FoxN1-/-

Day

1D

ay3

Day

7

38.8% 0.5%

12.2% 48.5%

3.6% 0.2%

15.8% 80.4%B220

CD

3

Wild Type FoxN1-/-A

B

0

10

20

30

40

50

60

T cell B cell

Wild Type

FoxN1-/-

C

Tota

l cel

l # /s

plee

n(×1

0 )

Figure S2. FoxN1-/- mice displayed muscle regeneration defects, Related to Figure 1(A) FACS analysis of T (CD3+) and B (B220+ ) cell profiles in spleen from wild type or FoxN1-/- mice. (B) Statistical analysis of T (CD33 independent experiments.

+) and B (B220+ ) cell numbers in spleen from wild type or

FoxN1-/- mice. Error bars were based on

(C) FACS analysis of CD4+ and CD8+ T cells infiltrated in TA muscles at day 3 post muscle injury in wild type or FoxN1-/- mice.(D) FACS analysis of T (CD3+ ) and B (B220 + ) cell profiles in TA muscles day 3 post muscle injury in wild type or FoxN1-/- mice.(E) HE staining of muscle tissue sections from wild type or FoxN1-/- mice 1, 3, and 7 days after muscle injury. Scale bars, 200 μm.(F) Statistical analysis of the number of myofibers with centrally located nuclei at day 7 post muscle injury in wild type or FoxN1(G) Statistical analysis of the myofiber size 12 days post injury in wild type or FoxN1myofiber sizes was based on average of 3 independent experiments.

-/- mice. ** indicated statistically significant, p < 0.01. Error bars were based on 3 independent experiments.

Wild Type

CD

8

CD4

D

E

9.4% 0.3%

58.7% 31.6%

0.3% 0.1%

0.2%99.7%

Wild Type FoxN1-/-

FoxN1

Wild Typ

e

**

100

150

200

250

# of

myo

fiber

s w

ith c

entr

ally

loca

ted

nucl

ei/fi

eld

F

-/-

0

10

20

30

40

50

60 FoxN1-/-Wild Type

200 400 600 800 1000 12000

% o

f myo

fiber

are

a

Myofiber area(μm2)

G

6

-/- mice. The distribution map of the different

B220

CD

3

41.6% 0.6%

53.0% 4.8%

2.7% 0.4%

62.4% 34.9%

Wild Type FoxN1-/-

Fu_Figure-S2