endocrine histology lab - كلية الطب · thyroid gland the thyroid gland consists almost...

Endocrine histology lab

Dr.Deema Hamarsheh

Lama

Pituitary gland ( Hypophysis) Adrenal gland Thyroid gland Parathyroid gland Pineal gland Islets of langerhans

The hypophysis consists of two glands :

Adenohypophysis

Neurohypophysis

Pars distalis ( anterior pituitary)

Pars tuberalis

Pars intermedia

Pars nervosa

Infundibulum ( neural stalk )

Stem

Median eminence

( oral ectoderm )

( nerve tissue )

â•‘iPad â•‘Lama

Paras distalis subdivided into A)acidophil B)basophil C)chromophobes



Chromophobes are less numerous and represent undifferentiated parenchymal cell or degranulated cells


Consist of modified neural tissue Containing unmyelinated axons Supported by glia cell called pituicytes


Contains cysts reminant of rathkes pounch


Chromophobes are less numerous and represent undifferentiated parenchymal cell or degranulated cellsUnstained

We have three cell types based on their differential staining with H&E :

1) Basophils 2) Acidophils 3) Chromophobes (unstained or

weakly stained cells )





Bluish


oacidophilic structures red or pinkThis is also sometimes termed 'eosinophilicThus the cytoplasm is stained pink in the picture below, by H&E staining.



Acidophiluc ....orange


Basophilic ....granules



Acidicophilic cells



Basophilic cells


FSH and LH work together in the reproductive system.(FSH)....FSH regulates the development, growth, pubertal maturation and reproductive processes of the human body.In both males and females, FSH stimulates the maturation of germ cells(lH)....In females: ovulation.In males: testosterone secretion.

The neurohypophysis consists of : 1) unmyelinated nerve fibres derived

from neurosecretory cells of the supraoptic and paraventricular hypothalamic nuclei

2) Pituicytes (oval or round nuclei ) - 25% of the volume - specific type of glial cells







The axons run from the supraventicular and supraoptic in hypothalamic nuclei and have swellings called neurosecretory Herring bodies From either oxytocin and ADH is released upon neural stimulation


Neurosecretory material consists of two hormones : 1) Oxytocin from mostly from paravetricular nuclei

2) antidiuretic hormone (ADH or vasopressin ) mostly from

supraoptic nuclei







Adrenal Gland

The adrenal (or suprarenal) glands consist of : an outer cortex (the main part of the adrenal

glands)

inner medulla


cortex

he cortex is divided into three concentric zones which, from the surface inwards, are termed : The zona glomerulosa (accounting for about 15% of the cortical

thickness) ----mineralocorticoids are produced in this zone The zona fasciculata (about 75%) ------glucocorticoids The zona reticularis(about 10%) ------ sex hormones










Ovoid structural


Spongy like cellLipid droplets


Pale basophilic


Highly basophilic


As a result this staining technique can reveal collagen

Medulla

Cells are arranged in strands or small clusters. The cytoplasm of the cells is weakly basophilic. They are called chromaffin cells because the

granules of these cells can be stained with potassium bichromate.

Secretes catecholamine derived from neural crest cells.


Epinephrine more abundant


Pale stain cell are the chromoffin cell

Thyroid Gland

The thyroid gland consists almost entirely of rounded cysts, follicles, which are separated by scant interfollicular connective tissue.

The colloid is the secretory product of the follicular cell , Its main component, thyroglobulin, consists of triiodothyronine and tetraiodothyronine (or thyroxine).

C cells (or parafollicular cells) surrounds the entire follicle.







Follicular cell range in shape from squmous to low columnar The size and cellular features of thyroid follicles vary with their functional activity


Stain less intensely (pale) They have smaller amount of rough ER and golgi complexes They found inside the basal lamina of follicular epithelium between follicles Parafollicular cell derived from neural crest These cell synthesis and secrete calcitonin


Rest on basal lamina They are rich in rough ER and golgi complex Dark stain

Parathyroid gland

These glands are small A considerable number of fat cells infiltrate the gland

(beginning around puberty) and may account for about half the weight of the parathyroid glands in adults.



The unique character of parathyroid >>>have adipocytes

Two cell types can be distinguished in the parathyroid glands: Chief cells are the most numerous type. They are rather

small, a round, light and centrally placed nucleus and a very weakly acidophilic cytoplasm. They synthesise parathyroid hormone (PTH or parathormone) which is of pivotal importance for normal calcium concentrations in the fluids and tissues of the body.

Oxyphilic cells are less frequent (entirely lacking in small

children ,Their cytoplasm is strongly acidophilic, the nucleus is small and uniformly intense basophilic. They contain large amounts of mitochondria.



Acidophilus non functional cell Nucleus paleLesser frequency Larger

Smaller ..Darker and round center nucleus Densely packed cord of small cell

Pineal Gland

The pineal body is surrounded by pia mater

n the pineal we find two cell types: pinealocytes and astrocytes

pineal gland also contains brain sand (corpora arenacea). For calcium and magnesium

Small dark nuclei belong to the astrocytes found in the pineal gland.

Pinealocytes have larger, lighter and round nuclei, which are surrounded by a broad rim of light cytoplasm.

The most prominent secretory product of the pineal body is melatonin

Most nuclei present are the

nuclei of pinealocytes

Islands of Langerhans, usually containing several hundred endocrine cells, are scattered throughout the exocrine tissue of the pancreas.

Islands of Langerhans

75% beta-cells which secrete insulin. Insulin stimulates the synthesis of glycogen, protein and fatty acids

20% alpha-cells which secrete glucagon. The effects of glucagon are generally opposite to those of insulin. (they are typically smaller than those of delta-cells

5% delta-cells which secrete somatostatin, a locally acting hormone which inhibits other endocrine cells

PP celi : secretes pancreatic polypeptide

Endocrine paler than exocrine

endocrine histology lab - كلية الطب · thyroid gland the thyroid gland consists almost...

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