isolation of herbal vitamin c - pharmacy mahidol u. special project submitted in ... **department of...
TRANSCRIPT
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ISOLATION OF HERBAL VITAMIN C
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MISS SUCHADA NILKAMHANK
MISS ANOCHA PAOCHOM
A SPECIAL PROJECT SUBMITTED IN
PARTIAL FULLFILMENT
OF THE REQUIREMENT FOR
THE BACHELOR DEGREE OF SCIENCE IN
PHARMACY
FACULTY OF PHARMACY
MAHIDOL UNIVERSITY
2000
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Abstract
Isolation of herbal vitamin C
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Suchada Nilkamhank, Anocha Paochom
Project adviser: Wandee Kritsanapun*, Leena Soonthonsuk**
*Department of Pharmacognosy, Faculty of Pharmacy, Mahidol
University
**Department of Pharmaceutical Chemistry, Faculty of Pharmacy,
Mahidol Univercity
Iodometry method was employed for determination of
vitamin C in Thai medicinal plants. The method was reliable
and validated with the percent recovery of 98.0 -105.0 %. The
precision of the method calculated as percent relative standard
deviations (%RSD) was 0.7-1.5% and the linearity (R2) was
0.9999-1.0000. The method offered good limit of detection and
limit of quantitation, which were 2.2 and 7.3 mg, respectively.
The method was used to determine vitamin C content in guava
(Psidium guajava Linn.), emblic myrobolan (Phyllanthus
embica Linn.), lemon (Citrus aurantifollia Swing.), sweet
pepper (Capsicum annuum Linn.), Garcinia schomburgkiana
Pierre., passion fruit (Passiflora laurifoia Linn.). The amount of
vitamin C found were guava 80.1, emblic myrobolan 226.0,
sweet pepper 52.8, passion fruit 39.1, lemon 10.5, Garcinia
schomburgkiana 4.6 mg /100 g, respectively. Emblic
myrobolan and guava juice, which contained highest Vitamin C,
were freeze–dried and re-analyzed. Vitamin C in both freeze–
dried samples decreased to 46.9 and 179.8 mg/100 g,
respectively. Stability of the freeze–dried samples was examined
by keeping the samples in a dessicator and the vitamin C content
was re-analyzed during the first and second month of the
storage. Results showed that after one month, vitamin C in
guava and emblic myrobolan juice were 33.8 and 172.7 mg/100
g, respectively and after two month traces of Vitamin C was
still existed when detected by TLC comparing to the standard.