Developments at the Developments at the Nano/Bio InterfaceNano/Bio Interface

Point to Scientific OpportunitiesPoint to Scientific Opportunities

Developments at the Developments at the Nano/Bio InterfaceNano/Bio Interface

Point to Scientific OpportunitiesPoint to Scientific Opportunities

Functional Molecules and Hybrid Nanostructures

Single Molecule Motion and Complexity

Peptide Complexes Designed for Optoelectronic Function

Saven, DeGrado, Therien, J. Am. Chem. Soc. 2004

Saven, DeGrado, Therien,(2008)

Saven, DeGrado, Therien (2008)

Saven, DeGrado, Therien, Blasie, J. Am. Chem. Soc. 2007

Blasie, DeGrado, Therien, Saven J. Am. Chem. Soc. (2008)

Interface Mediated Behavior in Hybrid Nanostructures

Functional Coxsackie virus-Adenovirus Receptor - Nanotube Hybrid for Detection of Viral Protein (Knob)

Johnson and collaborators, Nano Letters 2007J. Phys. Chem. B 2009

Plasmon Induced Electronic Transport in Functionalized Nanoparticles

Bannerjee et al in pressNanoLetters 08

Ferroelectric Nanolithography Extended to Flexible Substrates

The ultimate goal is to assemble multiple components of diverse properties into complex configurations. Ferroelectric Nanolithography achieves this by controlling local electronic structure on substrates that influences electrontransfer at the surface.

Rankin, et al ACS Nano 2008

NN

N NZn

Ar

Ar

NN

N NZn

Ar

Ar

NN

N NZn

Ar

Ar

SS

300 400 500 600 700 800

0.55

0.60

0.65

0.70

0.75

0.80

Ab

so

rba

nc

e

Wavelength(nm)

400 500 600 700 800 9000.0

0.5

1.0

1.5

2.0

2.5

/

105

M-1

cm

-1

Wavelenghth (nm)

0.0 0.4 0.8 1.2 1.6 2.0 2.4 2.80.0

0.5

1.0

1.5

2.0

2.5

3.0

Cu

rre

nt

(pA

)

Voltage (V)

Dark Red Green Blue

0.00

0.25

0.50

0.75

1.00

0.5

1.0

1.5

2.0

2.5

3.0

Cur

rent

En

ahan

cem

ent

(x1

E-2

8)

Semiconducting Metallic

50 100 150 200 250 3000.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

Temperature (K)

Absorption in the porphyrin Primary and coupled surface plasmons

V- + V- + V- +

Plasmon Induced Electronic Transport in Molecules

Bannerjee, et al in press

Plasmonics Molecular Electronics

SERS single molecule propertieslight guiding switchingcontrol optical properties of organics sensingmeta materialstargeted therapeutics

Harry Atwater, Nature Materials 2003

Mark Reed’s Group

http://www.eng.yale.edu/reedlab/

New mechanism for transduction of optical energy to electrical energy

Optical antenna effect focuses light to the nanoparticle junction increasing absorption efficiency, enhancement can be factors of 105 or more.

Absorption wavelengths/energies can be tuned by choice or design of the molecule and morphological control of nanoparticle array

+

Ghosh, S.K. and T. Pal,. Chemical Reviews, 2007. 107(11): p. 4797-4862

Protein Motion at the Single Molecule Level: Myosin V Processivity

Science 2005, 2007

Goldman Group

Protein Motion at the Single Molecule Level: Myosin V Processivity

Science 2005, 2007

Goldman Group

Protein Motion at the Single Molecule Level: Myosin V Processivity

Science 2005, 2007

Goldman Group

Protein Motion at the Single Molecule Level: Myosin V Processivity

Science 2005, 2007

Goldman Group

Protein Motion at the Single Molecule Level: Myosin V Processivity

Science 2005, 2007

Goldman Group

Medalia et al. 2002 Science. 298:1209-13.

Grn = tubules; Red = actin

Complexity of the Cytoplasm and Cytoskeleton

V

Unhindered Motion of Myosin Motors

Goldman, Bau, Arsenaut

Arsenault, M., Zhao, H., H., Purohit, P., Goldman, Y., and Bau, H. H., 2007, Biophysical Journal 93, L42-L44

Glass

ActinGold Electrode

1 m, Myosin-Coated Bead

~2 m

Increased Complexity in Protein Environment

Goldman, Bau, ShumanScience 2008J. Biophysics 2009,

Whole Lotta Shakin’

Drosophila S2 cell with microtubules shown in red and peroxisomes in green.

Recently developed imaging technology allows us to look inside living cells with unprecedented spatial and time resolution (nanometers and milliseconds). Extending this method to two colors allowed us to perceive previously hidden relations between cell components. For example, our work documented correlation between the motions of distant call cargos, indicating that they were both attached to the same active structural element (a microtubule).

IM Kulic and PC Nelson, Europhys Lett 2007; IM Kulić, AEX Brown, H Kim, C Kural, B Blehm, PR Selvin, PC Nelson and VI Gelfand, The role of microtubule movement in bidirectional organelle transport. PNAS 2008.

We have exquisite information about the static structures of cellular components, but still little understanding of how they orchestrate their many dynamic functions. The understanding now beginning to emerge will influence cell biology over the coming years.

Scientific OpportunitiesBiomolecule platforms and Hybrid nanostructures offer pathways to engineered function

pathogen detection protein sensorsoptoelectronic devicesenergy harvesting strategies

Single molecule studies in the context of realistic environments reveal complex behavior

cell division (cancer) disease targetscell motion disease targetslive proteomics: in situ protein synthesis

Probes of nanoscale phenomena are poised for another revolution