final stuff: n lab practical –coleoptera, hemiptera n final exam: fri may 2 @ 2:15 –assessment...
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Final stuff:Final stuff: Lab practicalLab practical
– Coleoptera, HemipteraColeoptera, Hemiptera
Final exam: Fri May 2 @ 2:15Final exam: Fri May 2 @ 2:15– Assessment with InvertebratesAssessment with Invertebrates
Lecture material (IDEM protocol)Lecture material (IDEM protocol) Stone et al. (2005): Ag streams in Stone et al. (2005): Ag streams in
IllinoisIllinois Signatures of ICI; Ohio EPA use of ICI Signatures of ICI; Ohio EPA use of ICI
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TodayToday
IDEM samplingIDEM sampling
Stone et al. (2005) paperStone et al. (2005) paper
Assessment exerciseAssessment exercise
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MACROINVERTEBRATE COMMUNITY
MONITORING EXAMPLE
INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
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STAGES OF BIOLOGICAL COMMUNITY ASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING LABORATORY PROCESSING
SAMPLE COLLECTION
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EcologicalIntegrity
What are We Trying to Measure ??
Water Quality
Habitat, Flow regime
Biological Interactions
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“The ability of an aquatic ecosystem to support and maintain a balanced, integrated, adaptive community of
organisms having a species composition, diversity, and functional organization
comparable to the best natural habitats within a region.”
BIOLOGICAL INTEGRITY
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STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
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Aquatic Larvae
Aquatic Adults
Terrestrial Adults
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Adult and Larval Caddisflies
(Trichoptera)
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USEPA Rapid Bioassessment Protocols
Riffle Habitats
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Two Composite
KICK Screen Samples
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STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
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Sample Elutriation
Remove Gravel and Sand
Remove Excess CPOM
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The Final Sample Ready for
Preservation
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Field Preservation of Samples
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GPS for Spatial Reference
PAPERWORK…PAPERWORK…. TRIPLE REDUNDANCY
Water Chemistry
Habitat Assessments
Field Observations
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STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
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All Samples are Returned to Laboratory for Processing
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Squares (25 cmSquares (25 cm22))
Removed by Suction to Removed by Suction to Petri DishPetri Dish
100 Organism Sub-Sample
15 Minute Sort Sub-Sample
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100 Organism Sub-Sample
Selected at Random
Second Sub-Sample 15 Minutes
Entire Pan “Randomly” Sorted
Using Forceps
Final Sort Under Microscope
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Sub-Samples Processed to Sub-Samples Processed to Family LevelFamily Level
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All Samples Preserved for Permanent Museum Storage
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STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
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TEN SUB-METRICS
SITE INFORMATIO
NTAXONOMIC
INFORMATION
AND
TAXA COUNTS
LABORATORY BENCHSHEET
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Example of 2 Sub-Metrics
E
P
T
EPT Index = 6 Families
EPT Count = 7 Individuals
Metric Scores = 0, 2, 4, 6, 8
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SCORING CRITERIA FOR THE FAMILY LEVELMACROINVERTEBRATE INDEX OF BIOTIC INTEGRITY
(mIBI)USING PENTASECTION AND CENTRAL TENDENCY ON THE
LOGARITHMIC TRANSFORMED DATA DISTRIBUTIONS OF THE 1990-1994 RIFFLE KICK SAMPLES
CLASSIFICATION SCORE
8 6 4 2 0
Family LevelHBI
4.08 4.09 - 4.54 4.55- 5.05 5.06 - 5.62 5.63
Number ofTaxa
18 17-15 14-11 10-8 7
Number ofIndividuals
350 349-213 212-130 129-80 79
PercentDominant
Taxa 22.1 22.2-31.1 31.2-43.8 43.9-61.5 61.6
EPTIndex
8 7-6 5-4 3 2
EPTCount
195 194-92 91-43 42-20 19
EPTCount To
TotalNumber ofIndividuals
0.69 0.68-0.47 0.46-0.30 0.29-0.14 0.13
EPT CountTo
ChironomidCount
11.66 11.65-5.71 5.70-2.56 2.55-0.89 0.88
ChironomidCount
6 7-19 20-54 55-146 147
TotalNumber ofIndividualsTo Numberof Squares
Sorted
410 409-172 171-72 71-30 29
Scoring Criteria
for Riffle KICK
Samples
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Not All Rivers and Streams Have Riffle Habitats
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Multi-Plate Samples
are Collected
IndividuallyMulti-Plate
Samples are Preserved in the Field and
Scraped in the Laboratory
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Systematic Community Assessments of Benthic
Macroinvertebrate Communities are Effective
SUMMARY
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IBI -- Family Level Multi-Metric Indices Provide:
Cost Effective Data of Ecological Health of Rivers and Streams
Assessment Information to Make
Management Decisions