1. First Biology on SANS2DWill accelerator source time-of-flight SANS deliver for structural biology? Cameron Neylon

2. 300 investment http://www.flickr.com/photos/smigol/2467443756 3. Five yearsso far http://www.flickr.com/photos/joelanman/366972076 4. 4 Q sin( / 2 ) 5. A range of wavelengths http://www.flickr.com/photos/7519597@N05/2466444043 6. to access more lengthscales http://www.flickr.com/photos/smithco/76749100 7. Wikimedia Commons Succinate_Dehydrogenase_1YQ3_and_Membrane.png Biology is complicated Wikimedia Commons MGMT BDNA_1T38.png 8. on a wide range William Crochot/NIST Wikimedia Commons, used with permission 9. of lengthscales Wikimedia Commons: Yeast_membrane_proteins.jpg 10. SANS is good for complex systems Wu et al., JBC, 2009, Manuscript M109.039537 11. So TOF SANS is great for biology? http://www.flickr.com/photos/thetruthabout/2842830409 12. But when we look for examples http://www.flickr.com/photos/suzanneandsimon/1561911861 13. Thats an overstatement but 14. Secretory IgA2 Bonner et al, J. BiolChem (2009) 284:5077 SANS data from LOQ 15. few examples of TOF-SANSused in model building 16. Why? http://www.flickr.com/photos/mattymatt/3017263513 17. Flux/Collection time 18. http://www.flickr.com/photos/fotoosvanrobin/3470106225 19. Background issues http://www.flickr.com/photos/god-country-history/1535244721 20. http://www.flickr.com/photos/akira_kev/2307577638 21. The sourcehttp://www.flickr.com/photos/scobleizer/2442984975 22. Low power http://www.flickr.com/photos/wheany/3200055138 23. more neutrons 24. The instrumentThe instrument http://www.flickr.com/photos/cellonaut/3225137280 25. Collimation L1 = 2 to 12 m, Sample at 19m from moderator, Sample to detector L2 = 2 to 12 m, Qmin~ 0.002 -1, = 1.5 to 12 by time of flight, Qmax~ 3 -1 STFC 26. STFC/Richard Heenan 27. STFC/Richard Heenan 28. Adjustable collimation without windowsSTFC 29. STFC/Richard Heenan 30. STFC/Richard Heenan 31. STFC/Richard Heenan 32. STFC/Richard Heenan 33. Two detectors for wide Q rangeSTFC 34. STFC/Richard Heenan 35. The data 36. Caveats Thick-walled moderator at low pressure Data from one detector onlyInstrument not fully aligned; collimation issues Issues with data reduction software slowing commissioning down 37. Lysozyme/D2O 8 mg.mL-1 3 hours L2 = 6m 6m collimation 38. Lysozyme/H2O 39. GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 -1 40. GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 -1one detector, one position 41. Problems Issues with background subtractionSystematic errors in reduction processInstrument scattering artefactsData reduction issues slowing optimization 42. Prospects Enormous simultaneous Q range Two-fold flux increase from sourceTwo-fold increase from alignment and collimation Three-fold from additional detector 43. ConclusionsLots of work to identify instrument configurations A versatile instrument for hierarchical systemsCompetitive collection times wrtbest instruments Real potential for structural biology 44. NIMROD NearandInterMediateRange OrderDiffractometerSTFC 45. Data from a mesostructured glassSTFCCurrently optimized Q-range of 0.02-1 to 50-1 46. 5 1040 70 STFC/Daniel Bowron 1-1 0.6-1 0.15-1 0.09-1 47. ProspectsWorld class SANS instrument for large samples High resolution data (but not verylow Q)Requires new data analysis techniques Combined data sets with differential labeling? 48. Reflectometers 49. Reflectometers 50. LMX ~2015 51. TS-2 and the biosciencesOptimizedinstrumentation on a unique source Powerful tools for hierarchical systemsCombining the best characteristics of instruments A view towards a future of long pulse sources? 52. http://www.flickr.com/photos/thetruthabout/2842830409 53. Acknowledgements SANS2d: Richard Heenan, David Turner, James Treadgold, Sarah Rogers, Ann Terry & Steve King Sample preparation: Luke Clifton Data reduction: The MANTID team UK Large Facilities Capital Fund STFC