Do-NOW!!

1. Strawberries are not diploid, they are octoploid. Why might this make it easier for us to see strawberry DNA than human DNA?

2. After reading the procedure, identify the role of soap, salt, and strawberry “mushing” in the experiment.

3. To refresh your memory, describe the structure of a DNA molecule.

Lab Procedure: DNA Extraction from Strawberries

• Safety:1) Careful with all glassware2) Safety glasses?

Procedures (continued)

• Answer pre-lab questions BEFORE beginning lab

• Changes to Materials Section:1) coffee filter (not cheesecloth)2) pipette (not glass rod)3) teacher will provide small amount of ethanol (not 20 mL)

Procedure• Mush strawberry in ziploc

bag• Add 10 mL of extraction

buffer• Mush again • Filter strawberry slush

through coffee filter• The teacher will pour cold

ethanol into the test tube• Make observations!• Dip pipette into liquid and

observe what happens

Set-Up and Clean-Up

• Where should I get my materials?

• How should I clean up?

• What should I do afterwards?