reports with data

organized the way you want

n Minimizes time rom lab

work to presentations


3/8

Gel Doc XR+ System Applications

Nucleic Acid Protein Gel

Electrophoresis Electrophoresis Blotting

Ethidium bromide Coomassie Blue Colorimetric

SYBR Green Copper stain Qdots 525

SYBR Sae Zinc stain Qdots 565

SYBR Gold Flamingo uorescent gel stain Qdots 625

GelGreen Oriole stain CY2

GelRed Silver stain Alexa Fluor 488

Fast Blast DNA stain Coomassie Fluor Orange DyLight 488

SYPRO Ruby Fluoresce in

Krypton

SYBR Safe stain

Oriole stain

Qdot blot

Coomassie Brilliant B

R-250 stain


4/8

11

68

211

16

9

7 5

15

11

12

8

5

8

2

11

2

14

8

2

17

4

8

5

15

13

8

5

4

2

1

8

99

7

15

11

6

2

16

9

7

15

14

6

3

3

2

3

1

5

12 3

3

18

9

6 3

15

5

8

16

13

1

11

11

Gel Doc XR+ System Is Poweredwith Image Lab Sotware

Fast Results from a Completely

Automated Workflow

The Gel Doc XR+ system is controlled by Image

Lab sotware to automatically and reproducibly

generate gel images. Image Lab sotware is ast,

taking you rom gel to printed results in seconds.

Your results are visible with a single click o the

computer mouse.

Image Lab sotware eliminates the guesswork

in imaging. You wont have to perorm tedious

repetitive steps to ind the right ocus setting.

You wont have to guess which exposure

times will best visualize the bands o interest.

Image calculations and corrections are done

automatically or your application. Whether you

are working with protein gels, nucleic acid gels,

or your own customized imaging application,

the Gel Doc XR+ system will select the proper

settings or optimum detection conditions o the

stain or label.

You wont need to set aside time or training new

users; the system and the sotware are easy

to use and work together rom setup to inish.

Imaging and image analysis can become the

simplest work in the laboratory.


5/8

Simple and Reproducible Image Capture

Place your gel on the Gel Doc imagers sample tray and

run your protocol: Your work is done in just one step.

Design and save protocols or the imaging steps in

your experiments, and Image Lab sotware will run theprotocols exactly the same way every time. The protocol

eature negates variability in results due to dierent people

operating the imaging system. When a new project is related

to an existing one, you can reuse the previously created

protocol by revising its parameters and renaming it. Image

Lab sotware lets you devote your valuable time to research

and discovery instead o to wondering i you used your

imaging system properly.

Results and Reports

In addition to printing a picture o your gel or your records, Imag

Lab sotware creates and prints reports o your experimental

data. Any part o the report can be copied into popular documen

processing applications such as Adobe Acrobat and Microsot

Word, PowerPoint, or Excel iles. To include a 3-D view o your

gel, copy it using the Snapshot tool, and paste it into your

presentation slide. High quality, good-looking reports are easy toproduce with the combined power o the Gel Doc XR+ system

and Image Lab sotware.

When an analysis parameter is changed, the results tables are

updated instantly to relect the new data.

Tutorials

With Image Lab sotware, you dont need previous imaging

experience to produce optimal gel images. Detailed tutorials are

accessible via the toolbar and start-up page to acquaint you with

all o the Image Lab sotware capabilities.

Lane proles depict band intensity and represent quantities o sample

components separated in a gel.

The image stays ocused at any zoom point.

Automated workfow or any application.

Three Unique Features

The power o Image Lab sotware stems rom three eatures

n Image Lab sotwares proprietary algorithms (patent pending

calibrate the system at setup or automatic control o ocus a

any zoom level and automatic correction o imaging artiacts

n Image Lab sotware perorms lat ielding corrections

speciically and consistently or each application

n Image Lab sotware saves the entire worklow (image capture

results, report) in a protocol ile. The protocols can be edited,

resaved, reused, and shared among multiple users

Tutorials or novice users.

Its that easy to use Image Lab to generate the data andreports that you need. Please look at the other tutorials ormore detailed explanations o other Image Lab eatures.


6/8

Molecular Biology Applications

The Gel Doc XR+ system can be used in multiple

workows. Gel electrophoresis remains an important tool

or quantitative analysis o complex protein and nucleic

acid samples. Applications include nucleic acid isolationor amplifcation or molecular cloning techniques, nucleic

acid quality evalution prior to quantitative PCR, gene

silencing, gene modulation, and gene expression. DNA gel

electrophoresis is also used or fne separations o nucleic

acid ragments or entire plasmids or analysis, cloning,

transection, environmental testing, and quality assurance

testing. Gel Doc XR+ systems are suitable or laboratories

engaged in RNAi analysis, molecular diagnostics,

epigenetics, pharmacogenomics, and orensic testing o

genomic DNA.

Alternative to UV illumination to better preserve DNA samples. Top,

serial dilu tions o EtBr-stained precision molecular mass ruler (Bio-Rad

Laboratories, Inc.) on agarose gel imaged with UV light; bottom, serially

diluted precision molecular mass ruler stained with SYBR Sae stain on

agarose gel imaged with XcitaBlueconversion screen.There is no loss

in sensitivity when a combination o SYBR Sae nucleic acid uorescent

stain and less harmul blue excitation is used instead o UV-excitable EtBr.

The image o the gel stained with SYBR Sae stain was taken using the

XcitaBlue conversion screen and a flter or SYBR Sae stain and GFP.

Less harmul detection methods preserve samples better and more

efciently or downstream uses such as cloning.

Safe DNA Imaging

Acquire images o DNA electrophoresis samples

without exposing samples or users to harmul UV

radiation by adding the XcitaBlue conversion screen

to your Gel Doc XR+ system. The screen enablesdetection o DNA with SYBR Sae stain and other blue

lightexcitable uorescent stains such as GelGreen and

SYBR Green. Images and analyzed data produced

by Gel Doc XR+ systems are oten incorporated into

sample preparation and sample quantitation workows.

Fluorescence,RU

18,000

16,000

14.000

12,000

10,000

8,000

6,000

4,000

2,000

0

Sample load, ng

51.2 25.6 12.8 6.4 3.2 1.62 0.8 0.4 0.2 0.1

SYBR Sae andXcitaBlue conversionscreen

EtBr and UV light

Ethidium Bromide vs. SYBR Safe DNA Stain Detection

PFGE gel stained with EtBr.

E. coli colony screening by PCR.

ReadyAgarose 96 Plus gel stained with EtBr.


7/8

Protein gels stained with silver stain (top) and Coomassie Blue

stain (bottom). Documentation o protein gels or lab notebooks or

sample analysis, including protein purity and concentration assessment

or dierential protein expression studies or to monitor gene modulation

products, is supported by the array o analys is tools in the Gel Doc XR+

system. The gel stained with silver stain shows salmon muscle, soybean,

and rat brain extracts, protein mixtures, andE. coli

extracts comparedwith Precision Plus Protein unstained standards.

Protein gels fuorescence-stained with Oriole stain (top) and

Flamingo fuorescent gel stain (bottom).Achieve higher levels

o sensitivity with uorescent stains to dierentiate proteins with low

expression. Resolve closely spaced spots or bands or protein profling,

quantitation, and characterization.

Capturing Gene Typing Results

Gel Doc XR+ systems are the imagers o choice or

pulsed ield gel electrophoresis (PFGE). Gel Doc XR+

system images generated rom CHEF Mapper XA

systems or PFGE have been used oten by PulseNet

International (a global network o health and oodregulatory agency laboratories coordinated by the

Centers or Disease Control and Prevention) or strain

typing and molecular epidemiology studies. Image

data and analysis o PFGE gels have contributed to

new indings in cancer research, ood saety, public

health, quality control, and genome mapping.

Images produced by the Gel Doc XR+ system have contributed

to new ndings in many elds.This gel shows a strain oSalmonella

identifed in a ood saety experiment.

Molecular ImagerGel DocXR+System


8/8

Life ScienceGroup

09-0689 1009 Sig 0308Bulletin 5838 Rev B US/EG

Bio-RadLaboratories, Inc.

Web site www.bio-rad.com USA 800 4BIORAD Australia 61 02 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil55 21 3237 9400Canada 905 364 3435 China 86 21 6426 0808 Czech Republic420 241 430 532 Denmark44 52 10 00 Finland09 804 22 00 France 01 47 95 69 65Germany089 318 84 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary36 1 455 8800 India 91 124 4029300 Israel03 963 6050Italy39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666 New Zealand0508 805 500Norway23 38 41 30 Poland48 22 331 99 99 Portugal351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 27 861 246 723Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland061 717 95 55 Taiwan 886 2 2578 7189 United Kingdom 020 8328 2000

Adobe Acrobat is a trademark o Adobe Systems Incorporated. Coomassie is a trademark o BASF Aktiengesellschat. Excel and Microsot are

trademarks o Microsot Corporation. GelGreen is a trademark o Biotium, Inc. SYBR is a trademark o Invitrogen Corporation. CHEF (U.S. patent

5,549,796, issued to Stanord University) is exclusively licensed to Bio-Rad Laboratories, Inc.

Specifcations

Automation Capabilities

Workow automated selection Application driven; user selected or recalled by a protocol

Workow automated execution Controlled by a protocol via application specifc setup or image area, illumination source, flter, analysis,

and reporting

Workow reproducibil ity 100% repeatabilit y via recallable protocols; rom image capture to quantitative analysis and reports

Autoocus (patent pending) Precalibrated ocus or any zoom setting or sample height

Image at felding** Dynamic; precalibrated and optimized or every application Autoexposure 2 user-defned modes (intense or aint bands)

Hardware Specications

Maximum sample size 28 x 36 cm

Maximum image area 25 x 26 cm

Excitation source Trans-UV and epi-white are standard (302 nm included, with 254 and 365 nm available as options);

optional trans white, sel-powered or conversion screen; optional XcitaBlue UV/blue conversion screen

Illumination control 3 modes (trans-UV, trans white, epi-white)

Detector CCD

CCD resolution (H x V) 1,392 x 1,040 pixels

Pixel size (H x V) 4.65 x 4.65 m

Cooling system Not applicable

Camera cooling temperature Not available

Filter holder 3 positions (2 or flters, 1 without flter)

Emission flters 1 included (standard), 3 optional

Dynamic range >3.0 orders o magnitude

Pixel density (gray levels) 4,096

Instrument size (L x W x H) 36 x 60 x 96 cmInstrument weight 32 kg

Operating Ranges

Operating voltage 110/115/230 V AC nominal

Operating temperature 1028C (21C recommended)

Operating humidity

gel doc xr+ with image lab

Documents