gabaa receptor-mediated modulation of...
TRANSCRIPT
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GABAA RECEPTOR-MEDIATED MODULATION
OF OSCILLATORY POTENTIALS IN THE ON-
AND OFF-RESPONSE OF THE FROG
ELECTRORETINOGRAM
Petia Kupenova, Elka Popova, Veselina Mihaylova Dept. Physiology, Medical University of Sofia
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0 100 200 300 400 500
-0.05
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0 100 200 300 400 500
-0.10
-0.05
0.00
0.05
0.10
0.150 100 200 300
-20
-10
0
10
20
Oscillatory potentials
ON OFF
ERG
b-wave d-wave
OPs
a-wave
-
MATERIALS AND METHODS
0 100 200 300
-20
-10
0
10
20
Filtering
OPs
• Ringer • Bicuculline 1M 10M 50M
-
0 10 20 30 400
100
200
300
400
b-mean
d_mean
Am
plitu
de
VTime (min)
0 100 200 300 400 500
-0.05
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0 100 200 300 400 500
-0.05
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0 100 200 300 400 500
-0.10
-0.05
0.00
0.05
0.10
0.15
0 100 200 300 400 500
-0.10
-0.05
0.00
0.05
0.10
0.15
0 100 200 300
-20
-10
0
10
20
0 200
-20
-10
0
10
20
Controls (Ringer solution)
RESULTS
5-10 min 35-40 min
ON OFF ON OFF
-
0 5 10 15 20 25 30 35 40 45 50 55 60 65
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
Re
lative
am
plit
ud
e
Time(min)
b-wave
d-wave
Rin; I=-4I=-3I=-4
Bic 1M
Ringer
Bic 10M
Bic 50M
I=-3I=-4
100 ms
100 microV
ON
OFF
GABA-A blockade (Bicuculline)
100 mis
100 microV
100 ms
5 microV
Ringer Bic 1µM Bic 50µM Bic 10µM
100 ms
5 microV
-
Individual OPs Abs and Rel change
0 1 2 3 4 5 6 7 80
1
2
3 OFF OPs_Rel change
A
Bic1muM
Bic10muM
Bic50muM
OP number
No
rma
lized
am
pli
tud
e +
+
0 1 2 3 4 5 6 7 80
1
2
3
Bic1muM
Bic10muM
Bic50muM
ON OPs_Rel change
No
rmali
zed
am
pli
tud
e
OP number
+
0 1 2 3 4 5 6 70
5
10
15
20
25 Rin.mean Bic1.mean
Bic10.mean
Bic50.mean
Rec.mean
OP number
Am
plitu
de
V
***
OFF-OPs
* *
***
*
**
0 1 2 3 4 5 6 7 80
10
20
30
Am
plitu
de
V
OP number
Rin
Bic1
Bic10
Bic50
Recov
ON-OPs
***
*
**
***
* * *
***
-
ON-OPs
Ringer
Bic 50µM
FFT
-
OFF-OPs
Ringer
Bic 50µM
FFT