development of an integrated genomatica biofuel and ...• xum (xylose utilizing mutant) for xylose...
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Genomatica Sustainable Chemicals
Genomatica Sustainable Chemicals
Development of an Integrated Biofuel and Chemical Refinery
John D. Trawick
Research Fellow, Genomatica
2013 DOE Bioenergy Technologies Office (BETO) Project Peer Review
Date: 21 May 2013
Technology Area Review: Biochemical Conversion
Principal Investigator: Mark Burk Organization: Genomatica
This presentation does not contain any proprietary, confidential, or otherwise restricted information
Goal Statement Demonstrate the viability and commercial readiness of an integrated
biorefinery for low cost production of 1,4-butanediol (BDO), from biomass—deliver the engineered strain and optimized fermentation
process to enable the conversion of cellulosic sugars into BDO.
1: Improving the microbial conversion of cellulosic sugars to BDO.
To deliver commercially acceptable performance and enable scalable integrated biorefineries.
2: Characterizing and improving tolerance to cellulosic hydrolysate.
To deliver commercially acceptable performance and enable scalable integrated biorefineries.
3: Developing and optimizing a scalable fermentation process. Demonstrate the feasibility and scalability of integrated biorefineries.
To deliver a scalable fermentation process that employs the engineered microbe to produce BDO from cellulosic sugars at titer ≥ 70 g/L, and productivity ≥ 2.0 g/L/hr at ≥ 100 L scale.
Quad Chart Overview
• August 2011 • July 2014 • ~80% complete
• Barriers addressed • Consistency, quality, and concentration of
cellulosic sugars in hydrolysates. • Glucose – Xylose – Arabinose co-utilization • BDO T-R-Y metrics in hydrolysates vs.
refined sugar
Funding for FY11(541,971/186,308) Funding for FY12(852,555/293,074) Funding for FY13 (253,588/87,173) 2/ $1,000,000.
Timeline
Budget
Barriers
• Chemtex • Suppliers of PROESATM hydrolyates, have worked
with Genomatica to reach a specification • Biweekly or more frequent consultation with
Chemtex staff
Partners
Project Overview Genomatica has developed recombinant organisms to produce the commodity, 1,4-butanediol (BDO), used in many synthetic polymers.
•BDO producing strains use refined dextrose to make BDO; ties BDO economics to sugar and corn prices.
•Biomass-based sugars are an economical alternative.
•Challenges include:
o Cheap, consistent feedstock and treatment.
o Minimizing hydrolysate impurities.
o Achieving organism performance metrics: titer (T), rate (R), and yield (Y) similar to dextrose-based.
o Simultaneous utilization of C5 as well as C6 sugars by recombinant Escherichia coli.
o Recovery of BDO is different than ethanol and this impacts design and economics.
1 - Approach • E. coli to make 1,4-BDO at titer, rate, and yield from lignocellulosic
biomass sugars to demonstrate commercial feasibility.
• Critical factors: 1) hydrolysate quality/composition, 2) C5-C6 co-utilization, 3) process impacts on yield and rate.
• Hydrolysate composition, work with supplier to produce biomass hydrolysates with high and uniform [sugar], low impurities, and minimal toxicity.
– Go/no go: achieve 75% T-R-Y of pure glucose at same concentration.
• Adaptive evolution + genomic re-sequencing for sugar co-utilization.
– Go/no go: Efficient C5-C6 co-utilization and move to ‘clean’ strain
• 13C flux analysis + metabolomics to ID metabolic constraints limiting performance.
– Targets to improve energy and reduced cofactors.
Bio-based 1,4-Butanediol
Crude Oil & Nat. Gas
Acetylene
Butane, Butadiene
Propylene
2.8 B lb/yr existing market
spandex polyurethanes
PBT
Sugars
Direct Production
Genomatica’s Systems-Based Strain Engineering
Whole Cell Mutagenesis
Metagenomics, Enzyme Evolution
Computational Technologies
Pathway &
Strain Design
Product Feedstock Organism & Tools Select
Parent strain
Engineered strains
HT Screening In vivo assays
Metabolic Engineering Tools HT Cloning
Dat
a
LIMS Fermentation development/ scale-up
Omics data Systems analysis
Analyze & Interpret
13C-Fluxomics
Metabolomics
Proteomics
Transcriptomics
Genomics
Iterative Strain
Engineering
Biological
Knowledge
Journey to a BDO Production Strain
Pathway Identification and Engineering
glucoseex
acetyl-CoA citrate
phosphoenolpyruvate
glyoxylatemalate
pyruvate
ADP, NAD+
ATP, NADH
NAD(P)HCO2
NADH CO2
NAD+
ATPCoA
ADPPi
succinate
oxaloacetate
succinyl-CoA
fumarate
NAD+
NADH,CO2
CO2
hexose-P
-ketoglutarate
NAD(P)+
CoA
succinatesemialdehyde
CO2 NAD(P)+NAD(P)H
isocitrate
ATP
ADP
4-hyd
roxy
butyrate
4-hyd
roxy
butyrylC
oA
acetate
AMP
quinol
quinone
4-hydroxybutyryl-aldehyde
1,4-butanediol
1,4-butanediolex
NAD(P)+
NAD(P)H
NAD(P)+
, CoA
ATP, CoA
NAD(P)H
sucC
D
sucA
sucD
4hbd
ald
adh
PTS system
ppc
ATPADP
sucroseex
glk
ATP
ADP
frk
NAD(P)+
NAD(P)H
acs
sucAB, lpdA
lpdA
acetyl-CoA
mqoicdA
fumABC
sdhABCD
acn
aceAaceB
gltA
ubiquinone
ubiquinol
pckA
ADPCO2
ATP
cat2
renewable feedstock
sustainable chemicals
mdh
arcA
ldhadhEpflB
K.p.lpdD354KlpdA
gltAR163L
rrnC::cscAKB
Strain Design and Metabolic Engineering
Commercial Strain for BDO Production
• Titer (g/L) - Impacts equipment sizing and energy needs • Rate (g/L/h) - Impacts # of fermentors, plant capacity • Yield (g/g) - Impacts feedstock cost contribution
Fermentation Metrics → Higher TRY = Lower COGS
TRY all inter-dependent → reduce by-products, increase rate and yield
BDO Biosynthetic Pathways
Alternative routes
1. Developed enzyme assays and analytical methods for all metabolites 2. Screened libraries of gene candidates for each step – >100 in some cases 3. Demonstrated seven different functional BDO pathways in E. coli
Sugar
• Prioritized pathways proceed through 4-hydroxybutyrate • Downstream enzymes function on non-natural substrates
ALD Cat2 ADH
Yim et al., Nature Chem. Biol., 2011
BDO Pathway and Process
• BDO pathway involves 4 reduction steps – redox intensive
• BDO pathway generates 1 extra NAD(P)H and no excess ATP
• Balance energy, redox and maintain high NAD(P)H/NAD(P)+ ratio
• Microaerobic production (DO ≈ 0) required for optimal performance
ATP = 0 NAD(P)H = +1
Oxidative TCA cycle flux required for redox needs of BDO pathway
ATP via oxidative phosphorylation
E. coli
Glycolysis
TCA Cycle
BDO Pathway
BDO
E. coli
Sugars Glycolysis
TCA Cycle
BDO Pathway
C6H12O6 + 0.5 O2 C4H10O2 + 2 CO2 + H2O Max yield = 1 mol/mol (0.50 g/g, 67 C-mol %)
>100 g/L
Technology Platform Drives BDO Strain Performance
3.3 g/L-h 2.5 g/L-h 2.0 g/L-h
2011
2012
Increasing Rate and Lowering By-products
More metabolic steps in a pathway increases avenues for by-products
Key Advances • Backflux • Enzymes • Redox • ATP supply • Regulation • Balanced expression • Fermentation PD
2 PEP
PYR
Ace ACCOA OA
G6P
CIT
ICIT
AKG
SUCSAL
4HB
4HBALD
BDO
+ATP
-ATP
+NADH
+NADPH
- NADH
- NAD(P)H
+2 NADH
- NAD(P)H
1 GLUCOSE
CO2 loss
Acetate
Glutamate
CO2 loss via oxidative TCA
4-HB
Ethanol -2 NADH
SUCCOA
TCA Cycle
GBL
+ATP
Metabolic Engineering Applications
Approach to Biomass - BDO • Initial baseline performance (preliminary results in the grant).
• Identification of key constraints on Biomass – BDO and overcoming those constraints
o Hydrolysate composition, work with supplier to produce biomass hydrolysates with high and uniform [sugar], low [impurities], and minimal toxicity. o Hydrolysate improvements with time increased performance.
o Adaptive evolution + genomic re-sequencing + strain engineering for sugar co-utilization. o Acceptable sugar co-utilization selected, designed, and recapitulated.
o 13C flux analysis + metabolomics to ID metabolic constraints limiting performance. o Targets to improve availability of energy and reduced cofactors.
Benchmark performance on biomass, September 2011
• Benchmark fermentations for DOE/NREL site visit using early lot of hydrolysate and a very early BDO production strain*.
• Titer (16 – 18 g BDO/L) was 24% of grant goal and rate (0.25 g/L/hr) <10% of goal.
• Yield (not shown) >50% of goal.
• A long way to go!
*As used in grant application.
Biomass-to-BDO Process Challenges: impurity reduction
35% Glucose 007, Chemtex Hz, low impurities 001, Chemtex Hz, diluted w/sugar 001, Chemtex Hz, untreated Benchmark, 9/’11, early strain, Hz similar to 001 Strain: BDO Producer Chemtex Hydrolysate at 35% sugars in feed: • Lot 001, Untreated Hz • 001, Untreated Hz
Diluted with pure glucose + xylose
• Lot 007: Hz
Chemtex provided hydrolysates to evaluate •Sugar concentration in a fed-batch process—limits titer and rate •Lowered impurities; reduced multiple ways
Result: BDO titer approached metric on pure glucose Strain improvements from original benchmark coupled with Hz improvements.
BDO titer (g/L)
Biomass-to-BDO Process Challenges : Impurity reduction
35% Glucose Chemtex Hz, low impurities Chemtex Hz, diluted w/sugar Chemtex Hz, untreated Benchmark, 9/’11, early strain, Hz similar to 001 Strain: BDO Producer ChemtexHydrolysate at 35% sugars in feed: •Untreated •Diluted with pure glucose + xylose •Low impurities
Chemtex provided hydrolysates to evaluate •Sugar concentration in a fed-batch process—limits titer and rate •Lowered impurities; reduced multiple ways
Result: BDO titer approached metric on pure glucose Strain improvements from original benchmark coupled with Hz improvements.
BDO rate (g/L/hr)
Escherichia coli BDO production strains show diauxic sugar utilization
Compared, wt MG1655 vs. a BDO production strain 50/50 glucose xylose, growth curves
Conclusion: BDO production strains have lessened diauxie
relative to the wt progenitor.
0.000
0.100
0.200
0.300
0.400
0.500
0.600
0.700
0
2.2
5
4.5
0
6.7
5
9.0
0
11
.25
13
.50
15
.75
18
.00
20
.25
22
.50
24
.75
27
.00
29
.25
31
.50
33
.75
36
.00
38
.25
40
.50
42
.75
45
.00
0.1% glucose + 0.1% xylose2731/pZE13
0.1% glucose + 0.1% xylose MG1655
Using Evolution to Improve Xylose Sugar Utilization in Fermentation
Parent unevolved parent BDO producing strain.
Evolved : evolved from Parent for improved xylose utilization. Fermentation used pure glucose : xylose (1:1), 680 g sugar/L feed
Selection gave a large improvement in xylose consumption in the presence of glucose
• Fermentation in 2 L BR • Strains made BDO in glucose + xylose;
• Evolved = 70 - 75 g/L; • Parent = 60 g/L
0%
20%
40%
60%
80%
100%
0 10 20 30 40 50
% G
luc/
Xyl
C
on
sum
ed
Time (hrs)
%Glucose/Xylose Consumption
Evolved Glucose Cons
Evolved Xylose Cons
Parent Glucose Cons
Parent Xylose Cons
0
40
80
120
160
200
240
0 10 20 30 40 50
Tota
l Glu
c/X
yl C
on
sum
ed
(g)
Time (hrs)
Total Glucose/Xylose Consumption
Evolved Glucose Cons
Evolved Xylose Cons
Parent Glucose Cons
Parent Xylose Cons
Evolved and re-capitulated for glucose + xylose co-utilization
• XUM (xylose utilizing mutant) for xylose co-utilization with glucose was
identified and integrated into a clean BDO production host. • A gene for arabinose uptake also added. Result: Efficient co-utilization of all 3 sugars during fermentation.
0.00
20.00
40.00
60.00
80.00
0 8 13 18 23 28 33
Re
sid
ual
Su
gar
(mM
)
Elapsed time (hours)
C6 - C5 Co-Consumption
Glucose
Xylose
Arabinose
Residual [arabinose] in non-arabinose utilizing
strains with similar biomass hydrolysates for
comparison.
Improving BDO Performance on lignocellulosic hydrolysates with strain engineering and hydrolysate characteristics
ID C u ltu re ID Eq u ip Start Date In i t OD Tite r P ro d Yie ld C arb o n B alan ce
IR R Time p o in t Yie ld B asis C ate go ry Varyin g C o mp o n en t
LIM S ID Strain Name Strain Al t Name
No me n clatureP lasmid R e vie we d Ou tl ie r
1 4 8 7 -1 B R 1 7 4 /1 0 /2 0 12 1 2 :0 0 :00 AM
3 .9 2 3 9 .2 0 3 0 .8 9 1 0 .2 4 2 8 5 .8 3 7 6 .8 4 4 [Ara], [Glu c], [X yl ]
P ro ce ss De ve lo p men t
1 0 % M &G WS Hyd ro lysate , M M 9 , 2 mM
M gSO4, 2x TE , M &G WS Hyd ro lysate C e n tri fu ged an d Fi l te re d
(0 0 1-12-WS-4X)
L4 3 2 1 EC K h -3 9 33 + p ZS* 1 3S-dBsaI-7 1 4 B K -143 8A
Ed n a p ZS* 1 3S-dBsaI-7 1 4 B K -143 8A
N N
2 6 8 9 -1 B R 2 9 /2 5 /2 0 12 1 2 :0 0 :00 AM
5 .6 8 7 6 .7 9 1 .6 0 .4 8 6 1 5 5 .8 82 1 7 .5 4 8 [Ara], [Glu c], [X yl ]
Strain Scre e n in g
FM 8 - 3 .65 % M &G Lot 012 , M &G-0 1 2-WS-
NH3 , Raptor 1 o n Lo t 0 12
L5 8 4 1 R ap to r 1 re tran sfo rmed
R ap to r 1 re tran sfo rmed
N N
3 7 6 9 -1 B R 1 1 1 2 /4 /2 0 12 1 2 :0 0 :00 AM
3 .6 8 9 .1 1 5 1 .7 3 0 .3 9 3 1 2 0 .2 33 1 6 .3 2 5 1 .5 [Ara], [Glu c], [X yl ]
Strain Scre e n in g , M e d ia
De ve lo p men t
FM 2 5 - 2 .88% M &G Lot 010 , M &G-0 1 0 ,12-
WS-S-NH3 , Lot 0 1 0
L6 2 0 2 EC K p -5 1 57 + p ZS* 1 3 S-p246-1 4 3 8 -14 39-
1 6 0 B-p 115 -3 0 1 -3 02
EC K s-5 4 1 1 p ZS* 2 3 S-p246-1 4 3 8 -14 39-1 6 0 B -p 115 -
3 0 1 -3 02
N N
• Better strains and biomass treatment = improved BDO titer and rate
• Early 2012 diauxic strain, early Hz, Chemtex
• Late ‘12, Evolved/recapitulated xylose user, Chemtex, low impurities
• Late ‘12, Evolved/recapitulated xylose user, Chemtex, low impurities
• >2X in increase in titer, and in peak rate during 2012
• Challenges remain • Consistency/sugar concentration • Reducing non-fermentables
Non-evolved strain Chemtex Hz-001, early batch Recapitulated, XUM Chemtex Hz-012, improved Recapitulated, XUM Chemtex Hz-010, improved
BDO titer (g/L) Final titer, 89 g
BDO/L
Final titer, 85 g BDO/L
Final titer, 39 g BDO/L
BDO rate (g/L/hr)
Cellulosic biomass (xylose) and metabolism
ADP
sucroseex
ATP
frk
acetyl-CoA
citrate
phosphoenolpyruvate
glyoxylatemalate
pyruvate
NADPHCO2
NADH CO2
NAD+
ATPCoA
ADPPi
succinate succinyl-CoA
fumarate
NAD+
NADHCO2
CO2
-ketoglutarate
NAD+
CoA
succinatesemialdehyde
glutamate4-aminobutyrate NAD+NADH
isocitrate
4-hydroxybutyrate
4-hydroxybutyrylCoA
g-butyrolactone
acetateCO2
ADP
CoAquinol
quinone
4-hydroxybutyrylaldehyde
1,4-butanediol
1,4-butanediolex
NAD(P)+
NAD(P)H
NAD(P)+
, CoA
ATP, CoA
NAD(P)H
sucC
D sucD (035)
4hbd (036)
cat2 (033U)
ald ( 714B)
adh (956)
ppc
mdh
acetyl-P
NADP+
NADH
ack,pta
sucAB, lpdA
lpdA
acetyl-CoA
1,4-Butanediol Biosynthetic Pathway
mqo
NAD(P)HCO2
NADP+
sfcA,maeB
icdA
fumABC
sdhABCD
R163Lacn
aceAaceB
gltA
ubiquinone
ubiquinol
pepck
ADPCO2
ATP
Confidential
aspAaspartate
aspC
glutamate-ketoglutarate
car (891)
NADP+
NADPH
±
±
+ pptase (910)
glutamateNADP+
NADPH
gdh
glutamateex
gltBD
glucoseex
ADP, NAD+
ATP, NADH
fructose6-P
ADP
PTS system
ATPADP
glk
fructose 1,6-P
fbp pfkABADP
ATPPi
pgi
glucose 6-P
glyceraldehyde 3-PfbaAB
PEP
pyruvate
pykAF
zwf
ATP
esterase
4-hydroxybutyrateex
ribulose 5-P
xylulose 5-P
ribose 5-P
sedoheptulose 7-P
erythrose 4-P
NADPHNADP+
ripAB rpe
tktAB
tktAB
talB
NADPH,CO2NADP+
gnd
pgl
6P-gluconolactone
6P-gluconate
gabT
gadAB
xylB
xylA
xyloseex
xylulose
xylosexylEFGH
sucroseex
glucose
fructose
permease
invertase
ADP
ATP
CoAPi
pta
acetate
ATP
ADP
acetateex
ackA
oxaloacetate
13C flux analysis, metabolomics, and metabolic modeling used to ID energy
and metabolic constraints on BDO production in hydrolysates with mixed
sugars.
Including,
Net ATP reduced on xylose because of increased transport cost from xylFGH ABC
transporter
Key Accomplishments and challenges • Improved hydrolysate composition—addresses impurities, sugar
concentrations
• Glucose – Xylose – Arabinose co-utilization
• These and other changes have given at small scale fermentation:
o Chemtex hydrolysates: 86 – 89 g BDO/L titer, 1.8 g/L/hr rate
o DOE grant goal (30L scale): 70 g BDO/L titer, 1.5 g/L/hr rate
• Potential drains on energy and reducing power identified via metabolomics, 13C flux analysis, and metabolic modeling.
• Have multiple proposed changes in process of testing/implementation
3 - Relevance • Develop biomass-to-1,4-butanediol (BDO) increase chemical production
from sustainable feedstocks in a cost advantaged process that will be competitive with petrochemicals
o Achieve performance sufficient for commercial viability (BDO titer of ≥ 70 g/L, and rate ≥ 2.0 g/L/hr) Grant Tasks 1 & 2
o Scalable process to integrate into biomass based biorefineries (at ≥ 100 L scale) Grant Task 3
o Recovery of BDO along with process that is cost competitive. Grant Tasks 1, 2, & 3.
• Application of the expected outputs: Bio-BDO from cellulosic biomass, cost advantaged, that can be incorporated into the integrated biorefinery concept.
4 - Critical Success Factors • Success factors defining technical and commercial viability.
BDO titer of ≥ 70 g/L, and rate ≥ 2.0 g/L/hr at ≥ 100 L scale. o Robust organism meeting these targets (technical); >75% of the goal o From biomass, BDO purity equal to industry needs (market); can do. o Costs comparable or less than pure glucose “Gen 1 BDO” process (business);
working towards this with improved strains, hydrolysates, process.
• Potential challenges (technical and non-technical). o Cellulosic hydrolysate consistency/specs for optimum fermentation and
downstream recovery. o Strain performance in hydrolysates, especially energy cost to cell affecting yield
and productivity.
• Advance the technology and impact the commercialization of biomass/biofuels. o BDO strain/process/recovery are all distinct from ETOH or biofuels; will be a new
opportunity. o Commercially viable strain and process, biomass-to-BDO, will be very attractive to
Genomatica customers. o Begin to set specifications for biomass sugars for chemical processes
5. Future Work • Improve yield and incorporate sugar co-utilization into multiple strains. • Go/no go reaching T-R-Y targets at both 2 L and larger scale • Test new lignocellulosic hydrolysate feedstocks and treatments.
8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7
= Deliverables = Major Stage Gates
Project Month 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36
1 Improve the microbial conversion of cellulosic sugars to BDO
1.1 Maximize overall sugar utilization rate 1.1.1 Eliminate diauxic sugar utilization 1.1.2 Improve individual sugar utilization rates 1.1.3 Enable breakdown of cellobiose 1.2 Maximize BDO yield 1.2.1 Balance BDO pathway expression/flux with upstream metabolism 1.2.2 Reduce yield loss to overflow byproducts
2 Characterize and improve tolerance to cellulosic hydrolysate
2.1 Characterize and evolve tolerance to hydrolysate 2.1.1 Determine maximum tolerance to hydrolysate 2.1.2 Evolve wild-type and BDO production organism for improved tolerance 2.1.3 Characterize/resequence evolved organisms 2.2 Develop hydrolysate-tolerant production strain 2.2.1 Evaluate BDO production capabilities of evolved ‘production’ strain 2.2.2 Introduce observed mutations into unevolved production strain and
evaluate hydrolysate tolerance/BDO production
3 Develop fermentation process that consistently produces BDO from
cellulosic sugars at the target titers, yields, and productivities
3.1 Develop efficient and scalable fermentation process 3.1.1 Establish new baseline with latest BDO production strain 3.1.2 Determine maximum starting hydrolysate concentration 3.1.3 Perform initial media and protocol optimization 3.1.4 Determine effective fed-batch feeding strategy 3.1.5 Perform process development with emphasis on oxygen supply 3.2 Test improved strains and modify fermentation process as needed 3.3 Complete technology transfer package
2012 2013 2014
Task Description 2011
40 g/L, 0.75 g/L/hr, 2 - L scale
70 g/L, 1.5 g/L/hr, 30 - L scale
70 g/L, 2.0 g/L/hr, 100 - L scale
Simultaneous C5 - and C6 - sugar utilization achieved.
Growth of evolved strains in 180 g/L hydrolysate demonstrated . Strain resequencing complete.
Production strain tolerates 180 g/L hydrolysate.
Sugar utilization exceeds 4 g/L/hr. Yield goal at 2 L scale. Cellobiose utilization demonstrated.
Sugar utilization exceeds 5 g/L/hr. ? 0.42 g/g achieved at 2 - L scale.
Summary 1) Approach
1) Strain design and engineering coupled with adaptive evolution and ‘omics technologies to ID key constraints on feedstock quality and price.
2) Hydrolysate evaluation and improvement to reach specification.
2) Accomplishments 1) 5X improvement in titer; rate and yield are approaching goals.
2) Major C6 and C5 sugars can be co-utilized to max yield and performance
3) Key metabolic constraints ID’d
4) Working towards hz specification
3) Relevance 1) Enable commercial bio-BDO from lignocellulosic biomass sugars
4) Critical Success factors and challenges 1) Biomass sources meeting needed fermentation and BDO recovery specifications.
5) Future Work 1) Yield and pathway enhancements to reach or exceed grant metrics.
6) Technology transfer
Bio-BDO commercial progress
50 runs 120+ trucks
BASF, world’s leading BDO producer licensed to produce renewable 1,4-BDO using Genomatica’s process
Toray Industries, Inc. produced partial bio-PBTs from bio-BDO at same specs as petro-PBT
Bio-BDO Scale-up, 3 yrs to 5 M-lbs
Genomatica Biomass to BDO Contributors Molecular Biology
John Trawick
Ewa Lis
Joseph Warner
Chemtex
Alberto Ceria
Piero Ottonello
Francesco Cherchi
Kevin Gray
Microbiology
Carla Risso
Jesse Wooton
Jonathan Joaquin
Analytical Sciences
Julia Khandurina
Rosary Stephen
Lucy Zhao
Ahmed Alanjary
Blanca Ruvalcaba Rainer Wagester Korki Miller
Process Engineering
Michael Japs
Janardhan Garikipati
Fasil Tadesse
Matt White
Christophe Schilling, CEO Mark Burk, CTO Bill Baum, CBO Nelson Barton, VP R&D Jeff Lievense, EVP, Process Development
Computational
Tony Burgard
Priti Pharkya
Robin Osterhout
Jun Sun
Tae Hoon Yang
Jungik Choi
Harish Nagarajan
Fermentation
Dan Beacom
Sy Teisan
Laurie Romag
Joseph Woodcock
Gian Oddone
Amruta Bedekar
Jason Crater
Akhila Raya Award DE-EE0005002 to Genomatica
Questions?
Thank you John Trawick [email protected]
Publications, Presentations, and Commercialization
• Barton, Nelson (VP, R&D, Genomatica) Biomass 2012, 11 – 12 July 2012, Wash., DC http://www1.eere.energy.gov/biomass/pdfs/bio2012_final_agenda.pdf
• Trawick, John D. (Research Fellow, Genomatica) scheduled for the 2013 SBFC meeting (2 May 2013) http://sim.confex.com/sim/35th/webprogram/Session2437.html
Note: This slide is for the use of the Peer Review evaluation only – it is not to be presented as part of your oral presentation, but can be referenced during the Q&A session if appropriate. These additional slides will be included in the copy of your presentation that will be made available to the Reviewers and to the public.
Responses to Previous Reviewers’ Comments
• N/A.
Note: This slide is for the use of the Peer Review evaluation only – it is not to be presented as part of your oral presentation, but can be referenced during the Q&A session if appropriate. These additional slides will be included in the copy of your presentation that will be made available to the Reviewers and to the public.