evaluation of pork quality a thesis
TRANSCRIPT
COMPARISON OF WARNER-BRATZLER SHEAR,
ARMOUR TENDEROMETER, AND SENSORY
EVALUATION OF PORK QUALITY
by
SHU-SHUN JULIE YEH, B.S.
A THESIS
IN
FOOD AND NUTRITION
Submitted to the Graduate Faculty of Texas Tech University in Partial Fulfillment of the Requirements for
the Degree of
MASTER OF SCIENCE
IN
HOME ECONOMICS
Approved
Accepted
December, 1973
/9 93
ACKNOWLED GMENTS
My earnest appreciation is expressed to Mrs. Clara
M. McPherson for her advice, patience, and encouragement
throughout the study. Appreciation is also extended to
Dr. S. P. Yang and Dr. Leland F. Tribble for their service
on the committee and their valuable evaluations and sug
gestions.
Acknowledgement is expressed to Mr. Charles T.
Gaskins for his guidance in statistical analysis of data.
I am deeply indebted to the members of the sensory panel
for their cooperation and help throughout the experiment.
I am grateful to those individuals in the Department of
Animal Science who were involved with this project, for
only with their help was this study possible.
1 1
TABLE OF CONTENTS
« • ACKNOWLEDGMENTS ii
LIST OF TABLES v
LIST OF ILLUSTRATIONS vi
I. INTRODUCTION 1
Statement of the Problem 1
Purposes 3
Hypotheses 3
II. REVIEW OF LITERATURE 5
Trends in Pork Consumption 5
Factors Which Affect Pork Quality 7
Genetic Factors 7
Ration and Management 8
Sex 11
Consumer's Criteria for High Quality Pork . . 12
Mechanical Measurements of Tenderness . . . . 14
III. METHODOLOGY 18
Animals 18
Ration 18
Marbling 19
The Sample 19
Cooking Procedure 19
Sensory Evaluation 20
Warner-Bratzler Shear 22
Total Fat Content 22
iii
IV
IV. FINDINGS AND INTERPRETATION 24
Variation Caused by Treatment 24
Variation in Cooking Losses 24
Sensory Panel Evaluation 25
Objective Measures of Tenderness 25
Intramuscular Fat 26
Variation Caused by Marbling 26
Variation in Cooking Losses 29
Sensory Panel Evaluation 29
Objective Measures of Tenderness 30
Intramuscular Fat 31
Variation Caused by Sex 31
Variation in Cooking Losses 31
Sensory Panel Evaluation 32
Objective Measures of Tenderness 32
Intramuscular Fat 32
Correlation Among Tenderometer, Warner-Bratzler Shear, Sensory Tenderness Scores and Number of Chew-Counts 34
Correlation Coefficients Between
Selected Variables 35
V. SUMMARY AND CONCLUSIONS 38
LIST OF REFERENCES 41
APPENDIX 46
LIST OF TABLES
Table Page
1. Least Square Means for Variables Due to Treatment 27
2. Least Square Means for Variables Due
to Marbling 28
3. Least Square Means for Variables Due to Sex . . 33
4. Correlation Coefficients Between Tenderometer, Warner-Bratzler Shear, Sensory Tenderness and Chew-Count Scores 35
5. Correlation Coefficients Between Selected Variables 36
LIST OF ILLUSTRATIONS
Figure Page
1. Pork Roast Divided into Sections for Sampling 21
VI
CHAPTER I
INTRODUCTION
Statement of the Problem
Consumers have become increasingly sensitive to fat
in their diets, with a decided objection to pork fat (1) .
This sensitivity is attributed to an increased awareness of
the health aspects of obesity and to various dietary fac
tors associated with heart disease. In addition, there has
been an increased use of vegetable oils, which has changed
the relationship of lard prices to prices of live hogs when
compared to former years. However, consumers have demon
strated a willingness to pay a higher price for lean pork
in general (2).
Swine production is an important segment of the
agriculture of the High Plains region of Texas (3). Tra
ditionally the ration fed to swine is a corn-soybean mix
ture. In the major pork producing areas of the United
States, namely the Midwest, corn has been the most common
grain used in swine rations (4). However, in Texas, corn
is not grown in as great a quantity as grain sorghum and
wheat due to climatic conditions. In 1972, grain sorghum
production in Texas was 74 million bushels, wheat 105 mil
lion bushels, and corn 6.3 million bushels (5). Therefore,
r
it is not only convenient but also economical and agricul
turally sound to substitute sorhgum and wheat for corn in
swine rations on the High Plains. Some research has been
done to determine the effect of feeding grain sorghum upon
the quality of pork (6).
Studies have shown that hog carcasses vary widely
with respect to the proportions of fat to lean (7). Diet
has a marked influence on the ratio of fat to lean in the
carcass and particularly on the level of intramuscular fat
(8). Results of work by Harrington and Pearson give sup
port to the supposition that pork loins with low levels of
intramuscular fat tend to be somewhat tougher on the aver
age, than those with more intramuscular fat, although the
relation was not sufficiently correlated to be used for
predictive purposes (9). Other factors such as sex, feed
restrictions, and level of dietary protein intake were
shown to influence tenderness (8).
Tenderness is probably the most important factor
affecting the consumer's evaluation of meat quality and
acceptability. Various mechanical methods of measuring
tenderness have been developed and evaluated (10) . The
latest device for evaluating tenderness is known as the
Armour Tenderometer which can be used on both raw and cooked
samples (11). The Warner-Bratzler Shear Apparatus, if used
to determine tenderness on raw sample, destroys its integ
rity so the same sample cannot be tested again after cooking
Further research is needed to determine the effect
of ration, sex, and level of marbling upon the quality of
pork. Such research would be highly beneficial to both the
grain and swine producers on the High Plains of Texas.
Purposes
This study was initiated between the Departments
of Animal Science and Food and Nutrition to determine the
following:
1. the differences in pork quality which will occur as a result of feeding rations formulated with grain sorghum, wheat and corn
2. the effect on pork quality when three levels of marbling are produced by feeding the above rations
3. the effect of sex on pork quality with ration and marbling as variables
4. the relationships between Armour Tenderometer scores, Warner-Bratzler shear values, and sensory panel chew counts.
Hypotheses
The following hypotheses were tested:
1. There will be no significant differences in
the quality of the pork which can be attributed to type
of ration.
2. There will be no significant differences in
the quality of the pork which can be attributed to sex
of the animals.
3. There will be significant differences in the
quality of the pork which can be attributed to level of
marbling.
4. There will be significant relationships between
the Armour Tenderometer values, Warner-Bratzler shear values
and sensory panel chew counts.
5. The pork roasts with higher levels of marbling
will show greater intramuscular fat content when chemically
analyzed.
CHAPTER II
REVIEW OF LITERATURE
Trends in Pork Consumption
The per capita pork consumption in 1879 was estimated
to be 73 lbs.; whereas, in 1966 the USDA estimated it to be
only 58 lbs. with the exception of the drought years of
1935-36, the 1966 per capita consumption was the lowest ever
recorded, in spite of the fact that the per capita consump
tion of all meats was the second highest in the nation's
history at 170 lbs. Thus Americans are eating more meat
but less pork. There are four possible explanations for
this: 1) proliferation of alternatives; 2) increased protein
competition; 3) changes in consumer attitudes toward obesity
and heart disease; and 4) the image of pork (12).
The development of new products to satisfy growing
consumer demands is a reflection of material progress.
Thousands of separately coded sizes, brands, flavors and
types of items are available. The relative decline in pork
consumption may be due partially to this proliferation of
food items but not entirely since the per capita consump
tion of beef and sugar has doubled and that of poultry and
cheese has tripled (12).
The USDA report of 1966 showed that 79% more beef
was consumed per person than pork. Other animal protein
sources which showed an increased consumption in the last
decade were poultry 85%, breaded shrimp 120%, and fish
sticks 53%, However, pork showed a 3% loss, even though
population had increased 19% (12).
The threat to pork consumption also may be attrib
uted to the development of spun soy protein products which
already are playing an increasingly important role in the
consumer's diet. Soy protein is roughly half the cost of
animal protein and has the ability to provide a wide range
of texture, flavor and appearance (13) , Thus it is reason
able to conclude that vegetable proteins will make further
intrusions into the market for pork products.
Recorded history has viewed obesity as a prestigious
sign of affluence since only the prosperous could afford
enough food to provide the calorie surplus that results in
obesity. However, consumer attitudes toward obesity have
changed radically because of increased medical knowledge
and a substantial change in education level (14, 15, 16, 17,
18, 19). Diet is now considered to be one of the factors
which can be manipulated to aid in the prevention and con
trol of heart disease (20). The publicity about the role
of saturated animal fat in arteriosclerosis has condemned
pork unnecessarily, when actually pork fat is less satu
rated than that of beef and lamb (21). With proper selec
tion and trimming, pork can be among the leanest of meats,
but the average consumer does not perceive pork to be lean.
It is difficult to understand why the producer, the packer
and the retailer do not produce what people want to buy
rather than sell them what happens to be produced (12).
The last reason why people are eating relatively
less pork is due to the image that pork presents as a food.
The so-called uncleanness of the pig and the vague fear of
certain diseases long have been associated with pork (12).
There are also numerous biblical references forbidding the
consumption of pork (22) . Such ideas have pervaded and
have conditioned every consumer since childhood.
Factors Which Affect Pork Quality
It has been stated that pork carcass quality is
dependent upon genetics, nutrition and management. It is
relatively simple to turn a good pig into a poor carcass
and a relatively poor pig into a reasonably good carcass
by the appropriate management systems (8).
Gentic factors
Considerable research has been conducted on the
inheritance of the more standard pork carcass evaluation
measurements (length, backfat thickness, loin eye area,
and lean cut yield). These heritable traits are rather
well established, but research regarding the inheritance
of quality aspects of pork muscle are more limited (23,
24, 25). However, based on the data available at present,
it appears that most measures of quality are moderately to
highly heritable. The heritability estimates tend to be
8
lower than most estimates for carcass length, backfat thick
ness, and loin eye area, but apparently are sufficiently
high to justify selection in a breeding program. The data
available indicate that superior meat type hogs with ac
ceptable quality can be produced. However, lean meat syn
thesis by pigs is genetically controlled and genetic poten
tial cannot be exceeded by feeding excessive nutrients (8).
Ration and management
Rapidity of gain in pigs and carcass characteristics
are genetically controlled, but each may be greatly influ
enced by the balance of nutrients and the rationing of the
diet. Any essential nutrient may directly or indirectly
affect carcass measurements through its influence on rate
or pattern of growth. Nutritional deficiencies, which re
sult in an extended and marked reduction in growth rate
followed by ad libitum intake of an adequate diet, may re
sult in excessive fat deposition and results in an animal
with a high ratio of fat to lean in the carcass. The most
common and readily demonstrated effect of nutrition on car
cass characteristics relate to energy intake, kinds and
amounts of amino acids, and protein to energy ratio in the
diet (8).
Braude et_ al . (26) established that feed restriction
resulted in a reduction in rate of gain and backfat thick
nesses and in size of loin eye. Restricting the feed intake
during the finishing stage resulted in a higher ratio of
lean to fat in the carcass, depending upon degree of re
striction and length of time restriction was imposed. Die
tary protein levels and energy levels have a much greater
influence on intramuscular fat deposition than on backfat
thickness, percent of lean cuts or performance character
istics. Wagner e_t aj . (27) found that fat in the longis-
simus dorsi of pigs finished on a 12% protein ration aver
aged 16.3% fat, whereas that of pigs finished on 16% pro
tein averaged only 9.3%,
Even greater differences in intramuscular fat may
be demonstrated by varying the protein and energy levels.
Pigs fed high energy-low protein diets had twice the amount
of fat in the longissimus dorsi as did animals fed a low
energy, high protein diet (8).
The chemical composition of dietary fat greatly
affects the composition of pork carcass fat. The pig con
verts excess carbohydrate to saturated or monounsaturated
fatty acids of 16 or 18 carbon chains. Thus the poly
unsaturated fatty acids in the carcass are primarily of
dietary origin (8).
Dahl (28) found that the fat present in barley or
in oats had a measurable effect on the linoleic acid con
tent of carcass fat. Research with corn and barley diets
also indicated retention of a high proportion of the die
tary linoleic acid.
10
Though barley is low in fat and contains less than
0.3% linoleic, if the pig is fed barley to market weight,
the fat in the carcass contains much higher levels of
linoleic acid, Oats are both higher in linoleic acid and
lower in digestible carbohydrates and yield a carcass
markedly higher in linoleic acid, a combined result of the
deposition of dietary fatty acids and the reduced synthesis
of saturated fatty acids from carbohydrates. These results
illustrate how readily the composition of pork fat may be
influenced by dietary variations (29).
Swine, because their digestive system can make only
limited use of forage, must be fed chiefly concentrates.
The cereal grains, rich in total digestible nutrients and
net energy are therefore desirable for feeding stock.
Among the various grains, corn, wheat and the grain sor
ghums are leading in nutritive value. Though grains are
easily digested, they are relatively low in protein and
poor in protein quality. In feeding swine, protein sup
plements of good quality are fed in addition to grain to
obtain efficient results (4).
Corn ranks far ahead of any other cereals in im
portance for livestock feeding in the United States. About
90% of the huge corn crop is fed to farm animals. Corn is
highest among the grains in total digestible nutrients and
net energy, and is probably the most palatable of the
cereals for most farm stock (4).
11
Grain sorghum is used principally for livestock
feeding in the United States. It is most widely grown in
areas where corn is not successful, because it is well
tolerant of heat and drought. In the southern Great Plains
states of Texas, Kansas, Oklahoma and New Mexico the sor
ghums are of great value (4, 32). In 1972, Texas grain
sorghum production was 74,197,000 bushels, which was about
half of the nation's production (5),
Wheat is grown mostly for the manufacture of flour
and other human foods. However, wheat is satisfactory for
all classes of stock when properly used, and is equal or
nearly equal to corn in feeding value (4). Some experi
ments have shown that pigs fed wheat gained slightly faster
than those fed corn (30).
Sex
Barrows gain somewhat faster and finish markedly
fatter at a given weight than gilts. Gilts gain more ef
ficiently and produce longer, leaner, and more heavily
muscled carcasses (31). Pigs that were grown during the
fall and winter were more highly marbled than those grown
in spring and summer. Sex differences in marbling ap
proached significance (P<.05) with barrows exhibiting
more intramuscular fat (32).
Wallace (33) showed that chops from barrows were
more tender than chops from gilts, and chops from pigs fed
12
the low-protein diet were far more tender than those from
pigs fed adequate protein. Heretofore, tenderness in pork
has not been a very important consideration since animals
are usually quite young when slaughtered and differences
in tenderness do not approach those seen in beef. However,
it is well to understand that factors such as sex, feed
restriction, and level of dietary protein intake can influ
ence tenderness markedly.
Consumer's Criteria for High Quality Pork
Knowledge of the factors that are related to the
quality of pork is important. Even though the quality of
fresh meat has a substantial influence upon its acceptance,
the final judgement is based upon the palatability of the
cooked meat (34).
According to Kramer (35) the overall quality of
food can be divided into three main categories: quanti
tative, hidden, and sensory. The quantitative aspect, i.e.
yield of the product as a result of raw material, is mainly
the concern of the producer and the processor. The hidden
aspect, i.e. nutritive value, and the sensory attributes
are both related to quality and are the primary concern of
the consumer. Sensory attributes of food include: 1) ap
pearance, which includes color, size, shape and absence of
defects; 2) kinesthetics, which may include texture, con
sistency or viscosity; and 3) flavor, which involves taste
and odor (36).
13
Pork quality as measured by consumer acceptability
includes tenderness, flavor, juiciness (10, 37, 38, 39,
40), color (10, 37), and marbling (37, 40, 41).
Hinnergardt (42) indicated that marbling score,
grade, and loin weight have been advanced as the means by
which the tenderness of the cooked meat could be predicted
from the raw state. Marbling level was significantly af
fected by sex (41), and was significantly correlated with
meat firmness (38). Wellington (43) also indicated that
the higher the level of marbling in beef, the more tender
it would be, A moderate amount of marbling in pork pro
vides an acceptable product (32). The sensory juiciness
and tenderness of lean pork also are correlated to marbling
levels (44).
Tenderness is probably the most important factor
affecting consumer evaluation of meat quality and accepta
bility. In testing the tenderness of meat using a sensory
panel, several components of tenderness have been separated
to use as a guideline. These include: 1) the ease with
which teeth sink into the meat, or softness; 2) the crumb-
liness of the muscle fibers; 3) the amount of connective
tissue or the amount of residue remaining after the meat
is chewed for a certain time; and 4) the time and effort
required to masticate a given portion in the mouth as de
termined by the number of chews required (9).
14
Mechanical measurements of tenderness
According to the mode of action, the mechanical
methods of measuring tenderness involve four different
types: 1) shear; 2) penetration; 3) biting; and 4) minc
ing (36), The Warner-Bratzler Shear Apparatus represents
one of the most commonly used and accepted mechanical de
vices to evaluate consumer acceptance of meat (45, 46) ,
Warner-Bratzler shear values have been found to be highly
significantly correlated with panel tenderness scores
(37, 40, 45),
Most methods mechanically determining tenderness
on a raw sample destroy its integrity so that the same
sample cannot be meaningfully tested again. One of the
most successful methods to relate mechanical measures of
raw meat tenderness to cooked meat tenderness is the pene
trometer. The penetrometer, which is a modified Allo-
Kramer Shear press, contains five needles to give resist
ance to make a time-force curve in penetrating. It was
developed by Hinnergardt (42) to use in comparing sensory
scores when cooked pork chops were evaluated. The results
of his study showed that the correlation between raw and
cooked pentration was 0.6982, the correlation between raw
penetration and the sensory panel was 0.5032, and between
cooked penetration and the sensory panel was 0.6283. The
correlations were statistically significant and its advan
tage was due to the fact that it was nondestructive compared
15
to other devices.
Kulwich et. al . (47) developed and evaluated a slice-
tenderness evaluator (STE). The STE consists of a sample
holder in which the slice of cooked meat is mounted, and a
penetrator first punctures, and then shears off a circular
portion of the slice. This STE was used in conjunction
with a commercial materials-testing instrument that pro
vided for continuous recording of the force-penetration
curve. The results showed that STE shear force was a more
effective predictor of taste panel tenderness score than
STE puncture force. The simple correlation coefficient
between STE shear force and taste panel tenderness score
was -0.72, and the correlation between STE puncture force
and taste panel tenderness score was -0.65. The advantage
of the STE technique is that the measurement is made on a
0.2 inch-thick slice, which is typical of a roast meat
serving. It can also measure at many locations in a slice.
Sperring et. al . (46) devised a tenderness press for
measuring meat tenderness by pressure. In this technique,
a meat sample was compressed by a motor driven hydraulic
press between a plunger and a cylinder base. The reading
on the pressure gauge, when the first protrusion of sample
muscle appeared in the hole of the disk was taken as the
toughness of that sample. When the tenderness press was
used to measure tenderness of beef samples it showed a
significant correlation with Warner-Bratzler shear values
16
and a highly significant correlation with the sensory panel.
Alsmeyer eit al . (48) developed a modified tenderness press
and v^en compared with the Warner-Bratzler shear values,
the STE method and sensory scores, results indicated that
all the methods were equally effective as measures of beef
tenderness. When correlated with the panel scores from pork
samples with high marbling the scores were -0.88, -0.90 and
-0.89 respectively.
A more recent device for measurement of tenderness
in raw meat is known as the Armour Tenderometer (49) . This
apparatus consists of a probe assembly and a strain gauge.
The probe assembly includes 10 stainless steel needles,
each 3 inches long, mounted on a manifold attached to a
strain gauge, which is connected by cable to a peak force
indicator. The depth of the needle penetration is deter
mined by a guard bar that regulates the penetration to
exactly 2 inches. The instrument was designed to be used
on the raw longissimus dorsi at the area of the 12th and
13th thoracic vertebrae while the carcass hangs from a
cooler rail. Readings should be taken on muscle chilled
to a minimum of (4°C) but not more than (0 C).
Henrickson et. al.. compared tenderometer values with
Warner-Bratzler shear scores and found them unrelated (11).
The authors felt that the units most likely measured a dif
ferent element of tenderness. The tenderometer measures
the force necessary to separate the individual raw muscle
17
fibers, while the Warner-Bratzler shear measures the force
required to cut the cooked fibers at right angles to their
long axis. However, Luckett et_ al . (50) compared the same
two devices using rib steaks from 104 steer carcasses. A
highly significant correlation coefficient was obtained
between tenderometer and Warner-Bratzler shear values
(r = 0.48). Tenderometer values were also significantly
(P<.01) associated with carcass weight (r = -.32), yield
grade (r = -.18) and marbling score (r = 0.00). Based on
this data, the tenderometer when compared with the Warner-
Bratzler shear, was of value in measuring carcass tender
ness.
CHAPTER III
METHODOLOGY
Animals
Eighty-eight crossbred pigs consisting of 43 gilts
and 45 barrows were purchased from a commercial producer.
All the animals had been reared in complete confinement.
In order to determine sex differences as well as the
effect of ration, corn, milo and wheat were compared by feed
ing each treatment to 15 barrows, corn and milo to 14 gilts
and wheat to 15 gilts. The pigs were started on a grain-
soybean meal ration containing approximately 17% protein and
changed to a 14% protein ration when they weighed about 135
pounds. The animals were removed from the experiment when
they exceeded 215 pounds on the weekly weighing day, and
slaughtered at a.local packing plant the following morning.
Ration
Corn and grain sorghum used in the rations were pur
chased from local sources. The grain sorghum was locally
produced, elevator-run type. The corn was trucked into the
area and the exact origin was not known. Wheat was produced
under irrigation on the Texas Tech University Farm. It was
a hard, red winter wheat, Wichita variety. A quantity of
each grain and soybean meal adequate to complete the study
was stored and analyzed for lysine content prior to formu
lating the rations.
18
19
Marbling
Degree of marbling of the pork carcasses was deter
mined by using a 10-point USDA Scale ranging from devoid-1
to abundant-10. Three categories were used for classifying
the pork: 1) traces or slight degree of marbling with a
score of 3 to 4; 2) small, modest or moderate amounts of
marbling, 5, 6, or 7; 3) slightly to moderately abundant
with a score ranging from 8 to 9.
The Sample
The sample consisted of 88 roasts taken from the
loin of the right side of each animal. Each roast was re
moved beginning 11 centimeters posterior to the fifth rib.
The roasts were wrapped, labeled, blast frozen, and main
tained at -23.3 C until ready for cooking.
Cooking Procedure
Six roasts from one ration, with three marbling
levels from each sex, were prepared at each test period.
The cooking periods were every Tuesday and Thursday for
seven weeks until 88 roasts were tested.
Thirty-six hours before cooking, the six roasts for
one test period were removed from the freezer and allowed
to thaw in a refrigerator at 6°C. Each roast was then un
wrapped, coded, and weighed. Three readings for tenderness
were made on each roast with the Armour Tenderometer by the
same person each time. Each roast was placed with ribs down
20
and anterior end to the front in a Teflon-coated pan. A com
mercial double deck Toastmaster electric oven was preheated
to 177 C before placing the roasts inside to begin the cook
ing period. The internal temperature of each roast was re
corded by means of a stainless steel potentiometer lead at
tached to a Honeywell Electronik 15 Multipoint Recorder. As
soon as the internal temperature reached 77°C the roasts were
removed from the oven and allowed to cool for 15 minutes.
They were then weighed and tenderness readings were again
made with the Armour Tenderometer.
The weight difference of raw and cooked roasts was
calculated to be the evaporative loss. Aqueous and fat
drippings were weighed and poured into a graduated cylinder
and allowed to cool to determine the volume of each portion
(Appendix A).
After the cooked roasts were weighed, the bones, the
outer fat and the browned portion were removed with an elec
tric slicing knife. The trimmed longissimus muscle was then
placed on a Hobart electric slicer with anterior end down and
10 one-fourth inch thick slices were removed. A one inch
circle was taken from each slice for sensory evaluation
(Figure 1).
Sensory Evaluation
A sensory panel of 9 members was chosen to evaluate
the pork samples for appearance, odor, flavor, juiciness.
21
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22
tenderness and over-all acceptability. The sensory panel
consisted of 5 men and 4 women, all Texas Tech students.
All the members had previous experience in sensory evalu
ation.
A sheet of instructions which indicated the method
for scoring was given prior to evaluation (Appendix B). The
9 point hedonic scale ranging from 9, like extremely, to 1,
dislike extremely, was used for evaluation (Appendix C). An
additional space on the scoring sheet was designed to write
comments.
All samples were color coded and served on white
plates. Caution was exercised so that each judge would re
ceive the same numbered slice from each roast each time.
The plate of samples and a glass of slightly acidulated
water for rinsing the mouth between samples were placed in
individual booths.
Warner-Bratzler Shear
For use with the Warner-Bratzler Shear Apparatus
three, one-half inch in diameter cores were removed by the
hand method (52) from the posterior portion which remained.
Three shear readings were taken on each core and the values
were averaged.
Total Fat Content
The lean muscles which were left after core samples
were removed for the Warner-Bratzler shear values were then
23
placed in an Oster electric blender to obtain a homogenous
mass. A 10 gram sample was weighed from each homogenized
mass for the fat analysis.
A simplified Folch et. aj^. method (52) developed by
Bligh and Dyer (53) was used to determine fat content. The
method has been compared with the official AOAC method,
ether extraction, alkaline saponification method, and the
Mojonnier method and was found to give more desirable result
than others (54).
CHAPTER IV
FINDINGS AND INTERPRETATION
Data were analyzed to determine variations caused by
treatment, marbling levels, and sex. Differences in tender
ness as evaluated by Armour Tenderometer, Warner-Bratzler
shear and sensory panel were determined by analysis of vari
ance. When the difference was significant, the Duncan's
Multiple rank order was applied to determine the order of
difference.
For analysis of data obtained from sensory panel
evaluation the standard deviation, standard errors, and the
significance of difference between means were calculated
and analyzed by analysis of variance (55).
Variation Caused by Treatment
Variation in Cooking Losses
There were no significant differences (P>.05) noted
in cooking losses of roasts from animals fed three different
types of grain rations. The roasts from animals fed the
corn rations appeared to have lower cooking losses than those
from other treatments, but the difference was statistically
non-significant (P> .05). Moreland observed that pork from
the wheau treatment had the least weight loss as a result of
cooking (6), however, the result was also statistically non
significant. No interaction was observed between grains and
24
25
marbling, or grains and sex. It appears that the variations
in cooking losses were probably caused by individual animal
differences rather than by treatment (32).
Sensory Panel Evaluation
Samples of roasts from different treatments were
rated from 9 (like extremely) to 1 (dislike extremely) by
sensory panel members.
Sensory evaluations for appearance, odor, flavor,
juiciness, tenderness and overall acceptability were ana
lyzed, and showed no statistically significant differences
attributable to the three rations, or from interactions
between grain and sex, or grain and marbling. However, a
slight tendency indicated that roasts from animals fed the
grain sorghum ration were less tender (lower tenderness
score, higher chew count number) than the roasts from ani
mals fed the wheat or corn rations. When Moreland (6) con
ducted her investigation on effects of four rations, corn,
wheat and sorghum on pork quality, she found that pork from
the grain sorghum treatment was less tender than that from
the other treatments (P<.05) when evaluated by the sensory
panel. However, for appearance, odor, flavor and juiciness
evaluation no significant differences were found which were
attributable to treatment (P>.05).
Objective Measures of Tenderness
When tenderness was evaluated by the Armour Ten
derometer in either raw or cooked pork no significant
26
differences were noted which were attributable to ration
(P>.05). Neither was a difference found as a result of
interactions between grains and marbling or grains and sex
(P>.05). The Warner-Bratzler shear values of cooked meat
also showed no significant difference in tenderness due to
treatment (P>.05). However, a slight trend was apparent
indicating that cooked roasts from the grain sorghum treat
ment were slightly less tender than roasts from the other
treatments (Table 1). These findings were similar to those
noted by Moreland (6).
Intramuscular Fat
Least-square analysis of variance showed intramus
cular fat was significantly affected by different grain
treatments (P<^.01). Duncan's multiple rank order test
showed no significant differences in intramuscular fat
content of roasts from corn and grain sorghum treatments
(P>.01). However, the roasts from pigs receiving the wheat
ration had a significantly higher fat content than the corn
and grain sorghum fed pigs (P <.01).
Variation Caused by Marbling
Least-square means for selected variables for the
three marbling levels are shown in Table 2. Analysis of
variance was used to determine the differences. When the
differences were significant, a Duncan's multiple rank
order test was applied.
TABLE 1
LEAST SQUARE MEANS FOR VARIABLES DUE TO TREATMENT
27
Corn Grain Sorghum Wheat
Raw weight (gms)
Cooked weight (gms)
Evaporative loss (% of raw wt) 19.15
Total drip loss (% of raw wt)
Fat loss (% of total drip)
Water loss {% of total drip)
Intramuscular fat {%)
1190 .82
9 6 2 . 8 4
1 9 . 1 5
4 . 5 3
6 3 . 3 7
3 7 . 2 7
1 1 8 4 . 9 1
941-40
2 0 . 5 3
5-40
6 5 . 7 8
3 4 . 2 1
1149 .20
9 1 0 . 1 0
20 .77
4 . 7 1
7 3 . 2 5
2 6 . 7 5
5.88 5.60' 7.38'
Tenderometer (raw) (lbs)
Tenderometer (cooked) (lbs)
Shear values (lbs)
Appearance
Odor
Flavor
Juiciness
Tenderness
Number of chew counts
Overall acceptability
6 .96
1 6 . 4 8
6 . 7 2
6 . 7 9
6 . 8 7
6 . 7 1
6 . 6 2
7 .10
2 3 . 1 3
6 . 8 2
7 . 0 5
17 .09
7 .68
6 . 6 3
6 . 6 4
6 . 7 0
6 . 4 5
6 . 7 5
2 4 . 3 8
6 . 5 8
7 .52
16 .00
7 .36
6 . 5 9
6 . 7 5
6 . 7 5
6 .40
7 .22
22 .97
6 . 7 4
'•• Means for a factor bearing different superscripts are significantly different P<.01.
*Sensory evaluations based on Fledonic Scale (1-9) .
TABLE 2
LEAST SQUARE MEANS FOR VARIABLES DUE TO MARBLING
28
Low Moderate High
Raw weight (gms)
Cooked weight (gms)
Evaporative loss (% of raw wt) 21.17
Total drip loss (% of raw wt)
Fat loss (% of total drip)
Water loss (% of total drip)
Intramuscular fat (%
173.13
925.37
21.17
4.88^
66.18
33.82
1177.67
944.23
19.82
5.36^
65.61
35.59
1174.13
944.74
19.51
4.39
70.90
29.12
Tenderometer (raw) (lbs)
Tenderometer (cooked) (lbs)
Shear value (lbs)
* Appearance
Odor
Flavor
Juiciness
Tenderness
Number of chew counts
Overall acceptability
5.64"-
7.92
17.48^
7.98*
6.63
6.64
6.45^
6.20-
6.68''
25.34^
6.40
6.39"
6.93
16.86^
7.13^
6.71
6.71
6.80'
6.53
7.03
23.68
6.83'
6.83
6.68
15.24^
6,64^
6.67
6.67
6.91^
6.74^
7.35^
21.46^
6.91^
a^^Means for a factor bearing different superscripts are significantly different P<.01.
^'^Means for a factor bearing different superscripts are significantly different P<.05. Means without a superscript are not significantly different from each other. *Sensory evaluations based on Hedonic Scale (1-9).
29
Variation in Cooking Losses
No significant difference was found in evaporative
loss due to marbling levels, interaction between marbling
and sex or marbling and grain (P> .05) . However, the total
drip loss from the moderate marbling level was significantly
higher (P<.01) than from either low or high marbling level.
The composition of the drip was not affected by marbling
(P> .05).
Sensory Panel Evaluation
The subjective evaluation of pork indicated that the
abundantly marbled pork received higher scores in flavor,
juiciness, tenderness, and overall acceptability than the
poorly marbled roasts. The difference was statistically
significant (P<.01). When comparing the moderately marbled
roasts with the poorly marbled ones, only flavor and overall
acceptability scores were affected (P<.01). Statistically,
there were no significant differences between moderate and
high marbling in flavor, juiciness, tenderness and overall
acceptability (P> .05). However, a definite trend was
noticeable in the scores from the poorly marbled to the
abundantly marbled roasts (Table 2) . Judge ejt al . (38) also
attempted to find the relations between marbling and sensory
tenderness, juiciness and flavor. Their results showed that
only juiciness was correlated with marbling (P <.01) .
When the number of chew counts was compared, the
poorly marbled meat required more chewing than the highly
30
marbled (P<.01). Statistically, there were no significant
differences between low and moderate, and moderate and high
marbling in chew count numbers (P>.05).
Work conducted by Field et al. (37) with beef also
indicated that bull roasts with more abundant marbling re
ceived significantly higher sensory ratings when age was
held constant. Sensory ratings in the study included ten
derness, juiciness and flavor.
Objective Measures of Tenderness
The tenderometer readings on the raw pork were not
significantly affected by either marbling level or inter
action between grains and marbling, or marbling and sex
(P> .05) .
Meat tenderness measured in the cooked state by both
the Armour Tenderometer and the Warner-Bratzler shear appa
ratus showed significant differences (P<^.01) with levels
of marbling. It appeared that the higher the marbling level
the more tender the cooked meat (lower tenderometer and shear
values). These results coincide with those of Wellington's
study on beef tenderness (43). The poorly marbled pork
was significantly less tender than the highly marbled pork
(P<.01). The moderately marbled pork was more tender than
that with low marbling according to the shear values but
less tender than that with high levels of marbling when
evaluated by the tenderometer. The difference was sig
nificant (P<^.05). Field £t al_. (37) when testing beef
31
tenderness indicated that Warner-Bratzler shear scores were
not affected significantly by marbling when age was held
constant.
Intramuscular Fat
The intramuscular fat content of roasts was related
significantly to marbling (P<.01). The lower the level of
marbling, the less the intramuscular fat content of the lean
roast. Though statistically, the fat content in the high
level of marbling was not significantly higher than that in
the moderate level but the difference exists. However, the
pork with the moderate level of marbling was significantly
higher in fat content when analyzed (P<.01) than that with
the low level of marbling.
Judge et_ al . (38) found the relationship between the
degree of marbling in the muscle and the chemical fat con
tent was 0.76. They indicated that the degree of marbling
in pork as judged subjectively, is a measure of the actual
fat content of the muscle.
Variation Caused by Sex
Least-square analysis of variance showed that sex
affected significantly the total roast weight (P<.01).
Roasts from gilts were much heavier than those from barrows.
Variation in Cooking Losses
There was no significant difference in evaporative
loss due to sex difference (P>.05). However, the roasts
32
from the barrows had more drip loss than those from gilts
as a result of cooking (P<.01).
Sensory Panel Evaluation
Analysis of appearance, odor, flavor, juiciness and
tenderness scores of pork when rated by the sensory panel
showed no significant differences (P>.05) attributable to
sex or interaction between sex and grain or sex and marbling
The results are presented in Table 3. Similar results were
indicated by Skelley and Handlin (34) and Field (37).
Analysis of overall acceptability scores showed
roasts from barrows rated higher than those from gilts
(P <.05) .
Objective Measures of Tenderness
Tenderness as measured by the Armour Tenderometer
in either raw or cooked muscle and by the Warner-Bratzler
shear apparatus showed no significant differences between
gilts and barrows (P>.05). This is in agreement with the
results of Skelley (34) and Field et_ aJ . (37) who concluded
that shear value is not affected by sex difference. How
ever, Wallace (33) found that chops from barrows were more
tender than those from gilts.
Intramus":ular Fat
The intramuscular fat content of roasts was not sig
nificantly influenced by sex differences (P>.05). This
result is different from the results of Hiner et aj . (44)
33
TABLE 3
LEAST SQUARE MEANS FOR VARIABLES DUE TO SEX
Barrow Gilt
Raw weight (gms)
Cooked weight (gms)
Evaporative loss (% of raw wt)
Total drip loss (% of raw wt)
Fat loss (% of total drip)
Water loss (% of total drip)
Intramuscular fat (%)
Tenderometer (raw) (lbs)
Tenderometer (cooked) (lbs)
Shear value (lbs)
Appearance
Odor
Flavor
Juiciness
Tenderness
Number of chew counts
Overall acceptability
1145.84=*
914.76^
20.11
5.38^
72.00^
28.00^
6.50
6.75
16.42
7.25
6.63
6.75
6.78
6.60
7.09
22.85
6.85*
1204.ir
961.47
20.19
4.37'
63.12
3 7 .
6 .
7 .
1 6 .
7.
6.
6.
6,
6
6
24
69"
07
,60
,63
.25
. 71
.76
. 6 5
. 3 8
. 9 6
-14
d
6.58
^'^Means for a factor bearing different superscripts are significantly different p<.01.
^'^Means for a factor bearing different superscripts are significantly different P<.05.
*Sensory evaluations based on Hedonic Scale (1-9).
34
and Kauffman (41) which indicated that barrows had signifi
cantly more intramuscular fat in the longissimus muscle than
gilts.
Correlation Among Tenderometer, Warner-Bratzler Shear, Sensory Tenderness Scores and Number of Chew-Counts
Correlations among measurements of tenderness by
different methods are presented in Table 4. Tenderness of
cooked pork measured by the Armour Tenderometer was signifi
cantly correlated (P<^.01) with the Warner-Bratzler Shear
Apparatus (r = .59), the sensory tenderness score (r = -0.47),
and the number of chew counts (r = .47) . However, the ten
derometer values of the raw pork were not related to shear
values nor to sensory tenderness values. The tenderometer
values from the raw and the cooked pork were significantly
related, however, (P<.05) (r = .26). The Warner-Bratzler
shear values were found to be related to every tenderness
measurement except the tenderometer value for the raw pork
(P>.05). The sensory panel evaluations of pork tenderness
for both tenderness scores and chew count numbers were sig
nificantly related to tenderometer measurements on the cooked
pork and to Warner-Bratzler shear values (P<.01). Similar
results were found by Field ejt aj . (37) .
In sensory evaluations for tenderness the higher the
score the more tender the meat, which is in contrast with
other measurements since the higher the reading the tougher
the meat. Therefore, the sensory tenderness scores were
35
negatively correlated with the other measurements.
TABLE 4
CORRELATION COEFFICIENTS BETWEEN TENDEROMETER, WARNER-BRATZLER SHEAR, SENSORY TENDERNESS
AND CHEW-COUNT SCORES
1. Raw tenderometer 1.00
2. Cooked " 0.26* 1.00
3. W-B shear 0.03 0.59** 1.00
4. Sensory tender 0.04 -0.47** -0.70** 1.00
5. No. chew count 0.02 0.47** 0.73** -0.88** 1.00 •
*P <.05 r = .232
**P< .01 r = .302
Selected Variables
r I'l i M
Correlation Coefficients Between * n
Simple correlation coefficients were analyzed to j
determine the relations among the following selected vari
ables: intramuscular fat, raw pork tenderometer readings,
cooked pork tenderometer readings, Warner-Bratzler shear
values, sensory scores of appearance, odor, flavor, juici
ness, tenderness, number of chew counts, and overall ac
ceptability. The results are presented in Table 5.
It appears that flavor, juiciness, and overall ac
ceptability were highly correlated with every selected
variable except intramuscular fat and tenderometer readings
measured in raw pork (P<.01).
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37
The intramuscular fat content was related to raw
pork tenderometer measurements at 1% level (r = .31), but
was nonrelated to any other variables.
The results of correlations between juiciness,
flavor and overall acceptability supports the findings of
Pengilly and Thornton (40) who concluded that flavor and
juiciness were correlated with overall acceptability.
Results also are compatible with the study conducted by
Judge e;t a^. (38) , and Field et_ al_, (37) that tenderness,
juiciness, and flavor were all correlated.
CHAPTER V
SUMMARY AND CONCLUSIONS
Texas is the leading grain sorghum producing area in
the United States since it produced in 1972 about half of the
nation's total. Due to climatic conditions, corn is not
grown in as great a quantity as grain sorghum in Texas.
Therefore, the substitution of grain sorghum for corn in
swine ration would be not only convenient but also economi
cal and agriculturally sound.
This study was undertaken to determine the differ
ences in pork quality which could be attributed to three
rations, wheat, corn and grain sorghum, and the interaction
effects of sex differences and of three levels of marbling.
In order to make the comparison, 88 loin roasts from
43 gilts and 45 barrows were used. Roasts were selected
from 15 barrows fed each ration and from 14 gilts fed corn
and sorghum and 15 gilts fed wheat ration. Three marbling
levels within each treatment were determined according to
the USDA Scale.
In determining the pork tenderness, the Warner-
Bratzler Shear Machine and the Armour Tenderometer were the
objective methods used. For subjective methods, a 9 member
sensory panel evaluated the pork for tenderness by determin
ing the ease of chewing and the number of chew counts re
quired to comminute the pork sample to a mushy consistency.
38
39
Other sensory qualities such as juiciness, flavor, and over
all acceptability were evaluated to determine their relation
to each other and to tenderness.
Results of the study indicated that variation of
grains in the ration did not have a significant effect on
cooking losses, meat tenderness, flavor, juiciness, odor
and appearance. However, slight trend indicated that roasts
from grain sorghum fed animals were less tender than those
roasts from the wheat or corn fed animals. The trend was
observed through lower tenderness scores, higher chew count
number, higher shear values, and higher tenderometer read
ings measured on cooked roasts. The intramuscular fat con
tent was significantly affected by the various grain treat
ments. The roasts from animals fed the wheat ration had a
higher fat content than the roasts from animals fed the corn
or grain sorghum rations.
Marbling level significantly affected the intra
muscular fat content, tenderness of cooked pork, and the
flavor, juiciness, and overall acceptability. The more
abundantly marbled pork received the better rating in qual
ity. Even though the consumer is sensitive to fat in meat,
it must be concluded that the higher levels of marbling
resulted in roasts which were not only more tender by all
methods of evaluation but also were scored higher for flavor,
juiciness and overall acceptability.
40
Sex difference affected the total roast weight as
roasts from gilts were heavier than those from barrows.
Other quality factors such as intramuscular fat content,
tenderness scores and scores for appearance, odor, flavor
and juiciness were not significantly affected by sex dif
ference. However, roasts from barrows received signifi
cantly higher overall acceptability scores than those from
gilts.
When comparing different methods for evaluating pork
tenderness, both the Warner-Bratzler Shear and the Armour
Tenderometer values for the cooked pork were as precise in
determining tenderness as sensory evaluations. When the
Armour Tenderometer was used to measure tenderness of raw
pork it correlated only with those values from cooked pork
at 5% level of significance, but the raw pork values were
not significantly correlated with values from other methods.
Among selected variables, flavor, juiciness, and
overall acceptability were highly correlated with every
selected variable except intramuscular fat and tenderometer
readings measured in raw pork.
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45
46. Sperring, D.D., W. T. Piatt, and R. L. Hiner. Tenderness m beef muscle as measured by pressure. Food Tech. 13: 155, 1959.
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48. Alsmeyer, R, H. , J, W. Thornton, R. L. Hiner, and N. C. Bollinger. Beef and pork tenderness measured by the press, Warner-Bratzler, and STE method. Food Tech. 20: 683, 1966.
49. Hansen, L. J. Measuring the tenderness of meat. U. S. Patent3, 602, 038. Armour and Company, Chicago, Illinois, 1971.
50. Luckett, R. L., T. D. Bidner, B. E. Humes, E. A. Icaza. Comparison of tenderometer and Warner-Bratzler Shear. Food Science and Technology Section Abstracts. 70th meeting Association of Southern Agricultural Workers, Atlanta, Georgia, February 4-7, 1973.
51. Kastner, C. L., and R. L. Henrickson. Providing uniform meat cores for mechanical shear force measurement. J. Food Sci. 34: 603, 1969.
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53. Bligh, E. G., and W. J. Dyer. A rapid method of total lipid extraction and purification. Can. J. Biochem, Physiol. 37: 911, 1959.
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APPENDIX
A. Pork Roast Marbling Study Data Sheet
B. Instructions to Sensory Panel Members
C. Sensory Panel Score Sheet
46
47
APPENDIX A
PORK ROAST MARBLING STUDY DATA SHEET
RATION SEX
MARBLING GRADE
ANIMAL NUMBER
SYMBOLS
1. Weight of pan and raw roast
2.
3.
4.
5.
Cooking time
Weight of pan (gms)
Difference (wt of roast)
Weight of pan & cooked roast
6. Weight of pan & drippings
7. Weight of cooked roast
8. Weight of drippings (3-6)
9. Evaporative loss (4-7)
10. Total volume (ml) drippings
11. Volume of fat portion
12. Volume of aqueous portion
13. Tenderometer readings: raw
cooked
average
14. Shear values: core 1
core 2
core 3
average of all cores
48
APPENDIX B
INSTRUCTIONS TO SENSORY PANEL MEMBERS
HOW TO SCORE
•nnnMn^of ^ ^T""^ for appearance, odor, flavor of lean, juiciness, and overall acceptability within a range of 9
^ L ;^^^^^ T^^^^S'"''^ ^^^^^ ^ ° ^ descriptive terms. Record the score which best describes your impression of the sample. /
SCORING FOR APPEARANCE
Large portions of the meat will be available for scoring appearance. ^
SCORING FOR ODOR
A small snifter containing warm chopped meat and covered by a watch glass will be available for scoring odor.
SCORING FOR FLAVOR AND JUICINESS
Bite the 1 inch circle of meat in half and record the score describing your impression of flavor and juiciness at the beginning of the chewing period.
SCORING FOR TENDERNESS
Count the number of times you chew the half circle of meat before swallowing it. Record the number of chews required to thoroughly masticate the sample to a mushy consistency. Record a score from 9 to 1 that describes your impression of the tenderness of the half circle.
Set up for yourself a range of the number of chews for each score from 9 to 1. For example: if you chew from 5 to 15 times, you might record a score of 9; if you chew 15-25, a score of 8; 25-35 times, a score of 7; continue to reduce the score by a given number of increased chews. Each judge should set his own range of chews for a given score.
COMMENTS
Comments about a sample and/or explaining your reason for giving a particular score are helpful and you are strongly urged to comment on each sample.
Take your time to score each sample. Water is provided for rinsing your mouth between samples.
49
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