Download - 11-20-15 Presentation
Transcript
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Manipulating Cell Migration Using
Amphiphiles11/20/15 Meeting
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Last Time
• Tried new PDMS method
• Preliminary results were encouraging
• Contamination ended the experiment
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Universal Cell Migration Assembly
Created our own version of the assembly using PDMS and a 24 well plate
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First AttemptGoal was to create a column that would adhere to
the bottom of the well when pressure was applied by lid of well plate
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Next Try
PDMS columns much stronger although diameter is larger
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Some Images: BA
• Boundary very well-defined
• Images easily reproducible
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Control
• It appears more cells have migrated
• DAPI again ineffective
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Next MeetingImages will be stitched together with better
softwareCells will be counted (hopefully more
accurately)