Download - 2012 Chapter 08 - Transcription
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010
TRANSCRIPTION
CHAPTER - 08
8.1: Overview of Transcription8.2: RNA Polymerase Core Enzyme8.3: Promoter Recognition in Bacteria and Eukaryotes8.4: Initiation of Transcription and Transition to an Elongating Complex8.5: Transcription Elongation8.6: Transcription Termination8.7: Principles of Transcription Regulation8.8: DNA-Binding Domains in Transcriptional Regulators8.9: Mechanisms for Regulating Transcription in Bacteria8.10: Competition Between cl and Cro and the Fate of Bacteriophage Lambda8.11: Mechanisms for Modulating Eukaryotic Transcription8.12: Combinatorial Regulation of Eukaryotic Transcription8.13: Signaling Cascades and Regulation of Transcription8.14: Regulation of Elongation and Termination by RNA and Proteins8.15: Transcriptional Silencing
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-018.1: Overview of Transcription
8.1 Overview of Transcription
The Central Dogma
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-02
The Stages
Initiation
Elongation
Termination
8.1: Overview of Transcription
8.1 Overview of Transcription
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-038.2: RNA Polymerase Core Enzyme
RNA polymerases are made up of different subunits
Bacteria and archaebacteria
Eukaryotes
8.2 RNA Polymerase Core Enzyme
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-05
8.3 Promoter Recognition in Bacteria
The promoter
The polymerase
The sigma factor
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010
Figure 08-06
8.3 Promoter Recognition in Bacteria
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010
Figure 08-10
8.3 Promoter Recognition in Eukaryotes
General Transcription Factors
TBP
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010
Figure 08-12
8.3 Promoter Recognition in Eukaryotes
The Pre-initiation Complex RNA Pol II
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-138.3: Promoter Recognition in Bacteria and Eukaryotes
8.3 Promoter Recognition in Eukaryotes
RNA Pol I
RNA Pol III
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-148.4: Initiation of Transcription and
Transition to an Elongating Complex
8.4 Initiation of Transcription
The Closed Complex
The Open Complex
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-158.4: Initiation of Transcription and
Transition to an Elongating Complex
8.4 Initiation of Transcription
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-188.4: Initiation of Transcription and
Transition to an Elongating Complex
8.4 Initiation of Transcription
Transition to the elongation complex
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-198.5: Transcription Elongation
After transition to an elongation complex, transcription is highly processive
8.5 Transcription Elongation
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-218.5: Transcription Elongation
8.5 Transcription Elongation
Transcriptional pausing
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-228.5: Transcription Elongation
Transcriptional pausing
8.5 Transcription Elongation
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-238.5: Transcription Elongation
8.5 Transcription Elongation
Linking transcription with RNA processing
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 8.5: Transcription Elongation
8.5 Transcription Elongation
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-26a8.6: Transcription Termination
Bacterial intrinsic terminators have two main features:
In E. coli, certain genes need the Rho protein to terminate transcription (these are Rho-dependent terminators;
8.6 Bacterial Transcription Termination
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-28a8.6: Transcription Termination
Termination of RNA Pol II genes is coupled to processing of the 3′ end of the mRNA
Allosteric:
8.6 Eukaryote Transcription Termination
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Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-28b8.6: Transcription Termination
It was found that termination is linked to degradation of the cleaved RNA fragment.
Torpedo model of termination:
8.6 Eukaryote Transcription Termination