d.y.patil department of biotechnology syllabus

7/30/2019 D.Y.Patil department of biotechnology syllabus

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B. Tech (Biotechnology)

Sem I Sem II Sem III Sem IV Sem V Sem VI

Paper

I

CellBiology

Concept ofBiotechnology

Microbiology IntermediaryMetabolism -

I

MolecularCell

Signalling

Neurobiologyand Behavioral

Sciences

Paper

II

Biomathematics Genetics &Evolution

Molecules oflife

Enzymology& Enzyme

Technology

IntermediaryMetabolism II

EnvironmentalTechnology

Paper

III

EngineeringMechanics

EngineeringDrawing

Biological &Biochemical

Techniques

MolecularBiology of

Prokaryotes

CommercialAspects of

Biotechnology

BioprocessTechnology

Paper

IV

Ecology &

Biodiversity

Developmental

Biology

Mammalian

Anatomy

Molecular

Biology ofEukaryotes

Plant

Biotechnology

Animal

BiotechnologyE

Paper

V

AppliedPhysics

ElectronicsEngineering

Mammalianphysiology

Advancedanalytical

techniques

GeneticEngineering- I

(Principles)

AppliedGenetic

Engineering-II

(Application)

G

Paper

VI

Applied

chemistry

Biostatistics Biophysics Immunology

& Virology

Bioinformatics

I(Principles)

Bioinformatics

II(Application)


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B.Tech. (Biotechnology)

BBT - 101 CELL BIOLOGY 40hrs

UNIT 1:Introduction, History and general concepts - 02

Levels of Organization, Limits & Dimensions,History

UNIT 2: General Structure of Cells 04

Prokaryotic, Eukaryotic , Plant, Animal

UNIT 3: Ultra structure of Cell membrane and Cell wall 04

Chemical Composition, Models, Functions

UNIT 4: Ultra structure of Cytoplasm and Cytoplasmic organelles 18

Cytosol, Golgi bodies, Endoplasmic retch, Mitochondria, Ribosomes,

Lysosomes, Peroxisomes, Nucleus, Cytoskeleton, Cilia, Flagella, Chloroplast

UNIT 5: Cell division 06

Mitosis, Meiosis

UNIT 6: Apoptosis 03

R e c o m m e n d e d B o o k s : -

1. Cell and Molecular biology, Gerald Karp, John Wiley and sons Inc

2. Cell Biology by C.B. Powar.

3. Cell and Molecular Biology; DeRobertis; Lippincott Williams & Wilkins 8th

Edition (2001)

4. Molecular Biology of the Cell and the Hypercell with CDROM; Alberts, Bray;

Garland Publishing 1st

Edition (1999)


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5. Molecular Biology of the Cell with CDROM Alberts, Bruce; Johnson,

Alexander; Lewis, Julian 4th

Edition (2005).

6. Molecular Cell Biology , H. Lodish, A. Berk, S. L. Zipursky, W. H. Preeman and

Company.


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BBT 102 BIOMATHEMATICS 40hrs

UNIT 1: Set, Functions and Limit 05

Definition, Types of Set: Null Set, Universal Set. Operations on Set: Union,

Intersection, Complementation, Relative Difference, De-Morgans Law.

Problems of each. Functions definitions: Types of Functions.Limit of Simple

Algebraic Function

UNIT 2 Derivative and Integration 10

Derivatives of :Simple Function,Logarithmic Function, Trigonometric Functions,

(u +v , u-v, uxv, and u/v).Integration of :Simple Function, Logarithmic Function,

Trigonometric Functions, (u +v , u-v, uxv, and u/v)

UNIT 3 :Theory of Probability 10

Definitions of various terms, Mathematical or Classical Probability, Statistical or

Empirical Probability, Axiomatic Probability. Problems ; Mathematical Notions

of Probability,General Addition Law of Probabilities,General Multiplication Law

of Probabilities, Conditional Probability, Theorem of Total Probability with proof,

Bayes Theorem with proof. Proble

UNIT 4:Random Variable and Probability Distribution 10

Frequency Distribution: Discrete Distribution, Continuous Distribution.

Conversion from discrete to continuous. Random Variable, Probability Mass

Function, Probability Density Function.Problems.Definition, Situations and

Problems of: Binomial Distribution, Poisson Distribution, Normal

Distribution.Recurrence Relations and Fitting of: Binomial Distribution, Poisson

Distribution, Normal Distribution, Expectation of R.V, ( expectation of a function

of a r.v. should be defined. For all the above distributions using these definitions

mean & variance should be obtained).Problems

UNIT 5: Curve Fitting 07

Curve Fitting: Discussion,Fitting of straight line, Fitting of second degree

parabola, Fitting of kth

degree, Change of origin, Conversion of data to Linear

Form, Problems wherever required, Fitting of Power Curve, Fitting of

Exponential Curve


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Recommended Books:

1. Fundamentals of mathematical Statistics.

2. Fundamentals of Applied l Statistics.

3. Statistical Method.

4. Probability and Random Process


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BBT- 103 ENGINEERING MECHANICS 40hrs

UNIT 1: Basics 03

Introduction - Units and Dimensions - Laws of Mechanics - Vectors - Vectorial

representation of forces and moments - Vector operations.

UNIT 2: Statics of particles 06

Coplanar Forces - Resolution and Composition of forces - Equilibrium of a

particle - Forces in space - Equilibrium of a particle in space - Equivalent systems

of forces - Principle of transmissibility - single equivalent force.

UNIT 3: Equilibrium of rigid bodies 06Free body diagram - Types of supports and their reactions - requirements of stable

equilibrium - Equilibrium of rigid bodies in two dimensions - Equilibrium of rigid

bodies in three dimensions.

UNIT 4: Properties of surfaces and solids 08

Determination of Areas and Volumes - First moment of area and the centroid -

second and product moments of plane area - Parallel axis theorems and

perpendicular axis theorems - Polar moment of inertia Principal moments of

inertia of plane areas - Principal axes of inertia - Mass moment of inertia - relation

to area moments of inertia.

UNIT 5: Friction 03

Frictional Force - Laws of Coloumb friction - Simple Contact friction - Rolling

Resistance - Belt Friction.

UNIT 6: Dynamics of particles 08

Displacement, Velocity and acceleration their relationship - Relative motion -

Curvilinear motion - Newton's Law - Work Energy Equation of particles -

Impulse and Momentum - Impact of elastic bodies.


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UNIT 7: Elements of rigid body dynamics 06

Translation and Rotation of Rigid Bodies - Velocity and acceleration - General

Plane motion - Moment of Momentum Equations - Rotation of rigid Body - Work

energy equation.

Recommended Books:

1. Beer and Johnson, " Vector Mechanics for Engineers ", Vol. 1 " Statics " and

Vol. 2 " Dynamics ", McGraw Hill International Edition, 1995.

2. Merriam, " Engineering Mechanics ", Vol.1 " Statics " and Vol.2 " Dynamics 2/e

",Wiley International, 1988.

3. Rajasekaran S. and Sankara Subramanian, G., " Engineering Mechanics - Statics

and Dynamics ".

4. Irving, H., Shames, " Engineering Mechanics - Statics and Dynamics ", Thrid

Edition, Prentice-Hall of India Pvt.Ltd., 1993.

5. Mokoshi, V.S., " Engineering Mechanics ", Vol.1 " Statics " and Vol.2 "

Dynamics ", Tata McGraw Hill Books, 1996.

6. Timoshenko and Young, " Engineering Mechanics ", 4/e, McGraw Hill, 1995.

7. McLean, " Engineering Mechancis ", 3/e, SCHAUM Series, 1995.


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BBT-104 ECOLOGY AND BIODIVERSITY 40hrs

UNIT 1:Introduction and the science of biology 10

Characteristics of living organisms, the biological hierarchy, organizing concepts

and common themes in biology, Size and time scales, Evolutionary biology,

Darwin and descent with modification- Natural selection, Macro-evolution, the

origin of species, The fossil record,. Phylogeny and systematic, tree of life

UNIT 2:The evolution of diversity 12

Prokaryotes - diversity and ecological impact, The origin of eukaryotic diversity

Protists, algae and fungi with emphasis on their evolution and symbiosis, role of

fungi in colonization of land by plants, role of soil microbiota in plant protection

and ecosystem restoration, Predators-microbial food webs and their interactions

with metazoans, Plant evolution- adapting to life on land, Vascular plants with

and without flowers, Fungi - diversity and ecological impact, Animal evolution-

Phylogeny and diversity, Invertebrates- pathogenicity and drug resistance and

evolutionary significance, Vertebrates: Evolution and diversity, The scope of

ecology. Energy and nutrients flows. Ecological interactions and biocomplexity.

Communities and ecosystems

UNIT 3: Molecular ecology 10

Molecular markers in population and systematic biology, genetic identity at the

molecular level, paternity analysis, genetic structure of populations,

phylogeography, natural hybridization, conservation genetics

UNIT 4: Evolutionary ecology 8

Planning and conducting of experimental studies, data analysis, writing research

report and its, Evaluation: Population ecology, Community ecology, Ecosystem

ecology.

Recommended Books

1. Environmental Science- Towards a suistanable Future; Richard T. Wright, benard

J. Nebel; Prentice, Hall Indi, 9th

Edition (2004).


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2. Ecology Principles of Applications; J. L. Chapman, M. J. Reiss, Cambridge

Press University, 2nd

Edition (1999).

3. Ecology Theories & Application; Peter Stiling; Prentice, Hall India, 4th Edition

(2001)

4. Biological Science; D. J. Taylor, n. P. P. Grcen, G. W. Stout; Cambridge Press

University, 3rd

Edition (2003).

5. Biology; Neil Campbell & Jane Reece; Benjamin Cummings, 7 th Edition (2004).

6. A Textbook on Biotechnology; H. D. Kumar; Affiliated East West press Private

Ltd, 2nd

Edition (2003).

7. From DNA to Diversity- Sean B. Carroll, Jennifer K. Grenier, Scott D. Weatherbee.

8. Animal diversity by Earl D. Hanson

9. Biodiversity - Kevin J. Gaston, John I. Spicer, Private Ltd, 2nd Edition.

10.Origin of species by means of natural selection - Mark Ridley,Don E. Wilson


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BBT - 105 APPLIED PHYSICS 40hrs

UNIT 1:Acoustics and ultrasonics 07

Classification of sound, Properties of sound, Factors affecting acoustics of

building, Ultrasonic production-Magnetostriction & Piezoelectric methods,

Detection of ultrasonic sound, Applications-SONAR, Velocity of blood flow,

movement of heart

UNIT 2:Oscillations and waves 06

Simple Harmonic motion, Damped and forced oscillations, LCR circuit,

Resonance, Superposition

UNIT 3: Wave Optics 06

Interference, Diffraction, Polarization

UNIT 4: Electrostatics 06

Vector field, Electric flux, Statements of Gausss divergence and Stokes theorem

UNIT 5: Laser and Fibre optics 07

Laser production-principle and examples, Light emitting diodes, Semiconductors,

Optical fibres, Applications

UNIT 6: Semiconducting materials 08

Origin of gap bands in solids, n-type and p-type semiconductors, Compound

Semiconductors, Light Emitting diodes, Superconductivity

Recommended Books:

1. Waves and Oscillations: A.P.French.

2. Physics of Waves:W.C.Elmore & M.A.Heald.

3. Introduction to Electrodynamics: D.J.Griffith

4. Electrodynamics:Gupta,Kumar & Singh

5. Optics by A.K.Ghatak

6. Engineering Physics by Satya Prakash.


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BBT 106 APPLIED CHEMISTRY 40hrs

UNIT 1: Atomic Structure 04

Quantum Mechanical Model of Atom, Schrodinger Wave equation for Hydrogen

Atom, Atomic orbitals and their energy, Structure of ofMultielectron atoms

UNIT 2: Chemical Bonding 06

Valence Bond Theory, Molecular Orbital Theory, Linear Combination of Atomic

orbitals, Comparative study of VB and MOT, Metallic Bond

UNIT 3: Chemical Kinetics 08

Average and instantaneous rate of a reaction, Integrated rate expressions of zero

and first order reactions and their derivations, Half life and time constants,

Determination of rate constant/ order of reaction (Graphical Method and Ostwald

Isolation method), Temperature dependence of rate constant- Arrhenius equation,

Mechanism of reaction- Elementary and complex reaction

Effect of solvents (aqueous and nonaqueous), pH, pressure, salts (osmatic) on

chemical reactions, Balance of chemical reactions and factors influencing reaction

equilibrium.

UNIT 4: Surface Chemistry 06

Adsorption-physical and chemical, Factors affecting adsorption-Pressure and

Temperature, Colloids-Definition, types and classification, Applications of

surface chemistry-, Enzyme Catalysis-surface reaction kinetics, Multimolecular,

macromolecular and associated colloids (micelles)

UNIT 5: Thermodynamics 06

Laws of Thermodynamics, Entropy, Gibbs Free Energy, Standard entropies and

free energy of formation, Free energy change and chemical equilibrium


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UNIT 6: Stereochemistry 07

Geometrical isomerism, conformation and configuration, Optical activity and

specific rotation, Chirality-chiral centre, Enantiomers, Racemisation,

Diastereomers, Importance of Stereochemistry

UNIT 7:Contemporary Chemistry 03

Chemistry and warfare, Chemistry and new materials

Recommended Books:

1.Textbook of Physical Chemistry:P.L.Soni, O.P.Dharmarha,U.N.Dash.Sultan Chand &

Sons,First Edition(2006).

2.Adventures in Chemistry:Julie T.Millard.Houghton Mifflin Company.First

Edition(2008).

3.Organic Chemistry:R.T.Morrison,R.N.Boyd.Pearson Education.Sixth Edition(2003)

4.Physical Chemistry:Peter Atkins,Julio De Paula.Eighth edition(2006)


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Practical I:

1. Study of Microscope

2. Mitosis in an onion root tip.

3. Metaphase arrest and chromosome analysis

4. Meiosis in Tradenscantia pollen grains

5. Giemsa staining

6. Histochemical localization of DNA and RNA in onion peel.

7. Histochemical localization of mitochondria - Jannus Green stain.

8. Study of cell inclusion bodies and starch granules

9. Study of plasmodesmata

10.Study ofParamecia /Cyanobacteria cell with heterocyst


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Practical II:

1. To determine the specific rotation of sugar by Biquartz or Laurent half shade

polarimeter

2. To determine the width of a narrow slit using diffraction.

3. To determine the wavelength of sodium light by Newtons ring method.

4. To determine the value of g using bar pendulum.

5. Determinaton of surface tension in Liquid

6. Application of surface tension in mixture analysis

7. Application of distribution law in the determination of equilibrium constant

8. Plant pigment separation by paper chromatography

9. Practicals of Engineering Mechanics10.Practicals of Engineering Mechanics


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BBT - 201 CONCEPTS OF BIOTECHNOLOGY 40hrs

UNIT 1: Introduction to biotechnology 03

Biotechnology as an interdisciplinary science, Historical developments

UNIT 2: Industrial biotechnology 08

Fermentation techniques (solid state, submerged), Generalized design of

Bioreactor (stirred tank), Downstream processing an over view.

UNIT 3: Enzyme biotechnology 08

Enzymes - an Introduction, Immobilization of enzymes.(methods, effects,

advantages and disadvantages), Applications of enzymes. (Analytical uses,

industrial uses), Enzyme therapy, Biosensors- an over view, Biochips and

bioelectrical devices.

UNIT 4: Fuel and Material biotechnology 05

Biofuels an introduction, Biogas production, Bioethanol production, Tanning of

leather, Biodeterioration and preservation of food and textiles.

UNIT 5: Products and services based on biotechnology 08

Beer production, Citric acid production, Micro propagation an over view,

Protoplast fusion, Somatic cell fusion, Hybridoma technology, Polymerase chain

reaction and its applications, Vaccines.

UNIT 6: Environmental biotechnology 08

Biodegradation of xenobiotic compounds, Biosurfactants, Microbial enhanced oil

recovery, Soil remediation, Bio control agents.

Recommended Books:

1. Biotechnology expanding horizons; B.D. Singh; Kalyani publishers; 2004.2. Enzyme biotechnology; G.Tripath; A.B.D. publishers; 2003.


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3. Enzymes- Biochemistry, biotechnology, clinical chemistry; Trevor Palmer;Harwood publishing Ltd; 2004.

4. Environmental Biotechnology; M.H. Fulekar; Oxford and I.B.H publishing Co.Pvt. Ltd.

5. An introduction to gene cloning; T.A.Brown; Oxford publications; third edition.6. Advances in biotechnology; S.N.Jogdand; Himalaya publishing house.


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BBT 202 GENETICS AND EVOLUTION 40hrs

UNIT 1: History and Disciplines 01

A brief overview of the History of Genetics, An outline about the various

disciplines in Genetics.

UNIT 2: Classical Genetics 10

Mendelian Genetics: Mendels Law of inheritance, Chromosome theory of

hereditary, Linkage and crossing over, Allelic variation, Multiple alleles, Gene

interaction.---Nonmendelian inheritance: Maternal effects, cytoplasmic

inheritance. ---

-----------

UNIT 3: Population genetics 04

Allelic and genotypic frequencies, Hardy-Weinberg Equilibrium, Changes in

allelic frequencies, Inbreeding and out breeding.--

---------------------------------------

UNIT 4: Mechanism of Sex determination 04

Factors influencing sex determination, Dosage compensation, Sex limited and Sex

influenced traits--------------------------

UNIT 5: Evolution 07

Theories of Origin of life, Classical experiments, Theories of Evolution,

Evidences for evolution, Molecular evolution, Evolution and speciation-------------

--------------------------------------

UNIT 6: Techniques 10

Karyotyping and Banding techniques, Physical and genetic mapping, Tetrad

analysis and somatic cell hybridization, Pedigree analysis and genetic counseling,

Population statistics.

-----------------------------------------------------

UNIT7: Genetics and social Issue 04

Human Genome Project,-Eugenics,-Sociobiology


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Recommended books :

1. Gardner EJ, Simmons MJ, Snustard DP,(2004).Principles of Genetics.8th

Ed,John

Wily & sons Inc

2. Jain H K, (2004), Genetics: Principles, Concepts & Implications. Oxford & IBH

Publishing Co.P.Ltd

2. Robert.R.H,(2002),Principles of Genetics,7th Ed.Tata McGraw Hill Publishing

Co.Ltd

4. Sambamurthy AVSS, (1999).Genetics.Narosa Publishing House

5. Winter P C, Hickey G I, Fletcher H L, (2003).Instant notes of Genetics, 2nd

Ed, Viva Books Pvt Ltd

6. Verma PS, Agarwal VK.(2007).Cell Biology,Genetics,Molecular

Bioloy,Evolution and Ecology.S.Chand


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BBT-203 ENGINEERING DRAWING 45 hours

Unit 1: Principles Of Graphics 10hrs

Two dimensional geometrical construction - Conic sections, involutes and cycloids -Representation of threedimensional objects - Principles of projections - standard codes of principles.

Unit 2: Orthographic Projections 26hrs

Projections of points, straight line and planes - ' Auxiliary projections '- Projection and

sectioning of solids -Intersection of surfaces - Development of surfaces.

Unit 3: Pictorial Projections 5hrs

Isometric projections - ' Perspectives '- Free hand sketching.

Unit 4: Computer Graphics 4hrs

Hardware - Display technology - Software - Introduction to drafting software.

TEXT BOOKS:

1. Narayanan, K.L., and Kannaiah, P., " Engineering Graphics ", Tata McGraw-HillPublishers Co., Ltd., 1992.

REFERENCES:

1. William M. Neumann and Robert F.Sproul, " Principles of Computer Graphics",McGraw Hill, 1989.

2. Warren J. Luzzadder and John M. Duff, " Fundamentals of Engineering Drawing",Prentice-Hall of India Private Ltd., Eastern Economy Edition, 1995.

3. Natarajan K.V., " A Text Book of Engineering Drawing ", Private Publication,Madras, 1990.

4. Mathur, M.L. and Vaishwanar, R.S., " Engineering Drawing and Graphics ", JainBrothers,New Delhi, 1993.


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BBT-204: DEVELOPMENTAL BIOLOGY 40hrs

UNIT 1: Introduction to the Embryology: 05

Ontogenic development as the subject matter of embryology, Phases of

ontogenic development, Trends of embryology: Descriptive, Comparative,

Experimental, Analytical

UNIT 2: Frog Embryology 05

Cleavage, Blastula, Gastrula: Dorsal lip of blastopore, Organizer (Chemical

composition)

UNIT 3: Mammalian Embrology: 20

Differentiation of Sex, Mechanism of fertilization. Cleavage pattern: Peculiarities

of cell division in cleavages, Chemical changes during cleavage, Patterns of

cleavage, Morula and Blastula, Gastrulation. Implantation:- Types of

Implantation, Biochemical and Physical aspects of Implantation, Endocrine

Mechanism of Implantation, Relation between the embryo and the maternal body

in mammals. Placentation in Mammals:- Development of Placenta, Types of

Placenta, Physiology of placenta

UNIT 4:Experimental Embryology 10

Multiple Ovulation, IVF in human, Cryopreservation of Human sperms, ova and

embryo.

Recommended Books:

Balinsky, B. I. (1975)An Introduction to Embryology, 4th ed. W. B. Saunders,

pub. Philadelphia


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Browder, L. W., C. Erickson and W.R. Jeffrey (1991)Developmental Biology,

3rd ed. Saunders College Publishing, Philadelphia.

Patten, B. M. and B.M. Carlson (1974) Foundations of Embryology, 3rd ed.

McGraw Hill, pub. New York.

Wolpert, L. et al., (2001) Principles of Development 2nd ed., Oxford U. Press.

Development Biology, T Subramoniam, Narvosa Publication 1st Edition

(2002)

Development at Biology, S.F. Gilbert, Sinawer Ass. Inc.

Human Embryology , Churchill.


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BBT- 205 ELECTRONIC ENGINEERING 40hrs

UNIT 1: Semiconductor Materials and Diodes 10

Review of Semiconductor Materials and Properties, The PN Junction, Introduction to

Semiconductor Diode Theory Diode Circuits: DC Analysis and Models, AC Equivalent

Circuits, other Diode Types Solar Cell, Photodiode, Light Emitting Diode, Schottky

Barrier Diode, Zener Diode, Temperature Effects, Understanding Manufacturers

Specifications.

UNIT 2: Diode Circuits 10Design of Rectifier Circuits, Half Wave Rectification, Full Wave Rectification, Filter,

Ripple Voltage and Diode Current, Voltage Doubler Circuit, Zener Diode Circuits,

Clipper and Clamper Circuits, Multiple Diode Circuits, photodiode and LED Circuits.

UNIT 3: The Bipolar Junction Transistor 10

Basic Bipolar Junction Transistor, Transistor Structures, NPN Transistor : Forward-

active mode Operation, PNP Transistor : Forward active Mode Operation, Circuits

Symbols and Conventions, Current Voltage Characteristics, Non ideal Transistor

Leakage Current and Breakdown, DC Analysis of Transistor Circuits, Common- Emitter

Circuits, Load Line and Modes Of Operation, Common Bipolar Circuits: DC Analysis,

Basic Transistor Applications Switch, Amplifer, Bipolar Biasing and Bias Stability,

Integrated Circuit Biasing, Multistage Circuits.

UNIT 4:The Field Effect Transistor 10

Junction Field- Effect Transistor, MOS Field- Effect Transistor, MOSFET DC Circuit

Analysis, Basic MOSFET Applications: Switch, Digital Logic Gate and Amplifier.

Temperature effects in MOSFETs, Input Protection in MOSFET. The power FET

(VMOS).


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Recommended Books:

Donald Schilling & Charles Belove, Electronic Circuits Discrete and Integrated, Third

edition, McGraw Hill International edition, 1989.


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BBT 206 BIOSTATISTICS 40hrs

UNIT 1: Data Presentation 03

Graphs: Histogram, Bar Charts: Simple and Multiple, Pie Chart , Problems and

Interpretations of each.Tables: Simple Table; Two Way and Multiway Table ;

Problems elaborating each.

UNIT 2: Measure of Central Tendency, Dispersion, Skewnwss and Kurtosis 06

Measure of Central Tendency: Arithmetic Mean, Median, Mode. Measure of

Dispersion: Variance, standard Deviation, Standard error, Coefficient of

variation. Skewness: Discussion, Karl Pearsons co-efficient of Skewness.

Kurtosis: Discussion. Note: Discussion of uses and interpretations is expected.

UNIT3: Exact Sampling Distribution I 06

Chi Square Test. Definition: Applications of Chi Square Test. Chi Square Test for

population variance. Chi Square Test for Goodness of Fit. Chi Square Test for

independence of attributes (2 x 2 contingency table only). Yates Correction

method. ( Problems on Chi-Square with Yates correction need to be covered in

practical session). Note on degrees of freedom. Problems on each.

UNIT4: Exact Sampling Distribution II 14

Parametric Test: t-test. Definition: Simple t- Test for mean and variance.

Independent t-Test for Mean and Variance. Paired t- Test for mean and variance.

Problems on each. Z-test. Definition: Z- Test for significance of difference for

sample mean and Population Mean. Z- Test for significance of difference for

sample variance and Population Variance. Z- Test for significance of difference

for sample means of two group. Z- Test for significance of difference for

sample variance of two group. Problems on each. ANOVA, One Way

ANOVA, Two Way ANOVA, Problems on each. Non Parametric Test: Test of

Randomness, Median Test, Sign Test, Mann-Whitney-Wilcoxon U Test,

Problems


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UNIT 5: Testing of Hypotheses 05

Introduction: Assumption of Parametric test, General Hypotheses, Statistical

Hypotheses, Simple and Composite Hypotheses, Null Hypotheses, Alternative

Hypotheses, Critical Region, Two Types of Error, Level of Significance, Steps in

Solving Testing of Hypotheses problem.

UNIT 6: Correlation and Regression 06

Definition of Correlation for Bivariate Distribution. Types of Correlations,

Positive Correlation, Negative Correlation, No Correlation. Methods for

Identifying Types and Degree of correlation, Scatter Diagram, Karl Pearsons

Correlation Coefficient, Problems , Interpretations of Results, Rank Correlation,

Limitations of correlations, Regression for Bivariate Distribution, Line of

regression, Regression Curves, Regression Coefficients, Properties of regression

Coefficients.

Recommended Books:

1. Fundamentals of mathematical Statistics.

2. Fundamentals of Applied l Statistics.

3. Statistical Method.

4. Probability and Random Process


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Practical - I

1. Dissection of salivary glands

2. Preparation of polytene chromosomes from salivary glands ofChiromonas/

Drosophila/Mosquito

3. Induction of polyploidy in onion root tips

4. Study of simple and complex tissue by maceration

5. Abnormal secondary growth in carrot and Bignonia

6. Study of permanent slides:

- T. S. of dicot stem

- T. S. of monocot stem

7. Study of permanent slides:

- Types of ovule

- T. S. of anther

- T. S. of ovule

- Embryo sac

8. Pollen viability test

9. Study of Barr body from buccal epithelium

10. Exercises on population genetics and linkage


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Practical - II

1. Creating Charts with Excel and /or SPSS for different types of DATA.

2. Creating Tables with Excel and /or SPSS for different types of DATA.

3. Calculation of Mean, Median and Mode with excel and SPSS.

4. Calculation of Variance, Coefficient of variation and Skewness and Kurtosis withexcel and SPSS.

5. Chi Square Test with Excel and/or SPSS

6. Correlation Analysis with Excel and/or SPSS.

7. Regression Analysis with Excel and/or SPSS

8. Fitting of Regression Line with Excel and/or SPSS.

9. Analyzing data for Normality assumption.

10.t- test with Excel and/or SPSS.

11.ANOVA with Excel and/or SPSS .

12.Analysis of Non Parametric data with Non Parametric tests with Excel and/orSPSS.

13.Study of diode characteristics

14.Study of clipper and clamper circuit.

15.Study transistor characteristics (i.e. input, output characteristics of CEconfiguration


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BBT - 301 MICROBIOLOGY 40hrs

UNIT 1: History of microbiology : 03

Landmark discoveries in microbiology, Scope of microbiology

UNIT 2: Microscopic observation of micro-organisms 04

Microscopy Light microscope and its parts, Principle behind staining of micro-

organisms

UNIT 3: Classification of micro-organisms 04

Difference between prokaryotic and eukaryotic cell, different methods of

classification of micro-organisms

UNIT 4: The morphology and ultra-structure of bacteria: 10

Pure culture techniques. Nutritional requirements of bacteria and methods of

cultivation, Growth of bacteria, Growth cycle, Measurement of bacterial growth.

Differentiation pattern among prokaryotes

UNIT 5: Control of micro-organisms using physical and chemical agents 06

UNIT 6: Microbial diversity 10

Photosynthetic microorganisms, Chemolithotrophs, Gliding bacteria, Budding and

appendaged bacteria, Archaebacteria -Archae as earliest life forms, introductionto thermophiles, psychrophiles, halophiles, alkalophiles, acidophiles,

hyperthermophiles, Introduction to viruses, fungi and protozoa.

UNIT 7: Microbial Genetics 03

Methods of gene transfer: Bacterial transformation, conjugation and transduction.

Recommended Books:

1. Microbiology; Michael J. Pelczar, E. C. S. Chan, Noel R. Krieg;;Tata Mc Graw

Hills; 5th

edition (1993)


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2. Microbiology; Lansing M Prescott, John P.Harley, Donald .A.Klein; McGraw

Hills 5th

edition (2005)

3. General Microbiology; Roger Y. Stanier, John L. Ingraham, Mark L. Wheelis,

Page R Painter; MacMillan Press ltd; 5th

edition (1986)

4. Microbiology A.J.Salle

5. Microbiology, Tortora, Funke and Chase, Benzamin & Cummings


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BBT 302 MOLECULES OF LIFE 40hrs

UNIT 1: Nature of biological materials 04

Introduction to biochemistry, importance, general properties of organic

molecules, functional groups, suitability of organic molecules for generation of

structures. Water-Structure, properties and physiological importance.

UNIT 2: Carbohydrates 08

Classification, structures, properties and functions of monosaccharides,

disaccharides (lactose, maltose & sucrose) ,polysaccharides (starch, glycogen,

cellulose, pectin, dextrin, chitin, heparin, cell wall polysaccharides),biological

importance of carbohydrates.

UNIT 3: Lipids 08

Classification, nomenclature, structure of fatty acids. Chemical properties of fats-

saponification number, iodine number, acid number,rancidity,RM

number.Chemical properties and functions of of phospholipids-lecithin,cephalin,

phosphotidyl serine, phosphotidyl ethanolamine , glycolipids, isoprenoids and

sterols(cholesterol).Biological importance of lipids.

UNIT 4: Proteins 08

Amino acids-classification based on polarity, structure and properties, essential

and nonessential. Classification of proteins (ASBC and APS),functional

classification, peptide bond. Protein structure-primary, secondary ,tertiary and

quaternary, forces stabilizing protein structure, protein denaturation, biological

importance.

UNIT 5: Nucleic acids 06

Structure of purines ,pyrimidines, nucleosides, nucleoides. Properties of nucleic

acids(denaturation, annealing, buoyant density),Structure of DNA, forms of

DNA(A,B,Z)Structure and function of different RNAs (m,r,t), biological role.


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UNIT6: Vitamins and minerals 06

Fat soluble vitamins(A,D,E,K) and water soluble(B complex series and Vit. C)-

sources, RDA, role, deficiency disorders(no structures). Minerals-

Macro(Na,P,K,S,Ca,Mg &Cl), Micro(Fe, I, Cu, Zn, Mn,Co, Mo, Se, Cr, F)-

sources and role.

Recommended Books

1.Practical Biochemistry-Plummer,D,3rd

Edn.,Tata McGraw Hill publishing

Co.,1988

2.Laboratory Manual of Biochemistry-Jayaraman J,New age international

publication,1981.

3. Practical Biochemisty-Swahney, Singh, Narosa Publication,2005.

4. Practical Clinical Biochemistry-Varley;H,CBS publications.


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BBT - 303 BIOLOGICAL AND BIOCHEMICAL TECHNIQUES 40hrs

UNIT 1: Solutions and buffers 05

Nature and preparation of buffers, Determination of pH, Instrumentation-pH

meter, Applications of pH measurement

UNIT 2: Centrifugation 05

Principle and instrumentation, Types of centrifugation, Sedimentation coefficient,

Applications

UNIT 3: Chromatography 07

Principles and instrumentation, Types-Paper, Ion-exchange, affinity, Applications

UNIT 4: Electrophoresis 07

Principles and instrumentation, Types of electrophoresis-Free & Zone, Paper and

Gel electrophoresis, Immunoelectrophoresis, Applications of electrophoresis

technique

UNIT 5: Radioisotope technique 05

Nature of radioactivity, Detection and measurement, Advantages and limitations

of radiotracer experiments, Applications of radioisotope in biological sciences,

Safety aspects

UNIT 6: Polarimetry 06

Polarization of Light, Plane of polarization, Optical activity, ORD and CD effects,

Instrumentation, Applications of CD and ORD

UNIT 7: Spectroscopy 05

Region and properties of EM radiations, Principles, photoelectric effect,


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absorptionspectrum, emission spectrum, Instrumentation, Types of absorption

spectroscopy (overview), Types of absorption spectroscopy, Applications of

absorption spectroscopy.

Recommended Books

1. Instrumental methods of chemical analysis.B.K. Sharma, Goel Publishing House, 25th

edition

2. Principles and techniques of biochemistry and molecular biology, Wilson and Walker,

Cambridge University Press, 6th

edition

3. Instrumental methods of chemical analysis, Chatwal and Anand, Himalaya Publishing

House, 5th

Edition

4. Tools and techniques of biotechnology, Mousumi Debnath, Pointer Publishers, 1st

edition

5. Biophysical chemistry-Principles and techniques, Upadhyay; Upadhyay and Nath, H

Himalaya Publishing House, 3rd

Edition

6. Physical biochemistry- applications to biochemistry and molecular biology, David

Freifelder, Freeman and Co., 2nd

edition.


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UNIT 6: Respiratory system 06

External and internal anatomy of: Nose, Pharynx, Trachea, Lungs and pleurae.

Recommended Books:

1. Text Book of Medical Physiology; Arthur Guyton and John Hall;; Elsevier, Reed

Elsevier, India Pvt. Ltd. 10th

Edition (2000).

1. Principles of Anatomy and Physiology; Tortora Gerard J. Grabowski; John Wiley

and Sons Incubator. New Jersey,10th

Edition; (2003).

2. Human Anatomy and Physiology; Elaine N. Marieb;; Benjamin/ Cummings

Science Publishing, California, 4th

Edition (2004).

3. Histology; W.A. Ham,Philadelphia& Montream, J.B. Lippincott comp.,5th

Edition. (1965)

4. Concise Histology; Bloom and Flawcett, Ronald P. Jensh , Arnold Publication,2nd

Edition (2002)

5. Textbook of Histology; Bloom and Flawcett, Chapman & Hall , 12th Edition

(1994)


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BBT - 305 MAMMALIAN PHYSIOLOGY 40hrs

UNIT1:Physiology of muscle contraction and nerve impulse transmission 06

UNIT 2: Excretion 08

Types of body fluids and maintenance of osmotic equilibrium between

extracellular and intracellular fluids, Formation of urine by kidney: Glomerular

filtration, tubular reabsorption and tubular secretion.

UNIT3: Circulation 10

Biophysics of blood flow, haemodynamics, capillary dynamics and exchange of

fluid between the blood and interstitial fluid, lymphatic system, Blood pressure,

measurement of blood pressure, cardiac output and venous return, Pulmonary

circulation, systemic circulation, coronary circulation, blood flow through specific

areas of the body.

UNIT 4: Reproduction 08

Spermatogenesis, Hormonal regulation of spermatogenesis, Testosterone and its

functions, Oogenesis, Menstrual cycle, Hormonal regulation of oogenesis and

menstrual cycle, Oestrogen, progesterone and their functions.

UNIT 5: Respiration 08

Pulmonary ventilation: Inspiration and Expiration, Physical principles of gaseous

exchange, diffusion of O2 and CO2 between the alveoli and the blood, Transport

of O2 and CO2 in the blood and body fluids.

Books recommended

1. Text Book of Medical Physiology; Arthur Guyton and John Hall; Elsevier, Reed Pvt.

Ltd. 10th

Edition; (2000).

2.Human Physiology Volume Elsevier, India I and II; C.C. Chatterjee; Medical Allied

Agency 10th

Edition;(2004).


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3. Human anatomy and Physiology; Elaine N. Marieb; Pearson, Benzamin cummings,6th

edition(2004).

4. Principles of Anatomy and Physiology; Tortora Gerard J. Grabowski; John Wiley and

sons .New Jersey, 10th

edition (2003).

5. Fundamentals of Anatomy and Physiology; Fredric H. Martini, Prentice Hall

international editions, 7th

edition (2006).

6. Anatomy and Physiology ,Thibodeau ,Patton ,Mosby publications ,Third edition

(1996).


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BBT 306 BIOPHYSICS 40 hrs

UNIT 1: Biophysical Principles 10

Diffusion: Principles and mechanism, Diffusion across membranes. Osmosis:

Principles and mechanism, Osmoregulation in plants. surface tension: Principle

and mechanism, Biological importance in living cells and human body.

Ultrafiltration: Principle & mechanism, Biological importance in body.

Hydrophobic effect

UNIT 2: Biological Macromolecules 15

General principles: Configuration & Conformation, Interaction of biomolecules

with water, Stabilising interaction. The structure of proteins: Helical symmetry, H

bonding in secondary structures. Effect of peptide bond on protein conformation,

Ramachandran plot. The structure of nucleic acid: Helical structure of Polynucleic

acid, Base pairing, base stacking and torsional angles.

UNIT 3: Thermodynamics & Biophysics 10

Laws of thermodynamics and living organisms, Living body as a thermodynamic

system, Biosphere as a thermodynamic system, Evolution as a thermodynamic

process

UNIT 4: Membrane Biophysics 05

Membrane Structure: Lipid bilayer, Micelle, Physico chemical properties of

membrane, Modification of cell membrane and change in biophysical properties,

Conformational/Dynamic properties of membranes

Recommended Books

1.A textbook of biophysics, R.N.Roy, New Central Publication, 1st

edition.

2. Elementary biophysics. P.K.Srivastava Narosa Publication, 1st

edition.

3. Biophysical Chemistry.Upadhyay & Nath, Himalaya publications 3rd

edition.


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4. Biological thermodynamics.Donald T.Haynie,Cambridge University Press, 1st

edition.

5.Principles of Physical Biochemistry.Kensl E.van Holde, W.Curtis Johnson, P.Shing Ho,

Pearson Prentice Hall,2nd

edition.

6. Biophysical chemistry Part I: The conformation of biological macromolecules.

Biophysical chemistry Part III: The behavior of biological macromolecules.Cantor and

Schimmel, W.H.Freeman and Company, 10th

edition.


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Practical - I

1. Preparations of solutions molar,normal,ppm,percent.

2. pH meter and preparation of buffers.

3. Qualitative tests for

a) Proteins-ninhydrin,biuret

b) Carbohydrates-Molischs, Benedicts, Fehlings, Bials, Barfoeds,

Iodine.

c) Lipids-solubility tests, Sudan III

4. Iodine value/ Saponification value of oils.

5. Determination ofmax and verification of Beer & Lamberts law

5. Estimation of maltose by DNSA method

6. Estimation of proteins by Biuret method

7. Separation of amino acids by paper chromatography

8. Separation of lipids by TLC

9. Isolation of starch from potato

- casein from milk

10. Separation of plant pigments by column chromatography


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Practical II

1. Staining Techniques-

a) Basic : Monochrome/ negative

b) Differential :- Gram stain, Spore, Capsule, Cell Wall

2. Aseptic transfer Techniques

a) Flask to flask

b) Tube to plate

c) Pipetting from Flask to tube/ Tube to tube

3. Preparation of microbial suspension in saline and serial dilution

4. Preparation of nutrient media- broth and agar ( Nutrient and Macconkeys)

5. Isolation of microorganism by- Streak plate, T method, Pentagonal Streaking

6. viable count of bacteria- Pour plate, Spread plate

7. Isolation of antibiotic producers: crowded plate, wilkins overlay

8. Routine analysis of blood-RBC count,Differential WBC count

9. Permanent slides of reproductive system- Spermatozoan, V.S. of testis, T.S. of -

seminiferous tubeles, ovary, uterus, pituitary gland, adrenal gland, thyroid

gland, parathyroid gland, pineal gland.

10.Observation of chart and models Respiratory system, Models of lymphatic

system


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BBT - 401 INTERMEDIARY METABOLISM I 40hrs

UNIT 1: Introduction to metabolism 06

Introduction to intermediary metabolism the basic metabolic pathways: Anabolic,

catabolic, amphibolic pathways. Approaches to biochemical investigations-

perfusion of isolated organs, slice technique, Tracer techniques and mutant studies

for elucidation of metabolic pathways.

Unit 2: Bioenergetics 08

Study of equilibrium in aqueous solution- concept of free energy, standard free

energy, endergonic, exergonic and coupled reactions, steady state and non

equilibrium systems. Nature and generation of phosphate bond energy. Energy

currencies of the cell. The ATP cycle, other high energy compounds. Redox

potentials and redox reactions,. Relation between reduction potentials and

frequency change. Donnan Membrane equilibrium Passive, Active and Facilitated

Transport. Examples H+, Cl- ,Na+ / K+ gradients, role of energy conversion

Mitchell hypothesis. Transformation of diffrerent energy forms; e.g. chemical and

mechanical. Efficiency of interconversion of various forms of energy.

UNIT 3: Biological Oxidation 08

The electron transport chain organization and role in electron capture, electron

transport complexes, oxidative phosphorylation, the chemiosmotic theory.

Mechanism of action of ATP Syntheses (FoF1 ATPase). Inhibitors of respiratory

chain and oxidative phosphorylation, uncouplers,ionospheres, mitochondrial

transport system, ATP/ ADP exchange malate/ glycerophosphate shuttle.

Unit 4: Photosynthesis 06

Chloroplast -Pigment Systems, Light harvesting complex, Hill Reaction, light

reaction- cyclic and non-cyclic photophosphorylation, Dark Reaction-Calvin

cycle, Hatch Slack pathway, CAM pathway, photorespiration.


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Unit 5: Carbohydrate metabolism & disorders 12

Glycolysis anaerobic, aerobic, pyruvate oxidation,TCA cycle,glycogen

metabolism Cori cycle, HMP Shunt, Gluconeogenesis, Glyoxalate Cycle,

Metabolisms of galactose and mannose. Role of Fructose 2,6 biphosphate,

regulation of glycolysis, gluconecogenesis and glycogen metabolism, pivotal role

of TCA cycle in metabolism. Disorders- Blood Sugar levels, Diabetes mellitus,

metabolic complications, glucose tolerance tests, glycosylated hemoglobin,

glycogen storage diseases, galactosemia, fructosemia, pentosemia,

mucopolysaccharidosis, ketone bodies.

Recommended Books:

1.Biochemistry- Stryer,Berg,6th Edition,W.H.Freeman and Co.,2007.

2.Biochemistry-Metzler;DE,2nd

Edn.,Academic press,2001.

3.Lehninger Principles of biochemistry-Nelson,Cox,4th

Edn., W.H.Freeman and

Co.,2005.

4 Biochemistry Voet;D,Voet;J,3rd

Edn.John Wiley and sonsInc.,2004.

5 Outlines of Biochemistry-Conn;E,Stumpf,5th Edn. ,Tata-McGraw Hill,1988.

6 Harpers Principles of Biochemistry-Murray,Gardener,Mayes,Rodwell,27th

Edn.McGraw Hill Education,206

7 Biochemistry- Rawn,D,Pamina publications,2004

8.Textbook of biochemistry-West,Todd,Mason,VanBrergen,4th

edn. Oxford&IBH,1966.

9.Biochemistry-Satyanarayan.U,Books &Allied(P) Ltd.,2003.

10. Biochemistry-Champe;P,3rd

Edn.,Lippincott Willams&Wilkins,2005.

11. Biochemistry-Zubay;G,3rdEdn.,Pearson Education P.Ltd,2003


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BBT - 403 MOLECULAR BIOLOGY OF PROKARYOTES 40 hrs

UNIT 1 : 05

Nucleic acids structure, Identification of DNA as genetic material, Types of DNA

(A, B and Z DNA), Types of RNA (mRNA, tRNA, rRNA).

UNIT 2: 04

Structure and organization of prokaryotic genome, Properties of DNA, Chemical

and physical properties, Spectroscopic and Thermal properties, DNA supercoiling

UNIT 3: 06

DNA recombination, Molecular mechanism and models of recombination,

Homologous recombination, Site-specific recombination, Transposition

UNIT 4: 04

Mutagens and mutagenic agents, DNA damage and repair

UNIT 5: 24

Central dogma of molecular biology: DNA replication, Strategies of replication,

Mechanism and the enzymes involved, Lagging and leading strands, Models of

replication (Theta, Rolling circle). Transcription: Mechanism and steps involved

(Initiation, Elongation and Termination), Regulation of transcription, Lac operon-

Positive and negative control, Trp operon- Repression, attenuation and

antitermination, Regulon, Viral strategies for transcription control. Translation:

Cell components involved (mRNA, tRNA, and rRNA), Ribosomes (structure and

composition), Genetic code and wobble hypothesis, Steps Involved (Initiation,

Elongation and Termination), Translational control and post translational

modifications.


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Recommended Books:

1) Molecular Biology; David Freifelder, Narosa Publishing House,2nd edition (2004)

2) Microbial Genetics; David Freifelder, Narosa Publishing House, 2nd

edition(2004)

3) Principles of Gene Manipulations; S. B. Primrose, R. M. Twyman, R. W. old,

Blackwell Science,6th

Edition (2003).

4) Gene VIII; Benjamin Lewin; Oxford Univ. Press ,8 th edition (2004)

5) Advanced Molecular Biology ;R. M. Twyman, 1st Edition , (2003)

6) Instant Notes on Molecular Biology; P.C. Turner, A. G. Mclennan, A. D. Bates &

M. R. H. White, 2nd

Edition (2002)


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BBT - 404: MOLECULAR BIOLOGY OF EUKARYOTES 40hrs

UNIT 1:Eukaryotic chromosomes 03

Structure, Banding patterns Specialized structures lampbrush and Polytene

chromosomes.

UNIT 2: Concept of chromatin 03

Heterochromatin and Euchromatin - properties and functions, Factors involved in

heterochromatin formation.

UNIT 3: Nucleosomesand higher order chromatin 03

Types and assembly of histone proteins, Formation of higher order chromatin,

Regulation of chromatin structure.

UNIT 4: Eukaryotic genome complexity 09

C value paradox, Cot analysis and coding potential, repetitive/satellite DNA ,

Gene clusters, pseudogenes, Short and Long Interspersed Elements(SINES &

LINES), alu families and their significance, split genes, introns/exons and

overlapping genes.

UNIT 5: Modification of DNA 03

Methylation of DNA and its role, Invitro modification of DNA.

UNIT 6: RNA processing 05

Maturation of untranslated RNAs, End-modification and methylation of mRNA,

RNA splicing, RNA editing, Post-processing regulation.


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UNIT 7: Catalytic RNA 04

Group-I Introns, Ribozymes, Group- II Introns, Viroids, RNA editing by guide

RNAs, Autocatalytic protein splicing

UNIT 8: Homologous recombination in Eukaryotes 03

Homologous recombination during meiosis, Mating type switching, Genetic

consequences

UNIT 9: Gene regulation in Eukaryotes 07

Role of Eukaryotic activators, Signal integration and combinatorial control,

Transcriptional regulators by signal transduction, Histone modifications and

DNA, Post-transcription initiation, Role of RNA

Recommended Books:

1) Molecular Biology; David Freifelder, Narosa Publishing House,2nd edition (2004)

2) Molecular Biology of the Gene; James Watson 5th Edition (2006) Pearsons

Education.

3) Gene IX; Benjamin Lewin; Oxford Univ. Press ,9th edition (2008)

4) Advanced Molecular Biology; R. M. Twyman, 1st Edition , (2003)

5) Instant Notes on Molecular Biology; P.C. Turner, A. G. Mclennan, A. D. Bates &

M. R. H. White, 2nd

Edition (2002)

6) Genetics; P.K.Gupta, Rastogi Publications, 2005.


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BBT - 405 ADVANCED ANALYTICAL TECHNIQUES 40hrs

UNIT 1: Atomic absorption spectrometry 10

Introduction to Spectrometric Methods, Introduction to Optical Atomic

Spectroscopy, Atomic Absorption and Fluorescence Spectroscopy, Atomic

Emission Spectroscopy, Atomic Mass Spectroscopy, Atomic X-Ray

UNIT 2: Molecular absorption spectrometry 12

Introduction to UV/Visible Molecular Spectroscopy with application, Molecular

Luminescence Spectroscopy, Introduction to Infrared Spectroscopy with

application, Raman Spectroscopy, NMR with applications such as MRI,

Molecular Mass Spectroscopy and X ray crystallography.

UNIT 3: Separation Techniques: Chromatography 07

Introduction to Chromatographic Separations, Gas Chromatography, HPTLC,

HPLC, GC-MS, LC-MS with applications

UNIT 4: Separation Techniques: Electrophoresis 06

Introduction to Electrophoretic separations, Acrylamide, Starch, Gelatin, agarose

gels, IEF, capillary isoelectrofocussing, 2D electrophoresis, pulse fiend

electrophoresis, Capillary Electrophoresis and Capillary Electrochromatography.

UNIT 5: Specialized techniques 05

MALDI-TOF-MS, Alternative methods for ionization such as SELDI, ESI, CI,

FAB etc MS-MS analysis( Tandom MS).

Recommended Books

1. Principles of Instrumental Analysis Skoog, Holler, Nieman Thomson

Brooks/Cole Publication.


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BBT 406 IMMUNOLOGY AND VIROLOGY 40 hrs

UNIT 1: Fundamentals of Immune System 05

Innate and adaptive immunity, Cyokines,Complement system, Organs andTissues of Immune System- Primary and, Secondary lymphoid organs,

Haematopoiesis, Cells of Immune system.

UNIT 2: Antigens and Immunoglobulins 05

Antigens-Definitions, Hapten, . Adjuvant, Chemical Nature, Types of Antigenic

determinants. Immunglobulins- General Structure, Classes of Immunoglobulin

and Isotypes, Functions, Affinity and Avidity

UNIT 3: Immune Response 08

Lymphocytes: T-Cells- Classes, Structure and organization of TCRs, B-Cells-

Cell surface Receptors. APCs and Interaction and Mounting of Immune Response

(Antigen presentation, processing Overview, overview of MHC class) T and

B cell activation overview. Overview of Auto immunity, Transplantation and

Hypersensitivity

UNIT 4: Techniques in Immunology 04

Precipitation Reactions/ Tests, Agglutination Reactions, ELISA, RIA , FAT,

Immunoprecipitation, Chemiluminiscence and Elisa spot assay.

UNIT 5: Introduction to virology 05

Distribution, Classification, Morphology and ultra structure of viruses,

Cultivation of viruses, Virus purification and assays, Concepts of viroids and

prions. Identification tools- Microscopic, Biochemical, CPE, serological test.

UNIT 6: Mode of propagation 05

Adsorption and penetration, Viral replication:Nucleic acid synthesis (origin,

priming, types of DNA and RNA replication), Protein synthesis, Assembly and

release of virion, Life cycle of DNA and RNA Viruses


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Recommended Books

1. Immunology; Richard A. Goldsby, Thomas J. Kindt, Barbara A. Osborne, Janis

Kuby; W.H. Freeman & Company, 5th

edition (2003)

2. Roitts Essential Immunology; Ivan M. Roitt, Peter J. Delves Blackwell Science

Ltd., 10th

Edition (2001)

3. An Introduction to Immunology; C.V. Rao; Narosa Publishing House,1 st Edition

(2004)

4. 4.Instant Notes in Immunology; P.M. Lydyard, A. Whelan, M.W. Fanger BIOS

Scientific Publishers Ltd, 1st

Edition (2003)

5. Textbook of Microbiology ;R. Ananthnarayan, C. K. J. Panicker, Orient Longman

, 6th Edition (2003)

6. Immunology: Introductory textbook;Nandini Shetty, New Age International pvt.

Ltd. 1st

Edition (2003)

7. Principles of Virology by SJ Flint, LW Enquist, RM Krug, VR Racaniello, AM

Skalka ASM Press Washington 1st

edition (2002)

8. An introduction to genetic engineering by ST Desmond and Nicholl Cambridge

University Press 2nd

edition(2004)

9. General Microbiology Vol. II by Powar and Daginawala Himalaya Publ. House

8th

edition (2004)


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Practical I

1. Estimation of glucose by O Toluidine method & Folin Wu method.

2. Estimation of Calcium by EDTA method.

3. Estimation of phosphorus by Friske-Subbarao method

4. Estimation of lactose by Coles ferricyanide method.

5. Tests for the presence of amylase, urease and catalase.

6. Assay of amylase- Determination of Km and Vmax

7. Effect of pH, temperature, substrate concentration and inhibitors on amylase

enzymes activity.

8. Electrophoretic separation of proteins from various sources: native PAGE, SDS

PAGE.

9. Immobilization of amylase and its assay

10. Isolation and separation of chloroplast by sucrose density gradient centrifugation


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Practical II

1. Determination of the ABO blood group of the given sample and Rh typing, cross

matching

2. Preparation of O and H antigen from Salmonella.

3. Qualitative widal test. (Dreyers technique)

4. Ag-Ab Interaction: by Oudins, ouctherlonys and okalayas immunodiffusion

method.

5. Ag-Ab Interaction: by Double Immunodiffusion. (2 well method)

6. Isolation and enumeration of Bacteriophage from sewage

7. Normal and diauxic growth curve of E. coli

8. Enumeration of genomic DNA from onion

9. Isolation of cheek DNA

10.Isolation of DNA from Blood


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BBT 501 MOLECULAR CELL SIGNALING 40hrs

UNIT 1 : Overview of cell signaling 06

Forms of cell signaling - Autocrine, Juxtacrine, Paracrine, Endocrine, Synaptic.

Signaling machinery and pathways - Concept of signal / ligand (agonist and

antagonist), Concept of receptor (extracellular / intracellular), Ligand

Receptor interaction, Pathway operatives / cascades, Signal amplification,

Regulation of inter and intra cellular signaling, Membrane anchoring

UNIT 2 : Cell cell interaction 04

Junctions - Tight, Anchoring, Communicating. Adhesion molecules -

Cadherins, Integrins, Selectins, Ig superfamily

UNIT 3 : Receptors and characterization 15

G-protein Structure, Mechanism of activation. Enzyme linked - Receptor

Guanylyl Cyclase, Receptor Tyrosine Kinase, Tyrosine Kinase Associated

Receptor, Receptor Tyrosine Phosphatases, Receptor Serine Threonine

Kinase, Hitidine Kinase ( Cell Adaptation). Steroids receptors, Channels and

pumps - Na K ATPase, ABC transporters, Secondary Transporters, ACHR,

Sodium, Potassium. Techniques of receptor characterization - Patch Clamp

Techniques, Hormone Binding Assay

UNIT 4 : Secondary Messengers 05

cAMP, cGMP, Phosphoinositides, Ca2+

, DAG, Other lipid messengers, Nitric

Oxide

UNIT 5 : Kinase Cascades 05

Division (MAPK), Differentiation (BOSS / Drosophila eye), Survival (PI3K),

Development (NF-KB)


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UNIT 6 : Inhibition of signal transduction pathways or targets of drugs 02

Cholera, Whooping Cough, Steroidal and Non-steroidal anti-inflammatory

drugs, Sidenafil citrate

UNIT 7 : Signaling in plants 03

Ethylene, Phytochrome, meristem differentiation.

Recommended books:

1. Alberts et al. (1994) Molecular Biology of the Cell (3/e) Garland Science

Publishing, UK

2. Alberts et al. (2002) Molecular Biology of the Cell (4/e) Garland Science

Publishing, UK

3. Helmreich (2005) The Biochemistry of cell signaling Oxford University Press,

India

4. Lehninger et al. (2004) Lehninger Principles of Biochemistry (4/e) WH Freeman

and Co., USA

5. Stryer et al, (2002) Biochemistry (5/e) WH Freeman and Co., USA


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BBT 502 INTERMEDIARY METABOLISM II 40hrs

UNIT 1: Lipid metabolism and disorders 14

Oxidation of saturated and unsaturated fatty acids, oxidation. role of a carnitine,

ketogenesis and utilization. Biosynthesis of fatty acids, Metabolism of

Triglycerides Phospholipids and Sphingolipids, cholesterol biosynthesis,

regulation, transport, excretion, reverse cholesterol transport, formation of bile

acids, lipoprotein metabolism. Disorders of lipid metabolism plasma lipids and

lipoproteins, abnormalities -hypo and hyper cholesterolemia, lipidosis, clinical

inter relationship of lipids. Clinical inter-relations ships of lipids (sphigolipidosis,

multiple sclerosis) ,lipoproteins and apolipopeoteins, Inherited metabolic lipid

disorders Taysachs disease, Niemannpicks disease,ganglisidosis ,hypo and

hyper lipo proteinemia.

UNIT 2: Protein Metabolism and disorders 14

General reactions transamination, deamination, decarboxylation, ammonia

formation urea cycle, metabolic breakdown of aminoacids to amphibolic

intermediates, conversion of aminoacids to specialized products serotonin,

aminobutyric acid,dopamine, epinephrine ,norepinephnine ,melanin ,creatinine

and creatine, biosynthesis of non-essential aminoacids, key role of glutamate

dehydrogenase in nitrogen metabolism. Disorders of protein metabolism

hperammonemia, phenylalaninemia, alkptonuria, phenylketonuria,

homocystinuria,cysteinemia,tyrosinemia and related disorders of aminoacidurias,

Hartnups disease.

UNIT 3: Metabolism of Nucleotides and disorders 06

Purine and pyrimidine nucleotide biosynthesis and catabolism regulation of

purine and pyrimidone nucleotide biosynthesis. Disorders of nucleotide

metabolism


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UNIT 4: Metabolism of porphyrins 03

Biosynthesis and Degradation of porphyrins, heme , bile pigment formation,

transport and excretion.Porphyrias.

UNIT 5: Integration of metabolism 03

Inter-relationship of carbohydrate, protein and fat metabolism role of acetyl

CoA. and TCA cycle in the inter-relationship, interconversion of major

foodstuffs. Regulation of intermediary metabolism .

Recommended Books

1.Biochemistry- Stryer,Berg,6th Edition,W.H.Freeman and Co.,2007.

2.Biochemistry-Metzler;DE,2nd

Edn.,Academic press,2001.

3.Lehninger Principles of biochemistry-Nelson,Cox,4th

Edn., W.H.Freeman and

Co.,2005.

4.Biochemistry Voet;D,Voet;J,3rd

Edn.John Wiley and sonsInc.,2004.

5 Outlines of Biochemistry-Conn;E,Stumpf,5th Edn. ,Tata-McGraw Hill,1988.

6 Harpers Principles of Biochemistry-Murray,Gardener,Mayes,Rodwell,27th

Edn.McGraw Hill Education,206

7 Biochemistry- Rawn,D,Pamina publications,2004

8.Textbook of biochemistry-West,Todd,Mason,VanBrergen,4th

edn. Oxford&IBH,1966.

9.Biochemistry-Satyanarayan.U,Books &Allied(P) Ltd.,2003.

10. Biochemistry-Champe;P,3rd

Edn.,Lippincott Willams&Wilkins,2005.

11. Biochemistry-Zubay;G,3rdEdn.,Pearson Education P.Ltd,2003


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BBT 503 COMMERCIAL ASPECTS OF BIOTECHNOLOGY 40hrs

UNIT 1: Food and Beverages 10

Dairy products- cheese (Swiss, Cheddar and Camembert),

Beverages Bear & wine, Citric Acid, Mushroom, SCP Spirulina and Yeast,

Glutamic Acid, Acetic Acid, Oriental fermented foods & beverages (Koji, Meso,

Soy sauce), Bakery products (Bread), Sweetners, Microbial polysac charides

(Xanthon, Gellan).

UNIT 2: Agricultural Biotechnology 08

Biofertilizers: Types, process of manufacture and applications,

Biopesticides: Bacterial, fungal and viral- production and applications,

Aerobic and anaerobic treatment of agricultural wastes,

Composting and Vermicomposting.

UNIT 3: Health related products 12

Vit. B12, Blood products- Factor VIII, Streptokinase, Anticoagulants, therapeutic

proteins- Human GH, Interferons, Interleukins, insulin, erythropoietin, Nerve

growth factor, synthetic Vaccines.

UNIT 4: Secondary Metabolites 04

Antibiotics:- Penicillin, streptomycin, actinomycin, cephalorphorin

UNIT 5: Biotransformation 04

Basic Concepts, Products , Steroids biotransformation.

UNIT 6: GMP , QC and Marketing aspects of biotechnology 02


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Recommended Books:

1. Industrial Microbiology; L.M. Prescott, J.P. Harley, D.A. Klein; McGraw Hill Pub.

5th

Edition, (2005).

2. Biotechnology; B.D. Singh; Kalyani Pub., 1st

Edition (2003).

3. Industrial Microbiology; L.E.Casida; New Age International Pub., 1st

Edition, (2004).

4. Principles of Fermentation Technology; P.F. Stanbury, A. Whitaker and S.J. Hall;

Aditya Books Pvt. Ltd., 2nd

Edition, (1997).

5. Biotechnology: Text Book of Industrial Microbiology; Walf Crueger and A.

Crueger; Panima Publishing Corp. 2nd

Edition (2004).


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BBT - 504 PLANT BIOTECHNOLOGY 40hrs

UNIT 1 : Plant Tissue Culture 04

Introduction, History, Concept of cellular totipotency. Morphogenesis, Culture

conditions, medium & its constituents. Method of Sterilization

UNIT 2 : Applications of plant tissue culture- I 06

Callus culture. Cell Suspension culture. Somaclonal variation. Secondary

metabolite production from plant cell cultures.

UNIT 3 : Applications of plant tissue culture-II 10

Micropropagation - Pathways and steps, Introduction to commercial

micropropagation. Embryo culture, embryo rescue, meristem culture. Anther

culture & Ovary culture. Protoplast Culture and Somatic hybridization. Somatic

embryogenesis and Synthetic seeds

UNIT 4 : Methods of genetic engineering in plants 03

Agrobacterium tumefaciens mediated genetic transformation. Direct

methods of gene transfer

UNIT 5 : Genetic manipulation in plants for the following traits 10

Herbicide resistance. Male sterility. Virus resistance, resistance towards

fungal pathogens. Insect resistance. Genetic engineering of plants for

improvement of crop yield and quality. Genetic engineering for

introducing abiotic stress tolerance in plants. Genetic engineering of

nitrogen fixing bacteria; nif genes and it control.


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UNIT 6 : 07

Molecular farming - Production of modified carbohydrate, oil and

recombinant proteins, vaccines in plants. Molecular markers in crop

improvement - Detection of genetic diversity, Marker assisted selection andbreeding.

Recommended Books :

1. Biotechnology: Fundamentals & Applications by S.S.Purohit, Agrobios Ltd.

(2001)2. Trends in Plant Tissue Culture & Biotechnology; L K Pareek and P L

Swarnkar; Published by AgroBios India (2002).

3. Plant Tissue Culture- Theories and Practices; S. S. Bhojwani and M K

Rajdhan; Published by Elsevier, (1996).

4. Methods in Plant Tissue Culture; U Kumar; AgroBios India, (2003)

5. Plant Cell Tissue and Organ Culture Fundamental Methods; O L

Gamborge; Published by Narosa, NewDelhi (2004)

6. Plant Biotechnology: The genetic manipulation of plants; A. Slater, N.

Scott, M. Fowler; Published by

Oxford University press, New York (2003)

7. Transgenic Plants; V. Ranjan, Agrobios


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BBT - 505 GENETIC ENGINEERING 40hrs

UNIT 1: Introduction to Genetic Engineering 02

Scope and history, Handling of DNA, RNA Restriction Enzymes, radioactive

isotopes and other corrosive, carcinogenic and inflammable chemicals/ reagents

UNIT 2: Requirement of genetic engineering 04

Isolation and purification of nucleic acids from living cells. DNA isolation:

Preparation of total cell DNA from animal and plant, Preparation of Plasmid

DNA, preparation of Bacteriophage DNA. RNA isolation: Total Cellular RNA,

Cytoplasmic and NuclearRNA, Poly ( A+) RNA

UNIT 3: Enzymes in recombinant DNA technology 07

Nucleases, Ligases, Poylmerases, DNA modifying enzymes, Topoisomerases,

Restriction endonucleases

UNIT 4: Cloning Vectors: 15

Nomenclature, properties of good vector. Vectors forE. coli: Plasmid and plasmid

based vectors: BAC, Phagemids, cosmids and expression, Virus based vectors:

M-13, Lambda and PAC. Vectors for eukaryotes: Yeast based vectors : 2micro m

plasmid, YEp, YAC, Vectors for plants : Ti plasmid and Ri Plasmid Ca MV,

vectors for Animals - SV-40, Retrovirus vectors and Vaccinia/ Bacculo virus

UNIT 5: In Vitro construction of recombinant DNA molecules 05

Creation of r-DNA, Gene Libraries Genomic DNA and c DNA Cloning

techiniques, Selection of clones based on antibiotic, insertional inactivation,

hybridization, PCR and antibody.

UNIT 6: Methods of DNA transformation in bacteria, plants and animal cells:02

Salt based method, Electroporation, Genegun, Liposome mediated, Transfection


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Recommended Books:

1. Genetic Engineering and its applications. (2004) 2/e, Joshi. P: Agrobios, India

2. Gene Cloning and DNA analysis: An introduction, (2006) 5/e . T. A. Brown,

Black Well Publishing Company.

3. Principles of Gene Manipulation; S. B. Primrose, R. M. Twyman & R. W. old;

Blackwell Science, 6th

Edition (2001).

4. Essential Molecular Biology (volume I) Practical Approach; Edited By T. A.

Brown; Oxford University Press, 2nd

Edition (2001).

5. Molecular Cloning lab manual; Joseph Sambrook, David W. Russell, cold Spring

Harbor Laboratory Press, 3rd

Edition (2001)


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BBT- 506 BIOINFORMATICS (I) 40 hrs

UNIT1: Introductions to Biological Databases 03

Introduction- types, Biological databases, Information Retrieval from Biological

Databases

UNIT 2: Data management of biological information. 03

Concepts, Data management concepts, Data management techniques, Challenges

arising from biological data, Conclusions

UNIT 3: Mining gene expression data. 03

Concepts, Data mining methods for gene expression analysis, Clustering,

Classification, Conclusion and future research

UNIT 4: Protein databases 05

Primary structure databases, Functional protein Databases, Composite sequence

database, Secondary structure database, Structural classification database

UNIT 5: Analyzing DNA, RNA and protein sequences in databases 07Introduction to biological databases, Genbank:organisms in Genbank, genomic

DNA databases, cDNA databases corresponding to expressed genes, Expressed

sequence tags, Sequence tagged sites, Genome survey sequences, High

throughput genomic sequence

UNIT 6: NCBI: introduction, 08

Pubmed, Entrez, Blast, OMIM, Books, Taxonomy, Structure, Locuslink

UNIT 7: Protein database 09

Introduction,annotation,pattern ,family, Swissprot, Prodom, Prints, Pfam,

SMART, MetaFam, iProClass, Popset


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UNIT 8: Molecular modelling 03

Protein databank. accessing PDB entries from NCBI, SCOP: taxononomic system

for protein structures, FSSP database, CATH database, Modelling simulation of

protein structure in silico

Recommended Books:

1. Introduction to Bioinformatics Teresa Atwood and David J.Parry, Pearson smith

publication (2003)

2. Introduction to Bioinformatics lesk, Oxford press (2003)

3. Fundamental Concepts of Bioinformatics - Dan E. Krane, Michael L. Raymer,

Pearson education (2004)

4. Sequence structure and Database Des Higgins, Willice Taylor, oxford press

(2003)

5. Bioinformatics: Sequence and Genome analysis by David W. Mount CBS

Publishers & Distributors, 2004 reprint

6. Bioinformatics: Sequence, Structure and Databanks A Practical Approach,

Higgins, ISBN: 0195667530, I.K. International Publishing House Pvt. Ltd

7. Bioinformatics: Theory and Practice, Chikhale NJ and Gomase VS,

ISBN:9788183188319, Himalaya Publication House

8. Proteomics: Theory and Practice, Gomase VS and Chikhale NJ, Himalaya

Publication House

9. Discovering Genomics, Proteomics and Bioinformatics, Campbell,

ISBN:9788131715598, Pearson Education

10.Bioinformatics: Databases, Tools, and Algorithms, Orpita Bosu, Simminder Kaur,

Thukral , ISBN: 9780195676839, Oxford University Press


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Practical I

1. Estimation of vitamin C

2. Estimation of RNA by orcinol method

3. Estimation of DNA by DPA method

4. Estimation of protein by Folin- Lowry method

5. Titration curve of amino acid - glycine

6. Isolation of plasmid DNA

7. Separation of plasmid DNA by agarose gel electrophores

8. Bacterial transformation

9. Bacterial conjugation

10. Restriction digestion of DNA


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Practical II

1. Introduction to plant tissue culture laboratory and equipments2. Preparation of plant tissue culture media3. Establish the cultures from suitable explants to demonstrate various pathways of

micropropagation:

Axillary shoot bud proliferation

In vitro adventitious shoot bud differentiation

Callus induction and callus organogenesis

4. Inoculate the tissue culture raised shoots on suitable medium for in vitro and exvitro rooting

5. Hardening and acclimatization ofin vitro raised rooted shoots6. Encapsulate the shoot buds/ seeds to demonstrate the production of synthetic

seeds

7. Perform anther culture to demonstrate the production of haploid plants8. Perform meristem culture to demonstrate the production of virus-free plants9. Effect of plant growth regulators on callus induction : effect of harmone variation

: effect of charcoals

10.Embryo Culture


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BBT - 601 NEUROBIOLOGY AND BEHAVIOURAL SCIENCE 40 hrs

UNIT 1 : Overview of the Nervous system and anatomy of the Nervous system 02

UNIT 2: Introduction to Neurobiology 04

The neuron. Electrophysiology of the Neuron. Electrical signals. Membrane

permeability. Channels and pumps. Synaptic transmission. Neurotransmitter-

neurotransmitter receptors and their effects. Synaptic plasticity.

UNIT 3 : Modulation of movement 02

Construction of Neural Circuits. Modification of Brain Circuits.

UNIT 4 : Electrical Signaling and Neurons communication 06

Electrical Signaling within the Neuron. The chemical senses. Spinal cord

circuits and motor control. Descending control of spinal cord circuitry.

Introduction to Synaptic transmission. Synaptic integration & Modulation.

Neurotransmitters and their release.

UNIT 5 : Neurophysiology and Cognition 02

Sensory Systems, Motor Systems

UNIT 6 : Study of Behaviour 02

Variousapproaches, innate behaviour, learned behaviour

UNIT 7 : Core Competency and Building Positive Attitude 05

Self confidence, Self image. Meaning and nature of attitude. Components and

Types of attitude. Importance and relevance of attitude

UNIT 8 :Thinking as a tool for Problem Solving and Plan of Action 05

What is thinking: The Mind/Brain/Behavior. Critical Thinking and Learning.

Making Predictions and Reasoning. Construction of POA. Monitoring.

Reviewing and analyzing the outcome.


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UNIT 9 : Creative Thinking 03

Definition and meaning of creativity. The nature of creative thinking -

Convergent and Divergent thinking, Idea generation and evaluation (Brain

Storming).

UNIT 10 : Interpersonal Relationship Development 03

Importance of Interpersonal Relationships. Interpersonal Relationships Skills.

Impression Management:Meaning & Components of Impression Management.

Impression Management Techniques(Influencing Skills)

UNIT 11 : Teams 03

Meaning and nature of teams. External and internal factors effecting team.

Leadership- styles in organization, Leadership in Teams

UNIT 12 : Managing Personal Effectiveness 03

Setting goals to maintain focus. Dimensions of personal effectiveness ( self

disclosure, openness to feedback and perceptiveness). Time management.

Identifying long-term goals.

Recommended Books:

1. Neurobiology:Molecules,Cell and Systems.(second-edition),

GaryG.Matthews,Blackwell Science.

2. Vangelist L. Anita, Mark N. Knapp, Inter Personal Communication and Human

Relationships: Third Edition.

3. Bates, A. P. and Julian, J.: Sociology - Understanding Social Behaviour.

4. Phil Lowe Koge Page: Creativity and Problem Solving.


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5. Dick, Mc Cann & Margerison, Charles: Team Management, 1992 Edition.

6. Geoff Petty: How to be better at creativity?

7. Organizational Behaviour, Davis, K.


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BBT - 602 ENVIRONMENTAL BIOTECHNOLOGY 40hrs

UNIT 1 : Environmental Biotechnology: Vision and Development 02

Biodiversity, ecology. Pollution control. Agriculture. Bioenergy

UNIT 2 : Biodiversity and Ecology 05

Biosphere. Ecosystem. Natural cycles (Nitrogen, Carbon, Phosphorus, Sulfur).

Energy flow.

UNIT 3 : Environmental Pollution and Control 16

Water pollution. Sources of pollutants. Waste water processing. Advanced

waste water treatment in different industries (Brewery, Petroleum, paper and

pulp). Soil pollution. Sources of pollutants. Bioremediation - In-situ (intrinsic,

genetically engineered), Ex-situ (solid phase treatment slurry phase treatment,

vermicomposting). Air pollution - Sources of pollutants, Removal and control.

Hazardous waste management - Physical treatment, Chemical treatment,

Biological treatment. Xenobiotic compounds and their degradation -

Characteristics of xenobiotic compounds, Examples of xenobiotic compounds,

Mechanism of degradation

UNIT 4 : Environmental Impact Assessment 03

Need and objectives of EIA. Factors involved in EIA. Environmental attributes.

Methods of EIA - Ad-hoc, Overlay, Network, Matrix, Checklist, Cost benefit,

limitations and problems of EIA

UNIT 5 : Global Warming and Green House Effect 02

Reasons for global warming. Technique of Green house effect. Applications of

Green house effect


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UNIT 6 : Agricultural Biotechnology 08

Pesticides - Insecticides- types, mode of action and applications, Herbicides-

types, mode of action and applications, Fungicides- types, mode of action and

applications. Biopesticides -B. thuringensis- mode of action and applications,

Viral pesticides- example, mode of action and applications, Neem pesticides-

mode of action and applications. Biofertilizers - Nitrogen fixing organisms-

example, mode of action and applications, Phosphorus solublising organisms-

example, mode of action and applications. Genetically modified organisms

Need, Application, Stress tolerant plants

UNIT 7 : Bioenergy 03

Renewable sources of energy. Biomass as a source of energy. Energy crops

Production, Applications

UNIT 8 : Integrated Environmental Management (overview) 01

Recommended Books:

1. Environmental Biotechnology; M. H. Fulekar; Oxford & IBH Publishing Company pvt

ltd; 1st

edition

2. Environmental Biotechnology; Dilipkumar Markandey; APH Publishing Corporation;

1st

Edition

3. Environmental Biotechnology: theory and applications; G. Evans & J. Furlong; Wiley

& Sons pvt ltd; 1st

edition

4. Textbook of Environmental Engineering; P. Venugopal Rao; PHI pvt ltd; 1st

edition

5. Environmental Biotechnology; Alan Sragg; Oxford University Press; 2nd edition

6. Biotechnology; B. D. Singh; Kalyani Publications; 1st

edition

7. Water and Waste Water Technology; Hammer & Hammer Jr.; PHI pvt ltd; 4th

edition

8. Green House Technology for controlled Environment; G N Tiwary; Narosa Publishing

House; 1stedition


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BBT - 603 BIOPROCESS TECHNOLOGY 40hrs

UNIT 1: Introduction to Fermentation process. 02

Upstream Process, Downstream Process, Range of fermentation process,

Component parts of fermentation process

UNIT 2: Media for Industrial fermentations 07

Medium Sterilization. Batch Sterilization: Continuous sterilization, Fermentor

Sterilization, Feed Sterilization, Filter sterilization

UNIT 3: Bioreactor Design 04

Construction material, Aeration and agitation, Sampling/ Feed port, Valves and

steam traps

UNIT 4: Growth kinetics and stoichiometry 06

Batch Culture, Continuous culture, Fed Batch Culture

Stoichiometric calcn: Elemental Balance,Yield coefficient.

UNIT 5: Types of Reactor 04Stirred tank reactor, Acetators and Cavitators, Air Lift Reactor, Bubble Column

Reactor, Packed Bed Reactor, Fluidized Bed Reactor, Photobioreactor

UNIT 6: Solid state Fermentation 06

Bioreactors in solid state fermentation, Submerged Fermentation

UNIT 7: Process monitoring and control. 02

Physical and chemical sensors for medium and gases, Online/ Offline sensors

UNIT 8: Microbial interactions 06

Effluent treatment, Process Economics, Scale up procedure from lab scale to pilot

scale


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Recommended Books:

1. Biochemical Engineering (1997) Blanch HW and Clark DS, Marcel Dekker Inc.,

USA.

2. Biochemical Engineering and Biotechnology (2007) Najafpour GD, Elsevier,

Netherlands.

3. Biochemical Engineering Fundamentals (1986) (2/e) Bailey JE and Ollis DF,

McGraw-Hill International Editions CES, Singapore.

4. Bioprocess Engineering Principles (1995) Doran PM, Academic Press Ltd, USA.

5. Bioprocess Engineering: Basic Concepts (2002) Shuler ML and Kargi F, Pearson

Education Pvt. Ltd., Singapore.

6. Biotechnology (1993) (2/e) Vols. 3 and 4 Rehm HJ and Reed G, VCH, Germany.

7. Comprehensive Biotechnology Vol. 2 (1985) Moo-Young M, Pergamon Press Ltd.,

UK.

8. Fermentation Microbiology and Biotechnology (1999) El-Mansi EMT and Bryce

CFA, Taylor and Francis, USA.

9. Manual of Industrial Microbiology and Biotechnology (1999) (2/e) Demain AL and

Davies, ASM, USA.

10.Principles of Fermentation Technology (1995) (2/e) Stanbury PF, Whitaker A and

Hall SJ, Butterworth-Hienemann Ltd., UK.


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UNIT 7 : Applications of animal biotechnology 06

Use of cell culture for production of a regulatory protein. Use of cell culture for

production of a hormone (e.g. Insulin). Use of cell culture for production of

vaccines. Cell hybridization and human hybridization. Use of cell culture in

drug targeting and drug toxicity analysis. Transplantation of cultured cells

UNIT 8 : Stem cell Biotechnology 03

Types and characteristics of stem cells. Culture conditions/equipments essential

for embryonic stem cell cultures. Use of stem cell cultures for treatment of

genetic disorders

UNIT 9 : Transgenic animals 03

Methods of transgenesis. Vectors involved. Biopharmaceutical applications of

transgenic animals.

Recommended books :

1. C. Helgasson; Basic cell culture protocols, 3rd edition, Human press

2. E. D. Rang, H.P. Dale, M.M. Ritter; Pharmacology, 5th edition

3. J. Mather and d. Barnes; Animal cell culture methods, Elsevier, vol 57

4. J.R.W. Masters; Animal Cell Culture- A practical approach, Oxford university

press

5. J. Paul Basic Protocols in cell and tissue culture

6. M. Butler; Animal cell technology-Principles and products, Open University press

7. M. Butler and M. Dawson, Cell culture lab fax, Bios scientific Pvt. Ltd.

8. M. Cylnes; Animal cell culture techniques, Springer Verlag

9. M.M. Young; Animal Biotechnology, Pergamon press, Oxford

10.N. Jenkins; Animal cell biotechnology-Methods and protocols, Human Press

11.R.I. Freshney; Culture of animal cells: A manual of basic techniques, John Wiley

and sons, 4th

edition.


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BBT - 605 GENETIC ENGINEERING- II 40hrs

UNIT 1: Production of therapeutic agents: 12

Engineeri

d.y.patil department of biotechnology syllabus

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