dna based biosensors
DESCRIPTION
DNA Based Biosensors. Yingli Fu Biological Resources Engineering University of Maryland, College Park December 10, 2003. Outline. Introduction Principles of DNA biosensors Types of DNA biosensors Improvement of DNA biosensors DNA biosensor miniaturization References. Introduction. - PowerPoint PPT PresentationTRANSCRIPT
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DNA Based Biosensors
Yingli Fu
Biological Resources Engineering University of Maryland, College Park
December 10, 2003
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Outline
Introduction
Principles of DNA biosensors
Types of DNA biosensors
Improvement of DNA biosensors
DNA biosensor miniaturization
References
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IntroductionBiosensor:
DNA biosensor:
Motivated by the application to clinical diagnosis and genome mutation detection
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DNA Structure
DNA structures---double helix (complementary) 4 bases:
Adenine (A), Guanine (G),
Thymine (T), and Cytosine (C) sugar (deoxyribose) phosphate group
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DNA Stability
Hydrogen bonding between base pairs
Stacking interaction between bases along axis of double-helixSize and base content and sequence
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Principles of DNA biosensors
Nucleic acid hybridization ---rennealing b/w the ssDNAs from different sources
Perfect match ---stable dsDNA, strong hybridization
One or more base mismatches ----weak hybridization
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Forms of DNA Biosensors
Electrodes
Chips
Crystals
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Immobilization of DNA Probe onto Transducer Surface
Thiolated DNA for self assembly onto gold transducers
Covalent linkage to the gold surface via functional alkanethiol-based monolayers
Use of biotylated DNA for complex formation with a surface-confined avidin or strepavidin
Covalent (carbodiimide) coupling to functional
groups on carbon electrodes
simple adsorption onto carbon surfaces
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Types of DNA Based Biosensors
Optical, Electrochemical and Piezoelectric
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Molecular Beacon Based Optical Fiber DNA Biosensors
‘Ligate and light’. Schematics diagram of real-time monitoring of the nucleic acid ligation process by a MB.
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Piezoelectric DNA Biosensorsquartz crystal microbalance (QCM) transducers
Immobilized DNA probe
Target DNA
Form duplex---mass increase
Decrease in crystal’s resonance frequency
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Piezoelectric DNA Biosensors (cont’s)
Frequency–time response of a PNA/QCM to additions of the target (T) and mismatch (M) oligonucleotides. The hybridization event results in decreased frequency, reflecting the increased mass of the crystal.
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Electrochemical DNA Biosensor
DNA-immobilized electrodes, based on detection of hybridization redox intercalators to recognize dsDNA DNA-mediated electron transfer using mediators Use of ferrocene-labeled oligonucleotide probes that
hybrize to immobilized DNA
Enzyme labels were used to amplify the signal and improve the sensitivity Peroxidase Glucose dehydrogenase (GDH)
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Electrochemical DNA Biosensor---An Example
Amperometric DNA sensor using the pyrroquinoline quinone glucose dehydrogenase-avidin conjugate
Kazunori Ikebukuro, Yumiko Kohiki, Koji Sode *Biosensors and Bioelectronics 17 (2002) 1075--1080
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Material and Methods
pyrroquinoline quinone-dependent glucose dehydrogenase ((PQQ)GDH) for DNA hybridization labeling
Detection via biotin-avidin binding
Target and probe DNA sequence: Target DNA: 5’-bio-TCGGCATCAATACTCATC-3’. Probe DNA: 5’-bio-GATGAGTATTGATGCCGA-3’ Control DNA: 5’-bio-CTGATGAACATACTATCT-3’
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Material and Methods (cont’s)
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Results
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Conclusions
The (PQQ)GDH/avidin conjugate based DNA biosensor is highly sensitive and selective to the target Salmonla invA virulence gene
The sensor response increased with the addition of glucose and in the presence of 6.3 mM glucose the response increased with increasing DNA in the range 5.0x10^8-1.0x10^5
This DNA biosensor would be applicable for single nuleotide polymorphism detection
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Improvement
Fluorescent Bioconjugated Nanoparticles
DNA dendrimers
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Fluorescent Bioconjugated Nanoparticles---signal amplification
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Results
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DNA dendrimers---increase sensitivity
Schematic drawing showing the hybridization detection at the dendrimer/QCM biosensor. The 38-mer probe is attached to the core dendrimer by complementary oligonucleotide (a(-)) binding on one (a(+)) of the outer arms. The probe sequence for target hybridization is 5-GGG GAT CGA AGA CGA TCA GAT ACC GTC GTA GTC TTA AC-3.
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DNA biosensor miniaturization
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Concept of DNA microarray
Figure 2
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DNA microarray
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DNA Microarray (cont’s)
The fluorescence intensities for each spot is indicative of therelative aboundance of the corresponding DNA probe in the Nucleic acid target samples
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The light directed probe array synthesis process used for the preparation of Affymetrix’s Gene Chip
Affymetrix’s Gene Chip
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DNA biosensor not limited to DNA detection, but more…
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References
Kazunori Ikebukuro, Yumiko Kohiki, Koji Sode 2002. Amperometric DNA sensor using the pyrroquinoline quinone glucose dehydrogenase-avidin conjugate. Biosens. Bioelectron. 17,1075—1080
Wang, J. 2000. SURVEY AND SUMMARY From DNA biosensors to gene chips. Nucleic Acids Res. 28(16), 3011-3016
Wang, J., M. Jiang. T. W. Nilsen, R. C. Getts.1998. Dendritic nucleic acid probes for DNA Biosensors. J. AM. CHEM. SOC. 120, 8281-8282
Zhao, X., R. Tapec-Dytioco, and W. Tan. 2003. Ultrasensitive DNA Detection Using highly fluorescent bioconjugated nanoparticles. J. AM. CHEM. SOC. 125, 11474-11475
Zhai, J., H. Cui, and R.Yang. 1997. DNA based biosensors. Biotechnol. Adv.15(1),43-58
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