electrophoresis - bioworld · electrophoresis 68 buffers & reagents for protocols visit...

DNA / RNA

Electrophoresis

General Use

Hybridization

Immunologicals

Protein Purification

buffers&reagents

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accelerate: lifeSciencesresearch> DNase, RNase & Protease-free buffers

for protocols visit www.bio-WORLD.com

QBT Buffer - for DNA purification

#700870 250ml $ 24.50

#700871 500ml $ 39.70

• NaCl (750mM), pH 7.0

• MOPS (50mM)

• Ethanol (15%)

• Triton X-100 (0.15%)

QC Buffer - for DNA purification

#700909 250ml $ 22.50

#700910 500ml $ 37.50

• NaCl (1.0M), pH 7.0

• MOPS (50mM)

• Ethanol (15%)

QF Buffer - for DNA purification

#700912 250ml $ 22.50

#700913 500ml $ 37.50

• NaCl (1.25M), pH 8.5

• Tris-Cl (50mM)

• Ethanol (15%)

Sodium Acetate (3M)

#730120 100ml $ 16.59

#730122 200ml $ 22.47

• Autoclave sterilized

• Biotechnology Grade

• pH 5.2

Sodium Hydroxide with MSH - for protein and DNA

isolation from yeast

#700225 250ml $ 21.50

#700226 500ml $ 38.50

• NaOH (1.85M)

• β−Mercaptoethanol (7.5%)

STE Reagent - for molecular biology

#700918 1L $ 19.50

#700919 2L $ 35.00

• NaCl (100mM)

• Tris-HCl, pH 8.0 (10mM)

• EDTA (1mM)

Glucose / Tris / EDTA (Sterile)

#730605 500ml $ 27.51

#730606 1L $ 39.00

• Glucose (50mM)

• Tris (25mM)

• EDTA (10mM)

• pH 7.9 - 8.1

DNA Denaturing Solution - guaranteed sterile and

DNase-free

#700845 500ml $ 21.50

#730601 1L $ 37.50


• 1.5M NaCl, 0.5M NaOH

• pH (25°C) >11.5

• Storage: RT

• Hazard: Corrosive

Plasmid DNA Isolation Solution 1

Solution 3

Solution 2

#700860 250ml $ 21.55

#700861 500ml $ 34.90

• Glucose (50mM)


• EDTA, pH 8.0 (10mM)

• NaOH (0.2N)

• SDS (1%)

• Potassium (3M)

• Acetate (5M)

#700863 250ml $ 23.00

#700864 500ml $ 34.90

#700866 250ml $ 19.50

#700867 500ml $ 34.90

All 3 Solutions

#700859 250ml $ 59.70

DNase, RNase & Protease-free buffersfor protocols visit www.bio-WORLD.com

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Sterile, nuclease-free buffers & reagents have

been prepared with the finest Biotechnology

Grade chemicals in specially purified 3D water

treated to remove particulates, salts, dissolved trace

metals, organic compounds & microorganisms.

Alkaline DNA Gel Running Buffer

#700841 250ml $ 19.50

#700842 500ml $ 35.00

• Biotechnology grade

• NaOH (0.5M)

• EDTA (50mM)

SDS Urea Buffer- - for protein & DNA isolation from yeast

#700227 250ml $ 19.50

#700228 500ml $ 36.90

• SDS (5%)

• Urea (8M)


• Bromophenol Blue (0.005%)

Tris Buffered Saline - for Western blot washing

High Salt (10X)

Low Salt (10X)

#740116 1L $ 23.75

#740117 2L $ 42.75

#740118 4L $ 66.50

• pH at 25°C 7.4

• Tris (500mM)

• NaCl (5M)

• Storage: RT

• pH at 25°C 7.4

• Tris (500mM)

• NaCl (1.5M)

• Storage: RT

#740111 1L $ 19.95

#740112 2L $ 35.15

#740113 4L $ 66.50

TE (Tris / EDTA) - buffer for DNA isolation & electrophoresis

#730153 100ml $ 16.84

#730155 500ml $ 49.70

• Sterile Solution

• Tris (10mM)

• EDTA (1mM)

• pH 8.0 (+0.1)

• DNase : None

• Storage: RT

-

Agarose Gel Loading Dye 6X - for molecular biology

Glycerol with Xylene Cyanol FF

Sucrose

#700815 25ml $ 19.00

#700817 50ml $ 27.50

#700813 25ml $ 19.00

#700814 50ml $ 27.50

• No Bromophenol Blue

• Xylene Cyanol FF (0.25%)

• Glycerol (30%)

#700820 25ml $ 28.50

#700821 50ml $ 47.50

#700818 25ml $ 28.50

#700819 50ml $ 47.50

Alkaline• NaOH (300mM)

• EDTA (6mM)

• Bromocresol Green (0.25%)


• Ficoll (18%)



• Ficoll in water (15%)



• Sucrose (40% w/v)

Glycerol with Bromophenol Blue

#700811 25ml $ 19.70

#700812 50ml $ 28.50



• Glycerol (30%)

Ficoll

Yeast Lysis Solution - for DNA isolation

#700223 250ml $ 29.00

#700224 500ml $ 45.60

• LiCl (2.5M)

• Tris-HCl (50mM), pH 8.0

• Triton X-100 (1%)

• EDTA (62.5mM)

New

buffers&reagentsElectrophoresis

buffers&reagents

IPG-Strip Equilibration Buffer

#705940 50ml $ 49.50

#705955 100ml $ 86.60

• For equilibration of IPG- Strip

before running second

dimension SDS-PAGE.

• No urea-induced protein

carbamylation

• No waste of unused reagents

• Improves resolution and prevents

streaking of protein spots on 2D gels.

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MES-SDS Running Buffer (20X)

#743146 500ml $ 34.50

#743147 1L $ 59.70

• Dilute with deionized water

to make 1X concentration.

• 1X concentration: MES

(50mM), Tris base (50mM),

SDS (0.1%), EDTA (1mM)

• pH 7.3

Laemmli SDS-Sample Buffer - electrophoretic

dye for denaturation of proteinsReducing, (4X - 6X)

Non-reducing, (4X - 6X)

4X- #742124 25ml $ 19.00

#742118 50ml $ 28.50

6X- #742110 25ml $ 24.50

#742112 50ml $ 39.80

4X- #742126 50ml $ 28.50

6X- #742114 25ml $ 24.50

• 1X solution: SDS (2%), Glycerol

(10%), Tris - HCl (62.5mM),

Bromophenol Blue and

β - mercaptoethanol

• DNase and Protease free

• Stability (20°C) >1 year

• Storage: RT

• Ultra Pure Grade

• 1x solution: SDS (2%), Glycerol

(10%), Tris -HCl (62.5mM), and

Bromophenol Blue.

• DNase, RNase and

Protease free

• Stability (20°C) >1 year

• Storage: RT

Orange-G Loading Dye - for molecular biology

#700822 10ml $ 19.00

#700823 25ml $ 34.50

Blue (6X)

Glycerol (6X)

#700824 25ml $ 19.00

#700825 50ml $ 28.50

• 6X concentration

• Orange-G (0.25%)

• Glycerol in water (18%)

• Orange-G (0.25%)



• Ficoll in water (30%)

• EDTA (30mM)

Isoelectrofocusing (IEF) Buffer

Anode (50X), pH 2.4

#743140 500ml $ 16.25

#743141 1L $ 26.75

#743142 250ml $ 36.25

#743143 500ml $ 62.70

Cathode (10X), pH 10.1

#742133 25ml $ 19.00

#742134 50ml $ 28.50

• 20mM Lysine (free base) and

Glycerol (15%)



• 1X concentration: PH 2.4

Phosphoric Acid (7mM)

• Proteases free



• 1X concentration: 20mM

Arginine and 20mM Lysine

Sample Buffer (4X) - for protein electrophoresis

accelerate: electrophoresis>


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SDS Separating Gel Buffer (4X)

#700952 100ml $ 00.00

#700951 250ml $ 00.00

#700950 500ml $ 00.00

• Standard SDS polyacrylamide

gel 10% electrophoresis

separating gel buffer

• Buffer in 0.375M Tris, pH 6.8

• 20% (w/v) SDS

Tris - for protein analysis

Tricine-SDS Running Buffer (10X)

Sample Buffer (4X)

#743150 500ml $ 28.50

#743151 1L $ 48.50

#742106 25ml $ 21.00

#742108 50ml $ 31.50

• Tris-HCl 400mM, Glycerol (40%)


• pH 8.6


• 1X contains: Tris base (50mM),

Tricine (50mM), SDS (3.5mM)

pH 8.24

SDS Sample Loading Buffer

#700958 50ml $ 29.50

#700957 100ml $ 39.70

#700956 200ml $ 67.50


electrophoresis sample loading

buffer

• 0.05% (w/v) bromophenol blue

• Dilute samples to at least 1:4

with the sample buffer.

• Heat samples at 95ºC for 4 minutes prior to loading.


gel 10% electrophoresis

separating gel buffer

• Buffer in 0.375M Tris, pH 8.8

• 20% (w/v) SDS

SDS Stacking Gel Buffer (4X)

#700954 100ml $ 00.00

#700953 200ml $ 00.00

#700950 500ml $ 00.00

#730144 1L $ 39.50

#730134 4L $ 78.00

Liquid Concentrate (25X)

• 1X contains: Acetate (0.04M),

EDTA (0.001M )

• pH 8.3 + 0.1

• Storage: RT

Ready-Pack (10X)

TAE Buffer (Tris Acetate EDTA) - for electrophoresis

#730125 1x1L/pk $ 14.95

#730127 10x1L/pk $ 56.35

#730128 2x1L/pk $ 10.25

Ready-Pack (25X)• Each pack makes 1L of 25X

• 1X contains: Tris (0.04M ),

Acetate (0.04M ), EDTA

(0.001M)

• pH at 25C: 8.2 - 8.4

• (P/F): Pass

• Storage: RT

#730137 1L /pk $ 39.77

#730131 2x1L/pk $ 59.50

#730135 10x1L/pk $ 170.2

#730129 1x40L/pk $ 59.50

#730136 500ml $ 23.70

#730138 1L $ 38.40

#730140 4L $ 97.60

Liquid Concentrate (50X)

• Each Ready-Pack prepares

1L of 10X concentrate.

• pH 8.3 + 0.1

• Same as above

• Each pack makes 50X of stated

volume

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accelerate: electrophoresis>


TGS Buffer - for protein separations

Tris Glycine - SDS (10X)

#705932 1L $ 19.95

#705935 2L $ 35.15

#705943 4L $ 66.50

#730175 5x1L/ready pk $ 31.05

#705931 1x40L/ready pk $ 26.70

#730173 2x1L/ready pk $ 35.64

#705930 2x40L/ready pk $ 37.50


• 1X solution contains: Tris

(0.025M), Glycine (0.192M),

SDS (0.1%)

• pH (5X dilution of

reconstituted buffer) 8 - 8.5

• Storage: RT

TG Buffer - for electroblotting proteins in Western Blots

Liquid & Powder (10X)

#705923 1L $ 19.95

#705925 2L $ 35.15

#705926 4L $ 66.50

#730171 1x4L/pk powder $ 55.13

#730165 2x1L/pk powder $ 27.85

#730168 5x1L/pk powder $ 49.68

#705929 1x40L/pk powder $ 26.70

#705928 2x40L/pk powder $ 39.50


• 1X TG contains: Tris base

(0.025M), Glycine (0.192M)

• pH 8.3 Tris-Tricine (TT) Buffer - for nondenaturing & denaturing

electrophoresis of proteins


• pH (1X) @ 25ºC: 8.2-8.4

• Storage: RT

• Hazard: Irritant

TT (10X)

#705969 1L $ 72.80

#705970 1x1L/ready pk $ 64.70

#705973 2x1L/ready pk $ 98.50

Tricine-SDS Sample Buffer - for protein analysis Non-Reducing (2X)

Reducing (2X)

#742102 25ml $ 19.00

#742104 50ml $ 28.50

#742148 25ml $ 19.00

#742150 50ml $ 28.50

• Contains Tris -HCl, SDS (8%),

Glycerol (20%), Coomassie Blue

G (0.01%), pH 8.45

• Contains Tris -HC (450mM),

SDS (4%), Glycerol (10%),

Coomassie Blue G (0.0025%),

β-mercaptoethanol (2%), pH 8.45

Tris-Borate Buffer

Tris-Borate EDTA Sample (4X-6X) - for nucleic acid analysis

Tris-Borate Urea Sample Buffer (2X) - for protein analysis

4X- #700944 25ml $ 15.00

#700945 50ml $ 25.00

6X- #742144 25ml $ 19.00

#742146 50ml $ 28.50

#742152 25ml $ 28.50

#742154 50ml $ 47.50

• Tris base (270mM)

• Boric Acid (270mM)

• Glycerol (36%)

• Bromophenol Blue G (0.025%)

• Xylene Cyanol (0.025%)

• EDTA (6mM)

• Tris base (45mM)

• Boric Acid (45mM)

• Urea (3.5m)

• Bromophenol 0.005%

• Xylene Cyanol 0.025%

• EDTA (1mM)

Urea Running Buffer (10X)

#700947 1L $ 16.00

#700948 2L $ 28.00

#700949 4L $ 50.00

• Tris base (0.89M)

• Boric Acid (0.89M)

• Urea (20mM)


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Zymogram Buffer - for protein analysis / use with zymogram

gels to test for proteolytic activity

when characterizing proteins

• 1X: Tris-HCl (63mM), pH 6.8,

SDS (2%), Glycerol (10%),

β-mercaptoethanol (2%),

Bromophenol Blue ( 0.005%)

Sample Buffer

#742162 25ml $ 26.90

#742164 50ml $ 37.50

#765546 500ml $ 14.25

#765547 1L $ 23.75

Renaturation (10X) Buffer• 2.7% (w/v) Triton X-100 in

distilled water

Developing (10X) Buffer• 1X: Tris-HCl (50mM), pH 7.5,

NaCl (200mM), CaCl2 (5mM),

Brij 35 (0.02%)

#765548 500ml $ 19.95

#765549 1L $ 37.15

buffers&reagentsGeneral Use

Acetate Buffer - for biological sciences


• 1M Solution

• DNase, RNase and Protease

free

#700610 4.0 500ml $ 23.75

#700611 4.0 1L $ 37.50

#700612 5.0 500ml $ 23.75

#700613 5.0 1L $ 37.50

pH

Alkaline Phosphatase Buffer (30X)

#730176 50ml $ 24.50

#730177 100ml $ 39.60

• Western Blot development

• Microplate assays

• Tris, Brij -35, NP - 40

• pH 7.6

• Stability: > 6 months at 4°C

Ammonium Acetate Solution - reagent

#705030 250ml $ 19.50

#705032 500ml $ 35.00

• MF: C2H7NO2

• MW: 77.08

• CAS: 631-61-8


free

• 5M Solution

AMP-Buffer

#705522 1L $ 39.60

#705525 4L $ 115.20

• MF: C4H11NO

• MW: 89.14

• CAS: 124-68-5

• High Purity Grade

• 2-amino-2-methyl-1-propanol

• Purity (Anhydrous) >99%

• pH 11.0 - 12.0 at 0.1M

• Storage: RT

• Hazards: Irritant & Combustible

• 5% water has been added to

maintain liquid state at RT.

accelerate: genonics/proteomics>

#705971 1L $ 56.02

#705972 1x1L/ready pk $ 69.50

#765540 500ml (anode) $ 19.95

#765541 1L (anode) $ 35.15

#765542 500ml (cathode) $ 38.00

#765543 1L (cathode) $ 66.50

#765544 500ml kit $ 47.50

#765545 1L kit $ 85.50


• pH (1X) @ 25°C 8.2 +0.1

• Storage: RT


• 1X Anode solution (pH 8.9):

Tris-HCl (200mM)

• 1X Cathode solution (pH 8.3):

Tris base (100mM), Tricine

(100mM), SDS (0.1%)

• TT-SDS Kits include both

Cathode and Anode buffers.

(cat. # 765544 & 765545)

TT SDS (10X)

SDS Running Buffer (10X)

-

BES Buffer

#730285 100ml $ 19.70

#730284 200ml $ 35.00

• MF: C6H15NO5S

• MW: 213.2

• CAS: 10191-18-1

• N, N-Bis(2-hydroxyethyl),

-2- Amino ethane sulfonic acid

• Purity >99%

• pKa range 6.4 - 7.8

• Heavy metals <0.0005%

• Soluble in water

• pH 7.0

• Storage: RT

Tris - Ready Pack - all purpose binding buffer

#700640 10 pk $ 39.20

#700645 40 pk $ 87.20

• Each pack dissolved in

deionized water (yields 500ml

Tris (25mM), NaCl (0.15M)

pH 7.2

Carbonate-Bicarbonate Buffer

#700239 40 pk $ 79.20• Ideal for microplate coating

for RIA & EIA techniques

• Each pack dissolved in 500ml deionized water yields Carbonate-

Bicarbonate buffer (0.2M).

• Total 20 liters

• pH 9.4

Citrate Buffer - great for use with UltraLink® Supports

Citrate-Carbonate


deionized water yields Sodium

Citrate (0.6M), Sodium

Carbonate (0.1M)

#730236 10 pk $ 39.70

• pH 9

Citrate-MOPS


deionized water yields

Sodium Citrate (0.6M), MOPS

(0.1M)

#730269 10 pk $ 41.20

• pH 7.5

Citrate Buffer • MF: C6H8O7

• MW: 192.13

• CAS: 77-92-9

• Purity >99%

• Chloride <0.001%, Oxalate

<0.05%, Phosphate<0.001%,

Sulfate<0.002%, Lead<0.0002%

• Storage: RT

#705168 6.0 250ml $ 23.75

#705170 6.0 500ml $ 39.90

#705172 8.0 250ml $ 23.75

#705174 8.0 500ml $ 39.90

pH

Borate Buffer

#705088 500ml $ 21.95

#705090 1L $ 39.70

#705092 40 pk $ 87.20

Ready Pack• Ideal for protein modification

procedures that require an

alkaline pH.

• Each pack yields Borate (50mM)

• pH 8.5 after adding deionized

water (500ml)

• Makes 20 liters

• MF: H3BO3

• MW: 61.83

• CAS: 10043-35-3

• pKa at 20°C 9.24

• Purity >99.5%

• Sulfate<0.001%

• Calcium<0.005%

• Heavy metals (as Pb) <0.001%

• Iron<0.001%

• Storage: RT

• 50mM Solution

• pH 8.5

MES Buffered Saline - Ready Pack • Designed for use with

carbodiimido coupling

chemistries & Immobilized

DADPA & EDC.

• One pack dissolved in water

yields 500ml MES (0.1M),

NaCl (0.9%)

• pH 4.7

#730270 10 pk $ 72.50

For MES chemical, see page 115

bioPLUS>bestBuffers

buffers&reagents

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EGTA - used in tissue culture media & biological buffers

#700849 50ml $ 35.50

#700850 100ml $ 67.00

• Ethyleneglycol-bis

(2-Aminoethylether)-N,N,N’,N’

tetraacetic acid

• MF: C14H24N2O10

• MW: 380.35

• CAS: 67-42-5

• Purity (by titration) >97%

• Melting point (with

decomposition) 238°C

• 0.5M Solution

• pH 8.0

• Storage: RT


• Metal chelating agent specific for

calcium ions

Glycine Buffer - for biological sciences

#700616 250ml $ 23.75

#700617 500ml $ 39.90

• MF: C2H5NO2

• MW: 75.07

• CAS: 56-40-6

• Purity>99%

• Concentration (0.2M)

• pH 3.0

• A280 (water 1.0M)<0.1

• Heavy Metals (as Pb)<0.002%


• Storage: RT

Phosphate Buffer - for biological sciences

#700658 4.4 500ml $ 23.75

#700659 4.4 1L $ 39.90

#700661 6.8 500ml $ 23.75

#700662 6.8 1L $ 39.90

#700664 7.0 500ml $ 23.75

#700665 7.0 1L $ 39.90

#700666 7.2 500ml $ 23.75

#700667 7.2 1L $ 39.90

#700668 9.1 500ml $ 23.75

#700669 9.1 1000ml $ 39.90

• High Purity

• Concentration (0.2M)

• pH +0.1

• Storage: RT

pH

-

DEPC-Treated Water -for ultimate RNA preparations

#700799 250ml $ 18.70

#710147 500ml $ 27.20

#710148 1L $ 36.00

• Ultra Pure Water

• Molecular Grade

• Treated with - DEPC

PBS (Phosphate-Buffered Saline)

Ready Pack (10X) - powder blend

#730095 1 x 1L/pk $ 19.04

#730096 2 x 1L/pk $ 31.50

#730097 5 x 1L/pk $ 69.70


• pH (at 25˚C) 7.4 (+0.1)



free

1X- #705837 100ml $ 17.43

#705838 500ml $ 46.19

20X- #730100 500ml $ 26.40

#730102 1L $ 44.42

#700642 2L $ 59.00

#730098 4L $ 82.43

PBS-Liquid Concentrate


• 1X-dissolve 9.88g in distilled

water (1L)

• pH (9.88g/L water) 7.4

• Clear / haze-free

• Sodium Chloride (137mM)

• Potassium Chloride (2.7mM)

• Phosphate Buffer (10mM)

• Storage: RT

-


MOPS

SDS Running Buffer (20X)

#743148 500ml $ 41.90

#743149 1L $ 69.70

#705760 250ml $ 44.37

MOPS Buffer (10X)• Biotechnology Grade

• Prepared with DEPC-treated

double distilled H2O for RNA

applications

• pH (at 25°C) 6.8-7.2

• RNase: None detected

• DNAse: None

• Storage: RT


• 1X concentration: MOPS

(50mM), Tris base (50mM),

SDS (0.1%), EDTA (1mM)

• pH 7.7

Potassium Acetate - biological buffer

#700875 1 100ml $ 14.70

#700876 1 200ml $ 26.50

#700872 3 100ml $ 19.50

#700873 3 250ml $ 35.00

*M

* M = molarity

• MF: KC2H3O2

• MW: 98.14

• CAS: 127-08-2

• ACS Grade

• pH (at 25˚C, water 5%) 6.5 - 6.9

• Storage: RT

• Purity>99%

• Sodium<0.03

• Chloride<0.003%

Sodium Bicarbonate

• MF: NaHCO3

• MW: 84.01

• Sodium Hydrogen Carbonate

• 1M Solution

• pH 8.0 (+/-0.1)

• Storage: RT

#700675 500ml $ 23.75

#700676 1L $ 39.90

#700647 500ml $ 15.20

#700648 1L $ 26.60

PBS Dulbecco’s - for in vitro use • Modified washing liquid

• Cell culture tested

• Store 2-8˚C

#700650 500ml $ 23.75

#700651 1L $ 39.90

#730091 1x100ml $ 36.40

#730093 2x100ml $ 57.40

PBS Tablets • Ultra Pure Grade


• Phosphate Buffer (10mm)

• Potassium Chloride (2.7mm)

• pH at 25˚C 7.4 (+0.1)

• Storage: RT


• Tablets dissolve within 3

minutes with stirring

• Higher concentrations obtained

by dissolving 2, 3 or 4 tablets in

water (100ml)

• To sterilize the solution for cell

culture, autoclave or sterile filter.

#700643 500ml $ 17.18

#700644 1L $ 26.60

PBS With Ca2+ & Mg2+ (10X) - for peroxidase conjugates • High Purity Grade

• Sodium Phosphate (100mM)

• NaCl (9%)

• Ca2+ & Mg2+ added

• pH (at 25˚C) 7.4

- for peroxidase conjugates


• NaCl (9%)

• pH (at 25˚C) 7.4

-

#700878 100ml $ 13.50

#700879 200ml $ 20.00

#700880 500ml $ 35.00

Potassium Chloride - reagent for molecular biology

• MF: KCl

• MW: 74.55

• CAS: 7447-40-7

• ACS Grade

• 1M Solution

• Purity 99.5%• pH at 25˚C water (5%) 5.4 - 8.6

• Storage: RT

Potassium Phosphate - buffer for biological sciences


• 1M Solution


• NaCl (9%)

• Storage: RT

#700654 7.2 500ml $ 23.75

#700655 7.2 1L $ 39.90

#700656 7.4 500ml $ 23.75

#700657 7.4 1L $ 39.90

pH

pH

Sodium Acetate (sterile)

#700890 4.5 250ml $ 19.50

#700891 4.5 500ml $ 35.00

#700893 5.2 200ml $ 25.94

#700894 5.2 500ml $ 35.00

#700896 7.0 250ml $ 19.50

#700897 7.0 500ml $ 35.00

• Autoclave Sterilized

• 3M solution

• Storage: 4˚C

• Biotechnology grade


buffers&reagents

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STET Buffer

#730014 500ml $ 26.00

#730010 1L $ 34.90



• Tris HCl (10mM)

• EDTA (1mM)

• Triton-X 100 (5%)

• Conductivity (at 25˚C)

13.0-14.0 umhos

• pH (at 25˚C) 7.90 - 8.10

• Storage: RT


Sodium Cacodylate Buffer

• MF: C2H6AsNaO2

• 1M solution

• High Purity (98%)

• pH 6.8 (+0.1)

#700687 6.8 500ml $ 23.75

#700688 6.8 1L $ 39.90

#700690 7.0 500ml $ 23.75

#700691 7.0 1L $ 39.90

pH

-

#700679 500ml $ 37.50

#700680 1L $ 55.60

Sodium Borate

• MF: BNaO2

• MW: 65.80

• 1M Solution

• pH 9.0 (+/-0.1)

#700693 5.8 500ml $ 19.70

#700694 5.8 1L $ 29.50

#700696 6.0 500ml $ 19.70

#700697 6.0 1L $ 29.50

#700699 6.2 500ml $ 19.70

#700700 6.2 1L $ 29.50

#700702 6.4 500ml $ 19.70

#700703 6.4 1L $ 29.50

#700705 6.6 500ml $ 19.70

#700706 6.6 1L $ 29.50

#700708 6.8 500ml $ 19.70

#700709 6.8 1L $ 29.50

#700711 7.0 500ml $ 21.70

#700712 7.0 1L $ 32.50

#700714 7.2 500ml $ 19.70

#700715 7.2 1L $ 29.50

#700717 7.4 500ml $ 19.70

#700718 7.4 1L $ 29.50

#700720 7.6 500ml $ 19.70

#700721 7.6 1L $ 29.50

Sodium Phosphate Buffer • Buffer solution from

Phosphoric Acid / mono-

Sodium / DiSodium

Hydrogen Phosphate

• 1M Solution

• Prepared under clean

room conditions

• Limited Shelf Life

• Used for protein purification,

immunological assays and for

cell cultures

#700683 500ml $ 19.70

#700684 1L $ 29.50

Sodium Citrate Buffer • High Purity Grade

• Buffer solution from Sodium

citrate

• 1M Solution

• pH 5.5 (+0.1)-

#700900 100ml $ 17.50

#700901 200ml $ 27.50

#700903 500ml $ 49.00

Sodium Hydroxide (10N) - reagent for alkaline lysis & pH

• MF: NaOH

• MW: 40.00

• CAS: 1310-73-2

• ACS Grade

• Purity>97%

• Sodium Carbonate<1%

• Heavy metals (as Pb)<0.002%

• Storage: RT


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Tris-NaCl-Tween (TNT Buffer)

#700941 1L $ 19.50

#700942 2L $ 35.00


• Tris-HCl (10mM)

• NaCl (150mM)

• Tween 20 (0.05%)

• pH 8.0


19.00-20.50 umhos

• Storage: RT

buffers&reagentsImmunology

Sucrose Solution - reagent used for immunology

#730260 500ml $ 19.50

#730286 1L $ 35.00

• MF: C12H22O11

• MW: 324.30

• CAS: 57-50-1


• Purity >99.9%

• 1.2M Solution

• Specific rotation +66.3 - +66.8˚

• Glucose <0.1%

• Chloride <0.005%

• Heavy metals (as Pb) <0.0005%

• RNAse, DNAse: None

• Storage: 4˚C

TM Buffer

#730016 500ml $ 26.88• Ultra Pure Grade

• Tris-HCl (50mM)

• Magnesium Sulfate (8mM)

• pH at 25˚C 7.4 - 7.6


5.70 - 6.70 umhos

• DNase: Free

• Storage: RT

Tris-Phosphate-EDTA (10X)

#700960 1L $ 19.50

#700961 2L $ 35.00

#700962 4L $ 65.00


• Tris base (0.9M)

• Phosphate (0.9M)

• EDTA (20mM)

TN Buffer - for molecular biology

#760005 1 x 500ml $ 13.50

#760006 1x1L $ 19.50

#760009 10 x 1L $ 49.50


• Tris-HCl , (10mM)

• NaCl (150mM)

• pH 8.0

Borate Buffer Saline (BBS) Reagents

Fish Gelatin

#715396 2 x 100ml $ 49.50

#715395 500ml $ 67.00

• Gelatin (5%)

• Diluents & coating solutions

supplied as 10X concentrates

• pH 8.0 (+0.1)

formulated in BBS

containing glycerol

#715397 2 x 100ml $ 62.10

#715393 500ml $ 130.75

Fish Gelatin-Glycerol

Casein• 10X has a working

pH of 8.5 (+0.1)

• Formulated in BBS

• Enhance sensitivity of ELISA

assays

#715381 2 x 100ml $ 49.70

#715386 500ml $ 69.80

Casein-Glycerol• 10X concentrate has a working

pH of 7.8 at 1:10

pH of 8.2 at 1:20

• Formulated in BBS and

glycerol

#715382 2 x 100ml $ 59.70

#715379 500ml $ 78.50

• Gelatin (5%)

• Diluents & coating solutions

supplied as 10X concentrates

• pH 8.0 (+0.1)

formulated in BBS saline

Casein Blocker with Fish • 10X concentration

• 30ml of each of the eight 10x

concentrations

• Prepares 2.4L

#715389 6 x 30ml $ 49.50

-

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Blotto Blocker Buffer - to block non specific binding for

antibodies on the membrane Blotto / in PBS

#740031 500ml $ 21.70

#740032 1L $ 37.50

#740078 250ml $ 23.75

#740079 500ml $ 38.00

Blotto / Anti-Foam in PBS

Blotto / Tween in PBS

#740082 250ml $ 21.70

#740083 500ml $ 37.50

Blotto / in TBS

Blotto / Anti-Foam in TBS

#740084 250ml $ 23.75

#740085 500ml $ 38.00

#740029 500ml $ 21.70

#740030 1L $ 37.50

• Nonfat dry milk (5%)

• Sodium Azide in PBS (0.02%)



• Antifoam A (0.01%)



• Tween (0.05%)


• Sodium Azide in TBS (0.02%)

• Antifoam A (0.01%)



Bicarbonate Buffer

#705728 8.0 500ml $ 19.70

#705708 8.0 1L $ 34.50

#705706 9.0 500ml $ 19.70

#705707 9.0 1L $ 34.50

#700233 10.0 500ml $ 19.70

#700234 10.0 1L $ 34.50

• MF: NaHCO3

• MW: 84.0

• CAS: 144-55-8

• 50mM Solution


pH

accelerate: immunodetections>

Imidazole Buffered Saline(IBS) Reagents IBS Casein

#715390 2 x 100ml $ 62.70

IBS Fish Gelatin

IBS Fish Gelatin-Glycerol

#715391 1L $ 149.00

#715383 2 x 100ml $ 47.50

#715384 500ml $ 69.50

• All blocks and diluents are

supplied in a 10X concentration

of physiological saline buffer.

• pH 7.0 (+0.1) based upon an

imidazole physiological buffer

• Gelatin (5%)

• Diluents and coating

solutions supplied as 10X

• pH range 7.0 (+0.1)

• Gelatin (5%)

• pH range 7.0 (+0.1)

-

-

-

Phosphate Buffer Saline (PBS) Reagents

PBS Casein (10X)

#715387 2x100ml $ 49.70

#715338 500ml $ 69.50

• Gelatin (5%)

• 10X concentrate

• pH range 7.4 (+0.1)

PBS Fish Gelatin (10X)

• All blocks & diluents are

supplied in a 10X PBS

• pH range 7.4 (+0.1)

#715392 2x100ml $ 49.70

#715401 500ml $ 69.50

-

-

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BSA Blocking Buffer - to block non specific binding site for

antibodies on the membrane

BSA in PBS (10X)

BSA in TBS (10X)

• BSA in PBS (3%)

• Sodium Azide (0.02%)

• BSA in TBS (3%)


#740025 100ml $ 32.50

#740026 200ml $ 49.70

#740027 100ml $ 32.50

#740028 200ml $ 49.70

Blotto / Tween in TBS

#740087 250ml $ 21.70• Nonfat dry milk (5%)


• Tween-20 (0.05%)

Carbonate Buffer

#700235 500ml $ 19.50

#700236 1L $ 27.50

• MF: Na2CO3

• MW: 106.0

• CAS: 5968-11-6


• Sodium Carbonate

• 0.05M Solution

• pH 9.4

Casein Blocking Buffer - to block non specific binding

site for antibodies on the membrane

Casein in PBS

Casein in PBS with Azide

• Casein PBS (3%)

• Casein (3%)


#740033 500ml $ 23.75

#740034 1L $ 38.00

#740060 500ml $ 28.50

#740061 1L $ 49.70

Casein in TBS• Casein (3%)

#740035 500ml $ 28.50

#740042 1L $ 43.50

Casein in TBS with Azide• Casein (3%)

• Sodium Azide (0.02%)#740063 500ml $ 28.50

#740064 1L $ 49.60

Gelatin in PBS with Azide

• Gelatin (1%)


#740068 500ml $ 28.50

#740069 1L $ 52.25

Gelatin / Tween in PBS• Gelatin 10%


• Tween 20 (0.05%)

#740098 500ml $ 27.50

#740099 1L $ 47.50

#740074 500ml $ 28.50

#740075 1L $ 49.70

Gelatin in TBS with Azide• Gelatin (1%)


Gelatin / Tween in TBS• Gelatin (10%)


• Tween 20 (0.05%)

#740100 500ml $ 27.50

#740101 1L $ 47.50

#740072 500ml $ 28.50

#740073 1L $ 49.70

Gelatin in TBS• Gelatin (1%)

Gelatin Blocking Buffer

Gelatin in PBS

• Gelatin 0.1%#740066 500ml $ 28.50

#740067 1L $ 52.25

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PBS-T (Tween 20) - phosphate buffered saline with Tween 20 to block non specific binding of proteins.

PBS-T (Tween 0.5%) 10X

#730104 250ml $ 23.75

#730108 500ml $ 38.00

PBS-T (Tween 1%) 10X

#730110 500ml $ 23.75

#730112 1L $ 38.00


• pH (at 25°C) 7.4 - 7.6

• Storage: RT



• pH (at 25°C) 7.5

• Storage: RT


Protein Stripping Buffer (4X) - blot stripping buffer

with Tris

#740121 250ml $ 19.70

#740122 500ml $ 34.50

with Glycine

#740123 250ml $ 17.80

#740124 500ml $ 32.50


• Tris (250mM)

• SDS (8%)

• Add ß-mercaptoethanol

• pH at 25°C 6.8



• Glycine (200mM)

• pH at 25°C 2.5

TBS-T (Tween 20) 10X - to block non specific binding site for

antibodies on the membrane

0.5%- #740092 500ml $ 21.50

#740093 1L $ 37.50

1%- #740090 500ml $ 21.50

#740091 1L $ 37.50

• Tween 20 in 10x TBS

(Tris Borate Saline)

Para-Formaldehyde (4%) - reagent for histology

In PBS

In PBS with Mg2+, EGTA


• Storage: 2-8°C


• Storage: 2-8°C

#730280 250ml $ 27.60

#730281 500ml $ 48.50

#730282 250ml $ 27.60

#730283 500ml $ 48.50

Horse Blocker

#740104 100ml $ 33.25

#740105 200ml $ 57.00

• Horse serum (10%)


accelerate: immunodetections>


Formaldehyde (4%) IN PBS - reagent for histology

#730278 250ml $ 19.50

#730279 500ml $ 35.00


• Storage: 2-8°C

• Provided as 10x concentration.

• Dilute to your working

concentration.

Formulated to be an effective running buffer when used with automated

sequencers. This optimized buffer concentrate provides excellent results,

long read lengths,and consistent run times.

Capillary Sequencing Buffer

#700963 100ml $ 25.00

#700965 500ml $ 450.00

#700964 1L $ 120.00

buffers&reagentsCapillary Electrophoresis

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Tetraborate Buffer pH 9.9-10, 1mM

#700968 100ml $ 19.00

#700969 250ml $ 39.00

Tricine Buffer pH 7.0, 1M

#700968 250ml $ 23.75

#700969 500ml $ 39.00

Acetate Buffer pH 7.0, 1M

#700659 500ml $ 23.75

#700661 1L $ 39.00


buffers&reagentsProtein Purification

CHAPS Lysis Buffer (1X) - for extracting protein from cells

#765531 250ml $ 33.25

#765532 500ml $ 57.50


• NaCl (110mM)

• EDTA (5mM)

• CHAPS (1%)

• See Cat#730039 for CHAPS

DEA Buffer (10X)

#730148 50ml $ 28.40

#730149 100ml $ 42.90

• Diethanolamine & MgCl2

• pH 9.5

• Stability >1 year at 4°C

Denaturing Lysis Buffer (1X) - for extracting proteins

#765529 250ml $ 16.50

#765530 500ml $ 27.00


• SDS (2%)

E. coli Lysis Buffer II - for protein extraction & purification

of GST-fusion proteins

#700202 7.4 250ml $ 19.50

#700203 7.4 500ml $ 35.00

#700200 8.0 250ml $ 19.50

#700201 8.0 500ml $ 35.00


• Triton X-100 (1%)

• EDTA (1mM)

• β-mercaptoethanol (10mM)

• KCl (2.7mM)

• NaCl (140mM)

• See also Cell Buster BL Cat #506150

pH

accelerate: proteinpurification>

Phosphate Buffer

#700659 500ml $ 23.75

#700661 1L $ 39.00

#700662 500ml $ 23.75

#700664 1L $ 39.00

pH 4.4, 0.2M

pH 6.8, 0.2M

CAPS Buffer pH 9.9-10, 1mM

#700966 100ml $ 19.00

#700967 250ml $ 39.00

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Gelatin (10%) - to block non specific binding site for antibodies on

the membrane

#740096 500ml $ 33.25

#740097 1L $ 57.00

• Ready to use

• Gelatin (10%) solution

• For fish gelatin (see page 77)

MES Buffer - used in plant cell culture

#700628 6.1 250ml $ 23.75

#700629 6.1 500ml $ 39.90

#700631 6.5 250ml $ 23.75

#700632 6.5 500ml $ 39.90

• MF: C6H13NO4S

• MW: 195.2

• CAS: 4432-31-9


• pH range 5.5 - 6.7

• pKa at 25°C 6.10 (+0.05)

• 1M Solution

• Storage: RT

• pKa value near physiological

pH- not absorbed through cell membranes.

• Essentially transparent to UV light

• For MES Chemical (see page 107)

pH

-

��

��

��

��©

EIA Buffer (10X) Concentrate - makes 500ml of 1X

solution when diluted with distilled water #730139 50ml $ 24.40

#730190 100ml $ 37.00

• Solution contains 1% BSA,

4M NaCl, 10mM EDTA and

0.1% Azide

• 1M Phosphate Solution

• pH 7.4

• Stability >1 year at 4°C

#765535 250ml $ 23.75

#765536 500ml $ 37.50

Protein Extraction (5X) - for extracting proteins from cells

• HEPES (250mM)

• CaCl2 (5mM)

• pH 7.4

• Add protease inhibitor according to your protocol.

Triton Protein Extraction • HEPES (50mM)

• Triton X-100 (10%)

• pH 7.4

• Add protease inhibitor according

to your protocol

#765537 250ml $ 23.75

#765538 500ml $ 38.00

HEPES 1M Sterile pH 7.3 • Biotechnology Grade

• MF: C18H18N2O4S

• DNAse, RNase, Protease free

• pH (at 37°C) 7.2 - 7.4

• Storage: RT

• For HEPES Chemical (see page 106)

#730048 100ml $ 18.27

#730050 500ml $ 47.35

#700620 250ml $ 28.50

#700621 500ml $ 49.70

HEPES Buffered Saline (2X) - for biological sciences

• NaCl (280mM)

• KCl (10mM)

• Na2HPO4 (1.5mM)

• Dextrose (12mM)

• HEPES (50mM)

• pH 7.01

HEPES Buffer & Reagents

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PIPES Buffer 1M - for cell culture, in vitro & other

biological applications

#700635 100ml $ 25.75

#700636 200ml $ 44.30

• MF: C8H17N2O6S2 *Na 1.5

• MW: 335.37

• CAS: 100037-69-2


• pKa at 20°C 6.80

• pH range 6.1 - 7.5

• Purity >99%

• Storage: RT

• pKa value near physiological pH

• Not absorbed through cell

membranes

• Essentially transparent to

UV light

Protein Extraction/Purification Buffer

Extraction

E. coli Lysis

• Tris-HCl (50mM)

• NaCl (500mM)

• Triton X-100 (1%), pH 8.0

• For protein extraction &

purification of GST fusion proteins

• MF: Na2HPO4 (50mM)

• NaCl (300mM)

• Imidazol (10mM), pH 8.0

• For His-tag protein purification

from E. coli & insect cells

#700206 250ml $ 19.50

#700207 500ml $ 35.00

#700208 250ml $ 19.50

#700209 500ml $ 35.00

E. coli Wash• MF: Na2HPO4 (50mM)

• NaCl (300mM)


#700210 250ml $ 19.50

#700211 500ml $ 47.50

Protein Precleaning Buffer - for clarification of

proteins to eliminate nucleic acids & polysaccharides before electrophoresis

#730026 10 x 1ml $ 29.95

#730027 2 x 10ml $ 37.70

• For use with 1D gels

• Storage: 20°C

• Total protein preparation &

cleaning solution

MOPS Buffer - for culturing cells in-vitro & other biological

applications MOPS-1M pH 7.0

MOPS-EDTA (10X)


• 3-(N-Morpholino)

Propane Sulfonic Acid

• MF: C7H15NO4S

• pH range 6.8 - 8

#700626 125ml $ 23.75

#700627 250ml $ 39.90

• pKa at 25°C: 7.0 - 7.4

• Purity >99%



• MOPS (0.4M)

• Sodium Acetate (0.1M)

• EDTA (0.01M)

• RNAse: None

• DNAse: None

• pH at 25°C 6.8 - 7.2

• Storage: RT

• Prepared with DEPC-treated

double distilled H2O for RNA

applications

#730068 100ml $ 25.62

#730070 250ml $ 34.44

#730072 500ml $ 49.50

NP-40 Lysis Buffer - for extracting proteins from cells

No Salt

#765523 250ml $ 23.75

#765524 500ml $ 38.00

Low Salt


• Tris-HCl (50mM)

• NP-40 (1%)

• EDTA (5mM), pH 8.0

#765527 250ml $ 23.75

#765528 500ml $ 38.00

#765525 250ml $ 23.75

#765526 500ml $ 38.00

High Salt


• Tris-HCl (50mM)

• NP-40 (1%), EDTA (5mM)

• NaCl (500mM), pH 8.0


• Tris-HCl (50mM)

• NP-40 (1%)

• EDTA (5mM), pH 8.0

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RIPA Buffer - for extracting proteins from cells

RIPA pH 7.4

1X- #765512 250ml $ 29.70

#765513 500ml $ 49.50

2X- #765504 250ml $ 27.50

#765505 500ml $ 44.70

RIPA pH 8.0 (1X)

RIPA with EDTA (1X)

#765506 250ml $ 29.70

#765507 500ml $ 49.50

RIPA with EDTA & EGTA

#765510 250ml $ 29.70

#765511 500ml $ 49.50

• Tris-HCl (50mM)

• NaCl (150mM)

• NP-40 (1%)

• Sodium Deoxycholate (0.5%)

• SDS (0.1%)

• EGTA (1mM) / EDTA (5mM)

RIPA with Triton X-100

1X- #765516 250ml $ 29.70

#765517 500ml $ 49.50

5X- #765514 250ml $ 29.70

#765515 500ml $ 57.25

• 1X - Tris-HCl (50mM), pH 7.4,

NaCl (150mM), Triton X-100

(1%), Sodium Deoxycholate

(0.5%), SDS (0.1%)

• 5X - Tris-HCl (250mM), pH 7.4,

NaCl (750mM), Triton X-100

(5%), Sodium Deoxycholate

(2.5%), SDS (0.5%)

• Tris-HCl (50mM)

• NaCl (150mM)

• NP-40 (1%)


• SDS (0.1%)

#765502 250ml $ 29.70

#765503 500ml $ 49.50

• EGTA (5mM)

• pH 7.4

• Tris-HCl (100mM)

• NaCl (300mM)

• NP-40 (2%)


• SDS (0.2%)

• Tris-HCl (50mM)

• NaCl (150mM)

• NP-40 (1%)


• SDS (0.1%)

#700217 250ml $ 19.50

#700218 500ml $ 35.00

Mammalian Protein Elution

• Na2HPO4 (50mM)

• NaCl (300mM)

• Imidazol (250mM)

• Tween 20 (0.5%), pH 8.0

• For His-tag protein purification from mammalian cells

Protein Coupling Kit

• Gel swelling solution:

HCl (1mM)

• Gel coupling solution-Sodium Borate or Citrate (100mM),

NaCl (500mM), pH 9.3

• Gel washing solution-Sodium Acetate (100mM),

NaCl (500mM),pH 4.0

• Kit is used for CnBr-activated Sepharose™ / Sepharophore™

Coupling.

#730265 1 Kit $ 93.00

#700212 250ml $ 19.50

#700213 500ml $ 35.00

E. coli Protein Elution

• Na2HPO4 (50mM)

• NaCl (300mM)


• For His-tag protein purification from E. coli & insect cells

#700219 250ml $ 19.50

#700220 500ml $ 35.00

Mammalian Protein Wash

• Na2HPO4 (50mM)

• NaCl (300mM)

• Imidazol (20mM)

• Tween 20 (0.5%), pH 8.0

• For HIS-TAG protein purification from mammalian cells

• EGTA (1mM) / EDTA (5mM)


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Sodium Dodecyl Sulfate (20%) - ionic detergent used for denaturation of native proteins

#700906 250ml $ 22.80

#700907 500ml $ 39.70

• MF: C12H25NaO4S

• MW: 288.38

• CAS: 151-21-3


• Aggregation #: 62

• Purity >99%

• A260 (3% water)<0.1

• A280 (3% water)<0.1

• RNase, DNase: None

• Storage: RT

• Chloride<0.1%

Thrombin Buffer - for protein extraction & purification of GST

fusion proteins

#700204 250ml $ 22.50

#700205 500ml $ 39.25

• Tris-HCl (50mM)

• NaCl (140mM)

• CaCl2 (2mM), pH 8.0

Tricine Buffer - for biological sciences

#730184 250ml $ 23.75

#730186 500ml $ 39.90


• 1M solution

• DNase: None

• Causes irritation to eyes, skin & mucus membranes if inhaled

or ingested.

TBS (Tris Buffered Saline) - for molecular biology &

biochemistry

TBS (10X) pH 7.2

TBS (20X) • Ultra Pure Grade

• Tris (500mM)

• pH (at 25°C) 7.25 - 7.55

• Storage: RT


#730230 1L $ 17.75

#730228 2L $ 35.15

TBS (10X) pH 7.4 • DNase: None

• Causes irritation to eyes, skin,

& mucus membranes if inhaled

or ingested.


• Tris (250mM)

• pH (at 25°C) 7.25 - 7.55

• Storage: RT


#740108 1L $ 19.95

#740109 2L $ 35.15

#740110 4L $ 66.50

#730231 2x1L/ready pk $ 26.25

#730232 4L $ 71.40

#730233 2xquick mix pk $ 26.25

Tris Buffers

Tris Acetate

Tris-CAPS (10X)


• Sterile

• 1M solution

• For the transfer of proteins

from gel onto the membrane

• Anode buffer-Tris (0.6M), CAPS

(0.4M), pH 9.6

• Cathode buffer-Tris (0.6M),

Caps (0.4M), SDS (1%), pH 9.5

#730188 7.6 1L $ 29.30

#730189 8.5 1L $ 24.50

#765550 500ml $ 23.75

#765551 1L $ 42.75

Tris HCl • Biotechnology Grade

• DNase: None

• 1M Solution

• pH + 0.1

• Causes irritation

to eyes, skin &

mucus membranes if

inhaled or ingested.

• Wash thoroughly

after handling

#700724 6.8 1L $ 23.85

#700725 7.0 1L $ 23.85

#700726 7.2 1L $ 23.85

#700727 7.4 1L $ 23.85

#700728 7.5 1L $ 23.85

#700729 7.6 1L $ 23.85

#700730 7.8 1L $ 23.85

#700731 8.0 1L $ 23.85

#700732 8.2 1L $ 23.85

#700733 8.4 1L $ 23.85

#700734 8.6 1L $ 23.85

#700735 8.8 1L $ 23.85

pH

pH

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Tris HCl (Sterile) • Biotechnology Grade

• Storage: RT


* M = Molarity

#730214 0.5 6.8 500ml $ 29.72

#730206 0.1 7.4 100ml $ 17.43

#730218 0.1 7.4 500ml $ 27.51

#730199 1 7.5 100ml $ 23.52

#730195 1 7.5 500ml $ 41.50

#730212 2 7.5 1L $ 87.84

#730213 2 7.8 500ml $ 44.85

#730194 1 8.0 100ml $ 16.38

#730197 1 8.5 500ml $ 18.27

#730198 1 9.0 250ml $ 21.48

#730216 1 10 250ml $ 19.53

#765533 250ml $ 23.75

#765534 500ml $ 38.00

Protein Extraction (5X) • Tris-HCl (0.5M)

• β-Mercaptoethanol (100mM)

• EDTA (20mM)

• pH 8.0

• Add protease inhibitor according to your protocol

pH*M

Triton Cell Lysis Buffer - for extracting proteins from cells

#765518 7.4 250ml $ 19.70

#765519 7.4 500ml $ 34.50

#765521 8.0 250ml $ 19.70

#765522 8.0 500ml $ 29.70


• Tris-HCl (50mM)

• NaCl (150mM)

• Triton X-100 (1%)

• EDTA (5mM)

pH

accelerate: proteinpurification>

• Ultrapure DTT for

protein reduction.

• Add an aliquot in buffer

solution to maintain protein

reduction potential.

• Protease free for proteomics

work. For solid DTT see Catalog# 705323, page112)

#515939 5 x 1ml $ 37.40

#515929 10 x 1ml $ 49.00

DTT (2M)

electrophoresis - bioworld · electrophoresis 68 buffers & reagents for protocols visit...

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