federal centre of breeding research on cultivated plants

Avena genetic resources for quality in human consumption (AVEQ)Startup Meeting, Clermont Ferrand 2007

Federal Centre of Breeding Research on Cultivated Plants

Logistics in the project

Christoph U.Germeier

Logistics in the project

Christoph U.Germeier

Avena genetic resources for quality in human consumption (AVEQ)Startup Meeting, Clermont Ferrand 2007WP10: Data ManagementP1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12 P14 P15

P6

WP

8: A

nti

oxid

ants

NP-HPLC

WP

7: ß

-Glu

can

/Car

boh

ydra

tes

Enzyme

P2 P6

WP2: Working CollectionP0P1 P9 P10P5

Multipliers Provide some germplasmP8P6

WP

6: P

rote

in a

nd

Fat

NIRS

P5 P11

WP

1 C

oord

inat

ion

P1

P0

P0

P9

P5

P6

P3

P7

P15

P8

seedseed

samples

samples

samples

Kalibration

ProteinFat

P9P11

FIA

StarchFibre

P12 AVAP7

WP

5: M

ycot

oxin

- F

usa

riu

m ELISA PCRP6P15 P8

LC-MS/MSP4

WP3: Quality Field Experiments

P11P2 P1 P9

Nordic (N) E SW (S) E

P10P5

S

P8

WP4: Fusarium Field Experiments

P3

(N) E-Europe SE

P9P14

S

P8

seed

WP9: Frost Tolerance

S-Europe SE

P9

Field- Test

Climate C.P8

P8


Logisitics in the project

1.: Logistics in initial seed production (WP2 -> WP3,WP4, WP9WP2: P5 -> WP6: AveninWP2 -> WP9

3.: Logistics in Fusarium and mycotoxin analysis (WP4 -> WP5WP4 ->

WP8?)

2.: Logistics in quality analysis (WP3 -> WP6, WP7, WP8)



S-Europe SE

P9

Field- TestP8

Climate C.P8




P11P2 P1 P9


P10P5

S

P8


P3

(N) E-Europe SE

P9P14

S

P8

Total

12650

central distributor

a) central distribution?

?WP6: Avenin

plot P2 P11 P1 P5 P8 P9 P10size 2 2 2 4 2 2 2density 425 600 800 300 ? 800 500total 850 1200 1600 1200 1600 1000

plot P3 P14 P8 P9size 2 2 2 2density 400 600 ? 800total 800 1200 1600

plot P8 P9size 2 2density ? 800total 1600

Logistics in initial seed production

20072008

20082009



S-Europe SE

P9

Field- TestP8

Climate C.P8




P11P2 P1 P9


P10P5

S

P8


P3

(N) E-Europe SE

P9P14

S

P8

Total

12650

b) partner by partner distribution?

WP6: Avenin 20072008

20082009

Logistics in initial seed production

plot P2 P11 P1 P5 P8 P9 P10size 2 2 2 4 2 2 2density 425 600 800 300 ? 800 500total 850 1200 1600 1200 1600 1000

plot P3 P14 P8 P9size 2 2 2 2density 400 600 ? 800total 800 1200 1600

plot P8 P9size 2 2density ? 800total 1600


WP

6: P

rote

in a

nd

Fat NIRS

P5 P11 *verification

Kalibration

ProteinFat

P9P11 P5

Minerals


P11P2 P1 P9


P10P5

S

P8

P6

WP

8: A

nti

oxid

ants

NP-HPLC

AVAP7

Partner P7need AVSaccns 200g/sample 100

Logistics in quality analysis

Partner P5 P11method NIRS NIRSaccns 3300 500*g/sample 50 50

Partner P11 P9 P5descriptor Protein Fat Protein Fat Mineralssamples 300 50 50 300 100g/sample 20 20 20 20 ?

WP

7: ß

-Glu

can

/Car

boh

ydra

tes

Enzyme

P2 P6

StarchFibre

P6 P12

FIA

Partner P6 P6method FIA Starch/Fibreaccns 210 200g/sample ? 20

Partner P2 P6method total totalaccns 975g/sample 80 80

Partner P6need NP-HPLCaccns 325g/sample 20

20082009


WP

6: P

rote

in a

nd

Fat NIRS

P5 P11

*verification

Kalibration

ProteinFat

P9P11 P5

Minerals


P11P2 P1 P9


P10P5

S

P8

P6

WP

8: A

nti

oxid

ants

NP-HPLC

AVAP7


Logistics in quality analysis

Partner P5 P11method NIRS NIRSaccns 500* 3300g/sample 50 50

Partner P11 P9 P5descriptor Protein Fat Protein Fat Mineralssamples 50 300 300 50 100g/sample 20 20 20 20 ?

WP

7: ß

-Glu

can

/Car

boh

ydra

tes

Enzyme

P2 P6

StarchFibre

P6 P12

FIA

Partner P6 P12method FIA Starch/Fibreaccns 210 200g/sample ? 20

Partner P2 P6method total totalaccns 975g/sample 80 80


20092010


Summary: needed sample size in quality analysisNIRS Protein, Fat

per partnerNIRS 100gProtein 40gFat 40gg/sample 180g

ß-Glucan Starch, Fibreper partner

total 80gFIA ?Starch,Fibre 20gg/sample 100g

Antioxidants AVAper partner

NP-HPLC 20gAVA 100gg/sample 120g

From / To P5 P6 TotalP1 180 g 220* g 300 gP2 180 g 220 g 300 gP5 180 g 220 g 300 gP8 180 g 220 g 300 gP9 180 g 220 g 300 g

P10 180 g 220 g 300 gP11 180 g 220 g 300 g

* 100 + 120

From field to lab From lab to labFrom / To P9 P11 Total

P5 40 g 50 g 90 gFrom / To P12 P7 Total

P6 20 g 100 g 120 g

20082009



P3

(N) E-Europe SE

P9P14

S

P8

Partner P15 P6 P8 P8descriptorDON,T-2? DON,T-2? DON,T-2? Fusariummethod ELISA ELISA ELISA PCRsamples 500 100 500 100g/sample 20 20 20 20

WP

5: M

ycot

oxin

- F

usa

riu

m ELISA PCRP6P15 P8

LC-MS/MSP4

contrasting samples to one lab?similar samples to one lab?

Logistics in Fusarium and mycotoxin analysis

20082009

P6

WP

8: A

nti

oxid

ants

NP-HPLC

AVAP7



Partner P4descriptor multitoxin*method LC-MS/MSaccns 35samples 100g/sample 25

*HT-2 and T-2, nivalenol, deoxynivalenol, zearalenone, diacetoxyscirpenol, 3- and 15-acetyldeoxynivalenol


Summary: needed sample size in Fusarium analysis

From / To P6 P8 P15 TotalP3 40* (+120) g 20 g 45* g 105 (225) gP8 40 (+120) g 20 g 45 g 105 (225) gP9 40 (+120) g 20 g 45 g 105 (225) g

P14 40 (+120) g 20 g 45 g 105 (225) g* 20+20 * 20+25

From field to lab From lab to labFrom / To P4 Total

P15 25 25 g

From / To P7 TotalP6 100 g 100 g

per partnerplots 300+ 50ELISA 225+ 50?PCR 25LC-MS/MS 25g/sample 65

Antioxidants AVAper partner

NP-HPLC 20gAVA 100gg/sample 120g


Thanks for your attention

federal centre of breeding research on cultivated plants

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