dormancy of cells and organisms - strategies for survival and preservation nest - # 012674 2005-2008...
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Dormancy of cells and organisms -strategies for survival and
preservation
NEST - # 0126742005-2008
Acronym: Sleeping Beauty
Partners
• Esther Lubzens (NIO) Ora Hadas, Assaf Sukenik (KLL) -IOLR –P1
• Joan Cerdà, IRTA, Barcelona, Spain – P2
• Stefan Hohmann, Goteborg University, Sweden – P3
• Melody Clark and Roger Worland, BAS, Cambridge, UK – P4
• Richard Reinhardt, Max Planck Institute –Molecular Genetics, Berlin-Dahlem, Germany – P5
• Jens Nielson, Danish Technical University, Lyngby, Denmark - P6
Objectives
Learning from nature to develop new concepts for cell preservation based on mechanisms with which model organisms tolerate desiccation and/or remain metabolically inactive for long periods
Goals
• Reveal strategies employed by different organisms that facilitate their long term survival in a dormant form
• Widening the basis of our knowledge on the molecular details of strategies employed by 5 model organisms
• Resolve whether these strategies can be used to develop preservation (“ reversible dormancy ”) approaches at ambient temperatures for different cells and organisms
* Emphasis on understanding processes that control the establishment and maintenance of dormant stages and the exit from dormancy
Specific goals
WP 1 & 2 Discover the genes, proteins and compounds that are associated with the formation of dormant forms and exit from it in:
CyanobacteriaYeastRotifers
WP 3. Discerning the processes associated with desiccation as a survival strategy by global gene expression, proteome and metabolome profiling
Arctic springtailkillifish embryos
Specific Goals - Continued
WP 4. Confirming the role of specific genes in tolerance to desiccation or dormancy by genetic analyses and genetic genetic engineering experiments
YeastKillifishRotifers (genetically selected strains)
WP 5. Discovering common and divergent strategies
through synthesis of the genomic, proteomic and metabolomic data of model organisms and of data from other organisms, including plants – in silico models
Specific Goals - Continued
• WP 6. Publicity to stir and encourage new research direction
Scientific publications, scientific meetingsWorkshopBook
• Tools: High through approaches; genomics, proteomics, metabolomics and bioinformatics
Partners• Ora Hadas, Assaf Sukenik (IOLR)- Cyanobacteria
• Joan Cerdà, IRTA, Barcelona, Spain - Killifish
• Stefan Hohmann, Goteborg University, Sweden - yeast
• Melody Clark & Roger Worland, BAS, UK – Arctic sprintail
• Richard Reinhardt, Max Planck Institute –Molecular Genetics, Berlin-Dahlem, Germany – Genomic and proteomic platform
• Jens Nielson, Danish Technical University, Lyngby, Denmark -metabolomic platform
WP Number
TitleLeading partner &Participating partners
Startingmonth
WP0ManagementEsther LubzensM0
WP1 Establishment of dormant stages
Esther Lubzens & Ora Hadas- P3, P5, P6
M0
WP2 Arousal from dormancy
Stefan Hohmann – P1, P5, P6
M0
WP3 Desiccation and revival
Joan Cerdà – P4, P5, P6
M0
WP4Engineering /genetic testing
Joan Cerdà – P1, P3, P5, P6
M15
WP5Common and divergent pathways
Stefan Hohmann & Melody Clark – P1, P2, P5, P6
M0
WP6DisseminationEsther Lubzens –P2, P3, P4, P5, P6
M12
WP1 Dormant stages
P1, P3, P5
WP2 Germination and arousal
from dormant stages P1, P3, P5
WP3 Resistance to desiccation
P2, P4, P5
WP4 Engineering /genetic
testing strategies P1, P2, P3
WP5 Identification of common pathways for dormant stages,
revival/germination and resistance to desiccation P1-P6
WP1 Dormant stages P1, P3, P5, P6
WP2 Germination and arousal
from dormant stages P1, P3, P5, P6
WP3 Resistance to desiccation
P2, P4, P5, P6
Towards novel concepts and methods for cell preservation
WP6 Participation in scientific meetings, organization of a
workshop and publication of a book on dormancy and resistance to dessication
P1-P6
Pert Chart for SLEEPING BEAUTY