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Final stuff:Final stuff: Lab practical: Apr 29Lab practical: Apr 29
Final exam: due Fri May 7 @ Final exam: due Fri May 7 @ 2:152:15
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TodayToday
IDEM samplingIDEM sampling
Assessment exerciseAssessment exercise
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MACROINVERTEBRATE COMMUNITY
MONITORING EXAMPLE
INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
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STAGES OF BIOLOGICAL COMMUNITY ASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING LABORATORY PROCESSING
SAMPLE COLLECTION
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EcologicalIntegrity
What are We Trying to Measure ??
Water Quality
Habitat, Flow regime
Biological Interactions
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“The ability of an aquatic ecosystem to support and maintain a balanced, integrated, adaptive community of
organisms having a species composition, diversity, and functional organization
comparable to the best natural habitats within a region.”
BIOLOGICAL INTEGRITY
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STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
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Aquatic Larvae
Aquatic Adults
Terrestrial Adults
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Adult and Larval Caddisflies
(Trichoptera)
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USEPA Rapid Bioassessment Protocols
Riffle Habitats
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Two Composite
KICK Screen Samples
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STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
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Sample Elutriation
Remove Gravel and Sand
Remove Excess CPOM
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The Final Sample Ready for
Preservation
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Field Preservation of Samples
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GPS for Spatial Reference
PAPERWORK…PAPERWORK…. TRIPLE REDUNDANCY
Water Chemistry
Habitat Assessments
Field Observations
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STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
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All Samples are Returned to Laboratory for Processing
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Squares (25 cmSquares (25 cm22))
Removed by Suction to Removed by Suction to Petri DishPetri Dish
100 Organism Sub-Sample
15 Minute Sort Sub-Sample
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100 Organism Sub-Sample
Selected at Random
Second Sub-Sample 15 Minutes
Entire Pan “Randomly” Sorted
Using Forceps
Final Sort Under Microscope
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Sub-Samples Processed to Sub-Samples Processed to Family LevelFamily Level
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All Samples Preserved for Permanent Museum Storage
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STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
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TEN SUB-METRICS
SITE INFORMATIO
NTAXONOMIC
INFORMATION
AND
TAXA COUNTS
LABORATORY BENCHSHEET
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Example of 2 Sub-Metrics
E
P
T
EPT Index = 6 Families
EPT Count = 7 Individuals
Metric Scores = 0, 2, 4, 6, 8
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SCORING CRITERIA FOR THE FAMILY LEVELMACROINVERTEBRATE INDEX OF BIOTIC INTEGRITY
(mIBI)USING PENTASECTION AND CENTRAL TENDENCY ON THE
LOGARITHMIC TRANSFORMED DATA DISTRIBUTIONS OF THE 1990-1994 RIFFLE KICK SAMPLES
CLASSIFICATION SCORE
8 6 4 2 0
Family LevelHBI
4.08 4.09 - 4.54 4.55- 5.05 5.06 - 5.62 5.63
Number ofTaxa
18 17-15 14-11 10-8 7
Number ofIndividuals
350 349-213 212-130 129-80 79
PercentDominant
Taxa 22.1 22.2-31.1 31.2-43.8 43.9-61.5 61.6
EPTIndex
8 7-6 5-4 3 2
EPTCount
195 194-92 91-43 42-20 19
EPTCount To
TotalNumber ofIndividuals
0.69 0.68-0.47 0.46-0.30 0.29-0.14 0.13
EPT CountTo
ChironomidCount
11.66 11.65-5.71 5.70-2.56 2.55-0.89 0.88
ChironomidCount
6 7-19 20-54 55-146 147
TotalNumber ofIndividualsTo Numberof Squares
Sorted
410 409-172 171-72 71-30 29
Scoring Criteria
for Riffle KICK
Samples
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Not All Rivers and Streams Have Riffle Habitats
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Multi-Plate Samples
are Collected
IndividuallyMulti-Plate
Samples are Preserved in the Field and
Scraped in the Laboratory
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Systematic Community Assessments of Benthic
Macroinvertebrate Communities are Effective
SUMMARY
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IBI -- Family Level Multi-Metric Indices Provide:
Cost Effective Data of Ecological Health of Rivers and Streams
Assessment Information to Make
Management Decisions
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Lammert & Allen 1999Lammert & Allen 1999Scoring Classification
Metric 1 3 5
Total Taxa < 22 23 - 30 > 30
Mayfly Taxa 0 - 3 4 - 6 > 6
Caddisfly Taxa 2 - 3 4 - 5 > 5
Dipteran Taxa 3 - 4 5 - 6 > 6
Relative Abundance of
Omnivores
70 – 100 % 57 – 69 % 43 – 56 %
Relative Abundance of
Filterers
51 – 69 % 50 – 31 % 9 – 30 %
Relative Abundance of
Grazers
0 – 19 % 20 – 32 % > 33 %
Relative Abundance of
Predators
24 – 33 % 14 – 23 % 2 – 13 %
Dominance 68 – 88 % 47 – 67 % 24 – 46 %
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Actual Observation
IBI Score
(no.) (no.)
Total Taxa 19 1Mayfly Taxa 2 1Caddisfly Taxa 2 1Dipteran Taxa 3 1Relative Abundance of Omnivores 0.42 5Relative Abundance of Filterers 0.02 5Relative Abundance of Grazers 0.01 1Relative Abundance of Predators 0.43 1Dominance 0.30 5
B-IBI score Lammert & Allan (1999) Total IBI 21