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Dr. Alvin Yeh Department of Biomedical Engineering Blood Harvest: Laboratory Creation of Blood Vessels Josh Bergerson Normangee High School

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Virtual Model of Angiogenesis Important Process In: normal tissue repairmultiple diseasesengineered tissue.

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In Vitro Angiogenesis Model Can study the effects of Varying collagen stiffness Varying collagen thickness Varying collagen concentration Endothelial cell monolayer Collagen gel preparation Serumfree medium

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The Results

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Electromagnetic spectrum Properties of waves Experimental design Optics & imaging PolymersFluorescenceMechanics PC data collection Elements of Research Applied in the Classroom

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Relevance to Standards

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Learning Objectives

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Materials Borax Cardboard Silicon mold Computer paper Corn meal Sugar Salt Baking soda Clear glue Water Gelatin Fishing line Water Laser pointers(r/g/b) Meter stick Weight set Spring scale Ruler Beakers Craft sticks Binder clips Calculators Force sensor (opt) Light sensor (opt) Go-link(opt) scissors packing tape Food coloring (opt)

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Classroom Lesson Day 1

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Classroom Lesson Day 2 Matrix Constraints Must be smaller than 5cm x 5cm x 2cm Must contain fishing line Must be transparent Matrix Preparation Make mold (silicon/Cardboard) Mix polymer matrix Add additives (opt) Let gels set overnight Corn meal Salt Sugar Baking Powder

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Classroom Lesson Day 3 Mechanical Testing Force sensor to measure x/y strength Mass set to test z strength Imaging the Sample Image with R/G/B Record diameter of sample Light sensor to gauge transparency of gel Testing the Matrix

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Classroom Lesson Day 4 Image Analysis Use diffraction pattern to find diameter of sample Compare results of intensity with R/G/B Concluding Presentation Present matrix & data to class Suggest improvements they would make Compare class data to find most efficient model

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Sample Pre- and Post- Test Questions Which of the following is the result of excessive angiogenesis? A stroke Cancer Tissue growth Tissue Repair Which color of visible light has the most energy? Red Green Blue Ultraviolet (True / False) Polymers with different chemical compositions may have different physical properties. (True / False) A polymers color is an example of an extensive property.

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Sources From the Web uwbwritingcenter.wordpress.com en.wikipedia.org stacymakescents.com unschoolme.blogspot.com http://stemak.org/sites/default/files/Measuring%20the%2 0diameter%20of%20a%20hair%20using%20a%20laser.pdf http://stemak.org/sites/default/files/Measuring%20the%2 0diameter%20of%20a%20hair%20using%20a%20laser.pdf http://www.vernier.com/products/sensors/force- sensors/dfs-bta/ biology.clc.uc.edu

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Acknowledgements TAMU E3 RET Program National Science Foundation Nuclear Power Institute Dr. Alvin Yeh Research Group: Tissue Microscopy Lab Dr. Arne Lekven Lab Group