gastric fluid camembert
DESCRIPTION
This document is about the gastric fluid camembert.TRANSCRIPT
0 DAY (16-3)
PRIOR GASTRIC FLUID
time (min) SAMPLE CFU DILUTION Log10 average stdev5 A 6 1 6.00E+01 1.8 1.7 0.1
B 4 1 4.00E+01 1.6C
10 A 6 1 6.00E+01 1.8 1.7 0.1B 4 1 4.00E+01 1.6C
15 A 6 1 6.00E+01 1.8 1.7 0.1B 4 1 4.00E+01 1.6C
20 A 6 1 6.00E+01 1.8 1.7 0.1B 4 1 4.00E+01 1.6C
40 A 6 1 6.00E+01 1.8 1.7 0.1B 4 1 4.00E+01 1.6C
60 A 6 1 6.00E+01 1.8 1.7 0.1B 4 1 4.00E+01 1.6C
11 DAY (27-3)
PRIOR GASTRIC FLUID
time (min) SAMPLE CFU DILUTION Log10 average stdev5 A 30 4 3.00E+05 5.5 5.4 0.0
B 261 3 2.61E+05 5.4C
10 A 30 4 3.00E+05 5.5 5.4 0.0B 261 3 2.61E+05 5.4C
15 A 30 4 3.00E+05 5.5 5.4 0.0B 261 3 2.61E+05 5.4C
20 A 30 4 3.00E+05 5.5 5.4 0.0
CFU/cm2
CFU/cm2
B 261 3 2.61E+05 5.4C
40 A 30 4 3.00E+05 5.5 5.4 0.0B 261 3 2.61E+05 5.4C
60 A 30 4 3.00E+05 5.5 5.4 0.0B 261 3 2.61E+05 5.4C
21 DAY
PRIOR GASTRIC FLUID
time (min) SAMPLE CFU DILUTION Log10 average stdev5 A 269 5 2.69E+07 7.4 7.4 0.0
B 238 5 2.38E+07 7.4C
10 A 269 5 2.69E+07 7.4 7.4 0.0B 238 5 2.38E+07 7.4C
15 A 269 5 2.69E+07 7.4 7.4 0.0B 238 5 2.38E+07 7.4C
20 A 269 5 2.69E+07 7.4 7.4 0.0B 238 5 2.38E+07 7.4C
40 A 269 5 2.69E+07 7.4 7.4 0.0B 238 5 2.38E+07 7.4C
60 A 269 5 2.69E+07 7.4 7.4 0.0B 238 5 2.38E+07 7.4C
CFU/cm2
AFTER GASTRIC FLUID ALOA 0 day
CFU DILUTION Log10 average stdev time (min) log 10 stdev1 1 1.00E+01 1.0 1.0 0.0 0 1.7 0.11 1 1.00E+01 1.0 5 1.0 0.01 1 1.00E+01 1.0 10 1.0 0.01 1 1.00E+01 1.0 1.0 0.0 15 1.0 0.01 1 1.00E+01 1.0 20 1.0 0.01 1 1.00E+01 1.0 40 1.0 0.01 1 1.00E+01 1.0 1.0 0.0 60 1.0 0.01 1 1.00E+01 1.01 1 1.00E+01 1.01 1 1.00E+01 1.0 1.0 0.01 1 1.00E+01 1.01 1 1.00E+01 1.01 1 1.00E+01 1.0 1.0 0.01 1 1.00E+01 1.01 1 1.00E+01 1.01 1 1.00E+01 1.0 1.0 0.01 1 1.00E+01 1.01 1 1.00E+01 1.0
AFTER GASTRIC FLUID ALOA 11 day
CFU DILUTION Log10 average stdev time (min) log 10 stdev17 1 1.70E+02 2.2 2.2 0.1 0 5.4 0.020 1 2.00E+02 2.3 5 2.2 0.111 1 1.10E+02 2.0 10 1.7 0.622 1 2.20E+02 2.3 1.7 0.6 15 1.4 0.4
2 1 2.00E+01 1.3 20 1.2 0.23 1 3.00E+01 1.5 40 1.0 0.05 1 5.00E+01 1.7 1.4 0.4 60 1.0 0.01 1 1.00E+01 1.04 1 4.00E+01 1.61 1 1.00E+01 1.0 1.2 0.2
CFU/cm2
CFU/cm2
2 1 2.00E+01 1.32 1 2.00E+01 1.31 1 1.00E+01 1.0 1.0 0.01 1 1.00E+01 1.01 1 1.00E+01 1.01 1 1.00E+01 1.0 1.0 0.01 1 1.00E+01 1.01 1 1.00E+01 1.0
AFTER GASTRIC FLUID ALOA 21 day
CFU DILUTION Log10 average stdev time (min) log 10 stdev38 2 3.80E+03 3.6 4.1 0.5 0 7.4 0.0
322 2 3.22E+04 4.5 5 4.1 0.5170 2 1.70E+04 4.2 10 3.9 0.6
16 2 1.60E+03 3.2 3.9 0.6 15 4.5 1.010 3 1.00E+04 4.0 20 3.5 1.126 3 2.60E+04 4.4 40 3.4 1.1
8 2 8.00E+02 2.9 4.5 1.0 60 3.3 1.3134 2 1.34E+04 4.1
87 3 8.70E+04 4.92 2 2.00E+02 2.3 3.5 1.1
117 2 1.17E+04 4.115 3 1.50E+04 4.216 1 1.60E+02 2.2 3.4 1.146 2 4.60E+03 3.7
198 2 1.98E+04 4.37 1 7.00E+01 1.8 3.3 1.3
184 2 1.84E+04 4.372 2 7.20E+03 3.9
CFU/cm2
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
0η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
L. m
onoc
ytog
enes
(log
CFU
/cm
2)
pH
3/3 3/3 3/3 3/3 3/3 0/3
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
11η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
L. m
onoc
ytog
enes
(log
CFU
/cm
2)
pH
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
21η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
L. m
onoc
ytog
enes
(log
CFU
/cm
2)
pH
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
11η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
L. m
onoc
ytog
enes
(log
CFU
/cm
2)
pH
3/3 3/33/3
2/32/3 2/3
0 DAY
time (min) SAMPLE pH average stdev5 A 1.96 1.88 0.09 time (min)
B 1.89 0C 1.79 5
10 A 2.05 2.10 0.11 10B 1.95 15C 1.83 20
15 A 2.10 2.00 0.10 40B 2.00 60C 1.91
20 A 2.20 2.17 0.14 time (min)B 2.04 0C 1.93 5
40 A 2.34 2.17 0.15 10B 2.13 15C 2.04 20
60 A 2.43 2.25 0.16 40B 2.20 60C 2.13
11 DAY
time (min) SAMPLE pH average stdev5 A 1.75 1.71 0.05 time (min)
B 1.71 0C 1.66 5
10 A 1.80 1.76 0.05 10B 1.78 15C 1.71 20
15 A 1.85 1.81 0.05 40B 1.82 60C 1.75
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
0η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
L. m
onoc
ytog
enes
(log
CFU
/cm
2)
pH
3/3 3/3 3/3 3/3 3/3 0/3
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
11η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
L. m
onoc
ytog
enes
(log
CFU
/cm
2)
pH
20 A 1.90 1.86 0.05 time (min)B 1.87 0C 1.80 5
40 A 2.00 1.95 0.06 10B 1.96 15C 1.89 20
60 A 2.08 2.03 0.05 40B 2.04 60C 1.98
21 DAY
time (min) SAMPLE pH average stdev pH5 A 1.96 1.80 0.15 time (min)
B 1.77 0C 1.67 5
10 A 2.01 1.85 0.14 10B 1.81 15C 1.73 20
15 A 2.04 1.88 0.14 40B 1.84 60C 1.76
20 A 2.06 1.91 0.14 time (min)B 1.88 0C 1.79 5
40 A 2.12 1.98 0.12 10B 1.95 15C 1.88 20
60 A 2.15 2.02 0.11 40B 1.98 60C 1.94
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
21η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
L. m
onoc
ytog
enes
(log
CFU
/cm
2)
pH
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
11η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
L. m
onoc
ytog
enes
(log
CFU
/cm
2)
pH
pHaverage stdev
1.88 0.092.10 0.112.00 0.102.17 0.142.17 0.152.25 0.16
θετικά Fraser
3 from 33 from 33 from 33 from 33 from 30 from 3
pHaverage stdev
1.71 0.051.76 0.051.81 0.051.86 0.051.95 0.062.03 0.05
θετικά Fraser
3 from 33 from 33 from 32 from 32 from 33 from 3
pHaverage stdev
1.80 0.151.85 0.141.88 0.141.91 0.141.98 0.122.02 0.11
θετικά Fraser
3 from 33 from 33 from 33 from 33 from 33 from 3
0 DAY (16-3)
PRIOR GASTRIC FLUID
time (min) SAMPLE CFU DILUTION Log10 average stdev5 A 2 2 2.00E+02 2.3 2.2 0.2
B 1 2 1.00E+02 2.0C
10 A 2 2 2.00E+02 2.3 2.2 0.2B 1 2 1.00E+02 2.0C
15 A 2 2 2.00E+02 2.3 2.2 0.2B 1 2 1.00E+02 2.0C
20 A 2 2 2.00E+02 2.3 2.2 0.2B 1 2 1.00E+02 2.0C
40 A 2 2 2.00E+02 2.3 2.2 0.2B 1 2 1.00E+02 2.0C
60 A 2 2 2.00E+02 2.3 2.2 0.2B 1 2 1.00E+02 2.0C
11 DAY (27-3)
PRIOR GASTRIC FLUID
time (min) SAMPLE CFU DILUTION Log10 average stdev5 A 482 3 4.82E+05 5.7 5.7 0.0
B 536 3 5.36E+05 5.7C
10 A 482 3 4.82E+05 5.7 5.7 0.0B 536 3 5.36E+05 5.7C
15 A 482 3 4.82E+05 5.7 5.7 0.0
B 536 3 5.36E+05 5.7C
20 A 482 3 4.82E+05 5.7 5.7 0.0
CFU/cm2
CFU/cm2
B 536 3 5.36E+05 5.7C
40 A 482 3 4.82E+05 5.7 5.7 0.0B 536 3 5.36E+05 5.7C
60 A 482 3 4.82E+05 5.7 5.7 0.0B 536 3 5.36E+05 5.7C
21 DAY
PRIOR GASTRIC FLUID
time (min) SAMPLE CFU DILUTION Log10 average stdev5 A 202 5 2.02E+07 7.3 7.3 0.1
B 241 5 2.41E+07 7.4C
10 A 202 5 2.02E+07 7.3 7.3 0.1B 241 5 2.41E+07 7.4C
15 A 202 5 2.02E+07 7.3 7.3 0.1B 241 5 2.41E+07 7.4C
20 A 202 5 2.02E+07 7.3 7.3 0.1B 241 5 2.41E+07 7.4C
40 A 202 5 2.02E+07 7.3 7.3 0.1B 241 5 2.41E+07 7.4C
60 A 202 5 2.02E+07 7.3 7.3 0.1B 241 5 2.41E+07 7.4C
CFU/cm2
AFTER GASTRIC FLUID TSAYE 0 day
CFU DILUTION Log10 average stdev time (min) log 10 stdev3 1 3.00E+01 1.5 1.2 0.3 0 2.2 0.21 1 1.00E+01 1.0 5 1.2 0.31 1 1.00E+01 1.0 10 1.2 0.33 1 3.00E+01 1.5 1.2 0.3 15 1.1 0.21 1 1.00E+01 1.0 20 1.0 0.01 1 1.00E+01 1.0 40 1.0 0.02 1 2.00E+01 1.3 1.1 0.2 60 1.1 0.21 1 1.00E+01 1.01 1 1.00E+01 1.01 1 1.00E+01 1.0 1.0 0.01 1 1.00E+01 1.01 1 1.00E+01 1.01 1 1.00E+01 1.0 1.0 0.01 1 1.00E+01 1.01 1 1.00E+01 1.01 1 1.00E+01 1.0 1.1 0.22 1 2.00E+01 1.31 1 1.00E+01 1.0
AFTER GASTRIC FLUID 11 TSAYE day
CFU DILUTION Log10 average stdev time (min) log 10 stdev21 1 2.10E+02 2.3 1.9 0.5 0 5.7 0.0
2 1 2.00E+01 1.3 5 1.9 0.59 1 9.00E+01 2.0 10 1.8 0.5
20 1 2.00E+02 2.3 1.8 0.5 15 1.5 0.32 1 2.00E+01 1.3 20 1.3 0.65 1 5.00E+01 1.7 40 1.4 0.76 1 6.00E+01 1.8 1.5 0.3 60 1.0 0.02 1 2.00E+01 1.32 1 2.00E+01 1.31 1 1.00E+01 1.0 1.3 0.6
CFU/cm2
CFU/cm2
1 1 1.00E+01 1.01 2 1.00E+02 2.01 1 1.00E+01 1.0 1.4 0.71 1 1.00E+01 1.0
19 1 1.90E+02 2.31 1 1.00E+01 1.0 1.0 0.01 1 1.00E+01 1.01 1 1.00E+01 1.0
AFTER GASTRIC FLUID TSAYE 21 day
CFU DILUTION Log10 average stdev time (min) log 10 stdev35 2 3.50E+03 3.5 4.2 0.6 0 7.3 0.1
346 2 3.46E+04 4.5 5 4.2 0.6316 2 3.16E+04 4.5 10 4.0 0.6
23 2 2.30E+03 3.4 4.0 0.6 15 3.9 1.1114 2 1.14E+04 4.1 20 3.4 1.2334 2 3.34E+04 4.5 40 3.6 1.1
56 1 5.60E+02 2.7 3.9 1.1 60 3.3 1.2114 2 1.14E+04 4.1
71 3 7.10E+04 4.911 1 1.10E+02 2.0 3.4 1.2
108 2 1.08E+04 4.0177 2 1.77E+04 4.2
24 1 2.40E+02 2.4 3.6 1.179 2 7.90E+03 3.9
281 2 2.81E+04 4.49 1 9.00E+01 2.0 3.3 1.2
119 2 1.19E+04 4.198 2 9.80E+03 4.0
CFU/cm2
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
0η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
ΟΜ
Χ (lo
g CF
U/cm
2)
pH
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
11η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
ΟΜ
Χ (lo
g CF
U/cm
2)
pH
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
11η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
ΟΜ
Χ (lo
g CF
U/cm
2)
pH
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
21η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
ΟΜ
Χ (lo
g CF
U/cm
2)
pH
0 DAYpH
time (min) SAMPLE pH average stdev time (min) average5 A 1.96 1.88 0.09 0
B 1.89 5 1.88C 1.79 10 2.10
10 A 2.05 2.10 0.11 15 2.00B 1.95 20 2.17C 1.83 40 2.17
15 A 2.10 2.00 0.10 60 2.25B 2.00C 1.91
20 A 2.20 2.17 0.14B 2.04C 1.93
40 A 2.34 2.17 0.15B 2.13C 2.04
60 A 2.43 2.25 0.16B 2.20C 2.13
11 DAY
time (min) SAMPLE pH average stdev pH5 A 1.75 1.71 0.05 time (min) average
B 1.71 0C 1.66 5 1.71
10 A 1.80 1.76 0.05 10 1.76B 1.78 15 1.81C 1.71 20 1.95
15 A 1.85 1.81 0.05 40 1.95B 1.82 60 2.03C 1.75
20 A 1.90 1.95 0.05
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
0η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
ΟΜ
Χ (lo
g CF
U/cm
2)
pH
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
11η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
ΟΜ
Χ (lo
g CF
U/cm
2)
pH
B 1.87C 1.80
40 A 2.00 1.95 0.06B 1.96C 1.89
60 A 2.08 2.03 0.05B 2.04C 1.98
21 DAY
time (min) SAMPLE pH average stdev pH5 A 1.96 1.80 0.15 time (min) average
B 1.77 0C 1.67 5 1.80
10 A 2.01 1.85 0.14 10 1.85B 1.81 15 1.88C 1.73 20 1.91
15 A 2.04 1.88 0.14 40 1.98B 1.84 60 2.02C 1.76
20 A 2.06 1.91 0.14B 1.88C 1.79
40 A 2.12 1.98 0.12B 1.95C 1.88
60 A 2.15 2.02 0.11B 1.98C 1.94
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
11η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
ΟΜ
Χ (lo
g CF
U/cm
2)
pH
051015204060
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
21η ΗΜΕΡΑ
Χρόνος παραμονής σε γαστρικό υγρό (min)
ΟΜ
Χ (lo
g CF
U/cm
2)
pH
stdev
0.090.110.100.140.150.16
pHstdev
0.050.050.050.050.060.05
pHstdev
0.150.140.140.140.120.11