TO DETERMINE THE CONCENTRATION OF VITAMIN C CONTENT IN THE SAMPLE OF FRUIT JUICES Problem statement What is the sample of fruit juices that contains a higher concentration of vitamin C? Hypothesis Guava juice contains a higher concentration of vitamin C compared to orange Juice and pineapple juice Variables MV : type of fruit juice RV : concentration of vitamin C CV : volume of DCPIP solution Apparatus and materials Boiling tube,a syringe,a syringe with needles ,beaker,gauze cloth and a knife ,DCPIP solution,0.1% ascorbic acid solution .freshly prepared guava juice,pineapple juice and orange juice Procedure

1. Label four boiling tube as A,B,C, and D 2. Place 1ml of DCPIP solution in each boiling tube 3. Fill a syringe with 5ml of ascorbic acid solution 4. Immerse the needle of the syringe in the DCPIP solution drop-by-drop 5. Do not shake the tube vigorously 6. Record the volume of ascorbic acid solution used to turn the DCPIP solution colourless using

a syringe 7. Repeat steps 22 to 7 using Lime Juice,pineapple juice and papaya juice 8. Calculate the percentage and concentration of vitamin C in these three types of fruit juice

using the formula below [ shows percentage of vitamin C and Concentration of vitamin C formula]

Presentation of data

Solution Volume of fruit juice needed to decolourize 1ml of DCPIP solution

(ml)

Percentage of vitamin C In fruit juice (%)

Vitamin C concentration in fruit

juice (mg/cm)