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Faculty of Biotechnology and Animal Husbandry

LIST OF COURSES FOR EXCHANGE STUDENTS

ACADEMIC YEAR 2019/2020 Faculty of Biotechnology and Animal Husbandry

Course code (if applicable)

Course title Person responsible for the course

Semester (winter/ summer)

Level of studies (I, II or III)

Form Hours per form

ECTS points

1. ANIMAL EMBRYOLOGY Dr hab. inż. Tomasz Stankiewicz

Winter/ summer

II Lecture,

laboratory 30 4

2. ANIMAL OBSTETRICS Dr hab. inż. Tomasz Stankiewicz

Winter/ summer

II Lecture,

class 30 4

3. ANIMAL PHYSIOLOGY Dr hab. inż. Katarzyna Michałek

Winter/ summer

I Lectures, laboratory

30 4

4. BASIC MICROBIOLOGY Dr hab. inż. Karol Fijałkowski

Winter/ summer

I, II Lecture,

laboratory 30 4

5. BASICS OF ULTRASOUND DIAGNOSTICS

Dr hab. inż. Tomasz Stankiewicz

Winter/ summer

I Lecture,

laboratory 30 4

6.

BIOTECHNIK DER FORTPFLANZUNG BEI LANDWIRTSCHAFTLICHEN NUTZTIEREN

Dr hab. inż. Bogdan Lasota, prof. ZUT

Summer II Lecture, seminar,

laboratory 30 4

7. BIOTECHNOLOGY AND GENETIC ENGINEERING

Dr hab. inż. Arkadiusz Terman

Winter/ summer

I, II Lecture,

laboratory 30 4

8. CELL BIOLOGY Dr inż. Adam Lepczyński

Winter I, II Lecture,

laboratory 30 4

9. CELLULAR ENGINEERING IN ANIMAL REPRODUCTION


Winter/ summer

II Lecture,

laboratory 30 4

10. ENVIRONMENTAL TOXICOLOGY

Dr hab. inż. Agnieszka Tomza-Marciniak

Winter/ summer

I, II Lecture, seminar

30 4

11. FOOD AND NUTRITION IN RELATION TO HUMAN HEALTH

Dr hab. inż. Arkadiusz Pietruszka

Winter/ summer

I, II Lecture, seminar

30 4

12. FUNDAMENTALS OF LABORATORY DIAGNOSTICS


Winter/ summer

I, II Laboratory 30 4

13. GENERAL GENETICS Dr inż. Daniel Polasik Winter/ summer

I, II Lecture,

laboratory 30 4

14. GENETIC ENGINEERING METHODS

Dr hab. inż. Arkadiusz Terman

Winter/ summer

I, II Lecture,

laboratory 30 4

15. GENETIC MARKERS FOR FOOD QUALITY

Dr inż. Daniel Polasik Winter/summer I, II Lecture,

laboratory 30 4

16. GENOMICS Dr inż. Daniel Polasik Winter/ summer

I, II Lecture,

laboratory 30 4

Faculty of Biotechnology and Animal Husbandry

17. HUMAN GENETICS Dr inż. Daniel Polasik Winter/ summer

I, II Lecture,

laboratory 30 4

18.

IN VITRO AND IN VIVO METHODS IN TOXICOLOGY ASSESSMENT OF XENOBIOTICS


Winter/ summer

I, II Lecture,

class 30 4

19. INDUSTRIAL ENZYMOLOGY Dr inż. Radosław Drozd Winter/ summer

I, II Lecture,

laboratory 30 4

20. MEDICAL AND VETERINARY MICROBIOLOGY

Dr hab. inż. Karol Fijałkowski

Winter/ summer

I, II Lecture,

laboratory 30 4

21. METHODS OF MONITORING THE REPRODUCTIVE PROCESSES IN ANIMALS


Winter/ summer

II Lecture,

class 30 4

22. MOLECULAR BIOLOGY Dr hab. inż. Arkadiusz Terman

Winter/ summer

I, II Lecture,

laboratory 30 4

23. MOLECULAR DIAGNOSTICS Dr hab. inż. Arkadiusz Terman

Winter/ summer

I, II Laboratory 30 4

24. MOLECULAR MODELING OF ENZYMES Dr inż. Radosław Drozd

Winter/ summer

I, II Lecture,

laboratory 30 4

25. PROTEOMICS Dr inż. Agnieszka Herosimczyk

Winter/ summer

I, II Lecture,

laboratory 30 4

26. PROTÉOMIQUE Dr hab. inż. Malgorzata Ożgo

Winter/ summer

I, II Lecture,

laboratory 30 4

27. VACCINOLOGY Dr hab. inż. Karol Fijałkowski

Winter/ summer

I, II Lecture,

laboratory 30 4

FIRST DEGREE (BACHELOR)

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Field of study

Mode of study

Graduate's qualification

ECTS

Form of course credit

Code

Electives

Language

Elective group

Wymiana międzynarodowa

stationary

WBiHZ-1-03-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study

Educational profile -

Module

Course unit Animal Physiology

Field of specialisation

Administering faculty Katedra Fizjologii, Cytobiologii i Proteomiki

Form of instruction Semester ECTS Weight CreditCode Hours

laboratory course 1W, 2S 15 2,0 0,49 creditsL

lecture 1W, 2S 15 2,0 0,51 creditsW

Michałek Katarzyna ([email protected])Leading teacherDratwa-Chałupnik Alicja ([email protected]), Herosimczyk Agnieszka([email protected]), Lepczyński Adam ([email protected])Other teachers

PrerequisitesW-1 Basics of cell biology, biochemistry and animal anatomy.

Module/course unit objectivesC-1 Get knowledge about fundamental processes of the animal physiology.

C-2 To familiarise studenst with the laboratory techniques and equipment used in the study of animal physiology.

Course content divided into various forms of instruction Number of hours

T-L-1 Examination of the unconditioned reflexes: patellar reflex, plantar reflex, achilles reflex, pupillary lightreflex, corneal reflex. Analysis of a conditioned reflexes. Analysis of a reflec arc. 2

T-L-2 Observation of the muscle slides under the microscope. Mechanism of muscle contraction. 2

T-L-3 Hematocrit (Ht) estimation. Erythrocyte sedimentation rate (ESR) measurement. The influence ofcalcium ions for blood clotting process. 2

T-L-4 Hearing heart sounds. Observation of apex beat. Pulse rate measurement. Blood pressuremeasurement. The influence of physical exercises on pulse rate and blood pressure. 2

T-L-5Qualitative analysis of saliva content. Examination of the rate of digestion of starch by the salivaryamylase. The amylolytic properties of the pancreatic juice. The proteolytic properties of pancreaticjuice. The lipolytic properties of the pancreatic juice. Emulsification of lipid aggregates by the bile.

2

T-L-6Microscope observation of renal cortex and medulla. Examination of physical characteristics of urine ofdistinct animal species. The test for presence of glucose and ketones in the human urine. The influenceof water excess on diuresis and urine osmolality. Observation of aquaporin 2 (AQP2 ) in the kidney.

3

T-L-7Mechanism of inhalation and exhalation – the model of Donders. Measuring the vital capacity and itscomponents using spirometer. The influence of skin blood flow on its temperature. The influence ofwater evaporation and convection on human skin temperature.

2

T-W-1

Introduction to electrophysiology. Membrane potential. Action potential. Sodium potassium pomp.Structure and role of electrical synapse. Nerve cells and their function. Central and peripherial nervoussystem. Sympatic and parasympatic nervous system. The structure and function of a chemicalsynapse. Synaptic transmission. Components of a reflex: receptors – types and function; nerve centresand their properties; effectors. The definition of a reflex time. The mechanisms of conditioned reflexes.

2

T-W-2 Molecular mechanism of muscle contraction. Types of muscle contraction. Energetics of musclecontraction. Differences between physiological properties of skeletal and smooth muscles. 1

T-W-3Plasma and the cellular elements of blood. Homeostatic functions of blood. Blood cell production.Platelets and coagulation. Blood clotting process. Structure of the heart. Physiology of the cardiacmuscle. Cardiac muscle as the syncytium. The regulation of the heart beat and blood pressure. Factorsresponsible for blood flow and blood pressure. Cardiac cycle.

2

T-W-4Digestion in the oral cavity. The role of saliva. The components of gastric juice. Regulation of secretionof gastric juice. Ruminant digestive system.Digestion in duodenum. The components of pancreaticjuice. Regulation of secretion of pancreatic juice. The composition and functions of bile. Mechanisms ofabsorption in small intestine.

4

T-W-5Kidney function. Macro- and micro structure of the kidneys. Physical properties of normal urine ofvarious animals species. Pathological components of urine. Mechanisms of urine production. Thecomposition of primary and final urine. Glomerulal filtration, clearance, renal blood flow. Hormonalregulation of the renal function (AVP, ANP RAA).

4

Animal Physiology


T-W-6

Mechanisms of inhalation and exhalation. Respiratory gas exchange at lungs and tissues. Transport ofoxygen and carbon dioxide through the blood. Nervous regulation of breathing.Pulmonary volumes and capacities (total lung volume, lung volume, residual volume, expiratoryreserve volume, inspiratory reserve volume). The role of the thermoregulatory system in maintainingthe heat balance. Heat gain by the organisms. Heat loss effectors. Nervous and behavioural regulationof body temperature. Hypothermia, hyperthermia and heat – differences and definitions.

2

Student workload - forms of activity Number of hoursParcitipation to classes 15A-L-1

Self study content of the classes 10A-L-2

Preparing for the laboratory analysis 15A-L-3

Study of recommended literature 10A-L-4

Consultations 10A-L-5

Participation to lectures 15A-W-1

Self study content of the lectures 15A-W-2

Study of recommended literature 10A-W-3

Preparing for the test 8A-W-4

Consultation 10A-W-5

Writing the test 2A-W-6

Teaching methods / toolsM-1 Informative lectures with multimedia presentation.

M-2 Laboratory works.

Evaluation methods (F - progressive, P - final)S-1 Writing test.P

S-2 Assessment of student activity and preparing for classes.F

Designed learning outcomesReference to the

learning outcomesdesigned for the fields of

study

Reference tolearning outcomes

leading to thedegree of "inżynier"

Reference to thelearning outcomes

defined for theparticular areas of

education

Teachingmethods

Courseobjectives Course content Evaluation

methods

KnowledgeWM-WBiHZ_1-_??_W01Understanding of fundametal processes of the animalphysiology. Understandnig of physiological processes thatregulate body functions and the regulation of an organ systemfrom the molecular all the way to the whole animal level.

SkillsWM-WBiHZ_1-_??_U01Ability to describe the anatomy of different physiologicalsystems and their specific functions. Ability to describeinteractions between different organ systems. Ability to explainhow a whole animal physiological process occurs.

Other social / personal competencesWM-WBiHZ_1-_??_K01Teaching and explaining of fundamental processes of the animalsystem.

Outcomes Grade Evaluation criterion

KnowledgeWM-WBiHZ_1-_??_W01

2,0

3,0Student has a basic knowledge in the farme material program. Student undarstands the basic processes of the animalphysiology, the basic processes that regulate body functions. In the frame of expressing knowledge student makes a lot ofmistakes.

3,54,04,55,0

SkillsWM-WBiHZ_1-_??_U01

2,03,0 Student, with the help of teacher is able to describe the anatomy of different physiological systems and their specyfic

functions. Student, with the help of teacher is able to perfom laboratory exercises.3,54,04,55,0

Other social / personal competencesWM-WBiHZ_1-_??_K01 2,0

3,0 Student express moderate interest in participating in a verbal discussion with the teacher and a classmates during classes.

3,54,04,55,0

Required reading1. Hill RW, Animal Physiology, PALGRAVE MACMILLAN, 2012

2. Schmidt-Nielsen K, Animal Physiology: Adaptation and Environment, Cambridge University Press, 2002

3. Johnson BR, Ober WC, Garrison CW, Silverthor AC, Human Physiology: an integrated approach, Pearson Education., Boston, 2013

Supplementary reading1. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P, Molecular biology of the cell, Garland Science, New York, 2015

2. Silbernagl Stefan, Despopoulos A, Color atlas of physiology, 2009, 7 th Ed

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-04-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Basic Microbiology


Administering faculty Katedra Immunologii, Mikrobiologii i Chemii Fizjologicznej




Fijałkowski Karol ([email protected])Leading teacher

Other teachers

Prerequisites

W-1 Basic lab knowledge and skills.Ability to pipet, make solutions and dilutions and to execute protocols which require the use of sterile techniques.

Module/course unit objectivesC-1 The course aims are to provide a comprehensive theoretical and practical knowledge of animal microbiology.

Course content divided into various forms of instruction Number of hoursT-L-1 Information about working in microbiological laboratory 1

T-L-2 Bacterial structure, growth, cultivation 2

T-L-3 Methods of culturing bacteria isolated from different animals 2

T-L-4 Conditions of culturing microorganisms isolated from animals 2

T-L-5 Basics of mycological examination 2

T-L-6 Detection and identification of various kind of microorganisms isolated from animals 2

T-L-7 Identification of virulence factors produced by microorganisms isolated from animals 2

T-L-8 Molecular diagnostics of microorganisms isolated from animals 2

T-W-1 General bacteriological examination of material collected from animals 2

T-W-2 Detection and identification of various kind of microorganisms isolated from animals 2

T-W-3 Zootechnical microbiology 2

T-W-4 Zoonotic diseases 2

T-W-5 Microbiology of zootechnical products 2

T-W-6 Antimicrobial chemotherapy 2

T-W-7 Health and safety in microbiological laboratory 1

T-W-8 Antibiotic susceptibility of animal pathogens 2

Student workload - forms of activity Number of hoursParcipitation to classes 15A-L-1

Study of reccomended literature 15A-L-2

Preparing for the classes 15A-L-3


Parcypitation to lectures 15A-W-1

Study of reccomended literature 20A-W-2

Consultations 10A-W-3


Basic Microbiology

Teaching methods / toolsM-1 Informative lectures with multimedia presentations

M-2 Laboratory

Evaluation methods (F - progressive, P - final)S-1 Writing testP

S-2 Presentation of the projectF

S-3 Assessment of student activity and preparing for classesF



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_1-_null_W01The student can choose the appropriate techniques forexamination and identification of bacteria and fungi isolatedfrom animals

SkillsWM-WBiHZ_1-_null_U01The student uses skills on diagnostics of bacteria and fungi.

Other social / personal competencesWM-WBiHZ_1-_null_K01The student demonstrates responsibility and awareness of thedecisions made during the conduct of microbiological tests.


KnowledgeWM-WBiHZ_1-_null_W01

2,03,03,54,04,55,0

SkillsWM-WBiHZ_1-_null_U01

2,03,03,54,04,55,0

Other social / personal competencesWM-WBiHZ_1-_null_K01

2,03,03,54,04,55,0

Required reading1. L. M. Prescott, Microbiology, McGraw-Hill Science, USA, 2002

2. . L. Gyles, J. F. Prescott, J. G. Songer, C. O. Thoen C., Pathogenesis of Bacterial Infections in Animals 4th Ed, Blackwell Publishing, 20103. Winn W., Allen S., Janda W., Koneman E., Procop G., Schreckenberger P., Woods G., Color Atlas and Textbook of DiagnosticMicrobiology, Lippincott Williams and Wilkins, 2006, 5

Supplementary reading1. Bannister B., Gillwspie S., Jones J., Infection Microbiology and Management, Blackwell Publishing, London, 2006, 3

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-05-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Basics of Ultrasound Diagnostics


Administering faculty Katedra Biotechnologii Rozrodu Zwierząt i HigienyŚrodowiska




Stankiewicz Tomasz ([email protected])Leading teacher

Other teachers

PrerequisitesW-1 Basic knowledge of the topography of the internal organs and anatomy of animals.

W-2 The knowledge of physics and biophysics at the level of secondary school.

Module/course unit objectives

C-1 The aim of the course is to acquaint of students with ultrasound diagnostic imaging of animals and mastering the skill ofdescribing some organs of the body on the basis of the ultrasound images.

Course content divided into various forms of instruction Number of hoursT-L-1 Preparation of the patient and technical examination. 2

T-L-2 Assessment of functional status of the ovary on the basis of the ultrasound image. 3

T-L-3 Evaluation of uterus at different stages of ovarian cycle. 3

T-L-4 Evaluation of embryo and fetal development and parturition date calculation in selected species basedon the size of the fetus. 2

T-L-5 Imaging external and internal of male sex organs. 2

T-L-6 Imaging of physiological and pathological changes of thyroid on the example of selected mammalianspecies. 3

T-W-1 The achievements and the importance of diagnostic ultrasound in practice and science. 2

T-W-2 The construction, and working principle of ultrasound. 2

T-W-3 The concepts echogenicity in ultrasound. Echogenicity of various tissues and organs in the body. 2

T-W-4 Artefacts in ultrasound. Indications for ultrasound. The most common tests using ultrasound. 2

T-W-5 The use of ultrasound in animal reproduction. Examinations by per-rectum and abdominal wall. 4

T-W-6 Abdominal organs. Normal and pathological images based on selected species. 3

Student workload - forms of activity Number of hoursThe participation in the classes. 15A-L-1

Participation in the consultations. 10A-L-2

Preparing to the pass of the exercise. 35A-L-3

Participation in the classes. 15A-W-1

Participation in the consultations. 10A-W-2

The study of the professional literature. Preparing to pass lectures. 35A-W-3

Teaching methods / toolsM-1 The informative lecture with the use of multimedia techniques.

M-2 Activating methods (preparation and presentation of papers by students, discussion).

M-3 The demonstration, laboratory exercises (ultrasound examinations in the practice).

Basics of Ultrasound Diagnostics

Evaluation methods (F - progressive, P - final)S-1 The rating presentations prepared and delivered by students (teamwork) and engage in the discussion.F

S-2 The current control of the proper operation of students in laboratory classes.F

S-3 The final test covering a range of content of lectures and exercises.P



study





education

Teachingmethods


methods

Knowledge

C-1 S-1S-3

T-W-1T-W-2T-W-3

M-1M-2

WM-WBiHZ_1-_null_W01Student knows the possibilities of using the ultrasoundexamination in practice and describes the structure and functionof ultrasound apparatuses. Student lists the indications and themost common examinations by using ultrasonography. Studentknows the definition of echogenicity and presents echogenicityof selected tissues and organs in physiological and pathologicalconditions.

T-W-4T-W-5T-W-6

Skills

C-1 S-2

T-L-1T-L-2T-L-3

M-1M-3

WM-WBiHZ_1-_null_U01Student is able to use the right technique of ultrasoundexamination depending on the species, physiological status andpurpose of examination. The student will be able to apply theacquired knowledge and skills to the proper selection ofultrasound techniques and interpretation of ultrasound imagesin the evaluation of selected physiological and pathologicalconditions.

T-L-4T-L-5T-L-6

Other social / personal competences

C-1 S-1S-2

T-W-1 M-1M-2M-3

WM-WBiHZ_1-_null_K01After completing the course, the student will have a basis forstudying disciplines in further education in this field. Thestudent analyzes the problem of taking a group discussion.



2,03,0 The student knows the basic possibilities of using the ultrasound examination. Student describes only some structure and

function of USG apparatusses. He knows only in the basic level the definition of echogenicity of the tissues and organs.3,54,04,55,0


2,03,0 The student with help of the teacher is able to use the right technique of ultrasound examination and to identify of

particularly some tissue or organ.3,54,04,55,0


2,03,0 The student is aware of existing knowledge in further study of scientific disciplines related to ultrasound examination.

3,54,04,55,0

Required reading1. Gregory R. Lisciandro, Focused Ultrasound Techniques for the Small Animal Practitioner., Wiley-Blackwell, 2014

Supplementary reading1. Dominique Penninck, Marc-Andre d'Anjou, Atlas of Small Animal Ultrasonography., Wiley-Blackwell, 2008

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-07-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Biotechnology and Genetic Engineering


Administering faculty Katedra Genetyki i Ogólnej Hodowli Zwierząt




Terman Arkadiusz ([email protected])Leading teacher

Other teachers

PrerequisitesW-1 Basics of biotechnology methods

Module/course unit objectivesC-1 Get knowledge about biotechnology and genetic engineering

C-2 Get the practical experience in genetic analysis

Course content divided into various forms of instruction Number of hoursT-L-1 Isolation of genomic DNA came from different tissue 4

T-L-2 Enzymes in genetic engineering 2

T-L-3 Methods of nucleic acid detection. 2

T-L-4 Variations in PCR and their applications. 2

T-L-5 Molecular diagnostic in medicin. Analysis of polymorphisms in different gene in human. 5

T-W-1 Role of genes within cells, gene code and elements that control gene expression 4

T-W-2 Marker-assisted sellection for animal breedeng 3

T-W-3 PCR and its applications 2

T-W-4 Introductions and methods in gene therapy 2

T-W-5 Quantification and storage of nucleic acid 2

T-W-6 Construction of genomic library 2

Student workload - forms of activity Number of hoursParticipation in classes 15A-L-1

Self work with specialised publications 20A-L-2

Laboratury projet preparation 30A-L-3

Consultation 10A-L-4

Participation in lectures 15A-W-1

Self stady of recommended literature 25A-W-2

Preparing for the writting test 10A-W-3


Teaching methods / toolsM-1 Theoretical lectures

M-2 Laboratory works

Evaluation methods (F - progressive, P - final)

Biotechnology and Genetic Engineering

Evaluation methods (F - progressive, P - final)S-1 Writting testP

S-2 PresentationF



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_1-_??_W01Student get knowledge and can define genetic engineeringprocesses in biotechnology

SkillsWM-WBiHZ_1-_??_U01Students can use biotechnology methods relation with nuclearacid analyses

Other social / personal competencesWM-WBiHZ_1-_??_K01Explaining of basic of genetic engeneering



2,03,0 Studenst knows the basic information about genetic engineering using in biotechnology

3,54,04,55,0


2,03,0 Students know how to use the basic analysis of genetic endineering

3,54,04,55,0


3,0 Students can explain the basic information abou nuclear acid

3,54,04,55,0

Required reading1. Nair A.J., Introduction to biotechnology and genetic engineering, Infinity Science, 2011

2. Brown, Genomes 3, 2006

Supplementary reading1. Gibson, Muse, A promoter of genome science, 2009

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-08-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Cell Biology






Lepczyński Adam ([email protected])Leading teacherDratwa-Chałupnik Alicja ([email protected]), Herosimczyk Agnieszka([email protected]), Michałek Katarzyna ([email protected]),Ożgo Małgorzata ([email protected])

Other teachers

PrerequisitesW-1 Basicsof biochemistry and physiology

Module/course unit objectivesC-1 To obtain knowledge concerning structure and differentiation of distinct cells.

C-2 To gain insight into the specific functions displayed by cell membrane and various cellular organelles.

C-3 To develop the ability to think critically about issues in cell biology

Course content divided into various forms of instruction Number of hoursT-L-1 Types of cells and tissues. The interdependence between the cell structure and its function 4

T-L-2 Analysis of a cytoskeleton and cell cortex functions on the example of erythrocyte and sperm cells.Experimental destruction of the cell membrane of erythrocytes. 4

T-L-3 Localization, function and signal transduction of taste receptors. 3

T-L-4 Practical recognition of different stages of the processes of mitosis and meiosis 3

T-L-5 Visualization of leucocyte nucleus. 3

T-L-6 The influence of pH and temperature on enzymes activity. 3

T-W-1 Structure of cell membrane. Transport of small molecules across the cell membrane. 2

T-W-2 Principle of cell signaling. Major classes of cell-surface receptor proteins. 2

T-W-3 Structure and function of the cytoskeleton 2

T-W-4 Cell cycle and its regulation. 2

T-W-5 The compartmentalization of cells: rough and smooth endoplasmic reticulum, Golgi apparatus,mitochondrion, lysosome. Mechanism of vesicular transport. 2

Student workload - forms of activity Number of hoursParcitipation in laboratory classes 20A-L-1

Preparation to the laboratory classes 15A-L-2

Studying of recomended literature 15A-L-3


Participation in lectures. 10A-W-1

Study of the recommended scientific literature. 25A-W-2

Preparing for the writing test. 20A-W-3

Consultations. 5A-W-4

Teaching methods / tools

Cell Biology


M-2 laboratory





study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-1

T-W-1T-W-2T-W-3

M-1M-2

WM-WBiHZ_1-_??_W01Student should exhibit a general knowledge of the basicstructures and cell biology-related mechanisms in an eukaryotecell.

T-W-4T-W-5

Skills

C-1C-2C-3

S-2T-L-1T-L-2T-L-3 M-2

WM-WBiHZ_1-_??_U01- describe and carry out basic methods in cell biology- explain the theory behind the practical parts in the course andbe able to summarise and interpret experimental results

T-L-4T-L-5T-L-6


C-3 S-2

T-L-1T-L-2T-L-3T-L-4T-L-5T-L-6

M-2WM-WBiHZ_1-_??_K01Student creates an active attitude, has the ability to holisitcview on the facts in the field of the molecular biology

T-W-1T-W-2T-W-3T-W-4T-W-5



2,0

3,0- student has a basic knowledge in the frame of the material program- student exerts low attitude to knowledgein the frame of expressing knowledge student makes numerous mistakes

3,54,04,55,0


2,03,0 Student is able to perform basic analyses in the field of cell biology and with the help of the teacher comilate and interpret

their results3,54,04,55,0


3,0 Student is aware of biological processes complexity and recognizes the role of molecular mechanisms in maintaining thecellular homeostasis

3,54,04,55,0

Required reading1. Alberts B., Johnson A., Lewis J., Morgan D., Raff M., Roberts K., Walter P., Molecular biology of the cell, Garland Science, Taylor &Francis Group, 2015, 6th edition

Supplementary reading1. Lodish H., Berk A., Zipursky S.L., Matsudaira P., Baltimore D, Darnell J, Molecular Cell Biology, New York: W. H. Freeman, New York,2016, 8th edition2. Karp G., Cell and molecular biology. Concepts and experiments., WileyPlus, 2013, 7th edition

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-10-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Environmental Toxicology




lecturing course 1W, 2S 15 2,0 0,49 creditsA


Tomza-Marciniak Agnieszka ([email protected])Leading teacher

Pilarczyk Bogumiła ([email protected])Other teachers

PrerequisitesW-1 Knowledge of issues related to ecology and environmental protection.

Module/course unit objectivesC-1 To acquaint students with the toxicological characteristic of selected environmental pollutants.

C-2 To acquaint students with metabolism of toxins.

C-3 To acquaint students with factors influencing toxicity of xenobiotics.

C-4 To acquaint students with the basic mechanisms of functional disorders and morphological changes in selected organs andsystems.

Course content divided into various forms of instruction Number of hoursT-A-1 Toxicity testing of xenobiotics. Degrees of toxicity. Dose-response relationship. 4

T-A-2 Bioconcetration, bioaccumulation and biomagnification. Determination of BCF, BSAF and BMF (fordifferent types of ecosystems). 2

T-A-3 Toxicological characteristics of metals (Cd, Hg, Pb) and metalloids. Source of pollution, route ofabsorption, fate and mechanism of toxicity. MRLs. 2

T-A-4 Estimation of dietary daily intake of toxic substances. 2

T-A-5 Persistent organic pollutants (POPs) - toxicological characteristcs. 2

T-A-6 Estimation of dietary daily intake of selected POPs. 3

T-W-1Pollution and their fate in aquatic and terrestrial ecosystems.Classes of contaminants. Global transport of pollution. Factors determining the distribution ofpollutants in the environment. Models of pollutants spread in the environment.

2

T-W-2 Metabolism of xenobiotics. 2

T-W-3 Factors affecting the toxicity of xenobiotics (the physicochemical properties - dissociation, solubility,particle size, biological factors - age, sex, individual development). 2

T-W-4The biochemical effects of impurities (induction of detoxifying enzymes, and proteins capable ofbinding to heavy metal inhibition of cholinesterase, endocrine dysfunction, DNA adduct formation).Physiological effects of pollution (osmoregulation disorders, metabolic and neurological). The effects oftoxicological interactions (additive effects, toxicity potentiation, antagonism).

2

T-W-5Mutagenic and carcinogenic effects of xenobiotics. The impact of environmental pollution on thedevelopment of cancer. Types of carcinogens (genotoxic - working directly influence the metabolicactivation; epigenetic - promoters, cytotoxic compounds, modifiers of hormones, immunosuppressivecompounds).

2

T-W-6 Poisons of animal origin (poisons of insects, snakes, scorpions, fish). Symptoms and mechanism oftoxicity. 2

T-W-7 Toxicological characteristics of plastics. Toxicological classification of some preparations used inhouseholds. 3

Student workload - forms of activity Number of hoursparticipation in lectures 15A-A-1

Environmental Toxicology

Student workload - forms of activity Number of hourspreparation for test 20A-A-2

studying indicated literature 25A-A-3

participation in lectures 15A-W-1

preparation for test 15A-W-2

studying indicated literature 30A-W-3

Teaching methods / toolsM-1 Delivery method, lecture/presentation

M-2 Discussion

M-3 Explanation

Evaluation methods (F - progressive, P - final)S-1 testP

S-2 continuous assessmentF



study





education

Teachingmethods


methods

Knowledge

C-2 S-1T-W-2 M-1

M-3WM-WBiHZ_1-_null_W01The student discusses the toxins biotransformation and factorsaffecting the toxicity of xenobiotics.

T-W-3

C-1C-4 S-1

T-W-4T-W-5 M-1

M-2M-3

WM-WBiHZ_1-_null_W02Student discusses the mechanisms of functional disorders andchanges morphological organs and systems under of selectedtoxins.

T-W-6

C-1 S-1T-A-2T-A-3T-A-4T-A-5

M-1M-2M-3

WM-WBiHZ_1-_null_W03Student characterizes of selected xenobiotics.

T-A-6T-W-1T-W-7

Skills

C-1 S-2T-A-1

M-3WM-WBiHZ_1-_null_U01Student is able to calculate the LD50 for a specific subtancewith using different methods.


C-1C-2C-3

S-2

T-A-1T-A-2T-A-3T-A-4T-A-5T-A-6T-W-1

M-2WM-WBiHZ_1-_null_K01The student demonstrates an active engagement with solvingthe identified problems.

T-W-2T-W-3T-W-4T-W-5T-W-6T-W-7



2,03,0 The student describes the fate of toxins in the organism and explains the variability of the toxicity of specified substances.

3,54,04,55,0

WM-WBiHZ_1-_null_W02

2,03,0 Student describes the mechanisms of functional disorders and morphological changes in the organs and systems by the

action of selected toxins.3,54,04,55,0


2,03,0 Student correctly characterizes most of indicated xenobiotics.

3,54,04,55,0


2,03,0 Student is able to calculate the LD50 for a indicated xenobiotic, using as at least 2 methods.

3,54,04,55,0


2,03,0 The student demonstrates an engagement with solving the identified problems but does not engage spontaneously.

3,54,04,55,0

Required reading1. (Eds), General, Applied and Systems Toxicology, John Wiley and Sons, Online ISBN: 9780470744307, 2009, DOI:10.1002/9780470744307

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-11-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Food and nutrition in relation to human health


Administering faculty Katedra Hodowli Trzody Chlewnej, Żywienia Zwierząt iŻywności


seminars 1W, 2S 15 2,0 0,49 creditsS


Pietruszka Arkadiusz ([email protected])Leading teacher

Other teachers

PrerequisitesW-1 Knowledge on the subject in human physiology and biotechnology


C-1 Knowledge by a student chemical structure and properties of food components, their role in human nutrition, and changesduring technological processes

Course content divided into various forms of instruction Number of hoursT-S-1 Methods of determination of the basic nutrients in feed– introduction 2

T-S-2 Determination of dry matter, ash and crude protein 2

T-S-3 Determination of crude fiber, fiber fractions (NDF, ADL, ADF) and crude fat 3

T-S-4 Assessment of the nutritional protein value 3

T-S-5 Estimate chemical assessment of the nutritional protein value 3

T-S-6 Interpretation of the obtained results and conclusions 2

T-W-1 Human nutrition – basic terms 3

T-W-2 Lipids - role of fatty acids in human health 4

T-W-3 Carbohydrates and glicemic index. 3

T-W-4 Food Additives 4

T-W-5 Conclusions 1

Student workload - forms of activity Number of hoursParticipation in classes 15A-S-1

Reading specific literature 15A-S-2

Preparing for the exam 15A-S-3

Preparing projects in groups 15A-S-4


Reading specific literature 15A-W-2

Preparing for the exam 15A-W-3

Preparing for discussions 15A-W-4

Teaching methods / toolsM-1 Lecture

M-2 Didactic disscusion

M-3 Educational films

Food and Nutrition in Relation to Human Health

Evaluation methods (F - progressive, P - final)S-1 Short testF

S-2 Practical examF

S-3 ExamP



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_1-_??_W01Student get knowledge about the basic nutrients and theirimpact on human health.

SkillsWM-WBiHZ_1-_??_U01The student has the ability to evaluate food products and theircomposition for human development and health.

Other social / personal competencesWM-WBiHZ_1-_??_K01The student can explain the dangers associated with impropernutrition.



2,03,03,54,04,55,0


2,03,03,54,04,55,0


3,03,54,04,55,0

Required reading1. Julian E. Spallholz, Mallory Boylan, Judy A. Driskell., Nutrition: CHEMISTRY AND BIOLOGY, CRC Press, 1998, II, ISBN 0-8493-8504-0

2. Rudolf Steiner, Nutrition: Food, Health and Spiritual Development., Rudolf Steiner Press., 20063. Susan Allport, The Queen of Fats: Why Omega-3s Were Removed from the Western Diet and What We Can Do to Replace Them,University of California Press, 2006

Supplementary reading1. Geissler C, Powers H, Human Nutrition, Churchill Livingstone Elsevier, 2011

2. MJ, Lanham-New SA, Cassidy A, Vorster HH, Introduction to Human Nutrition, A John Wiley & Sons, Ltd., 2009

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-12-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Fundamentals of Laboratory Diagnostics






Other teachers

PrerequisitesW-1 physiology, anatomy

Module/course unit objectivesC-1 To acquaint students with the fundamental methods used in laboratory diagnostic

C-2 To acquaint students with the basic terms used in laboratory diagnostic.


T-L-1 Complete Blood Count (CBC) test. Semi-automated blood analysis.Evaluation of White Blood Cell 5

T-L-2 The urine analysis (Reader Urine Analyser). The physico-chemical and microscopic properties of theurine. Urine sediment analysis. 3

T-L-3 Biochemical tests. 2

T-L-4

The qualitative and quantitative methods in parasitology. Coproscopic techniques for detection andquantitative estimation of endoparasites. Microscopic Examination.The post-mortem parasitological examination: dissection, parasites isolation, preservation andexamination of collected samples.Detection of Trichinela in meat samples. Trichinoscopy and pool-sample digestion method.

10

T-L-5 Determination of selenium (Se) in biological samples 5

T-L-6 Laboratory diagnosis of cryptosporidiosis. 5

Student workload - forms of activity Number of hoursuczestnictwo w zajęciach 30A-L-1

an individual work, studying of literature 90A-L-2

Teaching methods / toolsM-1 laboratory

Evaluation methods (F - progressive, P - final)S-1 Continuous assessment of activities performed by student.F



study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-1

T-L-1T-L-2 M-1

WM-WBiHZ_1-_null_W01The student knows the basic terms used in laboratorydiagnostics.

T-L-3T-L-4

Skills

C-1 S-1T-L-1T-L-2T-L-3

M-1WM-WBiHZ_1-_null_U01The student is able to prepare samples of biological material,perform tests and interpret the results.

T-L-4T-L-5T-L-6

Fundamentals of Laboratory Diagnostics


C-1 S-1M-1WM-WBiHZ_1-_null_K01The student demonstrates responsibility for their own safety andothers.



2,03,0 The student knows only part of the required terms used in laboratory diagnostics.

3,54,04,55,0


2,03,0 The student is able to prepare the samples and perform tests, but needs the help of a teacher. Student presents the results,

but he/she can not assess them.3,54,04,55,0


2,03,0 The student demonstrates little responsibility for their own safety and others.

3,54,04,55,0

Required reading1. Pagana K., Pagana T., Mosby's Diagnostic and Laboratory Test Reference, Elservier Health Sciences, 2006

2. Garcia L., Practical Guide to Diagnostic Parasitology, American Society for Microbiology, 2009

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-13-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit General Genetics






Polasik Daniel ([email protected])Leading teacher

Other teachers

PrerequisitesW-1 Basics of molecular biology and biochemistry

Module/course unit objectivesC-1 Get knowledge about inheritance of traits

C-2 Training and practice in methods using in molecular genetics

Course content divided into various forms of instruction Number of hoursT-L-1 Genetic and physical mapping 2

T-L-2 Population genetics 3

T-L-3 DNA analysis methods and their practical use 10

T-W-1 History of genetics and terms using in genetics 2

T-W-2 Inheritance of quantitative and qualitative traits 5

T-W-3 Structure of DNA and chromosomes. 2

T-W-4 Genes and genetic code 2

T-W-5 Mutations and other sources of biodiversity 2

T-W-6 Genes expression and their regulation 2

Student workload - forms of activity Number of hoursParticipation to classess 15A-L-1

Solving and calculating problems 20A-L-2


Consultation 5A-L-4






Teaching methods / toolsM-1 Invormative lectures with multimedia presentations



General Genetics

Evaluation methods (F - progressive, P - final)S-2 Assessment of student activity and preparing for classesF



study





education

Teachingmethods


methods

Knowledge

C-1 S-1T-L-1T-L-2T-W-1T-W-2

M-1WM-WBiHZ_1-_null_W01Student defines the mechanisms of traits inheritance andindicates the sources of genetic variability

T-W-3T-W-4T-W-5T-W-6

Skills

C-2 S-2T-L-1T-L-2 M-2

WM-WBiHZ_1-_null_U01Student is able to solve genetic problems and gained experiencein basic molecular methods

T-L-3T-W-2


C-1C-2

S-1S-2

T-L-3T-W-1 M-1

M-2WM-WBiHZ_1-_null_K01Student is aware of benefits and dangers resulting fromachievements in modern genetics

T-W-5



2,03,0 Student knows the terms using in genetics as well as basic mechanisms of traits inheritance

3,54,04,55,0


2,03,0 Student solves easy genetic crosswords and gained experience in basic laboratory methods

3,54,04,55,0


2,03,0 Student gives a few practical examples of genetics applications

3,54,04,55,0

Required reading1. E. Passarge, Color Atlas of Genetics, Thieme Medical Publishers, 2012

2. H. Fletcher, I. Hickey, BIOS Instant Notes in Genetics, Garland Science, 2012

Supplementary reading1. J. E. Krebs, E. S. Goldstein, S. T. Kilpatrick, Lewin's GENES XI, Jones & Bartlett Publishers, 2014

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-14-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Genetic Engineering Methods







Other teachers

PrerequisitesW-1 Basics of molecular methods

Module/course unit objectivesC-1 Get knowledge about techniques used in genetic engineering

C-2 Get the practical experience in genetic analysis methods

Course content divided into various forms of instruction Number of hoursT-L-1 Using different mothods to extract nucleic acid. 3

T-L-2 Set up a PCR. 3

T-L-3 Restriction enzyme digestrin, analyze PCR product using agarose gel electrophoresis. 4

T-L-4 HRM - High- esolution melt curve analysis, RT-PCR, Real Time PCR, 5

T-W-1 Introduction: different methods used in genetic engineering and thair application. 2

T-W-2 DNA amplification methods including RT-PCR (reverse transcriptase), in situ PCR, mutational analysis. 4

T-W-3 PCR based mutation detection: SSCP, AS-PCR analysis, heteroduplex analysis, denaturing gradient gelelectiophoresis, 4

T-W-4 DNA microarrays ( DNA chips), sequencing, nucleotide enumeration. 4

T-W-5 Genetic engineering methods and ethical considetations 1












S-2 PresentationF

Genetic Engineering Methods



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_1-_??_W01Studenst has knowledge how to use modern molecular methods

SkillsWM-WBiHZ_1-_??_U01Student knows how to use genetic engineering methods

Other social / personal competencesWM-WBiHZ_1-_??_K01Explaining of basic of new methods use in genetic engineering



2,03,03,54,04,55,0


2,03,03,54,04,55,0


3,03,54,04,55,0




[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-15-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Genetic Markers for Food Quality







Other teachers

PrerequisitesW-1 Basics of genetics, physiology and molecular genetics

Module/course unit objectivesC-1 To familiarize students with possibility of genetic markers use in food analysis

C-2 Practical use of DNA analysis to assess food quality

Course content divided into various forms of instruction Number of hoursT-L-1 Methodological approach for food markers detection 2

T-L-2 Food fraud detection 4

T-L-3 DNA test for lactose intolerance 4

T-L-4 Tests for "supertaster" 5

T-W-1 Introduction, history, basic terms, markers classes, criteria of markers application 2

T-W-2 Genetic markers of taste and food preferences 2

T-W-3 Methods for GMO detection in food 2

T-W-4 Application of markers in food authentication 2

T-W-5 DNA barcoding and its application in food industry 2

T-W-6Genetic markers for:•fruit and vegetables quality•milk quality and quantity•different meat species quality

5




Preparing the multimedia presentation 10A-L-4


Parcipitation to lectures 15A-W-1






Genetic Markers for Food Quality

Teaching methods / toolsM-2 Laboratory works


S-2 Assessment of multimedia presentationF




study





education

Teachingmethods


methods

Knowledge

C-1 S-1S-2

T-W-1T-W-2T-W-3

M-1WM-WBiHZ_1-_null_W01Students indicates the need and practical application of DNAmarkers in food analysis

T-W-4T-W-5T-W-6

Skills

C-2 S-3T-L-1T-L-2T-L-3 M-2

WM-WBiHZ_1-_null_U01Student gained skills in the food analysis by use DNA markersand can define the dangers associated with consumption of non-authentic food

T-L-4T-W-3T-W-4


C-1C-2

S-1S-2

T-L-1T-W-4 M-1

M-2WM-WBiHZ_1-_null_K01Student is aware of needs and benefits of DNA markersapplication by the food analysis

T-W-5T-W-6



2,03,0 Student gives few examples of DNA markers application in food analysis

3,54,04,55,0


2,03,0 Student gained basic experience in food analysis by use DNA markers and gives few examples of food fraud

3,54,04,55,0


2,03,0 Student simply explains why DNA markers are usefull in food analysis

3,54,04,55,0

Required reading1. R. Blair, J. M. Regenstein, Genetic Modification and Food Quality: A Down to Earth Analysis, John Wiley & Sons, Ltd., 2015

2. D. Sun, Modern Techniques for Food Authentication, Elsevier, 2008

Supplementary reading1. I. S. Arvanitoyannis, Authenticity of Foods of Animal Origin, CRC Press, 2015

2. S. Sforza, Food Authentication Using Bioorganic Molecules, DEStech Publications, Inc., 2013

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-16-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Genomics







Other teachers

PrerequisitesW-1 Molecular biology and genetics

Module/course unit objectivesC-1 Get knowledge about genomes structure, sizes and evolution

C-2 Get knowledge and training in methods of genomes analysis

Course content divided into various forms of instruction Number of hoursT-L-1 Isolation of plasmids and restriction mapping 6

T-L-2 Isolation of mtDNA and D-loop polymorphism analysis 6

T-L-3 Practical application of genomic databases. 3

T-W-1 Introduction - history of genomics, fields, connection with other sciences 2

T-W-2 Size and structure of pro-, eukaryotic and organelle genomes with its comparison 2

T-W-3 Origin of new genes, ole of noncoding DNA 3

T-W-4 Diseases caused by genome architecture 2

T-W-5 Physical and genetic maps 2

T-W-6 Sequencing of genes and genomes 2

T-W-7 Methods in functional genomics 2




Preparing for classes 15A-L-4

Solving problems 10A-L-5








Genomics





study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-1


M-1WM-WBiHZ_1-_null_W01Student explains the issues related to the analysis of genomicsequences including genome projects and has knowledge in thearea of the functional and comparative genomics.

T-W-5T-W-6T-W-7

Skills

C-1C-2

S-1S-2

T-L-1T-L-2T-L-3T-W-2

M-1M-2

WM-WBiHZ_1-_null_U01Student perceives genome in holistic way regarding to itsstructure and function and acquired the ability to explore thedatabases containing deposited sequences and genomes data

T-W-5T-W-6T-W-7


C-1C-2

S-1S-2

T-L-3T-W-2T-W-3

M-1M-2

WM-WBiHZ_1-_null_K01Student creates an active attitude, has the ability to holisitcview on the facts and see the issues in a broader context

T-W-5T-W-6



2,03,0 Students knows basic terms in genomics, some structures and sizes of genomes and ways of genome sequencing

3,54,04,55,0


2,03,0 Student explains complexity of genomes, simply explores databases and indicates methods of genomes analysis

3,54,04,55,0


2,03,0 Student is aware of biological processes complexity and recognizes the role of genome within them

3,54,04,55,0

Required reading1. T.A. Brown, Genomes 3, Garland Science, 2006

2. A. Lesk, Introduction to genomics, Oxford University Press, 2012

Supplementary reading1. G. Gibson, S. Muse, A Primer of Genome Science, Third Edition, Sinauer Associates Inc., 2009

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-17-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Human Genetics







Other teachers

PrerequisitesW-1 Basics of Genetics

Module/course unit objectivesC-1 To get knowledge about inheritance of different traits, diseases and predispositions in human

C-2 Practical use of methods based on DNA analysis in human genetics

Course content divided into various forms of instruction Number of hoursT-L-1 DNA testing for chosen traits and predispositions in human 15

T-W-1 History of human genetics and milestones 2

T-W-2 Mitochondrial diseases 2

T-W-3 Model organisms in human genetics 2

T-W-4 The role of environment and genes in carcinogenesis 2

T-W-5 Ecogenetics 2

T-W-6 Genetic theories of aging 2

T-W-7 Genetics of sport performance 3



Preparing for the classess 15A-L-3











Human Genetics




study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-1

T-L-1T-W-2T-W-4

M-1WM-WBiHZ_1-_??_W01Description of genetic defects and predispositions in human andindication of practical knowledge application in human genetics

T-W-5T-W-6

Skills

C-1C-2

S-1S-2

T-L-1T-W-1 M-1

M-2

WM-WBiHZ_1-_??_U01Ability to interpret genetic data and use of acquired knowledgein daily life and in evaluation of the latest achievements in thefield of human genetics

T-W-3T-W-7


C-1C-2 S-2

T-W-1T-W-3 M-1

M-2WM-WBiHZ_1-_??_K01Awareness of the advantages and risks of the achievements ingenetics

T-W-4T-W-7



2,03,0 Student describes a few examples of genetic defects and predispositions in human and their inheritance

3,54,04,55,0


2,03,0 Student is able to interpret simple genetic data

3,54,04,55,0


3,0 Students is aware of the advantages and risks of the achievements in genetics on satisfactory level

3,54,04,55,0

Required reading1. Lewis R., Human Genetics, 11th Edition, McGraw-Hill Education, 2014

Supplementary reading1. Speicher M. R., Motulsky A. G., Stylianos E., Vogel and Motulsky's Human Genetics. Problems and Approaches., Springer, 2010

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-19-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Industrial Enzymology






Drozd Radosław ([email protected])Leading teacher

Other teachers

PrerequisitesW-1 Basic knowlage of chemistry, biochemistry and biophysic


C-1 The purpose of the course is to teach students about technologies of industrial enzymes manufacturing and characterize theadvantages of using enzyme preparations in the industry.

Course content divided into various forms of instruction Number of hoursT-L-1 Estimation of basic catalytical parameters of enzymes with invertase form S. cerevisiae as model 6

T-L-2 Production laccase from T.versicolor 4

T-L-3 Immobilization of alpha amylase on polysaccharides carriers 3

T-L-4 Starch conversion by immobilised amylolytic enzymes for biofuel production 2

T-W-1 Principles of enzymology 6

T-W-2 Methods of enzymes production for industrial applications 2

T-W-3 Strategies for improving enzymes for industrial application 2

T-W-4 Enzymes in food industry 2

T-W-5 Enzymes in biofuel production 2

T-W-6 Enzymes in environment protection 1

Student workload - forms of activity Number of hoursParticipation in laboratory classes 15A-L-1

Project preparation 15A-L-2

Reading and analysis of specialised literature 30A-L-3

Parcypitation in lectures 15A-W-1

Reading and analysis of specialised literature 30A-W-2

Analysis of scientific articles indicated by the teacher 15A-W-3

Teaching methods / toolsM-1 lectures

M-2 disscusion

M-3 laboratory lectures

M-4 preparation of project

Evaluation methods (F - progressive, P - final)S-1 Presentation of projectP

Industrial Enzymology



study





education

Teachingmethods


methods

Knowledge

C-1 S-1

T-L-1T-L-2T-L-3T-L-4T-W-1

M-1M-2M-3M-4

WM-WBiHZ_1-_??_W01Student has knowledge about importance, usefulness andapplication area, sources and methods of modification ofenzymes from various sources for use in industry


Skills

C-1 S-1


M-1M-2M-3M-4

WM-WBiHZ_1-_??_U01Students choose and apply appropriate tools for enzymecharacterisation, and its modification for further use in industry



C-1 S-1


M-1M-2M-3M-4

WM-WBiHZ_1-_??_K01Students understand importance of technical enzymes inmodern industry development




2,03,0 The report ofproject preparation and presentation

3,54,04,55,0


2,03,0 The project report preparation and presentation

3,54,04,55,0


3,0 The project report preparation and presentation

3,54,04,55,0

Required reading1. Wolfgang Aehle red., Enzymes in Industry: Production and Applications, Willey VCH, 2007, III

2. Allan Svendsen, Enzyme Functionality: Design, Engineering and Screening, 2004

3. Christoph Wittmann i Rainer Krull red., Biosystems Engineering I: Creating Superior Biocatalysts, Tom 1, Springer, 2010

4. Girish Shukla i Ajit Varma, Soil Enzymology, Springer, 2011

Supplementary reading1. Athel Cornish-Bowden, Fundamentals of Enzyme Kinetics, Portland Press, Londyn, 2002, III

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-18-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit In vitro and in vivo Methods in ToxicologicalAssessment of Xenobiotics








PrerequisitesW-1 no requirements

Module/course unit objectivesC-1 To acquaint students with the in vivo and in vitro methods used in assessing the toxicity of xenobiotics.

C-2 To acquaint students with the mechanisms of action of toxic substances and their metabolism.

C-3 To acquaint students with the computational methods in toxicity, exposure and risk assessment.

Course content divided into various forms of instruction Number of hoursT-A-1 Metabolism of xenobiotics. 2

T-A-2 The mechanisms of toxicity. 2

T-A-3 Methods for determining the median lethal dose/concentration (LD50 i LC50). 3

T-A-4 Calculation methods in the toxicity assessment. Exposure and risk assessment. Determination ofNOAEL, LOAEL, LoAL and RfD. 3

T-A-5 Toxicological evaluation of raw materials and cosmetic products. 2

T-A-6 Alternative methods in ecotoxicological studies. 3

T-W-1 The use of animals in toxicometric research. The main organizations promoting alternative methods inthe world. Database of in vitro techniques used in toxicology. 2

T-W-2 Use of in vivo tests in evaluation of the toxicity of chemicals. Types and directions of toxicologicalresearch. 2

T-W-3 Acute toxicity - classic and alternative methods. 4

T-W-4 Repeated dose toxicity. The methods used in assessing the genotoxicity, carcinogenicity,neurotoxicity, effects on reproduction, fertility and offspring. 3

T-W-5Evaluation of toxicity of a compound based on the relationship between the chemical structure andbiological activity (structure-activity relationship). Factors affecting the toxicity. Genetic factorsincreasing the sensitivity to chemical compounds.

2

T-W-6 Chemical safety. The most important rules governing the issue of chemical safety. The classificationand labeling of chemicals. 2



preparation for test 15A-A-3




In vitro and in vivo Methods in Toxicological Assessment of Xenobiotics

Teaching methods / toolsM-1 Delivery method, lecture/presentation.

M-2 Discussion

M-3 Explanation

Evaluation methods (F - progressive, P - final)S-1 testF

S-2 assessment of student's activity and attitudes towards discussed issues.F

S-3 reportF



study





education

Teachingmethods


methods

Knowledge

C-1 S-1T-A-6T-W-1T-W-2

M-1M-2M-3

WM-WBiHZ_1-_null_W01Student describes methods using in toxicity assessment ofxenobiotics.

T-W-3T-W-4T-W-5

C-1 S-1T-A-1T-A-2

M-1M-2M-3

WM-WBiHZ_1-_null_W02Student desribes the metabolism of toxins and mechanisms oftoxicity

T-A-5

Skills

C-3 S-3T-A-3

M-3WM-WBiHZ_1-_null_U01Student uses the computational methods in toxicity, exposureand risk assessment.

T-A-4


C-1 S-2

T-A-1T-A-2T-A-3T-A-4T-A-5T-A-6

M-1M-3

WM-WBiHZ_1-_null_K01Student understands the need to reduce the use of animals intoxicological studies.




2,03,0 The student lists and discribes the methods uses in toxicity assessment.

3,54,04,55,0


2,03,0 Student describes phases of xenobiotic's metabolism and lists mechanisms of toxicity.

3,54,04,55,0


2,03,0 Student is able to calculate: the LD50 for a specific subtance (with using at least two methods), RfD, LOAL.

3,54,04,55,0


2,03,0 Student explains the reasons for reducing the number of animals in experimental studies.

3,54,04,55,0

Required reading1. Michael Balls, Robert Combes, Andrew Worth, The History of Alternative Test Methods in Toxicology (1st Edition), Elsevier, 2018

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-20-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Medical and Veterinary Microbiology







Other teachers

Prerequisites



C-1 The course aims are to provide a comprehensive theoretical and practical knowledge of medical and veterinarymicrobiology.

Course content divided into various forms of instruction Number of hoursT-L-1 Methods of culturing clinically significant bacteria 2

T-L-2 Conditions of cultures of clinically significant bacteria 2

T-L-3 Microscopic examination of microorganisms 2

T-L-4 Detection and identification of various kind of clinically significant microorganisms 4

T-L-5 Determination of antibiotic susceptibility of pathogenic bacteria 3

T-L-6 Study of biochemical activity of clinically significant microorganisms 2

T-W-1 Information about working in clinical microbiological laboratory 2

T-W-2 Methods for determination and controlling growth of pathogenic bacteria 2

T-W-3 Detection and identification of various kind of clinically significant microorganisms 2

T-W-4 Determination of antibiotic susceptibility of pathogenic bacteria 2

T-W-5 Upper Respiratory Tract Infections 1

T-W-6 Lower Respiratory Tract Infections 1

T-W-7 Gastrointestinal Tract Infections 1

T-W-8 Genitourinary Tract Infections 1

T-W-9 Skin and Soft Tissue Infections 1

T-W-10 Immunoprophylaxis and Immunotherapy 2









Medical and Veterinary Microbiology


M-2 Laboratory






study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_1-_null_W01The student can choose the appropriate research techniques forthe isolation and identification of clinical strains of bacteria andfungi.

SkillsWM-WBiHZ_1-_null_U01The student uses skills on the methods of diagnosis, preventionand treatment of bacterial and fungal diseases.

Other social / personal competencesWM-WBiHZ_1-_null_K01The student demonstrates responsibility and awareness ofdecisions when conducting diagnostic tests and proceduresprevention and treatment of infectious diseases of humansanimals.



2,03,03,54,04,55,0


2,03,03,54,04,55,0


2,03,03,54,04,55,0




[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-22-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Molecular Biology







Other teachers

PrerequisitesW-1 Basics of molecular genetics

Module/course unit objectivesC-1 Get knowledge about molecular gene organization


Course content divided into various forms of instruction Number of hoursT-L-1 Extraction and purification of cellular RNA 3

T-L-2 Gel electrophoresis to check RNA. 2

T-L-3 PCR- clean up and cloning reaction 3

T-L-4 Primer design, CAPS search. 2

T-L-5 Genomic sequence analysis: gene finding, BLAST searching, genome annotation. 3

T-L-6 DNA sequence analysis - cloning strategies, computer-assisted restriction analysis. 2

T-W-1 Introduction: History of molecular biology, DNA as the genetic material, nucleic acid structure,hybridization. 2

T-W-2 DNA replication, bacterial and eucaryotic DNA polymerases. 2

T-W-3 Gene structure, replication, transcription, translation. 4

T-W-4 RNA processing: splicing, spliceosomes, snRNPs, self splicing introns, polyadenylation. 4

T-W-5 Eucaryotic transcriptional regulation, transposons, recombination. 3












Molecular Biology


S-2 PresentationF



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_1-_null_W01Understanding of molecular mechanisms of genome functioning

SkillsWM-WBiHZ_1-_null_U01Ability to differentiate basic processes ongoing in a living cell

Other social / personal competencesWM-WBiHZ_1-_null_K01Teaching and explaining of basic molecular processes ongoingin cells of living organisms



2,03,03,54,04,55,0


2,03,03,54,04,55,0


2,03,03,54,04,55,0

Required reading1. Weaver R., Hill M.G., Miolecular Biology, 2001

2. Watson J.D., Molecular Biology of the gene, Pearson Education, 2013

Supplementary reading1. Albertis B et all., Molecular Biology of the Cell, Garland Publishing, New York, 2009

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-23-Z

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Molecular Diagnostics







Other teachers

PrerequisitesW-1 Basic knowledge of molecular technique.

Module/course unit objectivesC-1 Acquaint the students to versatile tools and techniques employed in diagnostic molecular and recombinant DNA technology.

Course content divided into various forms of instruction Number of hoursT-L-1 Preventing contamination, DNA extraction, asses purity of DNA 3

T-L-2 Application of DNA testing. preparation the samples to analysis. 3

T-L-3 Molecular laboratory diagnostic of different genetic deseases. 7

T-L-4 Analysis of results 2

T-W-1 Nucleid acid structure, extraction and probe preparation. 3

T-W-2 Manipulation DNA sequences with versatile DNA modifying enzymes. 3

T-W-3 DNA amplification methods, mutational analysis, sample preparations. 3

T-W-4 Alternative methods for amplified nucleic acid testing 2

T-W-5 Genes therapy, applications in diagnostic of genetic disorden, human genome project. 4

Student workload - forms of activity Number of hoursParticipation in laboratories 15A-L-1











S-2 PresentationF

Molecular Diagnostics



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_1-_??_W01Studenst knows the diagnostic basics used in the laboratory

SkillsWM-WBiHZ_1-_??_U01Student can indenpendently perform genetic diagnostic test

Other social / personal competencesWM-WBiHZ_1-_??_K01Can explain the purpose of use genetic diagnostic test



2,03,03,54,04,55,0


2,03,03,54,04,55,0


3,03,54,04,55,0

Required reading1. Bruns D.E, Ashwood E.R., Burtis C.A., Fundamentals of molecular diagnostic, 2011

2. Coleman W.B., Molecular Diagnostic, Springer, 2005

Supplementary reading1. Debnath M.,, Molecular diagnostic: Promises and possibillities, Springer, Netherland, 2010

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-24-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Molecular Modeling of Enzymes







Other teachers

PrerequisitesW-1 Knowledge of organic and inorganic chemistry, biochemistry, biophysics, English at intermediate level,

Module/course unit objectivesC-1 Developing skills for selection of appropriate tools to solve and analyze the structure of enzymes

Course content divided into various forms of instruction Number of hoursT-L-1 Analysis of enzymes structural properities by molecular modeling software 6

T-L-2 Prediction of tretairy structure of alpha - amylase form A. niger 4

T-L-3 Modeling of catalytic properities of alpha - amylase from A. niger 5

T-W-1 Methods and source of obtaining information about the structure of enzymes 1

T-W-2 Methods of functional analysis of the primary structure of enzymes 2

T-W-3 Methods of prediction and analyze the secondary structure of enzymes 2

T-W-4 In silico methods to prediction and analyze the tretiary structure of enzymes 6

T-W-5 Methods for prediction and modeling functional properities of enzymes 4








M-2 disscusion



Evaluation methods (F - progressive, P - final)S-1 projektF

S-2 projektP

Molecular Modeling of Enzymes



study





education

Teachingmethods


methods

Knowledge

C-1 S-1M-1M-2M-3M-4

WM-WBiHZ_1-_??_W01Student has knowledge about enzyme molecular structureorganisation principles and methods of its analysis,determination and modification with use a bioinformatics tools.

Skills

C-1 S-1T-W-1T-W-2T-W-3

M-1M-3M-4

WM-WBiHZ_1-_??_U01Student choose and apply correctly a molecular modeling toolsfor enzyme structure analysis and designing

T-W-4T-W-5


C-1 S-2T-L-1T-L-2T-L-3T-W-1

M-1M-2M-3M-4

WM-WBiHZ_1-_??_K01Student know and understand a consequences of modificationsof the enzyme native structure




2,03,0 Preparation of molecular modeling project in base of indicated enzyme primary structure

3,54,04,55,0


2,03,0 Preparation of molecular modeling project in base of indicated enzyme primary structure

3,54,04,55,0


3,0 Preparation on indicated time a project report

3,54,04,55,0

Required reading1. Huzefa Rangwala, George Karypis, Introduction to Protein Structure Prediction: Methods and Algorithms, 2010



4. Arieh Warshel, Computer Modeling of Chemical Reactions in Enzymes and Solutions, Wiley, 1997


[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-25-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Proteomics






Herosimczyk Agnieszka ([email protected])Leading teacherDratwa-Chałupnik Alicja ([email protected]), Herosimczyk Agnieszka([email protected]), Lepczyński Adam ([email protected]),Michałek Katarzyna ([email protected]), Ożgo Małgorzata([email protected])

Other teachers

PrerequisitesW-1 Basic of the cell biology and the protein biochemistry.

Module/course unit objectivesC-1 Theoretical and practical knowledge of gel-based and chromatographic protein separation techniques.

C-2 The ability of the participants to use advanced bioinformatic tools to analyse proteomic data (1-D and 2-D gels, massspectra).

C-3 Practical use of MALDI-TOF MS (matrix-assisted laser desorption/ionisation time of flight mass spectrometer) for proteinidentification.

Course content divided into various forms of instruction Number of hoursT-L-1 Sample preparation techniques for proteomic analysis. 2

T-L-2 Protein separation using two-dimensional electrophoresis (2-DE). 2

T-L-3 Protein separation using SDS-PAGE (1-DE). 2

T-L-4 Protein gel staining methods. 2

T-L-5 Identification of proteins using mass spectrometer MALTI-TOF. 3

T-L-6 Identification of proteins using Western-blot technique. 2

T-L-7 1-DE and 2-DE gel image acquisition and bioinformatic analysis. 2

T-W-1 Introduction to proteomics. Biological significance of post-transcriptional and post-translational proteinmodifications. Proteome organization. The general principles of proteomic analysis. 2

T-W-2Gel-based protein separation techniques. The components of resolving gel matrix. Sodium-dodecylpolyacrylamide gel electrophoresis (SDS-PAGE), the principle and application of native PAGEelectrophoresis. Two dimensional electrophoresis (2-DE) – the principle of the method, samplepreparation for 2-DE, IPG strips, isoelectric focusing.

2

T-W-3Protein detection methods: coomassie stain, silver stain, negative ion staining (copper, zinc),autoradiography, fluorography, fluorescent staining. Two-dimensional difference in gel electrophoresis(2D-DIGE) – the principle and application of the method. Image acquisition and analysis of 1-D and 2-Dgels. 1-D and 2-D gels analysis softwares.

2

T-W-4 Application of mass spectrometry (MS) for protein identification. Ionization methods in massspectrometry. Types of mass analyzers. Peptide mass fingerprinting (PMF). 2

T-W-5Chromatographic methods for protein separation. Liquid chromatography (LC). Two-dimensional liquidchromatography (2-D LC). The proteomic strategies based on liquid chromatography: LC-MS, LC-MS/MS, multidimensional LC-MS/MS. Affinity chromatography (AC).

2

T-W-6 Identification of proteins using Western-blot technique. Sample preparation. Methods of proteintransfer. Incubation with antibodies. Visualisation. 3

T-W-7 Branches of proteomics: structural, functional and clinical. 2

Student workload - forms of activity Number of hours

Proteomics

Student workload - forms of activity Number of hoursParticipation in laboratories. 15A-L-1

Self work with publications. 15A-L-2

Laboratory project preparation. 20A-L-3






Teaching methods / toolsM-1 Theoretical lectures.

M-2 Discussion during laboratory classes.

M-3 Project preparation.

Evaluation methods (F - progressive, P - final)S-1 Project presentation in the writing form.F

S-2 Writing test.P



study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-2


M-1WM-WBiHZ_1-_??_W01Student can enumerate and describe commonly usedtechniques used in the study of proteins.

T-W-5T-W-6T-W-7

Skills

C-1C-3 S-1

T-L-1T-L-2T-L-3T-L-4

M-2M-3

WM-WBiHZ_1-_??_U01Student is able to use commonly known proteomic techniquessuch as: 1-DE, 2-DE, MALDI-TOF MS and Western-blot.

T-L-5T-L-6T-L-7


C-1C-2C-3

S-1S-2

T-L-1T-L-2T-L-3T-L-4T-L-5T-L-6T-L-7

M-1M-2M-3

WM-WBiHZ_1-_??_K01Student is aware that there is a number of methods to analysethe different levels of protein changes in response to variousphysiological/patophysiological stimmuli in the biologicalmaterial.

T-W-1T-W-2T-W-3T-W-4T-W-5T-W-6T-W-7



2,0

3,0- student has a basic knowledge in the frame of the material program- student exerts low attitude to knowledgein the frame of expressing knowledge student makes a lot of mistakes

3,54,04,55,0


2,03,0 Student, with a help of a teacher is able to meet the challenges of preparing an appropriate laboratory protocol.

3,54,04,55,0


3,0 Student shows a moderate interest in participating in a verbal discussion with the teacher and colleagues during the classes.

3,54,04,55,0

Required reading1. Sheehan D., Tyther R. (Ed.)., Two-dimensional electrophoresis protocols., Humana Press, New York, 2009

2. Garfin D., Ahuja S. (Ed.), Handbook of isoelectric focusing and proteomics., Elsevier Academic Press, Amsterdam, 2005

3. Heftmann E. (Ed.)., Chromatography, sixth edition., Elsevier Academic Press, Amsterdam, 2004

Required reading4. Walker J.M. (Ed.), second edition., The proteomics protocols handbook., Humana Press, New Jersey, 2002

5. Rabilloud T. (Ed.), Proteome research: two-dimensional gel electrophoresis and identification methods., Springer, Berlin, 2000

6. Hames B.D. (Ed.), third edition., Gel electrophoresis of proteins: a practical approach., Oxford University Press, England, 1998

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-26-L

4,0

credits french

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Protéomique






Ożgo Małgorzata ([email protected])Leading teacherHerosimczyk Agnieszka ([email protected]), Lepczyński Adam([email protected]), Ożgo Małgorzata ([email protected])Other teachers

PrerequisitesW-1 la connaissance de la biochimie, de la biologie moleculaire, la genetique


C-1La Protéomique a pour objectif la formation d’étudiants capables d’identifier et/ou dedéterminer la structure de molécules biologiques simples ou complexes de toute nature (protéine, acide nucléiques,lipides), soit dans le cadre d’une démarche analytique, soit dans le cadre d’une démarche d’analyse globale dumétabolisme.


T-L-1Electrophorèse en gel de polyacrylamide contenant du dodécylsulfate de sodium (SDS-PAGE), leprincipe et les applications de l'électrophorèse sur gel natif PAGE. Electrophorèse bidimensionnelle (2-DE) – principe de la méthode, préparation des échantillons pour la 2-DE, bandes d'IPG (IPG strips),focalisation isoélectrique.

4

T-L-2

Méthodes de détection des protéines: coloration au Bleu de Coomassie, coloration à l'argent, colorationinverse avec des ions (cuivre, zinc), autoradiographie, fluorographie, coloration fluorescente. Analysedifférentielle sur un gel unique (two-dimensional difference in gel electrophoresis 2D-DIGE) – principeet applications de la méthode. Acquisition d'image et analyse de gels 1D et 2D. Logiciels d'analyse desgels 1D et 2D.

4

T-L-3 Utilisation pratique du MS MALDI-TOF (spectromètre de masse matrix-assisted laserdesorption/ionisation time of flight) pour l'identification de protéines 7

T-W-1Introduction à la protéomique. Importance biologique des modifications post-transcriptionnelles etpost-translationnelles des protéines. Organisation du protéome. Les principes généraux de l'analyseprotéomique. Techniques de séparation des protéines basées sur gel. Les composants de la matrice dugel de séparation.

4

T-W-2

Applications de la spectrométrie de masse (MS) pour l'identification des protéines. Méthodesd'ionisation en spectrométrie de masse. Types d'analyseurs de masse. Cartographie peptidiquemassique (peptide mass fingerprinting PMF). Méthodes chromatographiques pour la séparation desprotéines. Chromatographie en phase liquide (LC). Chromatographie liquide bidimensionnelle (2D LC).Les stratégies protéomiques basées sur la chromatographie liquide : LC-MS, LC-MS/MS, LC-MS/MSmultidimensionnelle. Chromatographie d'affinité. Types de protéomique : structurelle, fonctionnelle etclinique.

7

T-W-3 Techniques de séparation des protéines basées sur gel. 4

Student workload - forms of activity Number of hoursparticipation en classe 15A-L-1

préparation pour les classes 10A-L-2

consultations 10A-L-3

étude de la litérrature recommandée 15A-L-4

préparation du protocol 10A-L-5

participation en classe 15A-W-1

Prot?omique

Student workload - forms of activity Number of hoursétude de la litérrature recommandée 23A-W-2

consultations 20A-W-3

test écrit 2A-W-4

Teaching methods / toolsM-1 présntation oral

M-2 travaux pratiques

Evaluation methods (F - progressive, P - final)S-1 test écritF

S-2 preparation raportF



study





education

Teachingmethods


methods

Knowledge

C-1 S-1T-W-1T-W-2 M-1

WM-WBiHZ_1-_null_W01l'élève peut énumérer et décrire les techniques courammentutilisées dans l'étude des protéines

T-W-3

Skills

C-1 S-2T-L-1T-L-2 M-2

WM-WBiHZ_1-_null_U01l'étudiant est capable d'utiliser des techniques protéomiquescommunément connues comme: MALDI TOF, Western Bloting,2DE

T-L-3

C-1 S-2T-L-1T-L-2 M-1

M-2

WM-WBiHZ_1-_null_U02l'étudiant est capable d'utiliser des techniques protéomiquescommunément connues comme: MALDI-TOF, 2DE, WesternBloting

T-L-3


C-1 S-2

T-L-1T-L-2T-L-3 M-1

WM-WBiHZ_1-_null_K01L'étudiant est conscient qu'il existe un certain nombre deméthodes pour analyser les différents niveaux de protéines enréponse à diverses stimulations physiologiques dans le matérielbiologique.

T-W-1T-W-2T-W-3

C-1 S-2

T-L-1T-L-2T-L-3 M-2

WM-WBiHZ_1-_null_K02L'étudiant est conscient qu'il existe un certain nombre deméthodes pour analyser les différents niveaux de protéines enréponse à diverses stimulations physiologiques dans le matérielbiologique

T-W-1T-W-2T-W-3



2,0

3,0l'étudiant a une connaissance de base dans le cadre du programme matérielles étudiants ont une faible attitude vis-à-vis des connaissancesdans le cadre de l'expression des connaissances, l'étudiant fait beaucoup d'erreurs

3,54,04,55,0


2,03,03,54,04,55,0

WM-WBiHZ_1-_null_U02

2,03,03,54,04,55,0


2,03,03,54,04,55,0


2,03,03,54,04,55,0

Required reading1. Sheehan D., Tyther R. (Ed.)., Two-dimensional electrophoresis protocols, Humana Press, New York, 2009

2. Garfin D., Ahuja S. (Ed.)., Handbook of isoelectric focusing and proteomics., Elsevier Academic Press, Amsterdam, 2005

3. Walker J.M., The proteomics protocols handbook, Humana Press,, New Jersey, 2002

Supplementary reading1. Rabilloud T., Proteome research: two-dimensional gel electrophoresis and identification methods, Springer, Berlin, 2002

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-27-L

4,0

credits english

ECTS (forms) 4,0

Level first cycle

Area(s) of study


Module

Course unit Vaccinology







Other teachers

PrerequisitesW-1 Basic lab knowledge and skills.

W-2 Ability to pipet, make solutions and dilutions and to execute protocols which require the use of sterile techniques.

W-3 Basic knowledge of microbiology and immunology.


C-1 The course aims are to provide a comprehensive theoretical and practical knowledge of vaccinology, including theproduction of the vaccines.

Course content divided into various forms of instruction Number of hoursT-L-1 Preparation of vaccine 10

T-L-2 Evaluation of prepared vaccine 5

T-W-1 Immunological aspects of vaccines 3

T-W-2 Composition and types of vaccines 3

T-W-3 Vaccination of humans and animals 3

T-W-4 Methods for the preparation of vaccines 3

T-W-5 Vaccines for tomorrow 3










M-2 Laboratory

Evaluation methods (F - progressive, P - final)S-1 Writing testF



Vaccinology



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_1-_null_W01The student knows the immunological basics related to theproduction bioproducts, knows the role of adjuvants and carriersfor synthetic vaccines, knows the rules of prevention andtreatment of certain human and animal diseases using vaccinesand immunomodulators or autovaccines.

SkillsWM-WBiHZ_1-_null_U01Student is able to classify the vaccine and analyze the reactionsof the immune system after immunization.




2,03,03,54,04,55,0


2,03,03,54,04,55,0


Required reading1. L. M. Prescott, Microbiology, McGraw-Hill Science, 2002

2. C. L. Gyles, J. F. Prescott, J. G. Songer, C. O. Thoen, Pathogenesis of Bacterial Infections in Animals 4th Ed, Blackwell Publishing, 2010

3. Roitt I., Brostoff J., Male D., Immunology, Brema, 1998

SECOND DEGREE (MASTER)

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-01-Z

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Animal Embryology






Stankiewicz Tomasz ([email protected])Leading teacherBłaszczyk Barbara ([email protected]), Stankiewicz Tomasz([email protected]), Udała Jan ([email protected])Other teachers

PrerequisitesW-1 The knowledge of animal physiology and biotechnology in animal reproduction.

Module/course unit objectivesC-1 To acquaint students with the course of the various stages of embryonic and fetal development.

C-2 To acquaint students with the mechanisms that control the development of embryonic and fetal development withparticular emphasis on the role of amniotic fluid and placenta.


T-L-1 The types of the placentas in various species of mammals and anatomical differences in theirconstruction. 3

T-L-2 The structure and function of the fetal membranes. 3

T-L-3 The pregnancy (calculation date of birth). The parturition. The development of the fetus and fetalmaturity. 3

T-L-4 The development of the hematopoietic system: the development of erythroid and white blood cells. 3

T-L-5 Evaluation age of the embryo and fetus based on the size and shape of the body. 3

T-W-1 Embryology as a scientific discipline and a range of modern embryology of animals. 2

T-W-2 The course and the types of implantation. 2

T-W-3 The role of fetal-placental endocrine system in the fetal development. Hormonal regulation ofpregnancy and parturition. 2

T-W-4 The development and metabolism of the embryo in the initial period of postimplantation. Themechanism of the formation of the twin pregnancy. 2

T-W-5 Adapting to embryonic and fetal life and the role of the transitional organs. 2

T-W-6 The mechanisms of organogenesis and chronological division of the differentiation of the final organs. 2

T-W-7 The differentiation of mesodermal organs (somites, median mesoderm). 2

T-W-8 The embryonic induction. The possibilities of the using cord blood in the transplantation. 1

Student workload - forms of activity Number of hoursThe participation in the classes. 15A-L-1


Preparing to pass the exercise. 35A-L-3





Animal Embryology

Teaching methods / toolsM-1 The informative lecture with the use of multimedia techniques.

M-2 Activating methods (preparation and presentation of papers by students, discussion).

M-3 The demonstration, laboratory exercises (the macro- and microscopic observation).

Evaluation methods (F - progressive, P - final)S-1 The rating presentations prepared and delivered by students (teamwork) and engage in the discussion.F

S-2 The current control of the proper operation of students in laboratory classes.F

S-3 The rfinal test covering a range of content of lectures and exercises.P



study





education

Teachingmethods


methods

Knowledge

C-1S-1S-2S-3


M-1M-2M-3

WM-WBiHZ_2-_null_W01Student defines the basic terminology in the field ofembryology. He describes the various stages and mechanismsof embryonic and fetal development.


C-2 S-2S-3

T-L-1T-L-2 M-1

M-3

WM-WBiHZ_2-_null_W02The student knows the course of implantation. It describes thefunction of the auxiliary organs and shows the importance offetal-placental endocrine system in fetal development. Heknows the mechanism of parturition.

T-W-5

Skills

C-1 S-2S-3

T-L-1T-L-2T-L-3 M-1

M-3

WM-WBiHZ_2-_null_U01Student is able to determine the degree of development of theembryo and fetus on the basis of the morphologicalcharacteristics. He is able to assess the species adherence ofplacentas and fetal membranes. He points to the distinctivenessin the morphological images of the fetal blood.

T-L-4T-L-5


C-1C-2 S-3

T-L-1T-L-2T-L-3T-L-4T-L-5

M-1M-3

WM-WBiHZ_2-_null_K01After completing the course, the student will have a basis forstudying disciplines related to the obtaining of extracorporealembryos in vitro, the cloning, transgenesis and transplantation.


C-1C-2

S-1S-2


M-2M-3

WM-WBiHZ_2-_null_K02The student analyzes the problem of taking a group discussion.




2,03,0 The student knows the basic terminology in the field of animal embryology. He mentions the successive stages of embryonic

development and mechanisms, some of which he describes.3,54,04,55,0


2,0

3,0The student knows the types of implantation. He mentions successive stages of implantation. He lists and knows the functionof the auxiliary organs. He knows the importance of the mechanism of parturition and fetal-placental endocrine system inthe developing fetus.

3,54,04,55,0


2,03,0 On the basis of criteria a student assesses the degree of development of the embryo and fetus. He recognizes some types of

placentas. He points to the distinctiveness in the morphological picture of blood in some species.3,54,04,55,0


2,03,0 The student is aware of existing knowledge in further study of scientific disciplines related to gamete manipulation.

3,54,04,55,0


2,03,0 The student attempts to analyze the problem and take part in the discussion.

3,54,04,55,0

Required reading1. T. A. McGeady, P. J. Quinn, E. S. FitzPatrick, M. T. Ryan, Veterinary Embryology, Blackwell Publishing, 2006

Supplementary reading1. Poul Hyttel, Fred Sinowatz, Morten Vejlsted, Morten Vejlsted, Essentials of Domestic Animal Embryology, Saunders Ltd., 2010

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-02-Z

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Animal Obsterics






Stankiewicz Tomasz ([email protected])Leading teacher

Other teachers

PrerequisitesW-1 The knowledge of animal reproduction and embryology.


C-1 The aim of this course is to acquaint of students with the parturition and the principles of obstetric care of the mother andnewborn.

C-2 The students will be acquainted with the rules to interpret signs of the impending parturition and properly reacting in caseof any disturbance in its course.

C-3 The students will be also acquainted with the assess of health risks for the mother and newborn baby during the perinatalperiod.


T-A-1 Severe parturition - clinical procedure (rapid intervention, interview, examination). General data.Calculating the date of birth. 3

T-A-2 Obstetric examination. Analytical studies in the evaluation of the pregnancy and perinatal period. 3

T-A-3 Postpartum care of the mother and newborn. Determining the age and maturity of the fetus based onmorphometric measurements. 3

T-A-4 Severe parturition in cows, mares, sheep, goats and sows. The analysis of different cases. 3

T-A-5 Severe parturition in dogs and cats. The analysis of different cases. 3

T-W-1 The parturition in cows and mares. Symptoms and stages of the parturition. Interference in thephysiological parturition. 3

T-W-2 The parturition in sheep, goats and sows. Symptomatic stages of childbirth. Interference in thephysiological delivery. 3

T-W-3 The parturition in bitches and cats. Symptoms and stages of the parturition. Interference in thephysiological parturition. 3

T-W-4 Pathology of the pregnancy. Multiple fertilization. Additional fertilization. Ectopic pregnancy. 3

T-W-5 Rupture of the vagina and uterus. Hernia of pregnant uterus (types of hernias). Colpoptosia. Toxemiaof the pregnancy. Prolonged pregnancy. 3

Student workload - forms of activity Number of hoursThe participation in the classes. 15A-A-1

Participation in the consultations. 10A-A-2

Preparing to pass the exercise. 35A-A-3




Teaching methods / toolsM-1 The informative lecture with the use multimedia techniques.

Animal Obsterics

Teaching methods / toolsM-2 Activating methods (preparation of presentations by students).

Evaluation methods (F - progressive, P - final)S-1 The evaluation of presentations prepared by students (teamwork).F

S-2 The final test covering the range of content lectures and auditoriaP



study





education

Teachingmethods


methods

Knowledge

C-1 S-1T-W-1T-W-2T-W-3

M-1M-2

WM-WBiHZ_2-_null_W01Student knows of the course of parturition and the principles ofobstetric care of the mother and newborn.

T-W-4T-W-5

Skills

C-2C-3

S-1S-2

T-A-1T-A-2T-A-3 M-1

M-2

WM-WBiHZ_2-_null_U01Student is able to interpret signs of the impending parturitionand properly react in case of any disturbance in its course.Student is able assess the health risks for the mother andnewborn baby during the perinatal period.

T-A-4T-A-5


C-1C-2C-3

S-1

T-A-1T-A-2T-A-3T-A-4T-A-5

M-1M-2

WM-WBiHZ_2-_null_K01After completing the course, the student will have a basis forstudying disciplines related to obstetrics. The student analyzesthe problem of taking a group discussion.




2,03,0 Student knows only some facts of the course of parturition and the principles of obstetric care of the mother and newborn.

3,54,04,55,0


2,0

3,0Student is able to interpret only some signs of the impending parturition and does not properly react in case of anydisturbance in its course. Student is able assess the health risks for the mother and newborn baby during the perinatalperiod only with the help of the teacher.

3,54,04,55,0


2,03,0 The student analyzes the problem of taking a group discussion only in selected topics.

3,54,04,55,0

Required reading1. Peter GG Jackson:, Handbook of Veterinary Obstetrics., Second editon, Elsevier, 2004

Supplementary reading1. David E. Noakes, Geoffrey H. Arthur, Timothy J. Parkinson, Gary C. W. England, Arthur’s Veterinary Reproduction and Obstetrics., W.B.Saunders, Eight edition, 2001

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-04-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Basic Microbiology







Other teachers

Prerequisites


Module/course unit objectivesC-1 The course aims are to provide a comprehensive theoretical and practical knowledge of animal microbiology.

Course content divided into various forms of instruction Number of hoursT-L-1 Information about working in microbiological laboratory 1

T-L-2 Bacterial structure, growth, cultivation 2

T-L-3 Methods of culturing bacteria isolated from different animals 2

T-L-4 Conditions of culturing microorganisms isolated from animals 2

T-L-5 Basics of mycological examination 2

T-L-6 Detection and identification of various kind of microorganisms isolated from animals 2

T-L-7 Identification of virulence factors produced by microorganisms isolated from animals 2

T-L-8 Molecular diagnostics of microorganisms isolated from animals 2

T-W-1 General bacteriological examination of material collected from animals 2

T-W-2 Detection and identification of various kind of microorganisms isolated from animals 2

T-W-3 Zootechnical microbiology 2

T-W-4 Zoonotic diseases 2

T-W-5 Microbiology of zootechnical products 2

T-W-6 Antimicrobial chemotherapy 2

T-W-7 Health and safety in microbiological laboratory 1

T-W-8 Antibiotic susceptibility of animal pathogens 2









Basic Microbiology


M-2 Laboratory






study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_2-_null_W01The student can choose the appropriate techniques forexamination and identification of bacteria and fungi isolatedfrom animals

SkillsWM-WBiHZ_2-_null_U01The student uses skills on diagnostics of bacteria and fungi.

Other social / personal competencesWM-WBiHZ_2-_null_K01The student demonstrates responsibility and awareness of thedecisions made during the conduct of microbiological tests.



2,03,03,54,04,55,0


2,03,03,54,04,55,0


2,03,03,54,04,55,0




[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-06-L

4,0

credits german

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Biotechnik der Fortpflanzung beiLandwirtschaftlichen Nutztieren







Lasota Bogdan ([email protected])Leading teacher

Other teachers

PrerequisitesW-1 Grundlagen der Tierzucht und Physiologie


C-1Kennenlernen von Biotechniken, die bei den Nutztieren angewandt werden. Aneignung von Kenntnissen und Fertigkeitenbei Durchführung von bestimmten Biotechniken. Erkennung von technischen, ethischen und gesellschaftlichen Problemenbei der Anwendung von biotechnischen Massnahmen bei Nutztieren

Course content divided into various forms of instruction Number of hoursT-L-1 Geräte und Materialien für Embryonengewinnung 2

T-L-2 Eizellgewinnung mit verschiedenen Methoden. 2

T-L-3 Spülung von Embryonen aus Eileiter und Uterus 4

T-L-4 Einfrieren von Embryonen mit der Vitrifikationsmethode 2

T-S-1 Künstliche Besamung bei ausgewählten Tierarten. Anwendungsbeispiele für biotechnischen Methodenzur Steuerung des Brunstzyklus und Geburtssynchronisation bei Nutztieren. 2

T-S-2 Induktion der Superovulation bei weiblichen Nutz- und Labortieren. 2

T-S-3 Handhabung von Embryonen nach ihrer Gewinnung. Klassifizierung von Embryonen aufgrund ihrermorphologischen Beurteilung 3

T-S-4 Ethische und gesellschaftiche Aspekten des Klonens 2

T-S-5 Repetitorium, wahlfreie Themen und Problemen 1

T-W-1 Angewandte Biotechniken bei Nutztieren (künstliche Besamung, Brunstzyklussteuerung) 2

T-W-2 Biotechniken in der Reproduktion der Nutztiere (Embryotransfer, Klonen) 2

T-W-3 Grundlagen der Kryobiologie 1

T-W-4 Physikalische und chemische Prozesse in den biologischen Objekten bei der Kühlung 1

T-W-5 Methoden der Kryokonservierung von Eizellen und Embryonen. Die Anwendung der Kryokonservierungvon Eizellen und Embryonen bei Nutz- und Labortieren 2

T-W-6 Klonen von Säugetieren 2

Student workload - forms of activity Number of hoursTeilnahme an Übungen 10A-L-1

Theoretiische Vorbereitung zur Übungen 10A-L-2

Auswertung und Besprechung der Übungen 10A-L-3

Teilnahme an Seminaren 10A-S-1

Vorbereitung zur Seminaren 10A-S-2

Biotechnik der Fortpflanzung bei Landwirtschaftlichen Nutztieren

Student workload - forms of activity Number of hoursVorbereitung zur Disskusion über ethische und gesellschaftliche Fragen der Anwendung vonfortschrittenen Biotechniken bei Tieren 10A-S-3

Teilnahme an Vorlesungen 10A-W-1

Vorbereitung zur Diskusson 25A-W-2

Vorbereitung der Präsentation 25A-W-3


M-1 InformationsvorlesungDiskussion, praktische Übung,

Evaluation methods (F - progressive, P - final)S-1 Beurteilung von laufenden Ergebnissen- Aktivität, VorbereitungF

S-2 Beurteilung der Präsesntaion zum angegeben ThemaP



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_2-_??_W01Der Student kennt die Biotechniken, die bei der Fortpflanzungvon Tieren verwendet werden, kann den Verlauf vonFortpflanzungsprozessen und die Wirkung ausgewählterbiologisch aktiver Verbindungen und Zubereitungen erklären,die zu Regulierung dieder Prozesse verwendet werden können.

SkillsWM-WBiHZ_2-_??_U01Der Student kann die richtige Biotechnik für bestimmte Tierartauswählen. Kann die Grundausstattung für ausgewählteBiotechniken aufzeigen und vorbereiten.

Other social / personal competencesWM-WBiHZ_2-_??_K01Der Student kann die Vor- und Nachteile des Einsatzes derTechnik zur Kontrolle der Fortpflanzungsprozesse bei Tierenaufzeigen, erlangt die Fähigkeit, die Auswirkungen seinerNutzung selbstständig zu interpretieren, kann bei sozialenKontakten eine aktive Position in technischen und ethischenFragen im Zusammenhang mit der Verwendung von Biotechnikeinnehmen



2,03,03,54,04,55,0


2,03,03,54,04,55,0


3,03,54,04,55,0

Required reading1. Niemann H., Ehling Ch., Falge R.,, Biotechnological methods for preservation and maintaince of farm animals genetic resources,,Schriftenreihe des BML “Angewandte Wissenschaft”, 1997, 465, 65-76.

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-07-Z

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Biotechnology and Genetic Engineering







Other teachers

PrerequisitesW-1 Basics of biotechnology methods

Module/course unit objectivesC-1 Get knowledge about biotechnology and genetic engineering


Course content divided into various forms of instruction Number of hoursT-L-1 Isolation of genomic DNA came from different tissue 4

T-L-2 Enzymes in genetic engineering 2

T-L-3 Methods of nucleic acid detection. 2

T-L-4 Variations in PCR and their applications. 2

T-L-5 Molecular diagnostic in medicin. Analysis of polymorphisms in different gene in human. 5

T-W-1 Role of genes within cells, gene code and elements that control gene expression 4

T-W-2 Marker-assisted sellection for animal breedeng 3

T-W-3 PCR and its applications 2

T-W-4 Introductions and methods in gene therapy 2

T-W-5 Quantification and storage of nucleic acid 2

T-W-6 Construction of genomic library 2












Biotechnology and Genetic Engineering


S-2 PresentationF



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_2-_null_W01Student get knowledge and can define genetic engineeringprocesses in biotechnology

SkillsWM-WBiHZ_2-_null_U01Students can use biotechnology methods relation with nuclearacid analyses

Other social / personal competencesWM-WBiHZ_2-_null_K01Explaining of basic of genetic engeneering



2,03,0 Studenst knows the basic information about genetic engineering using in biotechnology

3,54,04,55,0


2,03,0 Students know how to use the basic analysis of genetic endineering

3,54,04,55,0


2,03,0 Students can explain the basic information abou nuclear acid

3,54,04,55,0




[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-1-08-Z

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Cell Biology






Lepczyński Adam ([email protected])Leading teacherDratwa-Chałupnik Alicja ([email protected]), Herosimczyk Agnieszka([email protected]), Michałek Katarzyna ([email protected]),Ożgo Małgorzata ([email protected])

Other teachers

PrerequisitesW-1 Basicsof biochemistry and physiology

Module/course unit objectivesC-1 To obtain knowledge concerning structure and differentiation of distinct cells.

C-2 To gain insight into the specific functions displayed by cell membrane and various cellular organelles.

C-3 To develop the ability to think critically about issues in cell biology

Course content divided into various forms of instruction Number of hoursT-L-1 Types of cells and tissues. The interdependence between the cell structure and its function 4

T-L-2 Analysis of a cytoskeleton and cell cortex functions on the example of erythrocyte and sperm cells.Experimental destruction of the cell membrane of erythrocytes. 4

T-L-3 Localization, function and signal transduction of taste receptors. 3

T-L-4 Practical recognition of different stages of the processes of mitosis and meiosis 3

T-L-5 Visualization of leucocyte nucleus. 3

T-L-6 The influence of pH and temperature on enzymes activity. 3

T-W-1 Structure of cell membrane. Transport of small molecules across the cell membrane. 2

T-W-2 Principle of cell signaling. Major classes of cell-surface receptor proteins. 2

T-W-3 Structure and function of the cytoskeleton 2

T-W-4 Cell cycle and its regulation. 2

T-W-5 The compartmentalization of cells: rough and smooth endoplasmic reticulum, Golgi apparatus,mitochondrion, lysosome. Mechanism of vesicular transport. 2

Student workload - forms of activity Number of hoursParcitipation in laboratory classes 20A-L-1

Preparation to the laboratory classes 15A-L-2

Studying of recomended literature 15A-L-3







Cell Biology


M-2 laboratory





study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-1

T-W-1T-W-2T-W-3

M-1M-2

WM-WBiHZ_2-_null_W01Student should exhibit a general knowledge of the basicstructures and cell biology-related mechanisms in an eukaryotecell.

T-W-4T-W-5

Skills

C-1C-2C-3

S-2T-L-1T-L-2T-L-3 M-2

WM-WBiHZ_2-_null_U01- describe and carry out basic methods in cell biology- explain the theory behind the practical parts in the course andbe able to summarise and interpret experimental results

T-L-4T-L-5T-L-6


C-3 S-2

T-L-1T-L-2T-L-3T-L-4T-L-5T-L-6

M-2WM-WBiHZ_2-_null_K01Student creates an active attitude, has the ability to holisitcview on the facts in the field of the molecular biology




2,0

3,0- student has a basic knowledge in the frame of the material program- student exerts low attitude to knowledgein the frame of expressing knowledge student makes numerous mistakes

3,54,04,55,0


2,03,0 Student is able to perform basic analyses in the field of cell biology and with the help of the teacher comilate and interpret

their results3,54,04,55,0


2,03,0 Student is aware of biological processes complexity and recognizes the role of molecular mechanisms in maintaining the

cellular homeostasis3,54,04,55,0

Required reading1. Alberts B., Johnson A., Lewis J., Morgan D., Raff M., Roberts K., Walter P., Molecular biology of the cell, Garland Science, Taylor &Francis Group, 2015, 6th edition

Supplementary reading1. Lodish H., Berk A., Zipursky S.L., Matsudaira P., Baltimore D, Darnell J, Molecular Cell Biology, New York: W. H. Freeman, New York,2016, 8th edition2. Karp G., Cell and molecular biology. Concepts and experiments., WileyPlus, 2013, 7th edition

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-09-Z

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Cellular Engineering in Animal Reproduction






Stankiewicz Tomasz ([email protected])Leading teacherBłaszczyk Barbara ([email protected]), Gączarzewicz Dariusz([email protected]), Lasota Bogdan ([email protected]), SeremakBeata ([email protected]), Stankiewicz Tomasz ([email protected]),Udała Jan ([email protected])

Other teachers

PrerequisitesW-1 Knowledge of the basics of biotechnology and biotechnology in animal reproduction.


C-1 Acquainting students with the methods of cellular engineering used in manipulations on gametes and embryos of mammals.

C-2 Acquainting students with the procedure for the transfer of embryos in different species of animals.

C-3 Acquainting students with the possibilities of using cell culture in the studies of mammalian reproductive processes.

C-4 Formation of proper attitude of the students in relation to the use of cell engineering in mammalian reproduction.

Course content divided into various forms of instruction Number of hoursT-L-1 Morphological evaluation of the oocytes by using histological preparations. 3

T-L-2 Obtaining of the oocytes from the ovaries of selected mammals, evaluation of the quality andusefulness of the oocytes for in vitro studies. 3

T-L-3 The preparation of the oocytes for in vitro maturation. The assessment of the degree of maturity of theoocytes in IVM procedure. 3

T-L-4 The evaluation of the sperm. Methods of sperm capacitation and their preparation for in vitrofertilization . 2

T-L-5In vitro fertilization and culture of embryos to the blastocyst stage. The evaluation of the quality ofembryos. The analysis of the physiological state of the female reproductive system as a potentialrecipient of embryos.

4

T-W-1 The history, development and current state in the use of cellular engineering of mammalianreproduction. 2

T-W-2 The reproductive potential of the female. Methods of collection and storage the female gametes. 2

T-W-3 The reproductive potential of the male. Methods of obtaining male gametes. Possibility of using spermin the transgenesis as a carrier foreign of genetic information. 2

T-W-4 In vivo fertilization and in vitro fertilization. Methods for the possibility of using in vitro fertilization invarious mammalian species (insemination of oocytes, intracytoplasmic injection). 4

T-W-5 The possibility of long-term preservation of embryos and the processes that occur during freezing andthawing. The properties and the possibility of germ cell transplantation. 3

T-W-6 The control of gender in livestock - of applications of practical importance. 2

Student workload - forms of activity Number of hoursParticipation in the classes. 20A-L-1

Preparing for laboratory classes. 15A-L-2

Preparing to pass the exercise. 18A-L-3


Cellular Engineering in Animal Reproduction

Student workload - forms of activity Number of hoursParticipation in the classes. 15A-W-1


Preparing to pass the exercise. 35A-W-3

Teaching methods / toolsM-1 The informative lecture using multimedia techniques.

M-2 The demonstration, laboratory exercises (slides, macro- and microscopic observation).

Evaluation methods (F - progressive, P - final)S-1 Current control on the proper operation of laboratory classes by students.F

S-2 Final test covering a range of content lectures.P

S-3 Final test covering a range of exercise program content.P



study





education

Teachingmethods


methods

Knowledge

C-1C-4

S-1S-2S-3


M-1M-2

WM-WBiHZ_2-_null_W01Student knows the most important facts and achievements inthe field of cellular engineering in mammalian reproduction. Heknows the factors that determine the reproductive potential ofmammals. Student specifies and describes methods of cellularengineering used in manipulations on gametes and embryos.


C-2C-4

S-2S-3

T-W-2T-W-4 M-1

WM-WBiHZ_2-_null_W02Student knows methods of embryos collection and transfer. Hedescribes potential benefits of embryo transfer in animalhusbandry.

T-W-5T-W-6

Skills

C-1C-3 S-1

T-L-1T-L-2T-L-3 M-2

WM-WBiHZ_2-_null_U01Students know how to acquire and assess the quality ofgametes. He can carried out sperm capacitation and set up thecultures in procedures IVM, IVF and IVC.

T-L-4T-L-5

C-2 S-2S-3

T-L-5 M-1M-2

WM-WBiHZ_2-_null_U02Student knows how to evaluate the quality of embryos. He cancorrectly schedule of embryo transfer procedure.


C-4 S-1


M-1

WM-WBiHZ_2-_null_K01Student is aware of the importance of the knowledge. He knowsthe advantages and limitations associated with the use ofcellular engineering in mammalian reproduction. The completionof the course will be helpful in his future professional work.




2,0

3,0The student knows some facts and achievements in the field of cellular engineering application in mammalian reproduction.He lists the factors determining the reproductive potential of females and males and lists some of the methods used in themanipulation of gametes and embryos.

3,54,04,55,0


2,03,0 Student lists of embryo collection methods and determines the benefits of embryo transfer.

3,54,04,55,0


2,0

3,0Student is able to obtain of oocytes using a single method. He is able to assess the quality of semen, carry out the spermcapacitation at least one species of animals. He is able to assess the degree of oocytes maturity and assumes cultures forIVM, IVF and IVC.

3,54,04,55,0


2,03,0 Student is able to assess quality of the embryos at least in some stages of development. Student describes a general

scheme embryo transfer procedures but does not include physiological conditions of the female, and species differences.3,54,04,55,0


2,03,0 Student knows only the positives or only the limitations associated with the use of cellular engineering in mammalian

reproduction. Do not take the discussion in this regard.3,54,04,55,0

Required reading1. Hafez E.S.E., Hafez B., Reproduction in farm animals, Lippincott Williams & Wilkins, Philadelphia (U.A), 2000

Supplementary reading1. Gordon I. R., Reproductive technologies in farm animals, CABI Pub, Wallingford, Oxfordshire, Cambridge, MA, 2004

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-10-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Environmental Toxicology








PrerequisitesW-1 Knowledge of issues related to ecology and environmental protection.

Module/course unit objectivesC-1 To acquaint students with the toxicological characteristic of selected environmental pollutants.

C-2 To acquaint students with metabolism of toxins.

C-3 To acquaint students with factors influencing toxicity of xenobiotics.

C-4 To acquaint students with the basic mechanisms of functional disorders and morphological changes in selected organs andsystems.

Course content divided into various forms of instruction Number of hoursT-A-1 Toxicity testing of xenobiotics. Degrees of toxicity. Dose-response relationship. 4

T-A-2 Bioconcetration, bioaccumulation and biomagnification. Determination of BCF, BSAF and BMF (fordifferent types of ecosystems). 2

T-A-3 Toxicological characteristics of metals (Cd, Hg, Pb) and metalloids. Source of pollution, route ofabsorption, fate and mechanism of toxicity. MRLs. 2

T-A-4 Estimation of dietary daily intake of toxic substances. 2

T-A-5 Persistent organic pollutants (POPs) - toxicological characteristcs. 2

T-A-6 Estimation of dietary daily intake of selected POPs. 3

T-W-1Pollution and their fate in aquatic and terrestrial ecosystems.Classes of contaminants. Global transport of pollution. Factors determining the distribution ofpollutants in the environment. Models of pollutants spread in the environment.

2

T-W-2 Metabolism of xenobiotics. 2

T-W-3 Factors affecting the toxicity of xenobiotics (the physicochemical properties - dissociation, solubility,particle size, biological factors - age, sex, individual development). 2

T-W-4The biochemical effects of impurities (induction of detoxifying enzymes, and proteins capable ofbinding to heavy metal inhibition of cholinesterase, endocrine dysfunction, DNA adduct formation).Physiological effects of pollution (osmoregulation disorders, metabolic and neurological). The effects oftoxicological interactions (additive effects, toxicity potentiation, antagonism).

2

T-W-5Mutagenic and carcinogenic effects of xenobiotics. The impact of environmental pollution on thedevelopment of cancer. Types of carcinogens (genotoxic - working directly influence the metabolicactivation; epigenetic - promoters, cytotoxic compounds, modifiers of hormones, immunosuppressivecompounds).

2

T-W-6 Poisons of animal origin (poisons of insects, snakes, scorpions, fish). Symptoms and mechanism oftoxicity. 2

T-W-7 Toxicological characteristics of plastics. Toxicological classification of some preparations used inhouseholds. 3


Environmental Toxicology

Student workload - forms of activity Number of hourspreparation for test 20A-A-2





Teaching methods / toolsM-1 Delivery method, lecture/presentation

M-2 Discussion

M-3 Explanation

Evaluation methods (F - progressive, P - final)S-1 testP

S-2 continuous assessmentF



study





education

Teachingmethods


methods

Knowledge

C-2 S-1T-W-2 M-1

M-3WM-WBiHZ_2-_null_W01The student discusses the toxins biotransformation and factorsaffecting the toxicity of xenobiotics.

T-W-3

C-1C-4 S-1

T-W-4T-W-5 M-1

M-2M-3

WM-WBiHZ_2-_null_W02Student discusses the mechanisms of functional disorders andchanges morphological organs and systems under of selectedtoxins.

T-W-6

C-1 S-1T-A-2T-A-3T-A-4T-A-5

M-1M-2M-3

WM-WBiHZ_2-_null_W03Student characterizes of selected xenobiotics.

T-A-6T-W-1T-W-7

Skills

C-1 S-2T-A-1

M-3WM-WBiHZ_2-_null_U01Student is able to calculate the LD50 for a specific subtancewith using different methods.


C-1C-2C-3

S-2

T-A-1T-A-2T-A-3T-A-4T-A-5T-A-6T-W-1

M-2WM-WBiHZ_2-_null_K01The student demonstrates an active engagement with solvingthe identified problems.




2,03,0 The student describes the fate of toxins in the organism and explains the variability of the toxicity of specified substances.

3,54,04,55,0


2,03,0 Student describes the mechanisms of functional disorders and morphological changes in the organs and systems by the

action of selected toxins.3,54,04,55,0


2,03,0 Student correctly characterizes most of indicated xenobiotics.

3,54,04,55,0


2,03,0 Student is able to calculate the LD50 for a indicated xenobiotic, using as at least 2 methods.

3,54,04,55,0


2,03,0 The student demonstrates an engagement with solving the identified problems but does not engage spontaneously.

3,54,04,55,0

Required reading1. (Eds), General, Applied and Systems Toxicology, John Wiley and Sons, Online ISBN: 9780470744307, 2009, DOI:10.1002/9780470744307

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-11-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Food and nutrition in relation to human health


Administering faculty Katedra Hodowli Trzody Chlewnej, Żywienia Zwierząt iŻywności




Pietruszka Arkadiusz ([email protected])Leading teacher

Other teachers

PrerequisitesW-1 Knowledge on the subject in human physiology and biotechnology


C-1 Knowledge by a student chemical structure and properties of food components, their role in human nutrition, and changesduring technological processes

Course content divided into various forms of instruction Number of hoursT-S-1 Methods of determination of the basic nutrients in feed– introduction 2

T-S-2 Determination of dry matter, ash and crude protein 2

T-S-3 Determination of crude fiber, fiber fractions (NDF, ADL, ADF) and crude fat 3

T-S-4 Assessment of the nutritional protein value 3

T-S-5 Estimate chemical assessment of the nutritional protein value 3

T-S-6 Interpretation of the obtained results and conclusions 2

T-W-1 Human nutrition – basic terms 3

T-W-2 Lipids - role of fatty acids in human health 4

T-W-3 Carbohydrates and glicemic index. 3

T-W-4 Food Additives 4

T-W-5 Conclusions 1

Student workload - forms of activity Number of hoursParticipation in classes 15A-S-1

Reading specific literature 15A-S-2

Preparing for the exam 15A-S-3

Preparing projects in groups 15A-S-4


Reading specific literature 15A-W-2

Preparing for the exam 15A-W-3

Preparing for discussions 15A-W-4


M-2 Didactic disscusion

M-3 Educational films

Food and Nutrition in Relation to Human Health

Evaluation methods (F - progressive, P - final)S-1 Short testF

S-2 Practical examF

S-3 ExamP



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_2-_??_W01Student get knowledge about the basic nutrients and theirimpact on human health.

SkillsWM-WBiHZ_2-_??_U01The student has the ability to evaluate food products and theircomposition for human development and health.

Other social / personal competencesWM-WBiHZ_2-_??_K01The student can explain the dangers associated with impropernutrition.



2,03,03,54,04,55,0


2,03,03,54,04,55,0


3,03,54,04,55,0

Required reading1. Julian E. Spallholz, Mallory Boylan, Judy A. Driskell., Nutrition: CHEMISTRY AND BIOLOGY, CRC Press, 1998, II, ISBN 0-8493-8504-0

2. Rudolf Steiner, Nutrition: Food, Health and Spiritual Development., Rudolf Steiner Press., 20063. Susan Allport, The Queen of Fats: Why Omega-3s Were Removed from the Western Diet and What We Can Do to Replace Them,University of California Press, 2006

Supplementary reading1. Geissler C, Powers H, Human Nutrition, Churchill Livingstone Elsevier, 2011

2. MJ, Lanham-New SA, Cassidy A, Vorster HH, Introduction to Human Nutrition, A John Wiley & Sons, Ltd., 2009

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-12-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Fundamentals of laboratory diagnostics






Other teachers

PrerequisitesW-1 physiology, anatomy

Module/course unit objectivesC-1 To acquaint students with the fundamental methods used in laboratory diagnostic

C-2 To acquaint students with the basic terms used in laboratory diagnostic.


T-L-1 Complete Blood Count (CBC) test. Semi-automated blood analysis.Evaluation of White Blood Cell 5

T-L-2 The urine analysis (Reader Urine Analyser). The physico-chemical and microscopic properties of theurine. Urine sediment analysis. 3

T-L-3 Biochemical tests. 2

T-L-4

The qualitative and quantitative methods in parasitology. Coproscopic techniques for detection andquantitative estimation of endoparasites. Microscopic Examination.The post-mortem parasitological examination: dissection, parasites isolation, preservation andexamination of collected samples.Detection of Trichinela in meat samples. Trichinoscopy and pool-sample digestion method.

10

T-L-5 Determination of selenium (Se) in biological samples 5

T-L-6 Laboratory diagnosis of cryptosporidiosis. 5

Student workload - forms of activity Number of hoursuczestnictwo w zajęciach 30A-L-1

an individual work, studying of literature 120A-L-2

Teaching methods / toolsM-1 laboratory

Evaluation methods (F - progressive, P - final)S-1 Continuous assessment of activities performed by student.F



study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-1

T-L-1T-L-2 M-1

WM-WBiHZ_2-_??_W01The student knows the basic terms used in laboratorydiagnostics.

T-L-3T-L-4

Skills

C-1 S-1T-L-1T-L-2T-L-3

M-1WM-WBiHZ_2-_??_U01The student is able to prepare samples of biological material,perform tests and interpret the results.

T-L-4T-L-5T-L-6

Fundamentals of Laboratory Diagnostics


C-1 S-1M-1WM-WBiHZ_2-_??_K01The student demonstrates responsibility for their own safety andothers.



2,03,0 The student knows only part of the required terms used in laboratory diagnostics.

3,54,04,55,0


2,03,0 The student is able to prepare the samples and perform tests, but needs the help of a teacher. Student presents the results,

but he/she can not assess them.3,54,04,55,0


3,0 The student demonstrates little responsibility for their own safety and others.

3,54,04,55,0

Required reading1. Pagana K., Pagana T., Mosby's Diagnostic and Laboratory Test Reference, Elservier Health Sciences, 2006

2. Garcia L., Practical Guide to Diagnostic Parasitology, American Society for Microbiology, 2009

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-13-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit General Genetics







Other teachers

PrerequisitesW-1 Basics of molecular biology and biochemistry

Module/course unit objectivesC-1 Get knowledge about inheritance of traits

C-2 Training and practice in methods using in molecular genetics

Course content divided into various forms of instruction Number of hoursT-L-1 Genetic and physical mapping 2

T-L-2 Population genetics 3

T-L-3 DNA analysis methods and their practical use 10

T-W-1 History of genetics and terms using in genetics 2

T-W-2 Inheritance of quantitative and qualitative traits 5

T-W-3 Structure of DNA and chromosomes. 2

T-W-4 Genes and genetic code 2

T-W-5 Mutations and other sources of biodiversity 2

T-W-6 Genes expression and their regulation 2


Solving and calculating problems 20A-L-2


Consultation 5A-L-4









General Genetics




study





education

Teachingmethods


methods

Knowledge

C-1 S-1T-L-1T-L-2T-W-1T-W-2

M-1WM-WBiHZ_2-_null_W01Student defines the mechanisms of traits inheritance andindicates the sources of genetic variability


Skills

C-2 S-2T-L-1T-L-2 M-2

WM-WBiHZ_2-_null_U01Student is able to solve genetic problems and gained experiencein basic molecular methods

T-L-3T-W-2


C-1C-2

S-1S-2

T-L-3T-W-1 M-1

M-2WM-WBiHZ_2-_null_K01Student is aware of benefits and dangers resulting fromachievements in modern genetics

T-W-5



2,03,0 Student knows the terms using in genetics as well as basic mechanisms of traits inheritance

3,54,04,55,0


2,03,0 Student solves easy genetic crosswords and gained experience in basic laboratory methods

3,54,04,55,0


2,03,0 Student gives a few practical examples of genetics applications

3,54,04,55,0

Required reading1. E. Passarge, Color Atlas of Genetics, Thieme Medical Publishers, 2012

2. H. Fletcher, I. Hickey, BIOS Instant Notes in Genetics, Garland Science, 2012

Supplementary reading1. J. E. Krebs, E. S. Goldstein, S. T. Kilpatrick, Lewin's GENES XI, Jones & Bartlett Publishers, 2014

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-14-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Genetic Engineering Methods







Other teachers

PrerequisitesW-1 Basics of molecular methods

Module/course unit objectivesC-1 Get knowledge about techniques used in genetic engineering

C-2 Get the practical experience in genetic analysis methods

Course content divided into various forms of instruction Number of hoursT-L-1 Using different mothods to extract nucleic acid. 3

T-L-2 Set up a PCR. 3

T-L-3 Restriction enzyme digestrin, analyze PCR product using agarose gel electrophoresis. 4

T-L-4 HRM - High- esolution melt curve analysis, RT-PCR, Real Time PCR, 5

T-W-1 Introduction: different methods used in genetic engineering and thair application. 2

T-W-2 DNA amplification methods including RT-PCR (reverse transcriptase), in situ PCR, mutational analysis. 4

T-W-3 PCR based mutation detection: SSCP, AS-PCR analysis, heteroduplex analysis, denaturing gradient gelelectiophoresis, 4

T-W-4 DNA microarrays ( DNA chips), sequencing, nucleotide enumeration. 4

T-W-5 Genetic engineering methods and ethical considetations 1












S-2 PresentationF

Genetic Engineering Methods



study





education

Teachingmethods


methods

Knowledge

Skills



Knowledge

Skills





[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-15-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Genetic Markers for Food Quality







Other teachers

PrerequisitesW-1 Basics of genetics, physiology and molecular genetics

Module/course unit objectivesC-1 To familiarize students with possibility of genetic markers use in food analysis

C-2 Practical use of DNA analysis to assess food quality

Course content divided into various forms of instruction Number of hoursT-L-1 Methodological approach for food markers detection 2

T-L-2 Food fraud detection 4

T-L-3 DNA test for lactose intolerance 4

T-L-4 Tests for "supertaster" 5

T-W-1 Introduction, history, basic terms, markers classes, criteria of markers application 2

T-W-2 Genetic markers of taste and food preferences 2

T-W-3 Methods for GMO detection in food 2

T-W-4 Application of markers in food authentication 2

T-W-5 DNA barcoding and its application in food industry 2

T-W-6Genetic markers for:•fruit and vegetables quality•milk quality and quantity•different meat species quality

5




Preparing the multimedia presentation 10A-L-4


Parcipitation to lectures 15A-W-1






Genetic Markers for Food Quality

Teaching methods / toolsM-2 Laboratory works


S-2 Assessment of multimedia presentationF




study





education

Teachingmethods


methods

Knowledge

C-1 S-1S-2

T-W-1T-W-2T-W-3

M-1WM-WBiHZ_2-_null_W01Students indicates the need and practical application of DNAmarkers in food analysis

T-W-4T-W-5T-W-6

Skills

C-2 S-3T-L-1T-L-2T-L-3 M-2

WM-WBiHZ_2-_null_U01Student gained skills in the food analysis by use DNA markersand can define the dangers associated with consumption of non-authentic food

T-L-4T-W-3T-W-4


C-1C-2

S-1S-2

T-L-1T-W-4 M-1

M-2WM-WBiHZ_2-_null_K01Student is aware of needs and benefits of DNA markersapplication by the food analysis

T-W-5T-W-6



2,03,0 Student gives few examples of DNA markers application in food analysis

3,54,04,55,0


2,03,0 Student gained basic experience in food analysis by use DNA markers and gives few examples of food fraud

3,54,04,55,0


2,03,0 Student simply explains why DNA markers are usefull in food analysis

3,54,04,55,0

Required reading1. R. Blair, J. M. Regenstein, Genetic Modification and Food Quality: A Down to Earth Analysis, John Wiley & Sons, Ltd., 2015

2. D. Sun, Modern Techniques for Food Authentication, Elsevier, 2008

Supplementary reading1. I. S. Arvanitoyannis, Authenticity of Foods of Animal Origin, CRC Press, 2015

2. S. Sforza, Food Authentication Using Bioorganic Molecules, DEStech Publications, Inc., 2013

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-16-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Genomics







Other teachers

PrerequisitesW-1 Molecular biology and genetics

Module/course unit objectivesC-1 Get knowledge about genomes structure, sizes and evolution

C-2 Get knowledge and training in methods of genomes analysis

Course content divided into various forms of instruction Number of hoursT-L-1 Isolation of plasmids and restriction mapping 6

T-L-2 Isolation of mtDNA and D-loop polymorphism analysis 6

T-L-3 Practical application of genomic databases. 3

T-W-1 Introduction - history of genomics, fields, connection with other sciences 2

T-W-2 Size and structure of pro-, eukaryotic and organelle genomes with its comparison 2

T-W-3 Origin of new genes, ole of noncoding DNA 3

T-W-4 Diseases caused by genome architecture 2

T-W-5 Physical and genetic maps 2

T-W-6 Sequencing of genes and genomes 2

T-W-7 Methods in functional genomics 2


Study of reccomended literture 15A-L-2


Preparing for classes 15A-L-4









Genomics





study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-1


M-1WM-WBiHZ_2-_??_W01Student explains the issues related to the analysis of genomicsequences including genome projects and has knowledge in thearea of the functional and comparative genomics.

T-W-5T-W-6T-W-7

Skills

C-1C-2

S-1S-2


M-1M-2

WM-WBiHZ_2-_??_U01Student perceives genome in holistic way regarding to itsstructure and function and acquired the ability to explore thedatabases containing deposited sequences and genomes data

T-W-5T-W-6T-W-7


C-1C-2

S-1S-2

T-L-3T-W-2T-W-3

M-1M-2

WM-WBiHZ_2-_??_K01Student creates an active attitude, has the ability to holisitcview on the facts and see the issues in a broader context

T-W-5T-W-6



2,03,0 Students knows basic terms in genomics, some structures and sizes of genomes and ways of genome sequencing

3,54,04,55,0


2,03,0 Student explains complexity of genomes, simply explores databases and indicates methods of genomes analysis

3,54,04,55,0


3,0 Student is aware of biological processes complexity and recognizes the role of genome within them

3,54,04,55,0

Required reading1. T.A. Brown, Genomes 3, Garland Science, 2006

2. A. Lesk, Introduction to genomics, Oxford University Press, 2012

Supplementary reading1. G. Gibson, S. Muse, A Primer of Genome Science, Third Edition, Sinauer Associates Inc., 2009

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-17-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Human Genetics







Other teachers

PrerequisitesW-1 Basics of Genetics

Module/course unit objectivesC-1 To get knowledge about inheritance of different traits, diseases and predispositions in human

C-2 Practical use of methods based on DNA analysis in human genetics

Course content divided into various forms of instruction Number of hoursT-L-1 DNA testing for chosen traits and predispositions in human 15

T-W-1 History of human genetics and milestones 2

T-W-2 Mitochondrial diseases 2

T-W-3 Model organisms in human genetics 2

T-W-4 The role of environment and genes in carcinogenesis 2

T-W-5 Ecogenetics 2

T-W-6 Genetic theories of aging 2

T-W-7 Genetics of sport performance 3



Preparing for the classess 15A-L-3











Human Genetics




study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-1

T-L-1T-W-2T-W-4

M-1WM-WBiHZ_2-_null_W01Description of genetic defects and predispositions in human andindication of practical knowledge application in human genetics

T-W-5T-W-6

Skills

C-1C-2

S-1S-2

T-L-1T-W-1 M-1

M-2

WM-WBiHZ_2-_null_U01Ability to interpret genetic data and use of acquired knowledgein daily life and in evaluation of the latest achievements in thefield of human genetics

T-W-3T-W-7


C-1C-2 S-2

T-W-1T-W-3 M-1

M-2WM-WBiHZ_2-_null_K01Awareness of the advantages and risks of the achievements ingenetics

T-W-4T-W-7



2,03,0 Student describes a few examples of genetic defects and predispositions in human and their inheritance

3,54,04,55,0


2,03,0 Student is able to interpret simple genetic data

3,54,04,55,0


2,03,0 Students is aware of the advantages and risks of the achievements in genetics on satisfactory level

3,54,04,55,0

Required reading1. Lewis R., Human Genetics, 11th Edition, McGraw-Hill Education, 2014

Supplementary reading1. Speicher M. R., Motulsky A. G., Stylianos E., Vogel and Motulsky's Human Genetics. Problems and Approaches., Springer, 2010

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-19-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Industrial Enzymology







Other teachers

PrerequisitesW-1 Basic knowlage of chemistry, biochemistry and biophysic


C-1 The purpose of the course is to teach students about technologies of industrial enzymes manufacturing and characterize theadvantages of using enzyme preparations in the industry.

Course content divided into various forms of instruction Number of hoursT-L-1 Estimation of basic catalytical parameters of enzymes with invertase form S. cerevisiae as model 6

T-L-2 Production laccase from T.versicolor 4

T-L-3 Immobilization of alpha amylase on polysaccharides carriers 3

T-L-4 Starch conversion by immobilised amylolytic enzymes for biofuel production 2

T-W-1 Principles of enzymology 6

T-W-2 Methods of enzymes production for industrial applications 2

T-W-3 Strategies for improving enzymes for industrial application 2

T-W-4 Enzymes in food industry 2

T-W-5 Enzymes in biofuel production 2

T-W-6 Enzymes in environment protection 1








M-2 disscusion



Evaluation methods (F - progressive, P - final)S-1 Presentation of projectP

Industrial Enzymology



study





education

Teachingmethods


methods

Knowledge

Skills



Knowledge

Skills


Required reading1. Jerzy Witwicki, Elementy enzymologii, PWN, Warszawa, 1984

2. David Hawcroft, Diagnostic enzymology, ACOL, Londyn, 1986

3. Wolfgang Aehle red., Enzymes in Industry: Production and Applications, Willey VCH, 2007, III





[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-18-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit In vitro and in vivo Methods in ToxicologicalAssessment of Xenobiotics








PrerequisitesW-1 no requirements

Module/course unit objectivesC-1 To acquaint students with the in vivo and in vitro methods used in assessing the toxicity of xenobiotics.

C-2 To acquaint students with the mechanisms of action of toxic substances and their metabolism.

C-3 To acquaint students with the computational methods in toxicity, exposure and risk assessment.

Course content divided into various forms of instruction Number of hoursT-A-1 Metabolism of xenobiotics. 2

T-A-2 The mechanisms of toxicity. 2

T-A-3 Methods for determining the median lethal dose/concentration (LD50 i LC50). 3

T-A-4 Calculation methods in the toxicity assessment. Exposure and risk assessment. Determination ofNOAEL, LOAEL, LoAL and RfD. 3

T-A-5 Toxicological evaluation of raw materials and cosmetic products. 2

T-A-6 Alternative methods in ecotoxicological studies. 3

T-W-1 The use of animals in toxicometric research. The main organizations promoting alternative methods inthe world. Database of in vitro techniques used in toxicology. 2

T-W-2 Use of in vivo tests in evaluation of the toxicity of chemicals. Types and directions of toxicologicalresearch. 2

T-W-3 Acute toxicity - classic and alternative methods. 4

T-W-4 Repeated dose toxicity. The methods used in assessing the genotoxicity, carcinogenicity,neurotoxicity, effects on reproduction, fertility and offspring. 3

T-W-5Evaluation of toxicity of a compound based on the relationship between the chemical structure andbiological activity (structure-activity relationship). Factors affecting the toxicity. Genetic factorsincreasing the sensitivity to chemical compounds.

2

T-W-6 Chemical safety. The most important rules governing the issue of chemical safety. The classificationand labeling of chemicals. 2



preparation for test 15A-A-3




In vitro and in vivo Methods in Toxicological Assessment of Xenobiotics

Teaching methods / toolsM-1 Delivery method, lecture/presentation.

M-2 Discussion

M-3 Explanation

Evaluation methods (F - progressive, P - final)S-1 testF

S-2 assessment of student's activity and attitudes towards discussed issues.F

S-3 reportF



study





education

Teachingmethods


methods

Knowledge

C-1 S-1T-A-6T-W-1T-W-2

M-1M-2M-3

WM-WBiHZ_2-_null_W01Student describes methods using in toxicity assessment ofxenobiotics.

T-W-3T-W-4T-W-5

C-1 S-1T-A-1T-A-2

M-1M-2M-3

WM-WBiHZ_2-_null_W02Student desribes the metabolism of toxins and mechanisms oftoxicity

T-A-5

Skills

C-3 S-3T-A-3

M-3WM-WBiHZ_2-_null_U01Student uses the computational methods in toxicity, exposureand risk assessment.

T-A-4


C-1 S-2

T-A-1T-A-2T-A-3T-A-4T-A-5T-A-6

M-1M-3

WM-WBiHZ_2-_null_K01Student understands the need to reduce the use of animals intoxicological studies.




2,03,0 The student lists and discribes the methods uses in toxicity assessment.

3,54,04,55,0


2,03,0 Student describes phases of xenobiotic's metabolism and lists mechanisms of toxicity.

3,54,04,55,0


2,03,0 Student is able to calculate: the LD50 for a specific subtance (with using at least two methods), RfD, LOAL.

3,54,04,55,0


2,03,0 Student explains the reasons for reducing the number of animals in experimental studies.

3,54,04,55,0

Required reading1. Michael Balls, Robert Combes and Andrew Worth, The History of Alternative Test Methods in Toxicology (1st edition), Elsevier, 2018

[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-20-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Medical and Veterinary Microbiology







Other teachers

Prerequisites



C-1 The course aims are to provide a comprehensive theoretical and practical knowledge of medical and veterinarymicrobiology.

Course content divided into various forms of instruction Number of hoursT-L-1 Methods of culturing clinically significant bacteria 2

T-L-2 Conditions of cultures of clinically significant bacteria 2

T-L-3 Microscopic examination of microorganisms 2

T-L-4 Detection and identification of various kind of clinically significant microorganisms 4

T-L-5 Determination of antibiotic susceptibility of pathogenic bacteria 3

T-L-6 Study of biochemical activity of clinically significant microorganisms 2

T-W-1 Information about working in clinical microbiological laboratory 2

T-W-2 Methods for determination and controlling growth of pathogenic bacteria 2

T-W-3 Detection and identification of various kind of clinically significant microorganisms 2

T-W-4 Determination of antibiotic susceptibility of pathogenic bacteria 2

T-W-5 Upper Respiratory Tract Infections 1

T-W-6 Lower Respiratory Tract Infections 1

T-W-7 Gastrointestinal Tract Infections 1

T-W-8 Genitourinary Tract Infections 1

T-W-9 Skin and Soft Tissue Infections 1

T-W-10 Immunoprophylaxis and Immunotherapy 2









Medical and Veterinary Microbiology


M-2 Laboratory






study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_2-_null_W01The student can choose the appropriate research techniques forthe isolation and identification of clinical strains of bacteria andfungi.

SkillsWM-WBiHZ_2-_null_U01The student uses skills on the methods of diagnosis, preventionand treatment of bacterial and fungal diseases.

Other social / personal competencesWM-WBiHZ_2-_null_K01The student demonstrates responsibility and awareness ofdecisions when conducting diagnostic tests and proceduresprevention and treatment of infectious diseases of humansanimals.



2,03,03,54,04,55,0


2,03,03,54,04,55,0


2,03,03,54,04,55,0




[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-21-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Methods of Monitoring the Reproductive Processes inAnimals






Stankiewicz Tomasz ([email protected])Leading teacherBłaszczyk Barbara ([email protected]), Gączarzewicz Dariusz([email protected]), Stankiewicz Tomasz ([email protected]),Udała Jan ([email protected])

Other teachers

PrerequisitesW-1 The knowledge of the biotechnology in animal reproduction.


C-1 Acquainting students with the possibility of controlling the reproductive processes in male and female taking theperformance of detection of various ailments and disorders.

C-2 Developing skills of the proper selection of methods and interpretation of the results in the evaluation of the reproductiveprocesses.


T-A-1The indirect methods for monitoring of ovarian cycle (signs of oestrus, hormonal tests, evaluation ofcervical mucus and its degree of crystallization, cytological smear evaluation, measurement of bodytemperature).

4

T-A-2 The direct methods for monitoring of ovarian cycle (laparoscopy, ultrasound). 3

T-A-3 Methods for detection and monitoring of course of the pregnancy. 3

T-A-4 The monitoring of seasonal reproductive processes. 2

T-A-5 Macroscopic evaluation of ovarian cysts and abnormalities of the reproductive organs. 3

T-W-1 The monitoring of the ovarian cycle. 4

T-W-2 The diagnostic methods used in dysfunction of the ovary (ovarian cysts, ovarian tumors). 4

T-W-3 The hormonal basis for the detection of pregnancy. Achievements in the field of the imaging course ofthe pregnancy. 4

T-W-4 The contemporary andrological diagnostic. 3

Student workload - forms of activity Number of hoursThe participation in the classes. 15A-A-1

Preparation of the presentation. 25A-A-2

Preparing to pass the exercise. 20A-A-3

The participation in the classes. 15A-W-1

The study of professional literature. 15A-W-2

The participation in the consultations. 10A-W-3

Preparing to pass lectures. 20A-W-4

Teaching methods / toolsM-1 The informative lecture with the use multimedia techniques.

M-2 Activating methods (preparation of presentations by students).

Methods of Monitoring the Reproductive Processes in Animals

Evaluation methods (F - progressive, P - final)S-1 The evaluation of presentations prepared by students (teamwork).F

S-2 The final test covering the range of content lectures.P

S-3 The final test covering the range of exercise program content.P



study





education

Teachingmethods


methods

Knowledge

C-1C-2

S-1S-2S-3

T-W-1T-W-2 M-1

M-2

WM-WBiHZ_2-_null_W01The student knows the methods for monitoring the ovariancycle, taking into account the various phases of this cycle andits potential disorders. He knows the basics of hormonalmethods for the detection of pregnancy.

T-W-3

C-1C-2 S-2

T-W-4M-1

WM-WBiHZ_2-_null_W02The student knows the current methods of used in theandrological diagnosis.

Skills

C-2 S-2S-3

T-A-1T-A-2T-A-3T-A-5

M-1WM-WBiHZ_2-_null_U01The student should be able to apply an appropriate method formonitoring the ovarian cycle and pregnancy. He is able tointerpret indicator parameters of disorders in the reproduction.

T-W-1T-W-2T-W-3

C-1C-2 S-2

T-W-4M-1

WM-WBiHZ_2-_null_U02The student is able to interpret of parameters of the clinicalevaluation in the male reproduction.


C-2

T-W-1T-W-2

M-1

WM-WBiHZ_2-_null_K01The student will be able to apply the acquired knowledge andskills for the proper selection of and interpretation of the resultsin the evaluation of the reproductive processes. The completionof the course will be helpfull for the future work in the veterinaryand medical laboratories.

T-W-3T-W-4



2,03,0 The student lists the methods for monitoring the ovarian cycle and pregnancy diagnosis, but he can not describe them.

3,54,04,55,0


2,03,0 The student lists the methods used in the evaluation of the semen.

3,54,04,55,0


2,03,0 The student can choose some methods for monitoring the ovarian cycle and pregnancy diagnosis.

3,54,04,55,0

WM-WBiHZ_2-_null_U02

2,03,0 The student is able interpret only some parameters in the clinical evaluation of the male reproduction.

3,54,04,55,0


2,03,0 The student is oriented in the discussed topics, but he showed little independent activity in the discussion. He can work in a

group.3,54,04,55,0

Required reading1. Hafez E.S.E., Hafez B., Reproduction in farm animals, Lippincott Williams & Wilkins, Philadelphia (U.A), 2000

2. T. A. McGeady, P. J. Quinn, E. S. FitzPatrick, M. T. Ryan, Veterinary Embryology, Blackwell Publishing, 2006

Supplementary reading1. Gordon I. R., Reproductive technologies in farm animals, CABI Pub, Wallingford, Oxfordshire, Cambridge, MA, 2004

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-22-Z

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Molecular Biology







Other teachers

PrerequisitesW-1 Basics of molecular genetics

Module/course unit objectivesC-1 Get knowledge about molecular gene organization


Course content divided into various forms of instruction Number of hoursT-L-1 Extraction and purification of cellular RNA 3

T-L-2 Gel electrophoresis to check RNA. 2

T-L-3 PCR- clean up and cloning reaction 3

T-L-4 Primer design, CAPS search. 2

T-L-5 Genomic sequence analysis: gene finding, BLAST searching, genome annotation. 3

T-L-6 DNA sequence analysis - cloning strategies, computer-assisted restriction analysis. 2

T-W-1 Introduction: History of molecular biology, DNA as the genetic material, nucleic acid structure,hybridization. 2

T-W-2 DNA replication, bacterial and eucaryotic DNA polymerases. 2

T-W-3 Gene structure, replication, transcription, translation. 4

T-W-4 RNA processing: splicing, spliceosomes, snRNPs, self splicing introns, polyadenylation. 4

T-W-5 Eucaryotic transcriptional regulation, transposons, recombination. 3












Molecular Biology


S-2 PresentationF



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_2-_null_W01Understanding of molecular mechanisms of genome functioning

SkillsWM-WBiHZ_2-_null_U01Ability to differentiate basic processes ongoing in a living cell

Other social / personal competencesWM-WBiHZ_2-_null_K01Teaching and explaining of basic molecular processes ongoingin cells of living organisms



2,03,03,54,04,55,0


2,03,03,54,04,55,0


2,03,03,54,04,55,0

Required reading1. Weaver R., Hill M.G., Miolecular Biology, 2001

2. Watson J.D., Molecular Biology of the gene, Pearson Education, 2013

Supplementary reading1. Albertis B et all., Molecular Biology of the Cell, Garland Publishing, New York, 2009

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-23-Z

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Molecular Diagnostics







Other teachers

PrerequisitesW-1 Basic knowledge of molecular technique.

Module/course unit objectivesC-1 Acquaint the students to versatile tools and techniques employed in diagnostic molecular and recombinant DNA technology.

Course content divided into various forms of instruction Number of hoursT-L-1 Preventing contamination, DNA extraction, asses purity of DNA 3

T-L-2 Application of DNA testing. preparation the samples to analysis. 3

T-L-3 Molecular laboratory diagnostic of different genetic deseases. 7

T-L-4 Analysis of results 2

T-W-1 Nucleid acid structure, extraction and probe preparation. 3

T-W-2 Manipulation DNA sequences with versatile DNA modifying enzymes. 3

T-W-3 DNA amplification methods, mutational analysis, sample preparations. 3

T-W-4 Alternative methods for amplified nucleic acid testing 2

T-W-5 Genes therapy, applications in diagnostic of genetic disorden, human genome project. 4

Student workload - forms of activity Number of hoursParticipation in laboratories 15A-L-1











S-2 PresentationF

Molecular Diagnostics



study





education

Teachingmethods


methods

Knowledge

Skills



Knowledge

Skills


Required reading1. Bruns D.E, Ashwood E.R., Burtis C.A., Fundamentals of molecular diagnostic, 2011

2. Coleman W.B., Molecular Diagnostic, Springer, 2005

Supplementary reading1. Debnath M.,, Molecular diagnostic: Promises and possibillities, Springer, Netherland, 2010

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-24-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Molecular Modeling of Enzymes







Other teachers

PrerequisitesW-1 Knowledge of organic and inorganic chemistry, biochemistry, biophysics, English at intermediate level,

Module/course unit objectivesC-1 Developing skills of selection of appropriate tools to solve and analyze the structure of enzymes

Course content divided into various forms of instruction Number of hoursT-L-1 Analysis of enzymes structural properities by molecular modeling software 6

T-L-2 Prediction of tretairy structure of alpha - amylase form A. niger 4

T-L-3 Modeling of catalytic properities of alpha - amylase from A. niger 5

T-W-1 Methods and source of obtaining information about the structure of enzymes 1

T-W-2 Methods of functional analysis of the primary structure of enzymes 2

T-W-3 Methods of prediction and analyze the secondary structure of enzymes 2

T-W-4 In silico methods to prediction and analyze the tretiary structure of enzymes 6

T-W-5 Methods for prediction and modeling functional properities of enzymes 4








M-2 disscusion



Evaluation methods (F - progressive, P - final)S-1 projektF

S-2 projektP

Molecular Modeling of Enzymes



study





education

Teachingmethods


methods

Knowledge

Skills



Knowledge

Skills


Required reading1. Arieh Warshel, Computer Modeling of Chemical Reactions in Enzymes and Solutions, Wiley, 1997

2. Huzefa Rangwala, George Karypis, Introduction to Protein Structure Prediction: Methods and Algorithms, 2010



5. Wolfgang Aehle red., Enzymes in Industry: Production and Applications, Willey VCH, 2007, III



[ logo uczelni ]

Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-25-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Proteomics






Herosimczyk Agnieszka ([email protected])Leading teacherDratwa-Chałupnik Alicja ([email protected]), Herosimczyk Agnieszka([email protected]), Lepczyński Adam ([email protected]),Michałek Katarzyna ([email protected]), Ożgo Małgorzata([email protected])

Other teachers

PrerequisitesW-1 Basic of the cell biology and the protein biochemistry.

Module/course unit objectivesC-1 Theoretical and practical knowledge of gel-based and chromatographic protein separation techniques.

C-2 The ability of the participants to use advanced bioinformatic tools to analyse proteomic data (1-D and 2-D gels, massspectra).

C-3 Practical use of MALDI-TOF MS (matrix-assisted laser desorption/ionisation time of flight mass spectrometer) for proteinidentification.

Course content divided into various forms of instruction Number of hoursT-L-1 Sample preparation techniques for proteomic analysis. 2

T-L-2 Protein separation using two-dimensional electrophoresis (2-DE). 2

T-L-3 Protein separation using SDS-PAGE (1-DE). 2

T-L-4 Protein gel staining methods. 2

T-L-5 Identification of proteins using mass spectrometer MALTI-TOF. 3

T-L-6 Identification of proteins using Western-blot technique. 2

T-L-7 1-DE and 2-DE gel image acquisition and bioinformatic analysis. 2

T-W-1 Introduction to proteomics. Biological significance of post-transcriptional and post-translational proteinmodifications. Proteome organization. The general principles of proteomic analysis. 2

T-W-2Gel-based protein separation techniques. The components of resolving gel matrix. Sodium-dodecylpolyacrylamide gel electrophoresis (SDS-PAGE), the principle and application of native PAGEelectrophoresis. Two dimensional electrophoresis (2-DE) – the principle of the method, samplepreparation for 2-DE, IPG strips, isoelectric focusing.

2

T-W-3Protein detection methods: coomassie stain, silver stain, negative ion staining (copper, zinc),autoradiography, fluorography, fluorescent staining. Two-dimensional difference in gel electrophoresis(2D-DIGE) – the principle and application of the method. Image acquisition and analysis of 1-D and 2-Dgels. 1-D and 2-D gels analysis softwares.

2

T-W-4 Application of mass spectrometry (MS) for protein identification. Ionization methods in massspectrometry. Types of mass analyzers. Peptide mass fingerprinting (PMF). 2

T-W-5Chromatographic methods for protein separation. Liquid chromatography (LC). Two-dimensional liquidchromatography (2-D LC). The proteomic strategies based on liquid chromatography: LC-MS, LC-MS/MS, multidimensional LC-MS/MS. Affinity chromatography (AC).

2

T-W-6 Identification of proteins using Western-blot technique. Sample preparation. Methods of proteintransfer. Incubation with antibodies. Visualisation. 3

T-W-7 Branches of proteomics: structural, functional and clinical. 2

Student workload - forms of activity Number of hours

Proteomics

Student workload - forms of activity Number of hoursParticipation in laboratories. 15A-L-1

Self work with publications. 15A-L-2

Laboratory project preparation. 20A-L-3






Teaching methods / toolsM-1 Theoretical lectures.

M-2 Discussion during laboratory classes.

M-3 Project preparation.

Evaluation methods (F - progressive, P - final)S-1 Project presentation in the writing form.F

S-2 Writing test.P



study





education

Teachingmethods


methods

Knowledge

C-1C-2 S-2


M-1WM-WBiHZ_2-_null_W02Student can enumerate and describe commonly usedtechniques used in the study of proteins.

T-W-5T-W-6T-W-7

Skills

C-1C-3 S-1

T-L-1T-L-2T-L-3T-L-4

M-2M-3

WM-WBiHZ_2-_null_U01Student is able to use commonly known proteomic techniquessuch as: 1-DE, 2-DE, MALDI-TOF MS and Western-blot.

T-L-5T-L-6T-L-7


C-1C-2C-3

S-1S-2

T-L-1T-L-2T-L-3T-L-4T-L-5T-L-6T-L-7

M-1M-2M-3

WM-WBiHZ_2-_null_K01Student is aware that there is a number of methods to analysethe different levels of protein changes in response to variousphysiological/patophysiological stimmuli in the biologicalmaterial.

T-W-1T-W-2T-W-3T-W-4T-W-5T-W-6T-W-7



2,0

3,0- student has a basic knowledge in the frame of the material program- student exerts low attitude to knowledgein the frame of expressing knowledge student makes a lot of mistakes

3,54,04,55,0


2,03,0 Student, with a help of a teacher is able to meet the challenges of preparing an appropriate laboratory protocol.

3,54,04,55,0


2,03,0 Student shows a moderate interest in participating in a verbal discussion with the teacher and colleagues during the classes.

3,54,04,55,0

Required reading1. Sheehan D., Tyther R. (Ed.), Two-dimensional electrophoresis protocols., Humana Press, New York, 2009

2. Garfin D., Ahuja S. (Ed.), Handbook of isoelectric focusing and proteomics., Elsevier Academic Press, Amsterdam, 2005

3. Walker J.M. (Ed.), second edition, The proteomics protocols handbook., Humana Press, New Jersey, 2002

Required reading4. Rabilloud T. (Ed.), .). Proteome research: two-dimensional gel electrophoresis and identification methods., Springer, Berlin, 2000

5. Hames B.D. (Ed.), third edition, Gel electrophoresis of proteins: a practical approach., Oxford University Press, England, 1998

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-26-Z

4,0

credits french

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Protéomique






Ożgo Małgorzata ([email protected])Leading teacherHerosimczyk Agnieszka ([email protected]), Lepczyński Adam([email protected]), Ożgo Małgorzata ([email protected])Other teachers

PrerequisitesW-1 la connaissance de la biochimie, de la biologie moleculaire, la genetique


C-1La Protéomique a pour objectif la formation d’étudiants capables d’identifier et/ou dedéterminer la structure de molécules biologiques simples ou complexes de toute nature (protéine, acide nucléiques,lipides), soit dans le cadre d’une démarche analytique, soit dans le cadre d’une démarche d’analyse globale dumétabolisme.


T-L-1Electrophorèse en gel de polyacrylamide contenant du dodécylsulfate de sodium (SDS-PAGE), leprincipe et les applications de l'électrophorèse sur gel natif PAGE. Electrophorèse bidimensionnelle (2-DE) – principe de la méthode, préparation des échantillons pour la 2-DE, bandes d'IPG (IPG strips),focalisation isoélectrique.

4

T-L-2

Méthodes de détection des protéines: coloration au Bleu de Coomassie, coloration à l'argent, colorationinverse avec des ions (cuivre, zinc), autoradiographie, fluorographie, coloration fluorescente. Analysedifférentielle sur un gel unique (two-dimensional difference in gel electrophoresis 2D-DIGE) – principeet applications de la méthode. Acquisition d'image et analyse de gels 1D et 2D. Logiciels d'analyse desgels 1D et 2D.

4

T-L-3 Utilisation pratique du MS MALDI-TOF (spectromètre de masse matrix-assisted laserdesorption/ionisation time of flight) pour l'identification de protéines 7

T-W-1Introduction à la protéomique. Importance biologique des modifications post-transcriptionnelles etpost-translationnelles des protéines. Organisation du protéome. Les principes généraux de l'analyseprotéomique. Techniques de séparation des protéines basées sur gel. Les composants de la matrice dugel de séparation.

4

T-W-2

Applications de la spectrométrie de masse (MS) pour l'identification des protéines. Méthodesd'ionisation en spectrométrie de masse. Types d'analyseurs de masse. Cartographie peptidiquemassique (peptide mass fingerprinting PMF). Méthodes chromatographiques pour la séparation desprotéines. Chromatographie en phase liquide (LC). Chromatographie liquide bidimensionnelle (2D LC).Les stratégies protéomiques basées sur la chromatographie liquide : LC-MS, LC-MS/MS, LC-MS/MSmultidimensionnelle. Chromatographie d'affinité. Types de protéomique : structurelle, fonctionnelle etclinique.

7

T-W-3 Techniques de séparation des protéines basées sur gel. 4

Student workload - forms of activity Number of hoursparticipation en classe 15A-L-1

préparation pour les classes 10A-L-2

consultations 10A-L-3

étude de la litérrature recommandée 15A-L-4

préparation du protocol 10A-L-5

participation en classe 15A-W-1

Prot?omique

Student workload - forms of activity Number of hoursétude de la litérrature recommandée 20A-W-2

consultations 22A-W-3

test écrit 2A-W-4

Teaching methods / toolsM-1 présntation oral

M-2 travaux pratiques

Evaluation methods (F - progressive, P - final)S-1 test écritF



study





education

Teachingmethods


methods

Knowledge

C-1 S-1T-W-1T-W-2 M-1

WM-WBiHZ_2-_null_W01l'élève peut énumérer et décrire les techniques courammentutilisées dans l'étude des protéines

T-W-3

Skills

C-1T-L-1T-L-2 M-1

M-2

WM-WBiHZ_2-_null_U01l'étudiant est capable d'utiliser des techniques protéomiquescommunément connues comme: MALDI-TOF, 2DE, WesternBloting

T-L-3


C-1

T-L-1T-L-2T-L-3 M-2

WM-WBiHZ_2-_null_K01L'étudiant est conscient qu'il existe un certain nombre deméthodes pour analyser les différents niveaux de protéines enréponse à diverses stimulations physiologiques dans le matérielbiologique

T-W-1T-W-2T-W-3



2,0

3,0l'étudiant a une connaissance de base dans le cadre du programme matérielles étudiants ont une faible attitude vis-à-vis des connaissancesdans le cadre de l'expression des connaissances, l'étudiant fait beaucoup d'erreurs

3,54,04,55,0


2,03,03,54,04,55,0


2,03,03,54,04,55,0

Required reading1. Sheehan D., Tyther R. (Ed.)., Two-dimensional electrophoresis protocols, Humana Press, New York, 2009

2. Garfin D., Ahuja S. (Ed.)., Handbook of isoelectric focusing and proteomics., Elsevier Academic Press, Amsterdam, 2005

3. Walker J.M., The proteomics protocols handbook, Humana Press,, New Jersey, 2002

Supplementary reading1. Rabilloud T., Proteome research: two-dimensional gel electrophoresis and identification methods, Springer, Berlin, 2002

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Field of study

Mode of study


ECTS


Code

Electives

Language

Elective group


stationary

WBiHZ-2-27-L

4,0

credits english

ECTS (forms) 4,0

Level second cycle

Area(s) of study


Module

Course unit Vaccinology







Other teachers

PrerequisitesW-1 Basic lab knowledge and skills.

W-2 Ability to pipet, make solutions and dilutions and to execute protocols which require the use of sterile techniques.

W-3 Basic knowledge of microbiology and immunology.


C-1 The course aims are to provide a comprehensive theoretical and practical knowledge of vaccinology, including theproduction of the vaccines.

Course content divided into various forms of instruction Number of hoursT-L-1 Preparation of vaccine 10

T-L-2 Evaluation of prepared vaccine 5

T-W-1 Immunological aspects of vaccines 3

T-W-2 Composition and types of vaccines 3

T-W-3 Vaccination of humans and animals 3

T-W-4 Methods for the preparation of vaccines 3

T-W-5 Vaccines for tomorrow 3










M-2 Laboratory

Evaluation methods (F - progressive, P - final)S-1 Writing testF



Vaccinology



study





education

Teachingmethods


methods

KnowledgeWM-WBiHZ_2-_null_W01The student knows the immunological basics related to theproduction bioproducts, knows the role of adjuvants and carriersfor synthetic vaccines, knows the rules of prevention andtreatment of certain human and animal diseases using vaccinesand immunomodulators or autovaccines.

SkillsWM-WBiHZ_2-_null_U01Student is able to classify the vaccine and analyze the reactionsof the immune system after immunization.




2,03,03,54,04,55,0


2,03,03,54,04,55,0


Required reading1. L. M. Prescott, Microbiology, McGraw-Hill Science, 2002

2. C. L. Gyles, J. F. Prescott, J. G. Songer, C. O. Thoen, Pathogenesis of Bacterial Infections in Animals 4th Ed, Blackwell Publishing, 2010

3. Roitt I., Brostoff J., Male D., Immunology, Brema, 1998

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