Dr. Myrto-Panagiota Zacharof
Supervisor: Dr.R.W.Lovitt
The Production Of Bacteriocins From
Lactic Acid Bacteria
3L.plantarum
L.casei
L.lactis
• Media Optimisation
• Extraction strategy
• Chemical nature
Protein assay
trypsination
Sizing
• Quantification Assay
y = -0.0019x + 0.3453R2 = 0.9758
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0 50 100 150 200Nisin IU/ml
μ m
ax (h
-1)
μ max (h-1) of L.delbruckii under a
range of Nisin Concentrations
between 0-200 IU/mLinear fitting is used
• Concentration &Purification Method
• Self Life
ResultsThe 3 strains are indeed producing bacteriocins
• Chemical Nature
Results
0
10
20
30
40
50
1 10 100 1000 10000
Volume (%
)
Size (d.nm)
Statistics Graph (8 measurements)
Mean w ith Max-Min error bar
• Bacteriocins Activity
Results
Nisin &Bacteriocins
Target strains
• Bacteriocins Potency
Results
Bacteriocins
Target Strain
• Self LifeResults
The Finishing Line
• Further Developments
The Finishing Line
Acknowledgments
Dr.R.W.Lovitt Dr.M.Jones Dr.P.Williams Dr.I.Jung
