Embryology and ES Embryology and ES CellsCells

Important to Pharmacology?Important to Pharmacology?

Are these mice REAL!!??Are these mice REAL!!??

TopicsTopics Basic Embryological DevelopmentBasic Embryological Development Collection Of ES CellsCollection Of ES Cells Using ES CellsUsing ES Cells KO/KIKO/KI ChimerasChimeras

DevelopmentDevelopment Development starts with fertilizationDevelopment starts with fertilization Then we have the cell stagesThen we have the cell stages 2, 4, 8, 16, 322, 4, 8, 16, 32 Stage 32 is often called morulaStage 32 is often called morula Blastocyst (Important!!!!)Blastocyst (Important!!!!) GastrulaGastrula Let’s stop thereLet’s stop there

Diagram of early developmentDiagram of early development http://www.molbio.http://www.molbio.

princeton.edu/courprinceton.edu/courses/mb427/2001/prses/mb427/2001/projects/09/SCtutorialojects/09/SCtutorial.htm.htm

Blastocysts to ???Blastocysts to ???

ES CellsES Cells What are Embryonic Stem Cells (ES What are Embryonic Stem Cells (ES

cells)cells) National Institute of Health describes National Institute of Health describes

them as cells that capable of dividing them as cells that capable of dividing for long periods of time, unspecialized for long periods of time, unspecialized and able to become any other type of and able to become any other type of cellcell

How can these be useful in How can these be useful in Pharmacology?Pharmacology?

UsageUsage Genetic Genetic

manipulations manipulations Drug testingDrug testing Ability to study Ability to study

cellular cellular developmentdevelopment

Engineer organs?Engineer organs? We just do not We just do not

know all the know all the informationinformation

SO how do we make green mice?SO how do we make green mice?

How do we express altered ES How do we express altered ES cells in neonates?cells in neonates?

So where do the Green Mice come So where do the Green Mice come in?in?

So why do we want to understand So why do we want to understand this procedure?this procedure?

How can it be used for How can it be used for pharmacology?pharmacology?

What could that mean to “ME?”What could that mean to “ME?”