Manipulating Cell Migration Using

Amphiphiles11/20/15 Meeting

Last Time

• Tried new PDMS method

• Preliminary results were encouraging

• Contamination ended the experiment

Universal Cell Migration Assembly

Created our own version of the assembly using PDMS and a 24 well plate

First AttemptGoal was to create a column that would adhere to

the bottom of the well when pressure was applied by lid of well plate

Next Try

PDMS columns much stronger although diameter is larger

Some Images: BA

• Boundary very well-defined

• Images easily reproducible

Control

• It appears more cells have migrated

• DAPI again ineffective

Next MeetingImages will be stitched together with better

softwareCells will be counted (hopefully more

accurately)