electron transport & oxidative...
TRANSCRIPT
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Aerobic RespirationElectron Transport
& Oxidative Phosphorylation
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Electron Transport Chain
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The Electron Transport Chain・ the final stage of aerobic cellular respiration
・ takes place on the inner membrane of the
mitochondria ・ ETC extracts the potential energy from NADH &
FADH2
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The Electron Transport Chain・ electrons are transferred from NADH & FADH2 (produced
in earlier stages) to final electron acceptor of oxygen
・ ETC made up of 4 protein complexes (I, II, III, IV) & 2
mobile electron shuttles
・ electrons are pulled along protein complex because each electron carrier is more electronegative then the previous carrier (i.e., NADH has the weakest hold on electrons, Oxygen the strongest)
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・ NADH enters the ETC at complex I
・ FADH2 enters at complex II (which is why each NADH produces
more ATP than FADH2 - 3 versus 2)
・ water is produced when oxygen accepts electrons in the final step
NOTE: ETC does not directly produce any ATP… for ATP production go to ATP Synthase
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ATP Synthase Complex
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Oxidative Phosphorylation
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Adding it all up…For every NADH that is oxidized… • 10 H+ ions are pumped into the inner membrane space • 3-4 H+ ions are needed to flow through ATP synthase to
make 1 ATP 1 NADH results in a maximum of 3 ATP
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…keep adding…• FADH2 bypasses complex I, so fewer H+ are pumped
across • 1 FADH2 results in a maximum of 2 ATP
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Theoretical Energy YieldGlycolysis: Substrate-level phosphorylation 2 ATP 2 NADH ! 6 ATP Pyruvate Oxidation: 2 NADH ! 6 ATP
Krebs Cycle: Substrate-level phosphorylation 2 ATP 6 NADH ! 18 ATP 2 FADH2 ! 4 ATP TOTAL: 38 ATP