Dominique Maes

Structural Biology Brussels (SBB) Faculteit Wetenschappen en Bio-ingenieurswetenschappen Vrije Universiteit Brussel (VUB) Building E, room E5.8 Pleinlaan 2 B-1050 Brussel Belgium tel: xx-32-(0)2 629 18 52 fax: xx-32-(0)2 629 19 63 GSM: xx-32-(0)476 68 34 37 email: dominique.maes@vub.ac.be

Huidige situatie: academiejaar 2014-2015

Wiskundige en Fysische modellen: data analyse in de Biotechnologie 3de Bachelor Bio-ingenieurswetenschappen 39 HOC, 26 WPO, 6 ECTS Mathematics and Statistics Interuniversity Program in Molecular Biology: Master Molecular Biology 26 HOC, 3 ECTS Meerdimensionale statistiek 3de Bachelor Geografie + keuzevak 3de Ba Biologie 26 HOC, 26 WPO, 6 ECTS Biostatistics Master Biomolecular Sciences 26 HOC, 52 ZELF, 5 ECTS Calculus 1 1ste Bachelor Computerwetenschappen (miv. schakels + avondstudenten), Geografie, Scheikunde en Biologie 30 HOC, 30 WPO, 4 avondclinics, 6 ECTS Kansrekening en statistiek 2de Bachelor Informatica, Bio-ingenieur, Geografie, Scheikunde en Biologie 26 HOC, 26 WPO, 6 ECTS Inleiding Statistiek 2de Bachelor Wiskunde 13 HOC, 13 WPO, 3 ECTS

Overzicht vanaf aanstelling

Mathematical Concepts in Molecular Biology Postgraduate: Tropical Molecular Biology Periode 1990-1992 Toegepaste Wiskunde 1° Graad Bio-ingenieur Periode 1993-1994 : interim Wiskundige en Fysische modellen: data analyse in de Biotechnologie 3de Bachelor Bio-ingenieurswetenschappen (1° Graad Bio-ingenieur) Periode 1994-heden Mathematical Concepts in Molecular Biology Interuniversity Program in Molecular Biology Periode 1999-2005

Mathematics and Statistics Interuniversity Program in Molecular Biology: Master Molecular Biology Periode 2006-heden Data analyse en numerieke methoden 2de Bachelor (2de kandidatuur) Bioingenieur Periode 2001- (vanaf 2010 Kansrekenen en Statistiek) Toegepaste Wiskunde 1 1ste kandidatuur Informatica Periode 2003-2004: (partim in collaboration with P. Cara) Kansrekening en statistiek 2de Bachelor (2de kandidatuur) Informatica, Bio-ingenieur, Geografie, Scheikunde en Biologie Periode 2004-heden Inleiding Statistiek 2de Bachelor Wiskunde Periode 2010-heden Meerdimensionale statistiek 3de Bachelor (1ste licentie) Geografie + keuzevak 3de Ba Biologie Periode 2004-heden Wiskunde voor informatici 1 1ste Bachelor Informatica Periode 2004-2010 Calculus 1 1ste Bachelor Computerwetenschappen (miv. schakels + avondstudenten), Geografie, Scheikunde en Biologie Periode 2010-heden Biostatistics Master Biomolecular Sciences (2008-2012: keuzevak met telkens ingeschrevenen, vanaf 2012 verplicht) Periode 2008-heden

Onderwijsevaluaties De onderwijsevaluaties zijn steeds goed tot zeer goed. Tijdens het academiejaar 2013-2014 heb ik van de biologiestudenten ‘De Gouden Spons’ gekregen als ‘beste prof’. Ik beschouw dit als een mooie appreciatie van mijn didactische ‘kwaliteiten’; wiskunde is meestal niet zo’n populair vak voor deze groep van studenten.

Administratieve en Beleidstaken binnen de

universiteit

Niveau Universiteit

- Lid van de Raad van bestuur van het VUB-ULB Rekencentrum 1997 - 2011. - Lid van de AP Beroepscommissie 2013 – heden. - Waarnemer, afgevaardigd door de RvB, in de VUB-Foundation – Fundraising Board 2015 – heden. - Lid van de verkiezingscommissie van rector 2015

Niveau Faculteit

- Ombudsvrouw van de Faculteit WE 2011 – heden. - Lid van de Beroepscommissie van de Faculteit, 2012 – heden. - Lid van de Facultaire Gendercommissie 2014 – heden. - Lid van de “diploma uitreiking commissie” 2010 – heden. - Lid van de Facultaire PR-commissie 2012 – heden.

Onderwijs gerelateerd

- Secretaris van de Examencommissie Bachelor bio-ingenieurswetenschappen. - Lid van de Opleidingsraad Biologie. - Lid van de Opleidingsraad Geografie. - Lid van de Opleidingsraad Computerwetenschappen. - Lid van de Bachelor Examencommissie Chemie. - Lid van de Bachelor Examencommissie Computerwetenschappen. - Lid van de Bachelor Examencommissie Biologie. - Lid van de Bachelor Examencommissie Geografie. - Lid van de Bachelor Examencommissie Bio-ingenieur. - Lid van de Examencommissie Master in Biomolecular Sciences. - Lid van de Examencommissie Master Molecular Biology. Vanaf het academiejaar 2015-16 ben ik geen lid meer van de examencommissie vanwege een mogelijk conflict met mijn taak als ombudsvrouw

Niveau Vakgroep

- Verantwoordelijk voor het tweejaarlijks verslag van het Instituut voor Moleculaire Biologie 1992 -1992 en 1993 - 1994. - Secretaris van de Raad van het Instituut voor Moleculair Biologie, 1994. - Secretaris van het department biotechnologie, 2000 - 2009. - Lid van het dagelijks Bestuur van het department biotechnologie, 2007 - 2009. - Verantwordelijke voor de website ‘bio-ingenieur.be’, 2007 - 2014.

Onderzoek

Projecten Design of cryo-crystallography. (co-promoter) Period: 01/10/1997 - 31/12/1998 Budget: 14873.61 euro, Financing: OZR Molecular physiology: investigations on the strategies of adaptation to high and low temperatures in carbamoyltransferases and carbamoylphosphate synthetases. (co-promoter) Period: 01/01/1999 - 31/12/1999 Budget: 66931.25 euro, Financing: Min. Nat. Lot. (ikv FWO toek) Molecular physiology: investigations on the strategies of adaptation to high and low temperatures in carbamoyltransferases and carbamoylphosphate synthetases. (co-promoter) Period: 01/01/1999 - 31/12/2002 Budget: 467713.34 euro, Financing: FWO Moleculair Basis of Archaeal Physiology: The Bipolar Pyrimidine Operon and the Leucine/Lrp Regulon of Sulfolobus Acidocaldarius as Modelsystems for the Analysis of Mechanisms of Specific and Global Gene Regulation. (co-promoter) Period: 01/01/2000 - 31/12/2003 Budget: 154256.30 euro, Financing: FWO Arginine regulon in yeast: 1) mechanism of transcription initiation from a TATA-LESS promotor: 2) structural studies of enzymes activation by autoproteolysis 3) research on a mitochrondrial metabolon. (co-promoter) Period: 01/01/2001 - 31/12/2004 Budget: 247298.60 euro, Financing: FWO Multifunctional transcriptionregulators of the carbamoylfosfatesynthetase operon carAB in Escherichia coli: procedure and source. (co-promoter) Period: 01/01/2002 - 31/12/2005 Budget: 165298.37 euro, Financing: FWO Transcriptieregulatie bij Archaea : Lrp-actige transcriptieregulatoren bij het hyperthermo-acidofiele crenarchaeon Sulfolobus solfataricus P2. (co-promoter) Period: 01/01/2003 - 31/12/2006 Budget: 14873.66 euro, Financing: IWT- Basale en gereguleerde transcriptie-initiatie by hyperthermofiele Archaea : fysiologie en moleculair mechanisme van Lrp-achtige transcriptieregulators en van pyrimidine-specifieke regulatie by Sulfolobus. (co-promoter) Period: 01/01/2004 - 31/12/2007 Budget: 168.000 euro, Financing: FWO Belgian Principal Investigator of ESA project (2006-2008) Influence of mass transport and surface growth processes on protein crystal perfection ESA- AO2004_070

Budget: 520.000 euro, Financing: ESA-PRODEX Belgian Principal Investigator of ESA project (2009-2011) Influence of mass transport and surface growth processes on protein crystal perfection ESA- AO2004_070 Budget: 825.000 euro, Financing: ESA-PRODEX Principal Investigator of ESA project (2012-2013) Influence of mass transport and surface growth processes on protein crystal perfection ESA- AO2004_070 Budget: 351.750 euro, Financing: ESA-PRODEX Real-time high-throughput (sub-)THz lab-on-chip systems for the label-free monitoring of biomolecular interactions (co-promoter) Period: 01/01/2013 – 31/12/2016 Budget: 240.000 euro, Financing: FWO Principal Investigator of ESA project (2014-2015) Influence of mass transport and surface growth processes on protein crystal perfection ESA- AO2004_070 Budget: 455.700 euro, Financing: ESA-PRODEX Co-Investigator of NASA project (2014-2018) Solution convection and the nucleation precursors in protein crystallization NNH13ZTT001N: 13-13CFStep2-0020 Promotor of Francqui International Professor (sept 2014-aug 2016) Prof Peter G. Vekilov, University of Houston Budget: 41.000 euro, Financing: Francqui Stichting

Lidmaatschappen en bestuurfuncties van internationale organisaties Member of the European Union Round Table on Synchrotrons (Large Scale Facilities) period 1992-1993 European Synchrotron Radiation Society (ESRS) Council Member : period 1994 Executive Member : period 1995 - 2001 Secretary : period 1998 - 2001 Member of “Nationaal Comite voor Kristallografie van de Koninklijke Vlaamse Academie van Belgie voor wetenschappen en kunsten”: 2000 - present Member of the Board of the “Nederlandse vereniging voor Kristallografie”: 2007 – 2012 Council member of the “International Organisation of Crystal Growth (IOCG)”: 2010 - present Coordinator of the ESA-International Topical Team on Crystallization: 2012 – present Council Member of International Organization for Biological Crystallization: 2014 - present

Awards European Space Agency Team Achievement Award: “In recognition of your outstanding contribution and with special appreciation for your professional dedication to the successful Foton-M3 Mission: 14-26 September 2007”

PhD’s Als co-promotor Sigrid Van Boxstael: Aspartate transcarbamylase of the hyperthermophilic archaeon Pyrococcus abyssi: structural and kinetic studies. 08/03/04 Neel Devroede: Mechanisms of purine- and pyrimidine-dependent repression of the Escherichia coli carAB operon, encoding carbamoyl phosphate synthase. 20/09/06 Eveline Peeters: Ss-LrpB, a novel transcriptional regulator from Sulfolobus solfataricus P2. 25/04/07 Phu Nguyen Le Minh Mechanisms of transcriptional gene regulation in Escherichia coli: the carbamoylphosphate synthase operon carAB and genes of arginine transport. 10/06/10 Als Promotor Mike Sleutel: Growth kinetics and mass transport phenomena during protein crystallization. 19/12/08 Rodrigo Gallardo Simultaneous peptide and lipid binding controls membrane recruitment of the scaffolding protein syntenin. 09/03/12 Jacinte Beerten Sequence determinants of short stretch protein aggregation. 20/08/12 Aleksandra Siekierska Molecular effect of mutations on protein aggregation. 6/11/12

Lezingen op uitnodiging “Diffraction Techniques in Biology.” Summer School on Synchrotron Radiation: Applications in Life Sciences and Chemistry, Maratea, IT, 2-10/10/1996. “Protein thermostability: a case study on Triose-phosphate isomerase” EMBL-Heidelberg, Germany, 25/2/98. “Structural studies on Triosephosphate Isomerase” Faltertag; Colloquium on the structure, stability and folding of proteins. University of Regensburg, Germany, 15-17/4/99. “Protein thermostability: a case study on Triose-phosphate isomerase” NVK-Meeting Bio-macromolecular Crystallography, Utrecht, 4/6/99. “Protein Crystallography: More than just structures !” University of Milan, Dipartimento Chimica Strutturale, Milano, Italy, 19/5/00. “As physicist in a biotechnological world!” Limburgs Universitair Centrum, Diepenbeek, België, 19/02/04 “PROMISS, Protein Microscope for the International Space Station” Soyuz Meeting, Toledo, Spain, 26-30/06/06 “Protein Crystallization under microgravity in Europe” 11th International Conference on the Crystallization of Biological Macromolecules (ICCBM11) Quebec, Canada, 16-21/08/2006 “Current status of the microgravity research in Brussels” Topical Team Meeting: ESTEC, Noordwijk, 19-20/10/06 “Towards a definition of crystal quality: X-ray data analysis of the crystals obtained in the PromISS-4 experiment. ISPS2007, Third International Symposium on Physical Sciences in space, Nara, Japan, 21-25/10/07 “Towards a definition of crystal quality” 12th International Conference on the Crystallization of Biological Macromolecules (ICCBM12), Cancun, Mexico, 6-9/5/08 “The Protein Crystallization Diagnostic Facility (PCDF)” ESA-Topical Team Meeting, Brussels, Belgium, 25-26/11/10 “Protein Crystal Growth Studies in Brussels” ESA-JAXA Topical Team Meeting, Brussels, Belgium, 8-9/03/12 “Aggregation of Crystal Packing Fragments” Crystallization of Biological Macromolecules (ICCBM14), Huntsville, Alabama USA, 23-28/11/12 “Are Packing Fragments of Crystals from Biological Macromolecules prone to Aggregation” Collaborative Conference on Crystal Growth (3CG), Orlando, Florida USA, 11-14/12/12

“The Effects of Impurities on Crystal Growth” ESA-JAXA: Topical Team Meeting on Protein Crystal Growth and Colloidal Crystallization, Sendai-Japan, 3-5/03/13 “The effect of impurities and solvent viscosity on protein crystal growth.”, The 28th Meeting of the European Crystallographic Association (ECM28), Warwick, UK, 25-29/08/13 “The study of protein nucleation”, ESA TTM meeting, 27/03/14, Brussels, Belgium. “Nucleation precursors and the birth of crystals”, 15th International Conference on the Crystallization of Biological Macromolecules (ICCBM15), Hamburg, Germany, 17-20/9/14 “Nucleation in Macromolecular Solutions”, International Topical Team Workshop and ESA's "Colloidal Solids" instrument, (ITTM), ESTEC, Noordwijk, Nederland, 5-7/11/14 “Protein clusters and crystals”, Workshop: "Directional Nucleation and Growth of Molecular Crystals", International Solvay Institutes of Physics and Chemistry, ULB, Brussels, Belgium, 26-28/11/14 “Protein rich clusters and crystals at birth”, Francqui Symposium, Brussels, Belgium, 4-6/06/15 “Protein clusters and crystals at birth”, The 20th American Conference on Crystal Growth and Epitaxy (ACCGE-20), and the 17th U.S. Biennial Workshop on Organometallic Vapor Phase Epitaxy (OMVPE-17), Big Sky, Montana, USA, 2-7/08/15 “Protein rich clusters and crystals in microgravity”, The 6th International Symposium of Physical Sciences in Space (ISPS-6 and ITTW2015 Joint Conference), Kyoto, Japan, 14-18/09/15 "Does Microgravity Improve the Quality of Protein Crystals?”, co-chair of panel discussion, 6th International Symposium on Physical Sciences in Space (ISPS-6 and ITTW2015 Joint Conference), Kyoto, 14-18/09/15 “How camels can aid crystallization? The challenges of the de novo designed protein Octarellin” 16th International Conference on the Crystallization of Biological Macromolecules (ICCBM16), Prague, Czech Republic, 2-7/07/16 “Classical and two-step nucleation of glucose isomerase”, 18th International Conference on Crystal Growth and Epitaxy (ICCGE-18), Nagoya, Japan, 7-12/08/16

Organisatie van International Meetings: lid van organiserend comité - ESRS Summer School on Synchrotron Radiation: Applications in Life Sciences and Chemistry. Maratea, Italy, (2-10/10/1996). Supported by the EU-TMR Program Co-organizers: Malcolm Cooper (Warwick University, ESRS President) and Lorenzo Avaldi (IMAI-CNR, Roma) - 2nd Belgian Crystallography Symposium 24/10/01, Brussel, Belgium

- 3rd Belgian Crystallography Symposium 22/10/03, Brussel, Belgium - 4th Belgian Crystallography Symposium 19/10/05, Brussel, Belgium - European Crystallographic Meeting (+ member of the Program Committee) 6-11/08/06, Leuven, Belgium - 5th Belgian Crystallography Symposium 15/10/08, Brussel, Belgium - Workshop on: “Kinetics and thermodynamics of multistep nucleation and self-assembly in nanosize materials” Brussels organisator tezamen met G. Nicolis (ULB), March 25-26, 2010 - 6th Belgian Crystallography Symposium 13/10/10, Brussel, Belgium - ESA-Topical Team Meeting 25-26/11/10, Brussels, Belgium - 7th Belgian Crystallography Symposium 10/10/12, Brussel, Belgium - ESA-JAXA Topical Team Meeting 8-9/03/12, Brussels, Belgium - ESA-JAXA: Topical Team Meeting on Protein Crystal Growth and Colloidal Crystallization. 3-5/03/13, Sendai-Japan - ESA: Topical Team Meeting on Protein Crystal Growth and Colloidal Crystallization. 27-28/03/14, Brussels, Belgium - 8th Belgian Crystallography Symposium 15/10/14, Brussel, Belgium - ESA-JAXA: Topical Team Meeting on the Nanostep2 Project 11/03/15, Brussels, Belgium - Francqui Symposium on Aggregation of Biological Molecules 4-6/06/15, VUB, Brussels, Belgium - 9th Belgian Crystallography Symposium 26/10/16, Brussel, Belgium

Publicaties in Internationale wetenschappelijke tijdschriften met

leescomité C. Van den Broeck and D. Maes Retention and peak width in Field Flow Fractionation with wall effects. Sep. Sci. 22(4), 1269-1280, 1987. C. Van den broeck, D. Maes and M. Bouten Lower bounds for the asymptotic rate of dispersion in generalized Taylor diffusion. Phys. Rev. A36, 5025-5030, 1987. D. Maes and C. Van den Broeck Discrete versus continuous time random walks. J. Stat. Phys. 50, 1089-1102, 1988. D. Maes and C. Vanderzande Self-avoiding rings at the theta point. Phys. Rev. A41, 3074-3079, 1990. D. Maes and C. Van den Broeck Field Flow Fractionation for Poiseuille flow through a cylindrical tube. Sep. Sci. 26(10-11), 1341-1352, 1991. D. Maes, L. Wyns, J. Lisgarten, D. Lisgarten and R. Palmer Study of (20R)-5α-pregnano [3,4-C] [1,2,5] oxadiazole-20-ol (HS1011). Acta Cryst. C48, 1092-1095, 1992. D. Maes, P. Fiddelaers, L. Wyns, J. Lisgarten, D. Lisgarten and R. Palmer Structure of (17α)-androstano [3,4-C] [1,2,5] oxadiazole-17-ol (HS963). Acta Cryst. C48, 1954-1957, 1992. R. Loris, J. Lisgarten, D. Maes, R. Pickersgill, F. Körber, C. Reynolds and L. Wyns Two crystal forms of the lentil lectin diffract to high resolution. J. Mol. Biol. 223, 579-581, 1992. D. Maes, M. Van Cauteren, L. Wyns, J. Lisgarten, R. Palmer, D. Lisgarten, R. Willem, M. Biesemans and F. Kayser The molecular structure of [1,2,5]oxadiazolo[3',4':3,4]-5α-pregn-16-en-20-one (HS974); crystal structure and NMR investigations. J. Chem. Soc. Perkin Trans. 2, 2179-2185, 1992. C. Dealwis, R. Janes, R. Palmer, J. Lisgarten, D. Maes, C. Flint and D. Majaha Gazi Structures of chromium (III) cyclam complexes 3: Structure of trans-dichloro (1,4,8,11-tetraozacyclotetradecane) chromium (III) bromide. Acta Cryst. C48, 1754-1756, 1992. R. Loris, J. Steyaert, D. Maes, J. Lisgarten, R. Pickersgill and L. Wyns Crystal Structure determination and refinement at 2.3-Å resolution of the lentil lectin. Biochemistry 32, 8772-8981, 1993.

J. Lisgarten, V. Gupta, D. Maes, L. Wyns, I. Zegers, R.A. Palmer, C. Dealwis, C.F. Aguilar and A. Hemmings Structure of the crystalline complex of cytidylic acid (2'-CMP) with ribonuclease at 1.6 Å resolution. Conservation of solvent sites in RNase-A High-Resolution structures. Acta Cryst. D49, 541-547, 1993. D. Lisgarten, R. Palmer, D. Maes and J. Lisgarten The structure of 20 oximo-5α-pregnano-16-eno[3,4-C]- 1',2','5'-oxadiazole (HS976). J. Cryst. Spect. Res. 23, 359-366, 1993. K. Decanniere, D. Maes, J. Lisgarten, I. Zegers and M. Biesemans The structure of 23,24-dinor-5-α-cholan-12-one. Acta Cryst. C49, 1824-1826, 1993. J. Bouckaert, D. Maes, J. Lisgarten, T. Chattopadhyay, R. Palmer, M. Mazid and D. Gazi Structures of chromium (III) cyclam complexes 5: Structure of trans-dichloro-(1,4,8,11-tetraazacyclotetradecane)-chromium (III) isothiocyanate. Acta Cryst. C49, 1361-1363, 1993. R. Loris, D. Maes, J. Lisgarten, M. Bettinelli and C. Flint Crystal and molecular structure of Bis(tetramethyl-ammonium) hexachlorenate (IV). Acta Cryst. C49, 231-233, 1993. I. Zegers, D. Maes, M.H. Dao Thi, R. Palmer and L. Wyns The structures of RNase A complexed with 3'-CMP and d(CpA): active site conformation and conserved water molecules. Protein Science 3, 2322-2339, 1994. M. Gielen, A. El Khloufi, M. Biesemans, F. Kayser, R. Willem, B. Mahieu, D. Maes, J. Lisgarten, L. Wyns, T. Chattopadhay and R. Palmer Tri-n-butylin 2,6-difluorobenzoate, a unique macrocyclic tetramer containing a sixteen-membered Sn4C4O8 ring, and other related compounds. Organnometallics 13,2849-2854, 1994. P. Verheyden, H. Jaspers, J. Martins, D. Maes, J. Pletinckx, G. Vanbinst, L. Wyns and R. Willem H-1-NMR study of the structure of Ac-AMP2, a peptide isolated from Amaranthus caudatus Arch. Int. Phys. Biochim. Biophys. 102, B43-B43, 1994. D. Lisgarten, R. Palmer, D. Maes, J. Lisgarten and L. Wyns Two steroidal epimers, (5,17) pregna-20yno[3,4-C](1,2,5)-oxadiazole-17-ol (the 5-epimer) and (5,17-pregna-20-yno[3,4-C](1,2,5)oxadiazole-17-ol(the 5-epimer). Acta Cryst. C51, 666-770, 1995. J. Lisgarten, D. Maes, L. Wyns, C. Aguilar and R. Palmer Structure of the crystalline complex of deoxycytidylyl-3',5'-guanosine (3',5'-dCpdG) co-crystalised with ribonuclease at 1.9Å resolution. Acta Cryst. D51, 767-771, 1995. F. Kayser, D. Maes, L. Wyns, J. Lisgarten, R. Palmer, D. Lisgarten, R. Willem, J.C. Martins, P. Verheyden and M. Biesemans A comparative structural study on the steroids [1,2,5] oxadiazolo [3’,4’/3,4]-5-alpha-pregn-16-en-20-one oxime (HS998), [1,2,5] oxadiazolo [3’,4’/3,4]-5-alpha-pregn-16-en-20-one (HS974), and [1,2,5]

oxadiazolo [3’,4’/3,4]-5-beta-pregn-16-en-20-one (HS973) by NMR, molecular modeling and X-ray investigations. Steroids 60, 713-719, 1995. P. Verheyden, J. Pletinckx, D. Maes, H. A. M. Pepermans, L. Wyns, R. Willem and J. C. Martins H NMR study of the interaction of N, N’, N’’-triacetyl chitotriose with Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus. FEBS Letters 370, 245-249, 1995. M. Azarkan, D. Maes, J. Bouckaert, M. H. Dao Thi, L. Wyns and Y. Looze Thiol pegylation facilitates purification of chymopapain leading diffraction studies at 1.4 Å resolution Journal of Chromatography A749, 69-72, 1996. R. Loris, D. Maes, F. Poortmans, L. Wyns and J. Bouckaert A structure of the complex between Concanavalin A and methyl-3,6-di-O-(-D-mannopyranosyl)-D-mannopyranoside reveals two binding modes. J. Biol. Chem. 271, 30614-30618, 1996. J. Martins, D. Maes, R. Loris, H. Pepermans, L. Wyns, R. Willem and P. Verheyden 1H NMR study of the solution of Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus. J. Mol. Biol 258, 322-333, 1996. J. N. Lisgarten, D. Maes, M. H. Dao Thi, L. Wyns, D. L. Key and J. A. Marks Crystal orientation effect under microgravity conditions. J. Cryst. Growth 163, 474-477, 1996. D. Maes, J. Bouckaert, F. Poortmans, L. Wyns and Y. Looze Structure of chymopapain at 1.7 Å resolution. Biochemistry 35, 16292-16298, 1996. M. Alvarez, J. Ph. Zeelen, V. Mainfroid, F. Rentier-Delrue, J.A. Martial, L. Wyns, R.K. Wierenga and D. Maes Triose-phosphate Isomerase (TIM) of the Psychrophilic Bacterium Vibrio marinus : kinetic and structural properties. J. Biol. Chem 273,2199-2206, 1998. A.Baeten, D. Maes and P. Geerlings Quantumchemical study of the catalytic triad in subtilisin: the influence of amino acid substitutions on enzymatic activity. J. Theoret. Biol., 195, 27-40, 1998. U. Langhorst, R. Loris, V.P. Denisov, J. Doumen, P. Roose, D. Maes, B. Halle and J. Steyaert Dissection of the structural and functional role of a conserved hydration site in RNase T1. Protein Sci., 8, 722-730, 1999. R. Loris, U. Langhorst, S. De Vos, K. Decanniere, J. Bouckaert, D. Maes, T.R. Transue and J. Steyaert Conserved water molecules in a large family of microbial ribonucleases. Proteins, 36, 117-134, 1999. R. Cunin, F. Van Vliet and D. Maes

Active site residues involved in co-operativity and allosteric regulation in the aspartate transcarbamylases from Escherichia coli and from teh hyperthermophilic archaeon Pyrococcus abyssi. FASEB J, 13, A1529-1529, 1999. M. Alvarez, J. Wouters, D. Maes, V. Mainfroid, F. Rentier-Delrue, L. Wyns, E. Depiereux and A. Martial Lys13 plays a crucial role in the functional adaptation from the thermophilic TIM of Bacillus stearothermophilus to high temperatures. J. Biol. Chem., 274, 19181-19187, 1999. D. Maes, J. Ph. Zeelen, N. Thanki, N. Beaucamp, M. Alvarez, M. H. Dao Thi, J. Backmann, J. A. Martial, L. Wyns, R. Jaenicke and R. K. Wierenga The crystal structure of Triosephosphate Isomerase (TIM) from Thermotoga maritima: A comparative thermostability structural analysis of ten different TIM structures. Proteins 37, 441-453, 1999. D. Maes and J. Backmann Protein thermostability: a case study on triosephosphate isomerase. Recent Res. Devel. Biochem., 1, 105-116, 1999. J. Wouters and D. Maes Identification of a potential metal cation- binding site in the structure of a thermophilic Bacillus stearothermophilus triosephosphate isomerase mutant. Acta Cryst. D56, 1201-1203, 2000. V. Pector, J. Backmann, D. Maes, M. Vandenbranden and J. Ruysschaert Biophysical and Structural Properties of DNA·diC14-amidine Complexes. J. Biol. Chem., 275, 29533-29538, 2000. D. Charlier, A. Kholti, D. Gigot, D. Maes, TL. Thia-Toong and N. Glansdorff Mutational analysis of Escherichia coli PepA, a multifunctional DNA-binding aminopeptidase. J. Mol. Biol., 302, 409-424, 2000. K. Decanniere, T.R. Transue, A. Desmyter, D. Maes, S. Muyldermans and D. Maes Degenerate interfaces in Antigen-Antibody complexes. J. Mol. Biol., 313, 473-478, 2001. M.H. Dao-Thi, D. Charlier, R. Loris, D. Maes, J. Messens, L. Wyns and J. Backmann Intricate interactions within the ccd plasmid addiction system. J. Biol. Chem., 277, 3733-3742, 2002. S.Van Boxstael, R. Cunin, S. Khan and D. Maes Aspartate Transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi: Thermostability and 1.8 Å resolution crystal structure of the catalytic subunit complexed with the bisubstrate analogue N-phosphonacetyl-L-aspartate. J. Mol. Biol., 326, 203-216, 2003. N. Devroede, T.T. Thia-Lin, D. Gigot, D. Maes and D. Charlier Purine and pyrimidine-specific repression of the Escherichia coli carAB operon are functionally and structurally coupled. J. Mol. Biol., 336, 25-42, 2004.

D. Maes, L.A. Gonzalez-Ramirez, J. Lopez-Jaramillo, B.Yu, H. De Bondt, I. Zegers, E. Afonina, J.M. Garcia-Ruiz and S. Gulnik Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae. Acta Crystallographica D60, 463-471, 2004. E. Peeters, T-L. Thia-Toong, D. Gigot, D. Maes and D. Charlier Ss-LrpB, a novel Lrp-like regulator of Sulfolobus solfataricus P2, binds cooperatively to three conserved targets in its own control region. Mol. Microbiol., 54(2), 321-336, 2004. S. Van Boxstael, D. Maes and R. Cunin Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi : insights into cooperative and allosteric mechanisms FEBS J., 272, 2670-2683, 2005. E. Peeters, Bach Thi Mai Hoa, I. Zegers, D. Charlier and D. Maes Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of Ss-LrpB, a transcription regulator from Sulfolobus solfataricus. Acta Cryst., F61, 985-988, 2005. J. Massant, E. Peeters, D. Charlier and D. Maes Crystallization and preliminary X-ray diffraction analysis of the arginine repressor of the hyperthermophile Thermotoga neapolitana Acta Cryst., F62, 26-28, 2006. E. Peeters, R. Willaert, D. Maes and D. Charlier Ss-LrpB from Sulfolobus solfataricus condenses about 100 basepairs of its own operator DNA into globular nucleoprotein complexes J. Biol. Chem., 281, 11721-8, 2006 D. Tysmans, P. Claeys, L. Deriemaeker, D. Maes, R. Finsy and M. Van Molle Size and shape analysis of Sedimentary grains by Automated Dynamic Image Analysis Particle and Particle System Characterization, 5 vol 23, 381-387, 2006 D. Maes, M. Crabeel, C. Van de Weerdt, J. Martial, E. Peeters, D. Charlier, C. Vanhee, L. Wynsa and I. Zegersa Crystallisation of ornithine acetyl transferase from yeast by counterdiffusion Acta Cryst., F62, 1294-1297, 2006 E. Peeters, C. Wartel, D. Maes and D. Charlier Analysis of the DNA-binding sequence specificity of the archaeal transcriptional regulator Ss-LrpB from Sulfolobus solfataricus by systematic mutagenesis and high resolution contact probing Nucleic Acid Research, 35, 623-633, 2007 D. Maes, K. Decanniere, I. Zegers, C. Vanhee, M. Sleutel, R. Willaert, C. Van De Weerdt, J. Martial, JP. Declercq, C. Evrard, F. Otalora and JM. Garcia-Ruiz. Protein crystallisation under microgravity conditions: What did we learn on TIM crystallisation from the Soyuz missions ? Microgravity Science and Technology, XIX-5/6, 90-94, 2007 C. Evrard*, D. Maes*, I. Zegers, JP. Declercq, C. Vanhee, J. Martial, L. Wyns and C. Van De Weerdt * equal contribution

TIM crystals grown by capillary counter-diffusion: Statistical evidence of quality improvement in microgravity. Crystal Growth and Design, 7, 2161-2166, 2007. M. Sleutel, C. Vanhee, C. Van de Weerdt, K. Decanniere, D. Maes, L. Wyns and R. Willaert The Role of Surface Diffusion in the Growth Mechanism of Triosephosphate Isomerase Crystals Crystal Growth and Design, 8, 1173-1180, 2008. M. Sleutel, C. Vanhee, I. Zegers, L. Wyns, D. Maes and R.Willaert Kinetic Roughening of Glucose Isomerase Crystals Crystal Growth and Design, 8, 4409-4414, 2008. D. Maes, C. Evrard, JA. Gavira, M. Sleutel, C. Van De Weerdt, F. Otalora, JM Garcia-Ruiz, G. Nicolis, J. Martial and K. Decanniere. Towards a definition of X-ray crystal quality. Crystal Growth and Design, 8, 4284–4290, 2008. V. Basios, J. Lutsko, G. Nicolis, D.Maes and C. Kirschhock A new paradigm of crystallization arising from non-standard nucleation pathways Microgravity Research and Technology, 21-1/2, 47-51, 2009. M. Sleutel, R. Willaert, L. Wyns and D. Maes Polyhedral instability of protein crystals Crystal Growth and Design, 9, 335-343, 2009. M. Sleutel, R. Willaert, C.M. Gillespie, C. Evrard, L. Wyns and D. Maes Kinetics and thermodynamics of glucose isomerase crystallization Crystal Growth and Design, 9, 497-504, 2009. P. Nguyen Le Minh, N. Devroede, J. Massant, D. Maes and D. Charlier Insights into the architecture and stoichiometry of Escherichia coli PepA•DNA complexes involved in transcriptional control and site-specific DNA recombination by atomic force microscopy Nucleic Acid Research, 37, 1463 – 1476, 2009. G. Falony, K. Lazidou, A. Verschaeren, S. Weckx, D. Maes and L. De Vuyst In vitro kinetic analysis of fermentation of prebiotic inulin-type fructans by Bifidobacterium species reveals four different phenotypes Appl. Eviron. Microbiol., 75, 454-461, 2009 J. F. Lutsko, V. Basios, G. Nicolis, J. Kozak, M. Sleutel and D. Maes Kinetic roughening-like transition with finite nucleation barrier J. Chem. Phys., 132, 035102, 2010. This paper is also selected for 1. the January 2010 issue of JCP: BioChemical Physics 2. the February 2010 issue of the Virtual Journal of Biological Physics Research P. Nguyen Le Minh, I. Bervoets, D. Maes and D. Charlier The protein-DNA contacts in RutR•carAB operator complexes Nucleic Acid Research, 38, 6286-6300, 2010. IF=7.479 S. Weckx, R. Van der Meulen, D. Maes, I. Scheirlinck, G. Huys, P. Vandamme and L. De Vuyst

Lactic acid bacteria community dynamics and metabolite production of rye sourdough fermentation share characteristics of wheat and spelt sourdough fermentations Food Microbiology, 27, 1000-1008, 2010. T. Rimaux, G. Vrancken, V. Pothakos, D. Maes, L. De Vuyst, F. Leroy The kinetics of the arginine deiminase pathway in the meat Lactobacillus sakei CTC 494 are pH-dependent Food Microbiology, 28, 597-604, 2011. M. Sleutel, D. Maes and A.E.S. Van Driessche What can mesoscopic level in situ observations teach us about kinetics and thermodynamics of protein crystallization? Adv. Chem. Phys., 151, 223-276, 2012. M. Sleutel, A.E.S. Van Driessche, W. Pan, E. Reichel, K. Decanniere, D. Maes and P.G. Vekilov Does Solution Viscosity Scale the Rate of Aggregation of Folded Proteins? JCPL, 10, 1258-1263, 2012. M. Sleutel, A.E.S. Van Driessche, and D. Maes An Unconventional Mode of Protein Crystal Growth: Case Study Xylanase Crystal Growth and Design, 6, 2986-2993, 2012. L.D. Patino-Lopez, K. Decanniere, J.A. Gavira, D. Maes and F. Otalora Protein Experiment: Scientific Data Processing Platform for On-Flight Experiment Tuning Microgravity Sci. Technol., 24 , 327-334 , 2012. A. Rivière, F. Moens, M. Selak, D. Maes, S. Weckx and L. De Vuyst The ability of bifidobacteria to degrade arabinoxylan oligosaccharide constituents and derived oligosaccharides is strain dependent Appl. Environ. Microbiol., 80(1), 204-217, 2014. DOI: 10.1128/AEM.02853-13. H. Liu, A. Orell, D. Maes, M. van Wolferen, A. Lindås, R. Bernander, S. Albers, D. Charlier and E. Peeters BarR, an Lrp-type transcription factor in Sulfolobus acidocaldarius, regulates an aminotransferase gene in a β-alanine responsive manner Mol Microbiol. 92, 625-639, 2014. DOI: 10.1111/mmi.12583. J.F. Lutsko, N. González-Segredo, M.A. Durán-Olivencia, D. Maes, A.E.S. Van Driessche and M. Sleutel Crystal Growth Cessation Revisited: The Physical Basis of Step Pinning Cryst Growth and Design, 14, 2014. DOI: 10.1021/cg501307y. M. Sleutel, J. Lutsko, A.E.S. Van Driessche, M.A. Duran-Olivencia and D. Maes Observing classical nucleation theory at work by monitoring phase transitions with molecular precision Nature Communication, 5:5598, | DOI: 10.1038/ncomms6598, 2014. M.A. Vorontsova, D. Maes and P.G. Vekilov Recent advances in the understanding of two-step nucleation of protein crystals Faraday Discuss., 2015. DOI: 10.1039/C4FD00217B. P.N.L. Minh, S.d. Sima, I. Bervoets, D. Maes, V. Rubio and D. Charlier

Ligand binding specificity of RutR, a member of the TetR family of transcription regulators in Escherichia coli FEBS Open Bio, 2015. DOI: http://dx.doi.org/10.1016/j.fob.2015.01.002. D. Maes, M.A. Vorontsova, M.A.C. Potenza, T.Sanvito, M. Sleutel, M. Giglio and P.G. Vekilov Do Protein Crystals Nucleate within Dense Liquid Clusters? Acta Cryst., F71, 815, 2015. M. Sleutel, J.F. Lutsko, D. Maes, and A.E.S. Van Driessche Mesoscopic Impurities Expose a Nucleation-Limited Regime of Crystal Growth Phys. Rev. Lett. 114, 245501, 2015. M.A.C. Potenza, T. Sanvito, F.P. Mariani, P.G. Vekilov, D. Maes Confocal Depolarized Dynamic Light Scattering: a Novel Technique for the Characterization of Nanoparticles in Complex Fluids. Archives of Medical Research, 2, 9, 2015. J.F. Lutsko, A.E.S. Van Driessche, M.A. Durán-Olivencia, D. Maes, and M. Sleutel Step Crowding Effects Dampen the Stochasticity of Crystal Growth Kinetics Phys. Rev. Lett., 116, 015501, 2016 M. Sleutel, J. Lutsko, D. Maes and A.E.S. Van Driessche A new class of impurities exposes a nucleation-limited regime of crystal growth PRL The unexpected structure of the designed protein Octarellin V.1 forms a challenge for protein structure prediction tools. M. Figueroa, M. Sleutel, M. Vandevenne, G. Parvizi, S. Attout, O. Jacquin, J. Vandenameele, A.W. Fischer, C. Damblon, E. Goormaghtigh, M. Valerio-Lepiniec, A. Urvoas, D. Durand, E. Pardon, J. Steyaert, P. Minard, D. Maes, J. Meiler, A. Matagne, J. Martial, C. Van de Weerdt J Struct Biol., 195, 19, 2016. doi: 10.1016/j.jsb.2016.05.004. M.A. Vorontsova, P.G. Vekilov and D. Maes Characterization of the diffusive dynamics of particles with time-dependent asymmetric microscopy intensity profiles Soft Matter, 12, 6926-6936, 2016. DOI: 10.1039/C6SM00946H Edited Book Volume G. Nicolis and D. Maes Kinetics and thermodynamics of multistep nucleation and self-assembly in nanosize materials Adv. Chem. Phys. 151, 2012.