38.8% 0.5% 3.6% 0.2% day1 cd3 b220 - nature research · day1 day3 day7 38.8% 0.5% 12.2% 48.5% 3.6%...
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FoxN1-/-
Day
1D
ay3
Day
7
38.8% 0.5%
12.2% 48.5%
3.6% 0.2%
15.8% 80.4%B220
CD
3
Wild Type FoxN1-/-A
B
0
10
20
30
40
50
60
T cell B cell
Wild Type
FoxN1-/-
C
Tota
l cel
l # /s
plee
n(×1
0 )
Figure S2. FoxN1-/- mice displayed muscle regeneration defects, Related to Figure 1(A) FACS analysis of T (CD3+) and B (B220+ ) cell profiles in spleen from wild type or FoxN1-/- mice. (B) Statistical analysis of T (CD33 independent experiments.
+) and B (B220+ ) cell numbers in spleen from wild type or
FoxN1-/- mice. Error bars were based on
(C) FACS analysis of CD4+ and CD8+ T cells infiltrated in TA muscles at day 3 post muscle injury in wild type or FoxN1-/- mice.(D) FACS analysis of T (CD3+ ) and B (B220 + ) cell profiles in TA muscles day 3 post muscle injury in wild type or FoxN1-/- mice.(E) HE staining of muscle tissue sections from wild type or FoxN1-/- mice 1, 3, and 7 days after muscle injury. Scale bars, 200 μm.(F) Statistical analysis of the number of myofibers with centrally located nuclei at day 7 post muscle injury in wild type or FoxN1(G) Statistical analysis of the myofiber size 12 days post injury in wild type or FoxN1myofiber sizes was based on average of 3 independent experiments.
-/- mice. ** indicated statistically significant, p < 0.01. Error bars were based on 3 independent experiments.
Wild Type
CD
8
CD4
D
E
9.4% 0.3%
58.7% 31.6%
0.3% 0.1%
0.2%99.7%
Wild Type FoxN1-/-
FoxN1
Wild Typ
e
**
100
150
200
250
# of
myo
fiber
s w
ith c
entr
ally
loca
ted
nucl
ei/fi
eld
F
-/-
0
10
20
30
40
50
60 FoxN1-/-Wild Type
200 400 600 800 1000 12000
% o
f myo
fiber
are
a
Myofiber area(μm2)
G
6
-/- mice. The distribution map of the different
B220
CD
3
41.6% 0.6%
53.0% 4.8%
2.7% 0.4%
62.4% 34.9%
Wild Type FoxN1-/-
Fu_Figure-S2