2012 chapter 08 - transcription

Craig et al: Molecular Biology Copyright © Oxford University Press 2010

TRANSCRIPTION

CHAPTER - 08

8.1: Overview of Transcription8.2: RNA Polymerase Core Enzyme8.3: Promoter Recognition in Bacteria and Eukaryotes8.4: Initiation of Transcription and Transition to an Elongating Complex8.5: Transcription Elongation8.6: Transcription Termination8.7: Principles of Transcription Regulation8.8: DNA-Binding Domains in Transcriptional Regulators8.9: Mechanisms for Regulating Transcription in Bacteria8.10: Competition Between cl and Cro and the Fate of Bacteriophage Lambda8.11: Mechanisms for Modulating Eukaryotic Transcription8.12: Combinatorial Regulation of Eukaryotic Transcription8.13: Signaling Cascades and Regulation of Transcription8.14: Regulation of Elongation and Termination by RNA and Proteins8.15: Transcriptional Silencing

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-018.1: Overview of Transcription

8.1 Overview of Transcription

The Central Dogma

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-02

The Stages

Initiation

Elongation

Termination

8.1: Overview of Transcription

8.1 Overview of Transcription

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-038.2: RNA Polymerase Core Enzyme

RNA polymerases are made up of different subunits

Bacteria and archaebacteria

Eukaryotes

8.2 RNA Polymerase Core Enzyme

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-05

8.3 Promoter Recognition in Bacteria

The promoter

The polymerase

The sigma factor


Figure 08-06

8.3 Promoter Recognition in Bacteria


Figure 08-10

8.3 Promoter Recognition in Eukaryotes

General Transcription Factors

TBP


Figure 08-12


The Pre-initiation Complex RNA Pol II

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-138.3: Promoter Recognition in Bacteria and Eukaryotes


RNA Pol I

RNA Pol III

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-148.4: Initiation of Transcription and

Transition to an Elongating Complex

8.4 Initiation of Transcription

The Closed Complex

The Open Complex




Transition to the elongation complex

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-198.5: Transcription Elongation

After transition to an elongation complex, transcription is highly processive

8.5 Transcription Elongation



Transcriptional pausing



Linking transcription with RNA processing

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-26a8.6: Transcription Termination

Bacterial intrinsic terminators have two main features:

In E. coli, certain genes need the Rho protein to terminate transcription (these are Rho-dependent terminators;

8.6 Bacterial Transcription Termination

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-28a8.6: Transcription Termination

Termination of RNA Pol II genes is coupled to processing of the 3′ end of the mRNA

Allosteric:

8.6 Eukaryote Transcription Termination

Craig et al: Molecular Biology Copyright © Oxford University Press 2010 Figure 08-28b8.6: Transcription Termination

It was found that termination is linked to degradation of the cleaved RNA fragment.

Torpedo model of termination:

8.6 Eukaryote Transcription Termination

2012 chapter 08 - transcription

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